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Protein

UDP-2,3-diacylglucosamine hydrolase

Gene

lpxH

Organism
Burkholderia pseudomallei (strain 1106a)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Hydrolyzes the pyrophosphate bond of UDP-2,3-diacylglucosamine to yield 2,3-diacylglucosamine 1-phosphate (lipid X) and UMP by catalyzing the attack of water at the alpha-P atom. Involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.UniRule annotationSAAS annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Catalytic activityi

UDP-2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosamine + H2O = 2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate + UMP.UniRule annotationSAAS annotation

Cofactori

Mn2+UniRule annotationNote: Binds 2 Mn2+ ions per subunit in a binuclear metal center.UniRule annotation

Pathwayi: lipid IV(A) biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine.UniRule annotationSAAS annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA)
  2. UDP-3-O-acyl-N-acetylglucosamine deacetylase (wcbS), UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC)
  3. no protein annotated in this organism
  4. UDP-2,3-diacylglucosamine hydrolase (lpxH)
  5. Lipid-A-disaccharide synthase (lpxB)
  6. Tetraacyldisaccharide 4'-kinase (lpxK)
This subpathway is part of the pathway lipid IV(A) biosynthesis, which is itself part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine, the pathway lipid IV(A) biosynthesis and in Glycolipid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi23Manganese 1UniRule annotation1
Metal bindingi25Manganese 1; via tele nitrogenUniRule annotation1
Metal bindingi56Manganese 1UniRule annotation1
Metal bindingi56Manganese 2UniRule annotation1
Metal bindingi96Manganese 2UniRule annotation1
Metal bindingi131Manganese 2; via tele nitrogenUniRule annotation1
Binding sitei139SubstrateUniRule annotation1
Binding sitei177SubstrateUniRule annotation1
Metal bindingi212Manganese 2; via pros nitrogenUniRule annotation1
Binding sitei212Substrate; via tele nitrogenUniRule annotation1
Metal bindingi214Manganese 1; via tele nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolaseUniRule annotationSAAS annotationImported
Biological processLipid A biosynthesisUniRule annotationSAAS annotation, Lipid biosynthesis, Lipid metabolism
LigandManganeseUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00359; UER00480

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-2,3-diacylglucosamine hydrolaseUniRule annotationSAAS annotation (EC:3.6.1.54UniRule annotationSAAS annotation)
Alternative name(s):
UDP-2,3-diacylglucosamine diphosphataseUniRule annotation
Gene namesi
Name:lpxHUniRule annotationImported
Ordered Locus Names:BURPS1106A_2600Imported
OrganismiBurkholderia pseudomallei (strain 1106a)Imported
Taxonomic identifieri357348 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiapseudomallei group
Proteomesi
  • UP000006738 Componenti: Chromosome I

Subcellular locationi

  • Cell inner membrane UniRule annotationSAAS annotation; Peripheral membrane protein UniRule annotationSAAS annotation; Cytoplasmic side UniRule annotationSAAS annotation
  • Cytoplasm SAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membraneUniRule annotationSAAS annotation, Cell membrane, Membrane

Structurei

3D structure databases

ProteinModelPortaliA3NWY2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini17 – 216MetallophosInterPro annotationAdd BLAST200

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni96 – 97Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the LpxH family.UniRule annotationSAAS annotation

Phylogenomic databases

HOGENOMiHOG000261930
KOiK03269
OMAiFDFWFEY

Family and domain databases

HAMAPiMF_00575 LpxH, 1 hit
InterProiView protein in InterPro
IPR004843 Calcineurin-like_PHP_ApaH
IPR010138 UDP-diacylglucosamine_Hdrlase
PANTHERiPTHR34990:SF1 PTHR34990:SF1, 1 hit
PfamiView protein in Pfam
PF00149 Metallophos, 1 hit
TIGRFAMsiTIGR01854 lipid_A_lpxH, 1 hit

Sequencei

Sequence statusi: Complete.

A3NWY2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFAGVPGERG LAHAARPFLF VSDLHLSDAI PHTVAAFERF VRVTADSADS
60 70 80 90 100
VFILGDLFEY WIGDDILEHD PFAARMAALL HTFSERGIAL YVMHGNRDFL
110 120 130 140 150
LGKRFMKAAG ALLIPDPSVI VAFGNRIVLA HGDAQCTADR GYQWFRRFAR
160 170 180 190 200
NGVAQRLFLA WPFAWRRALA QRMRASSETG RMRPASPRYD VTRKGVAALL
210 220 230 240 250
RRSGARTIIH GHTHKPARHV EADGVRWVLP DWELDHGLRR GGYLRVDAEG

IRALPLD
Length:257
Mass (Da):28,816
Last modified:April 3, 2007 - v1
Checksum:i4A95922C5FE00957
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000572 Genomic DNA Translation: ABN90513.1

Genome annotation databases

EnsemblBacteriaiABN90513; ABN90513; BURPS1106A_2600
KEGGibpl:BURPS1106A_2600

Similar proteinsi

Entry informationi

Entry nameiA3NWY2_BURP0
AccessioniPrimary (citable) accession number: A3NWY2
Entry historyiIntegrated into UniProtKB/TrEMBL: April 3, 2007
Last sequence update: April 3, 2007
Last modified: March 28, 2018
This is version 82 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

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