UniProtKB - A4FUD3 (U5S1_BOVIN)
116 kDa U5 small nuclear ribonucleoprotein component
EFTUD2
Functioni
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 136 – 143 | GTPSequence analysis | 8 | |
Nucleotide bindingi | 204 – 208 | GTPSequence analysis | 5 | |
Nucleotide bindingi | 258 – 261 | GTPSequence analysis | 4 |
GO - Molecular functioni
- GTPase activity Source: GO_Central
- GTP binding Source: UniProtKB-KW
- U5 snRNA binding Source: GO_Central
GO - Biological processi
- mRNA splicing, via spliceosome Source: UniProtKB
Keywordsi
Biological process | mRNA processing, mRNA splicing |
Ligand | GTP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: 116 kDa U5 small nuclear ribonucleoprotein componentAlternative name(s): Elongation factor Tu GTP-binding domain protein 2 U5 snRNP-specific protein, 116 kDa Short name: U5-116 kDa |
Gene namesi | Name:EFTUD2 Synonyms:SNRP116 |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Subcellular locationi
Nucleus
- Nucleus By similarity
Nucleus
- nucleus Source: UniProtKB
- U2-type catalytic step 2 spliceosome Source: UniProtKB
- U2-type precatalytic spliceosome Source: UniProtKB
- U4/U6 x U5 tri-snRNP complex Source: GO_Central
Other locations
- ribonucleoprotein complex Source: GO_Central
Keywords - Cellular componenti
Nucleus, SpliceosomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000315996 | 1 – 972 | 116 kDa U5 small nuclear ribonucleoprotein componentAdd BLAST | 972 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 19 | PhosphoserineBy similarity | 1 | |
Cross-linki | 64 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity | ||
Cross-linki | 64 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Modified residuei | 86 | PhosphothreonineBy similarity | 1 |
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
PaxDbi | A4FUD3 |
PRIDEi | A4FUD3 |
Interactioni
Subunit structurei
Component of the U5 snRNP and the U4/U6-U5 tri-snRNP complex, a building block of the spliceosome (By similarity). The U4/U6-U5 tri-snRNP complex is composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39 (By similarity).
Component of the pre-catalytic, catalytic and post-catalytic spliceosome complexes (By similarity).
Interacts with ERBB4 and PRPF8 (By similarity).
Interacts with PIH1D1 (By similarity).
Interacts with RPAP3 and URI1 in a ZNHIT2-dependent manner (By similarity).
Interacts with NRDE2 (By similarity).
By similarityProtein-protein interaction databases
STRINGi | 9913.ENSBTAP00000017986 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 127 – 409 | tr-type GPROSITE-ProRule annotationAdd BLAST | 283 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 2 – 51 | Asp-richAdd BLAST | 50 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0468, Eukaryota |
InParanoidi | A4FUD3 |
OrthoDBi | 140796at2759 |
Family and domain databases
CDDi | cd04098, eEF2_C_snRNP, 1 hit cd04167, Snu114p, 1 hit |
Gene3Di | 3.30.230.10, 1 hit |
InterProi | View protein in InterPro IPR041095, EFG_II IPR035647, EFG_III/V IPR000640, EFG_V-like IPR004161, EFTu-like_2 IPR031950, EFTUD2_N IPR027417, P-loop_NTPase IPR020568, Ribosomal_S5_D2-typ_fold IPR014721, Ribosomal_S5_D2-typ_fold_subgr IPR005225, Small_GTP-bd_dom IPR044121, Snu114_GTP-bd IPR000795, T_Tr_GTP-bd_dom IPR009000, Transl_B-barrel_sf IPR005517, Transl_elong_EFG/EF2_IV IPR035655, U5-116kDa_C |
Pfami | View protein in Pfam PF00679, EFG_C, 1 hit PF14492, EFG_III, 1 hit PF03764, EFG_IV, 1 hit PF16004, EFTUD2, 1 hit PF00009, GTP_EFTU, 1 hit PF03144, GTP_EFTU_D2, 1 hit |
PRINTSi | PR00315, ELONGATNFCT |
SMARTi | View protein in SMART SM00838, EFG_C, 1 hit SM00889, EFG_IV, 1 hit |
SUPFAMi | SSF50447, SSF50447, 1 hit SSF52540, SSF52540, 1 hit SSF54211, SSF54211, 1 hit SSF54980, SSF54980, 2 hits |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51722, G_TR_2, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MDTDLYDEFG NYIGPELDSD EDDDELGRET KDLDEVDEDE DDDDVGDHDE
60 70 80 90 100
DHPGMEVVLH EDKKYYPTAE EVYGPEVETI VQEEDTQPLT EPIIKPVKTK
110 120 130 140 150
KFTLMEQTLP VTVYEMDSLA DLMDNSELIR NVTLCGHLHH GKTCFVDCLI
160 170 180 190 200
EQTHPEIRKR YDQDLCYTDI LFTEQERGVG IKSTPVTVVL PDTKGKSYLF
210 220 230 240 250
NIMDTPGHVN FSDEVTAGLR ISDGVVLFID AAEGVMLNTE RLIKHAVQER
260 270 280 290 300
LAVTVCINKI DRLILELKLP PTDAYYKLRH IVDEVNGLIS MYSTDENLIL
310 320 330 340 350
SPLLGNVCFS SSQYSICFTL GSFAKIYADT FGDINYQEFA KRLWGDIYFN
360 370 380 390 400
PKTRKFTKKA PTSSSQRSFV EFILEPLYKI LAQVVGDVDT SLPRTLDELG
410 420 430 440 450
IHLTKEELKL NIRPLLRLVC KKFFGEFTGF VDMCVQHIPS PKVGAKPKIE
460 470 480 490 500
HTYTGGVDSD LGEAMSDCDP DGPLMCHTTK MYSTDDGVQF HAFGRVLSGT
510 520 530 540 550
IHAGQPVKVL GENYTLEDEE DSQICTVGRL WISVARYHIE VNRVPAGNWV
560 570 580 590 600
LIEGVDQPIV KTATITEPRG NEEAQIFRPL KFNTTSVIKI AVEPVNPSEL
610 620 630 640 650
PKMLDGLRKV NKSYPSLTTK VEESGEHVIL GTGELYLDCV MHDLRKMYSE
660 670 680 690 700
IDIKVADPVV TFCETVVETS SLKCFAETPN KKNKITMIAE PLEKGLAEDI
710 720 730 740 750
ENEVVQITWN RKKLGEFFQT KYDWDLLAAR SIWAFGPDAT GPNILVDDTL
760 770 780 790 800
PSEVDKALLG SVKDSIVQGF QWGTREGPLC DELIRNVKFK ILDAVVAQEP
810 820 830 840 850
LHRGGGQIIP TARRVVYSAF LMATPRLMEP YYFVEVQAPA DCVSAVYTVL
860 870 880 890 900
ARRRGHVTQD APIPGSPLYT IKAFIPAIDS FGFETDLRTH TQGQAFSLSV
910 920 930 940 950
FHHWQIVPGD PLDKSIVIRP LEPQPAPHLA REFMIKTRRR KGLSEDVSIS
960 970
KFFDDPMLLE LAKQDVVLNY PM
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC114717 mRNA Translation: AAI14718.1 |
RefSeqi | NP_001076865.1, NM_001083396.1 |
Genome annotation databases
GeneIDi | 509425 |
KEGGi | bta:509425 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC114717 mRNA Translation: AAI14718.1 |
RefSeqi | NP_001076865.1, NM_001083396.1 |
3D structure databases
SMRi | A4FUD3 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000017986 |
Proteomic databases
PaxDbi | A4FUD3 |
PRIDEi | A4FUD3 |
Genome annotation databases
GeneIDi | 509425 |
KEGGi | bta:509425 |
Organism-specific databases
CTDi | 9343 |
Phylogenomic databases
eggNOGi | KOG0468, Eukaryota |
InParanoidi | A4FUD3 |
OrthoDBi | 140796at2759 |
Family and domain databases
CDDi | cd04098, eEF2_C_snRNP, 1 hit cd04167, Snu114p, 1 hit |
Gene3Di | 3.30.230.10, 1 hit |
InterProi | View protein in InterPro IPR041095, EFG_II IPR035647, EFG_III/V IPR000640, EFG_V-like IPR004161, EFTu-like_2 IPR031950, EFTUD2_N IPR027417, P-loop_NTPase IPR020568, Ribosomal_S5_D2-typ_fold IPR014721, Ribosomal_S5_D2-typ_fold_subgr IPR005225, Small_GTP-bd_dom IPR044121, Snu114_GTP-bd IPR000795, T_Tr_GTP-bd_dom IPR009000, Transl_B-barrel_sf IPR005517, Transl_elong_EFG/EF2_IV IPR035655, U5-116kDa_C |
Pfami | View protein in Pfam PF00679, EFG_C, 1 hit PF14492, EFG_III, 1 hit PF03764, EFG_IV, 1 hit PF16004, EFTUD2, 1 hit PF00009, GTP_EFTU, 1 hit PF03144, GTP_EFTU_D2, 1 hit |
PRINTSi | PR00315, ELONGATNFCT |
SMARTi | View protein in SMART SM00838, EFG_C, 1 hit SM00889, EFG_IV, 1 hit |
SUPFAMi | SSF50447, SSF50447, 1 hit SSF52540, SSF52540, 1 hit SSF54211, SSF54211, 1 hit SSF54980, SSF54980, 2 hits |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51722, G_TR_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | U5S1_BOVIN | |
Accessioni | A4FUD3Primary (citable) accession number: A4FUD3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 15, 2008 |
Last sequence update: | April 17, 2007 | |
Last modified: | April 7, 2021 | |
This is version 87 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families