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Protein

Aspartate--tRNA ligase

Gene

aspS

Organism
Shewanella loihica (strain ATCC BAA-1088 / PV-4)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of L-aspartate to tRNA(Asp) in a two-step reaction: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp).UniRule annotation

Catalytic activityi

ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei173AspartateUniRule annotation1
Binding sitei219AspartateUniRule annotation1
Binding sitei228ATPUniRule annotation1
Binding sitei449AspartateUniRule annotation1
Binding sitei483ATPUniRule annotation1
Binding sitei490AspartateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi219 – 221ATPUniRule annotation3
Nucleotide bindingi535 – 538ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSLOI323850:G1G8D-2202-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartate--tRNA ligaseUniRule annotation (EC:6.1.1.12UniRule annotation)
Alternative name(s):
Aspartyl-tRNA synthetaseUniRule annotation
Short name:
AspRSUniRule annotation
Gene namesi
Name:aspSUniRule annotation
Ordered Locus Names:Shew_2079
OrganismiShewanella loihica (strain ATCC BAA-1088 / PV-4)
Taxonomic identifieri323850 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
Proteomesi
  • UP000001558 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000067571 – 592Aspartate--tRNA ligaseAdd BLAST592

Proteomic databases

PRIDEiA3QEP7

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi323850.Shew_2079

Structurei

3D structure databases

ProteinModelPortaliA3QEP7
SMRiA3QEP7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni197 – 200AspartateUniRule annotation4

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C9M Bacteria
COG0173 LUCA
HOGENOMiHOG000275160
KOiK01876
OMAiYQLDVEM
OrthoDBiPOG091H021G

Family and domain databases

Gene3Di3.30.1360.30, 1 hit
HAMAPiMF_00044 Asp_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR004364 aa-tRNA-synt_II
IPR006195 aa-tRNA-synth_II
IPR004524 Asp-tRNA-ligase_1
IPR002312 Asp/Asn-tRNA-synth_IIb
IPR004115 GAD-like_sf
IPR029351 GAD_dom
IPR012340 NA-bd_OB-fold
IPR004365 NA-bd_OB_tRNA
PANTHERiPTHR22594:SF5 PTHR22594:SF5, 1 hit
PfamiView protein in Pfam
PF02938 GAD, 1 hit
PF00152 tRNA-synt_2, 1 hit
PF01336 tRNA_anti-codon, 1 hit
PRINTSiPR01042 TRNASYNTHASP
SUPFAMiSSF50249 SSF50249, 1 hit
SSF55261 SSF55261, 1 hit
TIGRFAMsiTIGR00459 aspS_bact, 1 hit
PROSITEiView protein in PROSITE
PS50862 AA_TRNA_LIGASE_II, 1 hit

Sequencei

Sequence statusi: Complete.

A3QEP7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRSHYCGDVN RSHVGEEVTL VGWVNRSRDL GGVVFLDLRD REGVIQVVYD
60 70 80 90 100
PDLPEVFDVA STLRSEFCVQ VKGLVRARPD SQINDQMRTG EIEVLGKALT
110 120 130 140 150
ILNSAPALPI NMDKNQHNTE EQRLKYRYLD LRRPEMAERI IFRSKVTSAV
160 170 180 190 200
RRFLDGNGFL DIETPILTKA TPEGARDYLV PSRTYKGQFF ALPQSPQLFK
210 220 230 240 250
QLLMMSGFDR YYQIVKCFRD EDLRADRQPE FTQIDIETSF MTSAQVMDKT
260 270 280 290 300
EEMVRGLFKE LLNVDLGEFP KMTFAEAMRR YGSDKPDLRN PLELVDVADL
310 320 330 340 350
VKDVDFKVFQ EPANDSEGRV AVLCVPGGAS LSRKQLDEYG KYVNIYGAKG
360 370 380 390 400
LAWMKVNELE NGLEGIQSPV LKFLSEEVVK GILERTGAAN GDLILFGADK
410 420 430 440 450
ANIVAEAMGA LRLKVGEDFD LLQGDWKPLW VVDFPMFERT SDGGLHAMHH
460 470 480 490 500
PFTAPSGITP AELEADPTAA ISDAYDMVLN GCELGGGSVR IYDAEMQSAV
510 520 530 540 550
FRILGINDEE AQEKFGFLLE ALKYGTPPHA GLAFGLDRMV MLMTGASSIR
560 570 580 590
DVMAFPKTTT AACPLTNAPG FANPVQLEEL GVSVVEAKKE QE
Length:592
Mass (Da):65,939
Last modified:April 17, 2007 - v1
Checksum:iD522065BAA27EE7D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000606 Genomic DNA Translation: ABO23945.1
RefSeqiWP_011865877.1, NC_009092.1

Genome annotation databases

EnsemblBacteriaiABO23945; ABO23945; Shew_2079
KEGGislo:Shew_2079

Similar proteinsi

Entry informationi

Entry nameiSYD_SHELP
AccessioniPrimary (citable) accession number: A3QEP7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: April 17, 2007
Last modified: July 18, 2018
This is version 85 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

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