UniProtKB - A4GTP4 (ELK1_RAT)
Protein
ETS domain-containing protein Elk-1
Gene
Elk1
Organism
Rattus norvegicus (Rat)
Status
Functioni
Transcription factor that binds to purine-rich DNA sequences. Forms a ternary complex with SRF and the ETS and SRF motifs of the serum response element (SRE) on the promoter region of immediate early genes such as FOS and IER2 (By similarity) (PubMed:17156131). Induces target gene transcription upon JNK-signaling pathway stimulation (PubMed:17156131).By similarity1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 5 – 86 | ETSPROSITE-ProRule annotationAdd BLAST | 82 |
GO - Molecular functioni
- chromatin binding Source: RGD
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: RGD
- DNA-binding transcription factor activity Source: RGD
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- double-stranded DNA binding Source: RGD
- nuclear receptor coactivator activity Source: Ensembl
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: RGD
- RNA polymerase II transcription factor binding Source: RGD
- sequence-specific double-stranded DNA binding Source: RGD
GO - Biological processi
- cell differentiation Source: GO_Central
- cellular response to gamma radiation Source: RGD
- cellular response to lipid Source: RGD
- cellular response to testosterone stimulus Source: RGD
- gene expression Source: RGD
- liver development Source: RGD
- lung development Source: RGD
- positive regulation of neuron death Source: RGD
- positive regulation of transcription, DNA-templated Source: RGD
- positive regulation of transcription by RNA polymerase II Source: MGI
- regulation of transcription, DNA-templated Source: RGD
- regulation of transcription by RNA polymerase II Source: GO_Central
- response to fibroblast growth factor Source: UniProtKB
- response to light stimulus Source: RGD
Keywordsi
Molecular function | DNA-binding |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: ETS domain-containing protein Elk-1Curated |
Gene namesi | Name:Elk1Imported |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 1598663, Elk1 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Mitochondrion
- mitochondrion Source: RGD
Nucleus
- nucleoplasm Source: Ensembl
- nucleus Source: RGD
Other locations
- axon terminus Source: RGD
- cytoplasm Source: RGD
- dendrite Source: RGD
- neuronal cell body Source: RGD
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000433929 | 1 – 427 | ETS domain-containing protein Elk-1Add BLAST | 427 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 229 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity | ||
Cross-linki | 248 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity | ||
Cross-linki | 253 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity | ||
Modified residuei | 323 | Phosphoserine; by MAPK1By similarity | 1 | |
Modified residuei | 335 | Phosphothreonine; by MAPK1By similarity | 1 | |
Modified residuei | 352 | Phosphothreonine; by MAPK1By similarity | 1 | |
Modified residuei | 362 | Phosphothreonine; by MAPK1By similarity | 1 | |
Modified residuei | 367 | Phosphothreonine; by MAPK1By similarity | 1 | |
Modified residuei | 382 | Phosphoserine; by MAPK1 and MAPK8By similarity | 1 | |
Modified residuei | 388 | Phosphoserine; by MAPK1By similarity | 1 | |
Modified residuei | 416 | Phosphothreonine; by MAPK1By similarity | 1 | |
Modified residuei | 421 | Phosphoserine; by MAPK1By similarity | 1 |
Post-translational modificationi
Sumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention.By similarity
On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-382 and Ser-388 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Also phosphorylated on Ser-382 by MAPK8 and/or MAKP9. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity. Phosphorylated and activated by CAMK4, MAPK11, MAPK12 and MAPK14 (By similarity). Upon bFGF stimulus, phosphorylated by PAK1 (PubMed:17156131).By similarity1 Publication
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
PaxDbi | A4GTP4 |
PTM databases
iPTMneti | A4GTP4 |
PhosphoSitePlusi | A4GTP4 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000010171, Expressed in skeletal muscle tissue and 20 other tissues |
Genevisiblei | A4GTP4, RN |
Interactioni
Subunit structurei
Interacts in its sumoylated form with PIAS2/PIASX which enhances its transcriptional activator activity.
Interacts with MAD2L2; the interaction is direct and promotes phosphorylation by the kinases MAPK8 and/or MAPK9.
Interacts with POU1F1.
By similarityGO - Molecular functioni
- RNA polymerase II transcription factor binding Source: RGD
Protein-protein interaction databases
CORUMi | A4GTP4 |
STRINGi | 10116.ENSRNOP00000013522 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 348 – 398 | Sufficient for interaction with MAD2L2By similarityAdd BLAST | 51 |
Sequence similaritiesi
Belongs to the ETS family.UniRule annotation
Phylogenomic databases
eggNOGi | KOG3806, Eukaryota |
GeneTreei | ENSGT00940000157571 |
HOGENOMi | CLU_036905_0_0_1 |
InParanoidi | A4GTP4 |
OMAi | NTAQVCG |
OrthoDBi | 981551at2759 |
PhylomeDBi | A4GTP4 |
TreeFami | TF317732 |
Family and domain databases
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR000418, Ets_dom IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF00178, Ets, 1 hit |
PRINTSi | PR00454, ETSDOMAIN |
SMARTi | View protein in SMART SM00413, ETS, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit |
PROSITEi | View protein in PROSITE PS00345, ETS_DOMAIN_1, 1 hit PS00346, ETS_DOMAIN_2, 1 hit PS50061, ETS_DOMAIN_3, 1 hit |
i Sequence
Sequence statusi: Complete.
A4GTP4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDPSVTLWQF LLQLLREQGN GHIISWTSRD GGEFKLVDAE EVARLWGLRK
60 70 80 90 100
NKTNMNYDKL SRALRYYYDK NIIRKVSGQK FVYKFVSYPE VAGCSTEDCP
110 120 130 140 150
PQPEVSVTSA VAMAPATVHS GPGDNATGKP GTPKGAGMTG QGGLARSSRN
160 170 180 190 200
EYMRSGLYST FTIQSLQPQP PLHPRPASVL PNTTPAGVPA PPSGSRSTSP
210 220 230 240 250
NPLEACLEAE EAGLPLQVIL TPPEAPNQKS EELSLNPGFG RPQPPEVKVE
260 270 280 290 300
GPKEELEVTE VGGFSPEAVK AEQEVSPSEG LLARLPAILT ENTAQVCGLS
310 320 330 340 350
TSTTEITQPQ KGRKPRDLEL PLSPSLLGGQ GPERTPGSGT SSGLQAQGPA
360 370 380 390 400
LTPSLLPTHT LTPVLLTPSS LPPSIHFWST LSPIAPRSPA KLSFQFPSSG
410 420
SAQVHIPSIS VDGLSTPVVL SPGPQKP
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | EF429098 mRNA Translation: ABO27185.1 AABR07036739 Genomic DNA No translation available. CH474009 Genomic DNA Translation: EDL97728.1 |
RefSeqi | NP_001101529.1, NM_001108059.3 XP_006256676.1, XM_006256614.2 |
Genome annotation databases
Ensembli | ENSRNOT00000013522; ENSRNOP00000013522; ENSRNOG00000010171 |
GeneIDi | 314436 |
KEGGi | rno:314436 |
UCSCi | RGD:1598663, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | EF429098 mRNA Translation: ABO27185.1 AABR07036739 Genomic DNA No translation available. CH474009 Genomic DNA Translation: EDL97728.1 |
RefSeqi | NP_001101529.1, NM_001108059.3 XP_006256676.1, XM_006256614.2 |
3D structure databases
SMRi | A4GTP4 |
ModBasei | Search... |
Protein-protein interaction databases
CORUMi | A4GTP4 |
STRINGi | 10116.ENSRNOP00000013522 |
PTM databases
iPTMneti | A4GTP4 |
PhosphoSitePlusi | A4GTP4 |
Proteomic databases
PaxDbi | A4GTP4 |
Genome annotation databases
Ensembli | ENSRNOT00000013522; ENSRNOP00000013522; ENSRNOG00000010171 |
GeneIDi | 314436 |
KEGGi | rno:314436 |
UCSCi | RGD:1598663, rat |
Organism-specific databases
CTDi | 2002 |
RGDi | 1598663, Elk1 |
Phylogenomic databases
eggNOGi | KOG3806, Eukaryota |
GeneTreei | ENSGT00940000157571 |
HOGENOMi | CLU_036905_0_0_1 |
InParanoidi | A4GTP4 |
OMAi | NTAQVCG |
OrthoDBi | 981551at2759 |
PhylomeDBi | A4GTP4 |
TreeFami | TF317732 |
Miscellaneous databases
PROi | PR:A4GTP4 |
Gene expression databases
Bgeei | ENSRNOG00000010171, Expressed in skeletal muscle tissue and 20 other tissues |
Genevisiblei | A4GTP4, RN |
Family and domain databases
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR000418, Ets_dom IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF00178, Ets, 1 hit |
PRINTSi | PR00454, ETSDOMAIN |
SMARTi | View protein in SMART SM00413, ETS, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit |
PROSITEi | View protein in PROSITE PS00345, ETS_DOMAIN_1, 1 hit PS00346, ETS_DOMAIN_2, 1 hit PS50061, ETS_DOMAIN_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ELK1_RAT | |
Accessioni | A4GTP4Primary (citable) accession number: A4GTP4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 14, 2015 |
Last sequence update: | April 17, 2007 | |
Last modified: | April 7, 2021 | |
This is version 115 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families