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Protein

Vacuolar protein sorting-associated protein 27

Gene

VPS27

Organism
Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545) (Yeast) (Pichia stipitis)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Component of the ESCRT-0 complex which is the sorting receptor for ubiquitinated cargo proteins at the multivesicular body (MVB) and recruits ESCRT-I to the MVB outer membrane.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri187 – 247FYVE-typePROSITE-ProRule annotationAdd BLAST61

GO - Molecular functioni

GO - Biological processi

Keywordsi

LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein sorting-associated protein 27
Gene namesi
Name:VPS27
ORF Names:PICST_36619
OrganismiScheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545) (Yeast) (Pichia stipitis)
Taxonomic identifieri322104 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeScheffersomyces
Proteomesi
  • UP000002258 Componenti: Chromosome 6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002925221 – 732Vacuolar protein sorting-associated protein 27Add BLAST732

Interactioni

Subunit structurei

Component of the ESCRT-0 complex composed of HSE1 and VPS27.

Protein-protein interaction databases

STRINGi322104.XP_001385783.2

Structurei

3D structure databases

ProteinModelPortaliA3LX75
SMRiA3LX75
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 166VHSPROSITE-ProRule annotationAdd BLAST144
Domaini271 – 290UIM 1PROSITE-ProRule annotationAdd BLAST20
Domaini313 – 332UIM 2PROSITE-ProRule annotationAdd BLAST20

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi469 – 562Gln-richAdd BLAST94

Domaini

The FYVE domain is involved in the binding to phosphatidylinositol 3-phosphate (PtdIns3P) which is required for the association to endosomal membranes.
Both IUM domains are necessary for efficient binding to ubiquitin.By similarity

Sequence similaritiesi

Belongs to the VPS27 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri187 – 247FYVE-typePROSITE-ProRule annotationAdd BLAST61

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1818 Eukaryota
ENOG410XNRF LUCA
HOGENOMiHOG000048363
InParanoidiA3LX75
KOiK12182
OMAiYMYPAGA
OrthoDBiEOG092C214M

Family and domain databases

Gene3Di1.25.40.90, 1 hit
3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR008942 ENTH_VHS
IPR003903 UIM_dom
IPR002014 VHS_dom
IPR000306 Znf_FYVE
IPR017455 Znf_FYVE-rel
IPR011011 Znf_FYVE_PHD
IPR013083 Znf_RING/FYVE/PHD
PfamiView protein in Pfam
PF01363 FYVE, 1 hit
PF02809 UIM, 2 hits
PF00790 VHS, 1 hit
SMARTiView protein in SMART
SM00064 FYVE, 1 hit
SM00726 UIM, 2 hits
SM00288 VHS, 1 hit
SUPFAMiSSF48464 SSF48464, 1 hit
SSF57903 SSF57903, 1 hit
PROSITEiView protein in PROSITE
PS50330 UIM, 2 hits
PS50179 VHS, 1 hit
PS50178 ZF_FYVE, 1 hit

Sequencei

Sequence statusi: Complete.

A3LX75-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSWFGSSSDS TIELDNKIQE ATSESIPNGE LDLPLALEVT DLIRSKSLPP
60 70 80 90 100
IQCMRSLKKR LGMTYSNPNL LSSTLKLVDL CIKNCGSHFL NEIASKEFMD
110 120 130 140 150
YLVDFIFKVH YDTKNYQVRN SEAKMNVGEL ILSLIKEWSI LFSNSSDLSY
160 170 180 190 200
VTRCFERLES EAYNFPDFAE TSALNSKFVD TEVPPDWVDD DKCMICYDKF
210 220 230 240 250
SMINRKHHCR ACGGVFCQTH SSNFIPLVSL GISKPVRACD NCLAKQKSKN
260 270 280 290 300
KPSQHNSSSH SRGTSRVQED DEDEMLRKAI ELSLQDTQIP VSAPVARDAP
310 320 330 340 350
TNSSQTKTIT DEDDDEDLKA AIAASMKDYQ DQERLREEQQ KQWQQEQEEK
360 370 380 390 400
HHQEEQSDFY NYSIPKPSNY SQQLPYVQSV ASLTEEEEAD INKFITLMYQ
410 420 430 440 450
VKNDQNISYA RLHDEKLTDL HTKVCPLRSK VTKNLIYTVE RQKAFTELNN
460 470 480 490 500
KIAAITRLYE EHLDSKLKQT YGYQQSVPPV QLPEINDYMR GPQSNYEQSI
510 520 530 540 550
PAQGTGYQQQ AGYVHPETTG YPSYPGGQQP IPQQPIAQQP SGSRPSAQQA
560 570 580 590 600
QKAPEQKHEQ QQTSVPTFAY PPQESYPPEG EDDEEQSNFE LPPLPNQAQN
610 620 630 640 650
DFSYPPTQSY DSPSEPMYPN DSTDQQYNVY PPNGAGSEES KDEYSSGELQ
660 670 680 690 700
PVQPTREHVL RTRSSELPPH AVEQASARFP PIDTVEEEYQ NSNNSSVPYP
710 720 730
DVSFPIAPTQ NLQPQQEPPK KFVPEPEPLI DI
Length:732
Mass (Da):83,145
Last modified:July 24, 2007 - v2
Checksum:i15151640367D4D98
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000500 Genomic DNA Translation: ABN67754.2
RefSeqiXP_001385783.2, XM_001385746.1

Genome annotation databases

EnsemblFungiiABN67754; ABN67754; PICST_36619
GeneIDi4839973
KEGGipic:PICST_36619

Entry informationi

Entry nameiVPS27_PICST
AccessioniPrimary (citable) accession number: A3LX75
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: July 24, 2007
Last modified: March 28, 2018
This is version 66 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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