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Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2

Gene

Plcb2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. This protein may be involved in the transduction of bitter taste stimuli (By similarity).By similarity

Miscellaneous

The receptor-mediated activation of PLC-beta-2 is most effectively mediated by one G-protein alpha subunit, alpha-16.By similarity

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei327PROSITE-ProRule annotation1
Metal bindingi328CalciumBy similarity1
Metal bindingi357CalciumBy similarity1
Metal bindingi359CalciumBy similarity1
Active sitei374PROSITE-ProRule annotation1
Metal bindingi408CalciumBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Transducer
Biological processLipid degradation, Lipid metabolism
LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-112043 PLC beta mediated events
R-MMU-1855204 Synthesis of IP3 and IP4 in the cytosol
R-MMU-399997 Acetylcholine regulates insulin secretion
R-MMU-4086398 Ca2+ pathway
R-MMU-416476 G alpha (q) signalling events
R-MMU-418217 G beta:gamma signalling through PLC beta
R-MMU-434316 Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion
R-MMU-500657 Presynaptic function of Kainate receptors

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 (EC:3.1.4.11)
Alternative name(s):
Phosphoinositide phospholipase C-beta-2
Phospholipase C-beta-2
Short name:
PLC-beta-2
Gene namesi
Name:Plcb2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:107465 Plcb2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002956831 – 11811-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2Add BLAST1181

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei950PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiA3KGF7
MaxQBiA3KGF7
PaxDbiA3KGF7
PeptideAtlasiA3KGF7
PRIDEiA3KGF7

PTM databases

iPTMnetiA3KGF7
PhosphoSitePlusiA3KGF7

Expressioni

Gene expression databases

BgeeiENSMUSG00000040061 Expressed in 125 organ(s), highest expression level in bone marrow
CleanExiMM_PLCB2
ExpressionAtlasiA3KGF7 baseline and differential
GenevisibleiA3KGF7 MM

Interactioni

Subunit structurei

Interacts with RAC1 (By similarity). Forms a complex composed of at least WDR26, a G-beta:gamma unit, and PLCB2 (By similarity).By similarity

Protein-protein interaction databases

CORUMiA3KGF7
IntActiA3KGF7, 6 interactors
STRINGi10090.ENSMUSP00000099583

Structurei

3D structure databases

ProteinModelPortaliA3KGF7
SMRiA3KGF7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini312 – 463PI-PLC X-boxPROSITE-ProRule annotationAdd BLAST152
Domaini547 – 663PI-PLC Y-boxPROSITE-ProRule annotationAdd BLAST117
Domaini670 – 768C2PROSITE-ProRule annotationAdd BLAST99

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili893 – 940Sequence analysisAdd BLAST48
Coiled coili974 – 1026Sequence analysisAdd BLAST53
Coiled coili1075 – 1141Sequence analysisAdd BLAST67

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi492 – 546Glu-richAdd BLAST55

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0169 Eukaryota
ENOG410XPSW LUCA
GeneTreeiENSGT00760000118936
HOGENOMiHOG000232046
HOVERGENiHBG053609
InParanoidiA3KGF7
KOiK05858
OMAiDEIFTSH
OrthoDBiEOG091G00XL
PhylomeDBiA3KGF7
TreeFamiTF313216

Family and domain databases

CDDicd13361 PH_PLC_beta, 1 hit
Gene3Di2.60.40.150, 1 hit
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR011992 EF-hand-dom_pair
IPR001192 PI-PLC_fam
IPR016280 PLC-beta
IPR028403 PLC-beta2
IPR014815 PLC-beta_C
IPR037862 PLC-beta_PH
IPR017946 PLC-like_Pdiesterase_TIM-brl
IPR015359 PLC_EF-hand-like
IPR000909 PLipase_C_PInositol-sp_X_dom
IPR001711 PLipase_C_Pinositol-sp_Y
PANTHERiPTHR10336 PTHR10336, 1 hit
PTHR10336:SF10 PTHR10336:SF10, 1 hit
PfamiView protein in Pfam
PF00168 C2, 1 hit
PF09279 EF-hand_like, 1 hit
PF00388 PI-PLC-X, 1 hit
PF00387 PI-PLC-Y, 1 hit
PF08703 PLC-beta_C, 1 hit
PIRSFiPIRSF000956 PLC-beta, 1 hit
PRINTSiPR00390 PHPHLIPASEC
SMARTiView protein in SMART
SM00239 C2, 1 hit
SM00148 PLCXc, 1 hit
SM00149 PLCYc, 1 hit
SUPFAMiSSF47473 SSF47473, 1 hit
SSF51695 SSF51695, 1 hit
PROSITEiView protein in PROSITE
PS50004 C2, 1 hit
PS50007 PIPLC_X_DOMAIN, 1 hit
PS50008 PIPLC_Y_DOMAIN, 1 hit

Sequences (4+)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 4 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: A3KGF7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSLLNPVLLP PNVKAYLSQG ERFIKWDDET SIASPVILRV DPKGYYLYWT
60 70 80 90 100
YQNQEMEFLD VTSIRDTRFG KFAKIPKSQK LREVFNMDFP DNHFLLKTLT
110 120 130 140 150
VVSGPDMVDL TFYNFVSYKE NVGKDWAEDV LALAKHPMTV NAPRSTFLDK
160 170 180 190 200
ILVKLKMQLN PEGKIPVKNF FQMFPADRKR VEAALGACHL AKGKNDAINP
210 220 230 240 250
EDFPESVYKS FLMSLCPRPE IDEIFTSYHS KAKPYMTKEH LTKFINQKQR
260 270 280 290 300
DPRLNSLLFP PARPEQVQVL IDKYEPSGIN VQRGQLSPEG MVWFLCGPEN
310 320 330 340 350
SVLAHDTLLI HQDMTQPLNH YFINSSHNTY LTAGQFSGLS SAEMYRQVLL
360 370 380 390 400
SGCRCVELDC WKGKPPDEEP IITHGFTMTT DILFKEAIEA IAESAFKTSP
410 420 430 440 450
YPVILSFENH VDSPRQQAKM AEYCRSMFGE TLLTDPLENF PLKPGIPLPS
460 470 480 490 500
PEDLRGKILI KNKKNQFSGP ASPSKKPGGV AEGSLPSSVP VEEDTGWTAE
510 520 530 540 550
DRTEVEEEEV VEEEEEEESG NLDEEEIKKM QSDEGTAGLE VTAYEEMSSL
560 570 580 590 600
VNYIQPTKFI SFEFSAQKNR SYVVSSFTEL KAYELLSKAS MQFVDYNKRQ
610 620 630 640 650
MSRVYPKGTR MDSSNYMPQM FWNAGCQMVA LNFQTMDLPM QQNMALFEFN
660 670 680 690 700
GQSGYLLKHE FMRRLDKQFN PFSVDRIDVV VATTLSITII SGQFLSERSV
710 720 730 740 750
RTYVEVELFG LPGDPKRRYR TKLSPTANSI NPVWKEEPFI FEKILMPELA
760 770 780 790 800
SLRIAVMEEG SKFLGHRIIP INALHSGYHH LCLRSESNMA LTMPALFVFL
810 820 830 840 850
EMKDYIPDTW ADLTVALANP IKYFNAQDKK SVKLKGVTGS LPEKLFSGTP
860 870 880 890 900
VASQSNGAPV SAGNGSTAPG TKATGEEATK EVTEPQTASL EELRELKGVV
910 920 930 940 950
KLQRRHEKEL RELERRGARR WEELLQRGAA QLAELQTQAA GCKLRPGKGS
960 970 980 990 1000
RKKRTLPCEE TVVAPSEPHD RADPRVQELK DRLEQELQQQ GEEQYRSVLK
1010 1020 1030 1040 1050
RKEQHVTEQI AKMMELAREK QAAELKTFKE TSETDTKEMK KKLEAKRLER
1060 1070 1080 1090 1100
IQAMTKVTTD KVAQERLKRE INNSHIQEVV QAVKQMTETL ERHQEKLEER
1110 1120 1130 1140 1150
QTACLEQIQA MEKQFQEKAL AEYEAKMKGL EAEVKESVRA YFKDCFPTEA
1160 1170 1180
EDKPERSCEA SEESCPQEPL VSKADTQESR L
Length:1,181
Mass (Da):134,546
Last modified:April 3, 2007 - v1
Checksum:i8E23BD29391ABE4E
GO
Isoform 2 (identifier: A3KGF7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     55-77: Missing.
     689-702: IISGQFLSERSVRT → ARPRADKWLGGLGS
     703-1181: Missing.

Show »
Length:679
Mass (Da):77,235
Checksum:i671A64A042AE7024
GO
Isoform 3 (identifier: A3KGF7-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     689-702: IISGQFLSERSVRT → ARPRADKWLGGLGS
     703-1181: Missing.

Show »
Length:702
Mass (Da):79,946
Checksum:iB07FB842B2C8CC73
GO
Isoform 4 (identifier: A3KGF7-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-55: Missing.
     637-688: DLPMQQNMAL...VVVATTLSIT → GKNTSKTREP...DPYSSLDKEL
     689-1181: Missing.

Show »
Length:633
Mass (Da):71,354
Checksum:i30D14CB6B1901634
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9PYI3E9PYI3_MOUSE
1-phosphatidylinositol 4,5-bisphosp...
Plcb2
1,158Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0269811 – 55Missing in isoform 4. 1 PublicationAdd BLAST55
Alternative sequenceiVSP_02698255 – 77Missing in isoform 2. 1 PublicationAdd BLAST23
Alternative sequenceiVSP_026983637 – 688DLPMQ…TLSIT → GKNTSKTREPSPGPLTTSPE PSPGPCIPRCPPSEPSSGPC LSDPYSSLDKEL in isoform 4. 1 PublicationAdd BLAST52
Alternative sequenceiVSP_026984689 – 1181Missing in isoform 4. 1 PublicationAdd BLAST493
Alternative sequenceiVSP_026985689 – 702IISGQ…RSVRT → ARPRADKWLGGLGS in isoform 2 and isoform 3. 1 PublicationAdd BLAST14
Alternative sequenceiVSP_026986703 – 1181Missing in isoform 2 and isoform 3. 1 PublicationAdd BLAST479

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY902324 Genomic DNA Translation: AAX90609.1
AK045469 mRNA Translation: BAC32384.1
AK169442 mRNA Translation: BAE41180.1
AL772255 Genomic DNA Translation: CAM45993.1
AL772255 Genomic DNA Translation: CAM45994.1
AL772255 Genomic DNA Translation: CAM45995.1
CCDSiCCDS16582.1 [A3KGF7-1]
RefSeqiNP_001277719.1, NM_001290790.1
NP_808236.2, NM_177568.2 [A3KGF7-1]
UniGeneiMm.215156

Genome annotation databases

EnsembliENSMUST00000102524; ENSMUSP00000099583; ENSMUSG00000040061 [A3KGF7-1]
GeneIDi18796
KEGGimmu:18796
UCSCiuc008lsi.1 mouse [A3KGF7-1]
uc008lsk.1 mouse [A3KGF7-2]
uc008lsl.1 mouse [A3KGF7-4]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY902324 Genomic DNA Translation: AAX90609.1
AK045469 mRNA Translation: BAC32384.1
AK169442 mRNA Translation: BAE41180.1
AL772255 Genomic DNA Translation: CAM45993.1
AL772255 Genomic DNA Translation: CAM45994.1
AL772255 Genomic DNA Translation: CAM45995.1
CCDSiCCDS16582.1 [A3KGF7-1]
RefSeqiNP_001277719.1, NM_001290790.1
NP_808236.2, NM_177568.2 [A3KGF7-1]
UniGeneiMm.215156

3D structure databases

ProteinModelPortaliA3KGF7
SMRiA3KGF7
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

CORUMiA3KGF7
IntActiA3KGF7, 6 interactors
STRINGi10090.ENSMUSP00000099583

PTM databases

iPTMnetiA3KGF7
PhosphoSitePlusiA3KGF7

Proteomic databases

EPDiA3KGF7
MaxQBiA3KGF7
PaxDbiA3KGF7
PeptideAtlasiA3KGF7
PRIDEiA3KGF7

Protocols and materials databases

DNASUi18796
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000102524; ENSMUSP00000099583; ENSMUSG00000040061 [A3KGF7-1]
GeneIDi18796
KEGGimmu:18796
UCSCiuc008lsi.1 mouse [A3KGF7-1]
uc008lsk.1 mouse [A3KGF7-2]
uc008lsl.1 mouse [A3KGF7-4]

Organism-specific databases

CTDi5330
MGIiMGI:107465 Plcb2

Phylogenomic databases

eggNOGiKOG0169 Eukaryota
ENOG410XPSW LUCA
GeneTreeiENSGT00760000118936
HOGENOMiHOG000232046
HOVERGENiHBG053609
InParanoidiA3KGF7
KOiK05858
OMAiDEIFTSH
OrthoDBiEOG091G00XL
PhylomeDBiA3KGF7
TreeFamiTF313216

Enzyme and pathway databases

ReactomeiR-MMU-112043 PLC beta mediated events
R-MMU-1855204 Synthesis of IP3 and IP4 in the cytosol
R-MMU-399997 Acetylcholine regulates insulin secretion
R-MMU-4086398 Ca2+ pathway
R-MMU-416476 G alpha (q) signalling events
R-MMU-418217 G beta:gamma signalling through PLC beta
R-MMU-434316 Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion
R-MMU-500657 Presynaptic function of Kainate receptors

Miscellaneous databases

ChiTaRSiPlcb2 mouse
PROiPR:A3KGF7
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000040061 Expressed in 125 organ(s), highest expression level in bone marrow
CleanExiMM_PLCB2
ExpressionAtlasiA3KGF7 baseline and differential
GenevisibleiA3KGF7 MM

Family and domain databases

CDDicd13361 PH_PLC_beta, 1 hit
Gene3Di2.60.40.150, 1 hit
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR011992 EF-hand-dom_pair
IPR001192 PI-PLC_fam
IPR016280 PLC-beta
IPR028403 PLC-beta2
IPR014815 PLC-beta_C
IPR037862 PLC-beta_PH
IPR017946 PLC-like_Pdiesterase_TIM-brl
IPR015359 PLC_EF-hand-like
IPR000909 PLipase_C_PInositol-sp_X_dom
IPR001711 PLipase_C_Pinositol-sp_Y
PANTHERiPTHR10336 PTHR10336, 1 hit
PTHR10336:SF10 PTHR10336:SF10, 1 hit
PfamiView protein in Pfam
PF00168 C2, 1 hit
PF09279 EF-hand_like, 1 hit
PF00388 PI-PLC-X, 1 hit
PF00387 PI-PLC-Y, 1 hit
PF08703 PLC-beta_C, 1 hit
PIRSFiPIRSF000956 PLC-beta, 1 hit
PRINTSiPR00390 PHPHLIPASEC
SMARTiView protein in SMART
SM00239 C2, 1 hit
SM00148 PLCXc, 1 hit
SM00149 PLCYc, 1 hit
SUPFAMiSSF47473 SSF47473, 1 hit
SSF51695 SSF51695, 1 hit
PROSITEiView protein in PROSITE
PS50004 C2, 1 hit
PS50007 PIPLC_X_DOMAIN, 1 hit
PS50008 PIPLC_Y_DOMAIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPLCB2_MOUSE
AccessioniPrimary (citable) accession number: A3KGF7
Secondary accession number(s): Q2M4J1, Q3TER8, Q8BI81
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: April 3, 2007
Last modified: November 7, 2018
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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