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Protein

Bifunctional NAD(P)H-hydrate repair enzyme

Gene

LBRM_14_1360

Organism
Leishmania braziliensis
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Bifunctional enzyme that catalyzes the epimerization of the S- and R-forms of NAD(P)HX and the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. This allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration (By similarity).By similarity

Catalytic activityi

ADP + (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide adenine-dinucleotide = AMP + phosphate + NADH.
ADP + (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide adenine-dinucleotide phosphate = AMP + phosphate + NADPH.
(6R)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide = (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide.
(6R)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate = (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate.

Cofactori

K+By similarityNote: Binds 1 potassium ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi78PotassiumBy similarity1
Metal bindingi145PotassiumBy similarity1
Binding sitei160NAD(P)HX (for epimerase activity)By similarity1
Binding sitei178NAD(P)HX (for epimerase activity)By similarity1
Metal bindingi181PotassiumBy similarity1
Binding sitei351NAD(P)HX (for dehydratase activity); via amide nitrogenBy similarity1
Binding sitei485NAD(P)HX (for dehydratase activity)By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi454 – 458ADPBy similarity5
Nucleotide bindingi475 – 484ADPBy similarity10

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase, Lyase, Multifunctional enzyme
LigandATP-binding, Metal-binding, NAD, NADP, Nucleotide-binding, Potassium

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional NAD(P)H-hydrate repair enzyme
Alternative name(s):
Nicotinamide nucleotide repair protein
Including the following 2 domains:
ADP-dependent (S)-NAD(P)H-hydrate dehydratase (EC:4.2.1.136)
Alternative name(s):
ADP-dependent NAD(P)HX dehydratase
NAD(P)H-hydrate epimerase (EC:5.1.99.6)
Alternative name(s):
NAD(P)HX epimerase
Gene namesi
ORF Names:LBRM_14_1360
OrganismiLeishmania braziliensis
Taxonomic identifieri5660 [NCBI]
Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeLeishmaniinaeLeishmaniaLeishmania braziliensis species complex
Proteomesi
  • UP000007258 Componenti: Chromosome 14

Organism-specific databases

EuPathDBiTriTrypDB:LbrM.14.1360

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004164331 – 561Bifunctional NAD(P)H-hydrate repair enzymeAdd BLAST561

Interactioni

Protein-protein interaction databases

STRINGi420245.XP_001563422.1

Structurei

3D structure databases

ProteinModelPortaliA4H7T9
SMRiA4H7T9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini29 – 235YjeF N-terminalAdd BLAST207
Domaini249 – 548YjeF C-terminalAdd BLAST300

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 241NAD(P)H-hydrate epimeraseBy similarityAdd BLAST241
Regioni77 – 81NAD(P)HX (for epimerase activity)By similarity5
Regioni149 – 155NAD(P)HX (for epimerase activity)By similarity7
Regioni249 – 561ADP-dependent (S)-NAD(P)H-hydrate dehydrataseBy similarityAdd BLAST313
Regioni417 – 423NAD(P)HX (for dehydratase activity)By similarity7

Sequence similaritiesi

In the N-terminal section; belongs to the NnrE/AIBP family.Curated
In the C-terminal section; belongs to the NnrD/CARKD family.Curated

Phylogenomic databases

eggNOGiENOG410K31R Eukaryota
KOG2585 Eukaryota
COG0062 LUCA
COG0063 LUCA
InParanoidiA4H7T9
KOiK17758
K17759
OMAiLVGPGHN

Family and domain databases

CDDicd01171 YXKO-related, 1 hit
Gene3Di3.40.1190.20, 1 hit
3.40.50.10260, 1 hit
HAMAPiMF_01965 NADHX_dehydratase, 1 hit
MF_01966 NADHX_epimerase, 1 hit
InterProiView protein in InterPro
IPR017953 Carbohydrate_kinase_pred_CS
IPR000631 CARKD
IPR030677 Nnr
IPR029056 Ribokinase-like
IPR004443 YjeF_N_dom
IPR036652 YjeF_N_dom_sf
PfamiView protein in Pfam
PF01256 Carb_kinase, 1 hit
PF03853 YjeF_N, 1 hit
PIRSFiPIRSF017184 Nnr, 1 hit
SUPFAMiSSF53613 SSF53613, 1 hit
SSF64153 SSF64153, 1 hit
TIGRFAMsiTIGR00196 yjeF_cterm, 1 hit
TIGR00197 yjeF_nterm, 1 hit
PROSITEiView protein in PROSITE
PS01050 YJEF_C_2, 1 hit
PS51383 YJEF_C_3, 1 hit
PS51385 YJEF_N, 1 hit

Sequencei

Sequence statusi: Complete.

A4H7T9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLSRISERCT AVTGLEQVLC RHVWSAAWLR DAEPAAAASQ SIDLSCLMER
60 70 80 90 100
AGLAAYDVFA SLYASQRHWL ILVGSGNNGG DGYVIARHAR EAGRKVTVLS
110 120 130 140 150
MPHSKPLPTE ATNAQHAWQA VGGTETMINP GTSLHLPTDV DLVVDGLLGT
160 170 180 190 200
GISGPPREHY EEVIRHINAL PVPRVAIDIP SGLNAETGEA AGACVKADHT
210 220 230 240 250
ATFICLKPGL LTGQARDYVG QLHYRSLGLE EWMIAAERMD AALCRRVALG
260 270 280 290 300
DVYGYFSTRR SAVAHKGSCG KVVLIGGDHG FGGAILMSAE ACLTIGAGLT
310 320 330 340 350
RVLTRPEYVA PLLTRCPEVM VTAVETETSG QLEQQMVEAF EWASTLAVGP
360 370 380 390 400
GLGTGAYGQA ALSAALQQAE MHQDKTLVLD ADALNLLAER LHSKEMGAAV
410 420 430 440 450
GAGKYLPVLP NSIITPHPGE AARLLACRVA DVEKDRLAAA RRLAAILGGT
460 470 480 490 500
CLLKGPGTVV HCQSSGKTAI VDAGNAGMAS GGMGDVLTGL LAGLAAQRMM
510 520 530 540 550
HDTFDTTCAA ALVHGVAADM VAAEDGRGTR GIRATELIPR IPFIVNASVP
560
SSATQQRPSG L
Length:561
Mass (Da):58,658
Last modified:May 1, 2007 - v1
Checksum:iA6FB974C180B1B0D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FR798988 Genomic DNA Translation: CAM37606.1
RefSeqiXP_001563422.1, XM_001563372.1

Genome annotation databases

EnsemblProtistsiCAM37606; CAM37606; LBRM_14_1360
GeneDBiLbrM.14.1360:pep
GeneIDi5413933
KEGGilbz:LBRM_14_1360

Similar proteinsi

Entry informationi

Entry nameiNNR_LEIBR
AccessioniPrimary (citable) accession number: A4H7T9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 21, 2012
Last sequence update: May 1, 2007
Last modified: April 25, 2018
This is version 54 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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