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UniProtKB - A3KGV1 (ODFP2_MOUSE)

Protein

Outer dense fiber protein 2

Gene

Odf2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Seems to be a major component of sperm tail outer dense fibers (ODF). ODFs are filamentous structures located on the outside of the axoneme in the midpiece and principal piece of the mammalian sperm tail and may help to maintain the passive elastic structures and elastic recoil of the sperm tail. May have a modulating influence on sperm motility. Functions as a general scaffold protein that is specifically localized at the distal/subdistal appendages of mother centrioles. Component of the centrosome matrix required for the localization of PLK1 and NIN to the centrosomes. Required for the formation and/or maintenance of normal CETN1 assembly (By similarity).By similarity1 Publication

GO - Molecular functioni

  • Rab GTPase binding Source: MGI
  • structural constituent of cytoskeleton Source: MGI
View the complete GO annotation on QuickGO ...

GO - Biological processi

View the complete GO annotation on QuickGO ...

Keywordsi

Molecular functionDevelopmental protein
Biological processDifferentiation, Spermatogenesis

Enzyme and pathway databases

ReactomeiR-MMU-2565942 Regulation of PLK1 Activity at G2/M Transition
R-MMU-380259 Loss of Nlp from mitotic centrosomes
R-MMU-380270 Recruitment of mitotic centrosome proteins and complexes
R-MMU-380320 Recruitment of NuMA to mitotic centrosomes
R-MMU-5620912 Anchoring of the basal body to the plasma membrane
R-MMU-8854518 AURKA Activation by TPX2

Names & Taxonomyi

Protein namesi
Recommended name:
Outer dense fiber protein 2
Alternative name(s):
84 kDa outer dense fiber protein
Cenexin
Outer dense fiber of sperm tails protein 2
Gene namesi
Name:Odf2
Synonyms:Odf84
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1098824 Odf2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cilium, Cytoplasm, Cytoskeleton, Flagellum, Microtubule

Pathology & Biotechi

Disruption phenotypei

Null mutations in F9 embryonic carcinoma cells eliminated distal/subdistal appendages and prevented primary cilium formation. Loss of ODF2 also disrupted two mother centriole-specific NIN dots, while leaving one dot on the proximal end of mother and daughter centrioles.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002994581 – 830Outer dense fiber protein 2Add BLAST830

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei73PhosphoserineBy similarity1
Modified residuei74PhosphoserineBy similarity1
Modified residuei92PhosphothreonineBy similarity1
Modified residuei95Phosphoserine; by TSSK41 Publication1
Modified residuei106PhosphoserineBy similarity1
Modified residuei109PhosphoserineBy similarity1
Modified residuei110PhosphothreonineBy similarity1
Modified residuei115PhosphoserineBy similarity1
Modified residuei129PhosphoserineBy similarity1
Cross-linki138Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei139PhosphoserineBy similarity1
Modified residuei231PhosphothreonineBy similarity1
Modified residuei261PhosphoserineBy similarity1
Modified residuei632PhosphoserineBy similarity1

Post-translational modificationi

Tyrosine phosphorylated (By similarity). Phosphorylated on Ser-95 by TSSK4 (PubMed:26961893).By similarity1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiA3KGV1
PaxDbiA3KGV1
PeptideAtlasiA3KGV1
PRIDEiA3KGV1

2D gel databases

REPRODUCTION-2DPAGEiA3KGV1
IPI00130958

PTM databases

iPTMnetiA3KGV1
PhosphoSitePlusiA3KGV1

Expressioni

Tissue specificityi

Testis-specific (at protein level) (PubMed:9045620, PubMed:27682589). Expressed in spermatids at tubular stage V of the spermatogenic cycle (PubMed:9740324). Highly expressed in the cytoplasm of elongating spermatids (tubular stages X/XI) (PubMed:9740324). In step 14/15 spermatids of tubular stage III/IV low expression detected (PubMed:9740324). No expression detected in other testicular cells as well as the early round of spermatids (PubMed:9740324).3 Publications

Gene expression databases

BgeeiENSMUSG00000026790 Expressed in 292 organ(s), highest expression level in testis
CleanExiMM_ODF2
ExpressionAtlasiA3KGV1 baseline and differential
GenevisibleiA3KGV1 MM

Interactioni

Subunit structurei

Self-associates. Associates with microtubules and forms a fibrillar structure partially linked to the microtubule network. Interacts via its C-terminus with PLK1 (By similarity). Interacts with ODF1 (PubMed:9045620). Interacts with MARK4; the interaction is required for localization of ODF2 to centrioles (By similarity). Interacts with TSSK4 (PubMed:25361759, PubMed:26961893).By similarity3 Publications

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridi201898, 3 interactors
IntActiA3KGV1, 3 interactors
STRINGi10090.ENSMUSP00000109388

Structurei

3D structure databases

ProteinModelPortaliA3KGV1
SMRiA3KGV1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili144 – 217Sequence analysisAdd BLAST74
Coiled coili245 – 423Sequence analysisAdd BLAST179
Coiled coili461 – 798Sequence analysisAdd BLAST338

Sequence similaritiesi

Belongs to the ODF2 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IE8V Eukaryota
ENOG4111XWH LUCA
GeneTreeiENSGT00530000063497
HOVERGENiHBG052808
InParanoidiA3KGV1
KOiK16479
OMAiNQAHIEV
OrthoDBiEOG091G13KV
PhylomeDBiA3KGV1
TreeFamiTF328605

Family and domain databases

InterProiView protein in InterPro
IPR026099 Odf2-rel
PANTHERiPTHR23162 PTHR23162, 1 hit

Sequences (7+)i

Sequence statusi: Complete.

This entry describes 7 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 7 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: A3KGV1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSASSSGGSP RFPSCGKNGV TSLTQKKVLR TPCGAPSVTV TKSHKRGMKG 
        60         70         80         90        100
DTVNVRRSVR VKTKVPWMPP GKSSARHVGC KWENPPHCLE ITPPSSEKLV 
       110        120        130        140        150
SVMRLSDLST EDDDSGHCKM NRYDKKIDSL MNAVGCLKSE VKMQKGERQM 
       160        170        180        190        200
AKRFLEERKE ELEEVAHELA ETEHENTVLR HNIERIKEEK DFTMLQKKHL 
       210        220        230        240        250
QQEKECLMSK LVEAEMDGAA AAKQVMALKD TIGKLKTEKQ MTCTDINTLT 
       260        270        280        290        300
RQKELLLQKL STFEETNRTL RDLLREQHCK EDSERLMEQQ GTLLKRLAEA 
       310        320        330        340        350
DSEKARLLLL LQDKDKEVEE LLQEIQCEKA QAKTASELSK SMESMRGHLQ 
       360        370        380        390        400
AQLRCKEAEN SRLCMQIKNL ERSGNQHKAE VEAIMEQLKE LKQKGDRDKE 
       410        420        430        440        450
TLKKAIRAQK ERAEKSEEYA EQLHVQLADK DLYVAEALST LESWRSRYNQ 
       460        470        480        490        500
VVKDKGDLEL EIIVLNDRVT DLVNQQQSLE EKMREDRDSL VERLHRQTAE 
       510        520        530        540        550
YSAFKLENER LKASFAPMED KLNQAHLEVQ QLKASVKNYE GMIDNYKSQV 
       560        570        580        590        600
MKTRLEADEV AAQLERCDKE NKMLKDEMNK EIEAARRQFQ SQLADLQQLP 
       610        620        630        640        650
DILKITEAKL AECQDQLQGY ERKNIDLTAI ISDLRSRIEH QGDKLEMARE 
       660        670        680        690        700
KHQASQKENK QLSQKVDELE RKLEATSAQN VEFLQVIAKR EEAIHQAQLR 
       710        720        730        740        750
LEEKTRECGS LARQLESAIE DARRQVEQTK EQALSKERAA QSKILDLETQ 
       760        770        780        790        800
LSRTKTELGQ LRRTRDDADR RYQSRLQDLK DRLEQSESTN RSMQNYVQFL 
       810        820        830
KASYANVFGD APYTSSYLTS SPIRSRSPPA
Note: Gene prediction based on EST data.
Length:830
Mass (Da):95,541
Last modified:April 3, 2007 - v1
Checksum:iC840B47EC10EF82A
GO
Isoform 2 (identifier: A3KGV1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-41: MSASSSGGSP...PCGAPSVTVT → MKDRSSTPPL...LPKPSAASSQ
     65-83: Missing.

Note: Gene prediction based on EST data.
Show »
Length:806
Mass (Da):93,294
Checksum:i4EA09B8F2B830917
GO
Isoform 3 (identifier: A3KGV1-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-41: MSASSSGGSP...PCGAPSVTVT → MKDRSSTPPL...LPKPSAASSQ

Note: No experimental confirmation available.
Show »
Length:825
Mass (Da):95,428
Checksum:i2C9CA2383DE85B81
GO
Isoform 4 (identifier: A3KGV1-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-41: MSASSSGGSP...PCGAPSVTVT → MKDRSSTPPL...LPKPSAASSQ
     281-281: E → EK

Note: No experimental confirmation available.
Show »
Length:826
Mass (Da):95,556
Checksum:i9C8D6973FDB5E2C9
GO
Isoform 5 (identifier: A3KGV1-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-41: MSASSSGGSP...PCGAPSVTVT → MKDRSSTPPL...LPKPSAASSQ
     638-657: IEHQGDKLEMAREKHQASQK → VRDWQKGSHELARAGARLPR
     658-830: Missing.

Note: No experimental confirmation available.
Show »
Length:652
Mass (Da):75,410
Checksum:i9545123A1A29B617
GO
Isoform 6 (identifier: A3KGV1-6) [UniParc]FASTAAdd to basket
Also known as: ODF2/2

The sequence of this isoform differs from the canonical sequence as follows:
     65-83: Missing.
     638-657: IEHQGDKLEMAREKHQASQK → VRDWQKGSHELARAGARLPR
     658-830: Missing.

Show »
Length:638
Mass (Da):73,389
Checksum:i23DC7851F0626B2E
GO
Isoform 7 (identifier: A3KGV1-7) [UniParc]FASTAAdd to basket
Also known as: ODF2/1

The sequence of this isoform differs from the canonical sequence as follows:
     1-47: Missing.
     638-657: IEHQGDKLEMAREKHQASQK → VRDWQKGSHELARAGARLPR
     658-830: Missing.

Show »
Length:610
Mass (Da):70,765
Checksum:i6ED88AB7A191A70B
GO

Computationally mapped potential isoform sequencesi

There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A3KGW0A3KGW0_MOUSE
Outer dense fiber protein 2
Odf2
701Annotation score:
A3KGV9A3KGV9_MOUSE
Outer dense fiber protein 2
Odf2
657Annotation score:
V9GXZ0V9GXZ0_MOUSE
Outer dense fiber protein 2
Odf2
680Annotation score:
F6Y325F6Y325_MOUSE
Outer dense fiber protein 2
Odf2
271Annotation score:
A3KGV3A3KGV3_MOUSE
Outer dense fiber protein 2
Odf2
138Annotation score:
A3KGV2A3KGV2_MOUSE
Outer dense fiber protein 2
Odf2
69Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti349L → W in AAH57001 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0276711 – 47Missing in isoform 7. 1 PublicationAdd BLAST47
Alternative sequenceiVSP_0276721 – 41MSASS…SVTVT → MKDRSSTPPLHVHVDENTPV HVHIKKLPKPSAASSQ in isoform 2, isoform 3, isoform 4 and isoform 5. 2 PublicationsAdd BLAST41
Alternative sequenceiVSP_02767365 – 83Missing in isoform 2 and isoform 6. 1 PublicationAdd BLAST19
Alternative sequenceiVSP_027674281E → EK in isoform 4. 1 Publication1
Alternative sequenceiVSP_027675638 – 657IEHQG…QASQK → VRDWQKGSHELARAGARLPR in isoform 5, isoform 6 and isoform 7. 2 PublicationsAdd BLAST20
Alternative sequenceiVSP_027676658 – 830Missing in isoform 5, isoform 6 and isoform 7. 2 PublicationsAdd BLAST173

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF000968 mRNA Translation: AAB65157.1
AF034105 mRNA Translation: AAB87525.1
AK143726 mRNA Translation: BAE25517.1
AK168415 mRNA Translation: BAE40330.1
AL928926 Genomic DNA No translation available.
BC057001 mRNA Translation: AAH57001.1
CCDSiCCDS15861.1 [A3KGV1-6]
CCDS50555.1 [A3KGV1-4]
CCDS50556.1 [A3KGV1-3]
CCDS50557.1 [A3KGV1-5]
PIRiT02298
T09400
RefSeqiNP_001106684.1, NM_001113213.1 [A3KGV1-3]
NP_001106685.1, NM_001113214.1 [A3KGV1-5]
NP_001171130.1, NM_001177659.1 [A3KGV1-4]
NP_001171132.1, NM_001177661.1
NP_038643.1, NM_013615.3 [A3KGV1-6]
XP_017171778.1, XM_017316289.1 [A3KGV1-1]
XP_017171779.1, XM_017316290.1 [A3KGV1-4]
XP_017171780.1, XM_017316291.1 [A3KGV1-4]
XP_017171781.1, XM_017316292.1 [A3KGV1-4]
XP_017171782.1, XM_017316293.1 [A3KGV1-3]
XP_017171793.1, XM_017316304.1 [A3KGV1-2]
XP_017171794.1, XM_017316305.1
UniGeneiMm.330116

Genome annotation databases

EnsembliENSMUST00000028128; ENSMUSP00000028128; ENSMUSG00000026790 [A3KGV1-6]
ENSMUST00000046571; ENSMUSP00000049272; ENSMUSG00000026790 [A3KGV1-3]
ENSMUST00000113755; ENSMUSP00000109384; ENSMUSG00000026790 [A3KGV1-5]
ENSMUST00000113756; ENSMUSP00000109385; ENSMUSG00000026790 [A3KGV1-3]
ENSMUST00000113757; ENSMUSP00000109386; ENSMUSG00000026790 [A3KGV1-2]
ENSMUST00000113759; ENSMUSP00000109388; ENSMUSG00000026790 [A3KGV1-4]
ENSMUST00000113763; ENSMUSP00000109392; ENSMUSG00000026790 [A3KGV1-6]
ENSMUST00000113764; ENSMUSP00000109393; ENSMUSG00000026790 [A3KGV1-6]
ENSMUST00000113765; ENSMUSP00000109394; ENSMUSG00000026790 [A3KGV1-1]
GeneIDi18286
KEGGimmu:18286
UCSCiuc008jaj.2 mouse [A3KGV1-5]
uc008jak.2 mouse [A3KGV1-3]
uc008jam.2 mouse [A3KGV1-6]
uc033hmh.1 mouse [A3KGV1-4]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF000968 mRNA Translation: AAB65157.1
AF034105 mRNA Translation: AAB87525.1
AK143726 mRNA Translation: BAE25517.1
AK168415 mRNA Translation: BAE40330.1
AL928926 Genomic DNA No translation available.
BC057001 mRNA Translation: AAH57001.1
CCDSiCCDS15861.1 [A3KGV1-6]
CCDS50555.1 [A3KGV1-4]
CCDS50556.1 [A3KGV1-3]
CCDS50557.1 [A3KGV1-5]
PIRiT02298
T09400
RefSeqiNP_001106684.1, NM_001113213.1 [A3KGV1-3]
NP_001106685.1, NM_001113214.1 [A3KGV1-5]
NP_001171130.1, NM_001177659.1 [A3KGV1-4]
NP_001171132.1, NM_001177661.1
NP_038643.1, NM_013615.3 [A3KGV1-6]
XP_017171778.1, XM_017316289.1 [A3KGV1-1]
XP_017171779.1, XM_017316290.1 [A3KGV1-4]
XP_017171780.1, XM_017316291.1 [A3KGV1-4]
XP_017171781.1, XM_017316292.1 [A3KGV1-4]
XP_017171782.1, XM_017316293.1 [A3KGV1-3]
XP_017171793.1, XM_017316304.1 [A3KGV1-2]
XP_017171794.1, XM_017316305.1
UniGeneiMm.330116

3D structure databases

ProteinModelPortaliA3KGV1
SMRiA3KGV1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201898, 3 interactors
IntActiA3KGV1, 3 interactors
STRINGi10090.ENSMUSP00000109388

PTM databases

iPTMnetiA3KGV1
PhosphoSitePlusiA3KGV1

2D gel databases

REPRODUCTION-2DPAGEiA3KGV1
IPI00130958

Proteomic databases

MaxQBiA3KGV1
PaxDbiA3KGV1
PeptideAtlasiA3KGV1
PRIDEiA3KGV1

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028128; ENSMUSP00000028128; ENSMUSG00000026790 [A3KGV1-6]
ENSMUST00000046571; ENSMUSP00000049272; ENSMUSG00000026790 [A3KGV1-3]
ENSMUST00000113755; ENSMUSP00000109384; ENSMUSG00000026790 [A3KGV1-5]
ENSMUST00000113756; ENSMUSP00000109385; ENSMUSG00000026790 [A3KGV1-3]
ENSMUST00000113757; ENSMUSP00000109386; ENSMUSG00000026790 [A3KGV1-2]
ENSMUST00000113759; ENSMUSP00000109388; ENSMUSG00000026790 [A3KGV1-4]
ENSMUST00000113763; ENSMUSP00000109392; ENSMUSG00000026790 [A3KGV1-6]
ENSMUST00000113764; ENSMUSP00000109393; ENSMUSG00000026790 [A3KGV1-6]
ENSMUST00000113765; ENSMUSP00000109394; ENSMUSG00000026790 [A3KGV1-1]
GeneIDi18286
KEGGimmu:18286
UCSCiuc008jaj.2 mouse [A3KGV1-5]
uc008jak.2 mouse [A3KGV1-3]
uc008jam.2 mouse [A3KGV1-6]
uc033hmh.1 mouse [A3KGV1-4]

Organism-specific databases

CTDi4957
MGIiMGI:1098824 Odf2

Phylogenomic databases

eggNOGiENOG410IE8V Eukaryota
ENOG4111XWH LUCA
GeneTreeiENSGT00530000063497
HOVERGENiHBG052808
InParanoidiA3KGV1
KOiK16479
OMAiNQAHIEV
OrthoDBiEOG091G13KV
PhylomeDBiA3KGV1
TreeFamiTF328605

Enzyme and pathway databases

ReactomeiR-MMU-2565942 Regulation of PLK1 Activity at G2/M Transition
R-MMU-380259 Loss of Nlp from mitotic centrosomes
R-MMU-380270 Recruitment of mitotic centrosome proteins and complexes
R-MMU-380320 Recruitment of NuMA to mitotic centrosomes
R-MMU-5620912 Anchoring of the basal body to the plasma membrane
R-MMU-8854518 AURKA Activation by TPX2

Miscellaneous databases

PROiPR:A3KGV1
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026790 Expressed in 292 organ(s), highest expression level in testis
CleanExiMM_ODF2
ExpressionAtlasiA3KGV1 baseline and differential
GenevisibleiA3KGV1 MM

Family and domain databases

InterProiView protein in InterPro
IPR026099 Odf2-rel
PANTHERiPTHR23162 PTHR23162, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiODFP2_MOUSE
AccessioniPrimary (citable) accession number: A3KGV1
Secondary accession number(s): A3KGV5
, A3KGV6, O35135, O35496, Q3TH68, Q3UP80, Q6PGI6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: April 3, 2007
Last modified: November 7, 2018
This is version 92 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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