UniProtKB - A3MQB6 (ISPE_BURM7)
Protein
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Gene
ispE
Organism
Burkholderia mallei (strain NCTC 10247)
Status
Functioni
Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol.UniRule annotation
Catalytic activityi
- 4-CDP-2-C-methyl-D-erythritol + ATP = 4-CDP-2-C-methyl-D-erythritol 2-phosphate + ADP + H+UniRule annotationEC:2.7.1.148UniRule annotation
: isopentenyl diphosphate biosynthesis via DXP pathway Pathwayi
This protein is involved in step 3 of the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate.UniRule annotationProteins known to be involved in the 6 steps of the subpathway in this organism are:
- 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr)
- 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD)
- 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE)
- 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF)
- 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG)
- 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH-1), 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH-2)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate, the pathway isopentenyl diphosphate biosynthesis via DXP pathway and in Isoprenoid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 16 | UniRule annotation | 1 | |
Active sitei | 141 | UniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 99 – 109 | ATPUniRule annotationAdd BLAST | 11 |
GO - Molecular functioni
- 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Source: UniProtKB-UniRule
- ATP binding Source: UniProtKB-UniRule
GO - Biological processi
- isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway Source: UniProtKB-UniPathway
- terpenoid biosynthetic process Source: UniProtKB-UniRule
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Isoprene biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
UniPathwayi | UPA00056;UER00094 |
Names & Taxonomyi
Protein namesi | Recommended name: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinaseUniRule annotation (EC:2.7.1.148UniRule annotation)Short name: CMKUniRule annotation Alternative name(s): 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinaseUniRule annotation |
Gene namesi | Name:ispEUniRule annotation Ordered Locus Names:BMA10247_2932 |
Organismi | Burkholderia mallei (strain NCTC 10247) |
Taxonomic identifieri | 320389 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_1000007820 | 1 – 293 | 4-diphosphocytidyl-2-C-methyl-D-erythritol kinaseAdd BLAST | 293 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
OMAi | RWPSPAK |
Family and domain databases
Gene3Di | 3.30.230.10, 1 hit 3.30.70.890, 1 hit |
HAMAPi | MF_00061, IspE, 1 hit |
InterProi | View protein in InterPro IPR013750, GHMP_kinase_C_dom IPR036554, GHMP_kinase_C_sf IPR006204, GHMP_kinase_N_dom IPR004424, IspE IPR020568, Ribosomal_S5_D2-typ_fold IPR014721, Ribosomal_S5_D2-typ_fold_subgr |
PANTHERi | PTHR43527:SF2, PTHR43527:SF2, 1 hit |
Pfami | View protein in Pfam PF08544, GHMP_kinases_C, 1 hit PF00288, GHMP_kinases_N, 1 hit |
PIRSFi | PIRSF010376, IspE, 1 hit |
SUPFAMi | SSF54211, SSF54211, 1 hit SSF55060, SSF55060, 1 hit |
TIGRFAMsi | TIGR00154, ispE, 1 hit |
i Sequence
Sequence statusi: Complete.
A3MQB6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTDTTRSLRD CLAPAKLNLF LHITGRRPDG YHALQSVFQL LDWGDRLHFT
60 70 80 90 100
LRDDGKVSRV TDVPGVPEES DLVVRAASLL KAHAGATLGV DIEIDKRLPM
110 120 130 140 150
GAGLGGGSSD AATTLLALNR LWRLDLPRTT LQSLAVKLGA DVPFFVFGKN
160 170 180 190 200
AFAEGIGEAL QAVELPARWF LVVTPRVHVP TAAIFSEKSL TRDSKPITIT
210 220 230 240 250
DFLAQRGIDA GWPDSFGRND MQPVVTSKYA EVAKVVEWFY NLTPARMTGS
260 270 280 290
GASVFAAFKS KADAEAAQAK LPAGWNSAVA ESMSEHPLFA FAS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000548 Genomic DNA Translation: ABO05693.1 |
RefSeqi | WP_004195241.1, NZ_CP007802.1 |
Genome annotation databases
GeneIDi | 56593810 |
KEGGi | bmaz:BM44_414 bmn:BMA10247_2932 |
PATRICi | fig|320389.8.peg.456 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000548 Genomic DNA Translation: ABO05693.1 |
RefSeqi | WP_004195241.1, NZ_CP007802.1 |
3D structure databases
SMRi | A3MQB6 |
ModBasei | Search... |
Genome annotation databases
GeneIDi | 56593810 |
KEGGi | bmaz:BM44_414 bmn:BMA10247_2932 |
PATRICi | fig|320389.8.peg.456 |
Phylogenomic databases
OMAi | RWPSPAK |
Enzyme and pathway databases
UniPathwayi | UPA00056;UER00094 |
Family and domain databases
Gene3Di | 3.30.230.10, 1 hit 3.30.70.890, 1 hit |
HAMAPi | MF_00061, IspE, 1 hit |
InterProi | View protein in InterPro IPR013750, GHMP_kinase_C_dom IPR036554, GHMP_kinase_C_sf IPR006204, GHMP_kinase_N_dom IPR004424, IspE IPR020568, Ribosomal_S5_D2-typ_fold IPR014721, Ribosomal_S5_D2-typ_fold_subgr |
PANTHERi | PTHR43527:SF2, PTHR43527:SF2, 1 hit |
Pfami | View protein in Pfam PF08544, GHMP_kinases_C, 1 hit PF00288, GHMP_kinases_N, 1 hit |
PIRSFi | PIRSF010376, IspE, 1 hit |
SUPFAMi | SSF54211, SSF54211, 1 hit SSF55060, SSF55060, 1 hit |
TIGRFAMsi | TIGR00154, ispE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ISPE_BURM7 | |
Accessioni | A3MQB6Primary (citable) accession number: A3MQB6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 15, 2008 |
Last sequence update: | April 3, 2007 | |
Last modified: | April 7, 2021 | |
This is version 71 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Documents
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families