UniProtKB - A4IFH6 (PPLA_BOVIN)
Protein
Cardiac phospholamban
Gene
PLN
Organism
Bos taurus (Bovine)
Status
Functioni
Reversibly inhibits the activity of ATP2A2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca2+. Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation (By similarity).By similarity
GO - Molecular functioni
- ATPase inhibitor activity Source: InterPro
- protein homodimerization activity Source: UniProtKB
GO - Biological processi
Names & Taxonomyi
Protein namesi | Recommended name: Cardiac phospholambanShort name: PLB |
Gene namesi | Name:PLN |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Subcellular locationi
Mitochondrion
- Mitochondrion membrane 1 Publication; Single-pass membrane protein Sequence analysis
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Single-pass membrane protein Sequence analysis
Other locations
- Sarcoplasmic reticulum membrane By similarity; Single-pass membrane protein Sequence analysis
- Membrane By similarity; Single-pass membrane protein Sequence analysis
Note: Colocalizes with HAX1 at the endoplasmic reticulum. Colocalizes with DMPK a the sarcoplasmic reticulum.By similarity
Endoplasmic reticulum
- sarcoplasmic reticulum membrane Source: UniProtKB
Mitochondrion
- mitochondrial membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 30 | CytoplasmicSequence analysisAdd BLAST | 30 | |
Transmembranei | 31 – 51 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Endoplasmic reticulum, Membrane, Mitochondrion, Sarcoplasmic reticulumPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000353186 | 1 – 52 | Cardiac phospholambanAdd BLAST | 52 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionine1 Publication | 1 | |
Modified residuei | 16 | Phosphoserine; by PKA and DMPKBy similarity | 1 | |
Modified residuei | 17 | Phosphothreonine; by CaMK2By similarity | 1 | |
Lipidationi | 36 | S-palmitoyl cysteineBy similarity | 1 |
Post-translational modificationi
Phosphorylation by PKA abolishes the inhibition of ATP2A2-mediated calcium uptake. Phosphorylated at Thr-17 by CaMK2, and in response to beta-adrenergic stimulation. Phosphorylation by DMPK may stimulate sarcoplasmic reticulum calcium uptake in cardiomyocytes (By similarity).By similarity
Palmitoylated by ZDHHC16, promoting formation of the homopentamer.By similarity
Keywords - PTMi
Acetylation, Lipoprotein, Palmitate, PhosphoproteinProteomic databases
PaxDbi | A4IFH6 |
PTM databases
iPTMneti | A4IFH6 |
Interactioni
Subunit structurei
Homopentamer.
Interacts with HAX1 and ATP2A2 (By similarity).
By similarityGO - Molecular functioni
- protein homodimerization activity Source: UniProtKB
Protein-protein interaction databases
CORUMi | A4IFH6 |
STRINGi | 9913.ENSBTAP00000017182 |
Family & Domainsi
Sequence similaritiesi
Belongs to the phospholamban family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502S97F, Eukaryota |
InParanoidi | A4IFH6 |
OrthoDBi | 1643351at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR005984, PLB |
PANTHERi | PTHR21194, PTHR21194, 1 hit |
Pfami | View protein in Pfam PF04272, Phospholamban, 1 hit |
PIRSFi | PIRSF001665, PLB, 1 hit |
TIGRFAMsi | TIGR01294, P_lamban, 1 hit |
i Sequence
Sequence statusi: Complete.
A4IFH6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDKVQYLTRS AIRRASTIEM PQQARQNLQN LFINFCLISI CLLLICIIVM
LL
Mass spectrometryi
Molecular mass is 6122.7 Da. Determined by ESI. 1 Publication
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC134584 mRNA Translation: AAI34585.1 |
RefSeqi | NP_001096789.1, NM_001103319.1 |
Genome annotation databases
GeneIDi | 100125240 |
KEGGi | bta:100125240 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC134584 mRNA Translation: AAI34585.1 |
RefSeqi | NP_001096789.1, NM_001103319.1 |
3D structure databases
SMRi | A4IFH6 |
ModBasei | Search... |
Protein-protein interaction databases
CORUMi | A4IFH6 |
STRINGi | 9913.ENSBTAP00000017182 |
PTM databases
iPTMneti | A4IFH6 |
Proteomic databases
PaxDbi | A4IFH6 |
Genome annotation databases
GeneIDi | 100125240 |
KEGGi | bta:100125240 |
Organism-specific databases
CTDi | 5350 |
Phylogenomic databases
eggNOGi | ENOG502S97F, Eukaryota |
InParanoidi | A4IFH6 |
OrthoDBi | 1643351at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR005984, PLB |
PANTHERi | PTHR21194, PTHR21194, 1 hit |
Pfami | View protein in Pfam PF04272, Phospholamban, 1 hit |
PIRSFi | PIRSF001665, PLB, 1 hit |
TIGRFAMsi | TIGR01294, P_lamban, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PPLA_BOVIN | |
Accessioni | A4IFH6Primary (citable) accession number: A4IFH6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 4, 2008 |
Last sequence update: | May 1, 2007 | |
Last modified: | December 2, 2020 | |
This is version 75 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families