UniProtKB - A3EXD7 (NCAP_BCHK5)
Protein
Nucleoprotein
Gene
N
Organism
Bat coronavirus HKU5 (BtCoV) (BtCoV/HKU5/2004)
Status
Functioni
Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication.UniRule annotation
GO - Molecular functioni
- RNA binding Source: UniProtKB-UniRule
Keywordsi
Molecular function | Ribonucleoprotein, RNA-binding, Viral nucleoprotein |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: NucleoproteinUniRule annotationAlternative name(s): Nucleocapsid proteinUniRule annotation Short name: NCUniRule annotation Short name: Protein NUniRule annotation |
Gene namesi | Name:NUniRule annotation ORF Names:6 |
Organismi | Bat coronavirus HKU5 (BtCoV) (BtCoV/HKU5/2004) |
Taxonomic identifieri | 694008 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Orthornavirae › Pisuviricota › Pisoniviricetes › Nidovirales › Cornidovirineae › Coronaviridae › Orthocoronavirinae › Betacoronavirus › Merbecovirus |
Virus hosti | Pipistrellus abramus (Japanese pipistrelle) (Pipistrellus javanicus abramus) [TaxID: 105295] |
Proteomesi |
|
Subcellular locationi
- Virion UniRule annotation
- Host endoplasmic reticulum-Golgi intermediate compartment UniRule annotation
- Host Golgi apparatus UniRule annotation Note: Located inside the virion, complexed with the viral RNA. Probably associates with ER-derived membranes where it participates in viral RNA synthesis and virus budding.UniRule annotation
GO - Cellular componenti
- host cell endoplasmic reticulum-Golgi intermediate compartment Source: UniProtKB-SubCell
- host cell Golgi apparatus Source: UniProtKB-SubCell
- viral nucleocapsid Source: UniProtKB-UniRule
Keywords - Cellular componenti
Host Golgi apparatus, VirionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000290260 | 1 – 427 | NucleoproteinAdd BLAST | 427 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 132 | Phosphoserine; by hostUniRule annotation | 1 | |
Modified residuei | 147 | Phosphothreonine; by hostUniRule annotation | 1 |
Post-translational modificationi
ADP-ribosylated. The ADP-ribosylation is retained in the virion during infection.UniRule annotation
Phosphorylated on serine and threonine residues.UniRule annotation
Keywords - PTMi
ADP-ribosylation, PhosphoproteinInteractioni
Subunit structurei
Homooligomer. Both monomeric and oligomeric forms interact with RNA.
Interacts with protein M.
Interacts with NSP3; this interaction serves to tether the genome to the newly translated replicase-transcriptase complex at a very early stage of infection.
UniRule annotationFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 38 – 163 | CoV N NTDPROSITE-ProRule annotationAdd BLAST | 126 | |
Domaini | 238 – 361 | CoV N CTDPROSITE-ProRule annotationAdd BLAST | 124 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 34 – 172 | RNA-bindingUniRule annotationAdd BLAST | 139 | |
Regioni | 249 – 359 | DimerizationUniRule annotationAdd BLAST | 111 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 168 – 197 | Ser-richAdd BLAST | 30 |
Sequence similaritiesi
Belongs to the betacoronavirus nucleocapsid protein family.UniRule annotation
Family and domain databases
HAMAPi | MF_04096, BETA_CORONA_NCAP, 1 hit |
InterProi | View protein in InterPro IPR043505, NCAP_bCoV IPR001218, Nucleocap_CoV IPR037179, Nucleocapsid_C IPR037195, Nucleocapsid_N |
Pfami | View protein in Pfam PF00937, CoV_nucleocap, 1 hit |
PIRSFi | PIRSF003888, Corona_nucleocap, 1 hit |
SUPFAMi | SSF103068, SSF103068, 1 hit SSF110304, SSF110304, 1 hit |
PROSITEi | View protein in PROSITE PS51929, COV_N_CTD, 1 hit PS51928, COV_N_NTD, 1 hit |
i Sequence
Sequence statusi: Complete.
A3EXD7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MATPAPPRAV VFANDNETPT NSQRSGRPRT KPRPAPNTTV SWFTGLTQHG
60 70 80 90 100
KQPLAFPPGQ GVPLNANSTP AQNAGYWRRQ DRKINTGNGT KPLAPRWYFY
110 120 130 140 150
YTGTGPEANL PFRSVKDGII WVHENGATDA PSVFGTRNPA NDPAIVTQFA
160 170 180 190 200
PGTTLPKNFH IEGTGGNSQS SSRASSRSSS RSSSRNGRSN NSSRNASPAP
210 220 230 240 250
HGVGDVVGAG TLSVLLDLQK RLADLEAGKG NKQPKVITKK DAQAAKQKMR
260 270 280 290 300
HKRVATKGYN VVQAFGMRGP GPLQSNFGDM QYNKLGTEDP RWPQIAELAP
310 320 330 340 350
SASAFMSTSQ FKVTHQSNDE NGEPVYFLSY SGAIKLDPKN PNYNKWMEIL
360 370 380 390 400
DANIDAYKSF PKKERKQKPS GDDAATAPAT SQMEDVPELP PKQQRKKRVV
410 420
QGSIPQRSAG VPSFEDVVDA IFPDSEA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | EF065509 Genomic RNA Translation: ABN10882.1 |
RefSeqi | YP_001039969.1, NC_009020.1 |
Genome annotation databases
GeneIDi | 4836000 |
KEGGi | vg:4836000 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | EF065509 Genomic RNA Translation: ABN10882.1 |
RefSeqi | YP_001039969.1, NC_009020.1 |
3D structure databases
SMRi | A3EXD7 |
ModBasei | Search... |
Genome annotation databases
GeneIDi | 4836000 |
KEGGi | vg:4836000 |
Family and domain databases
HAMAPi | MF_04096, BETA_CORONA_NCAP, 1 hit |
InterProi | View protein in InterPro IPR043505, NCAP_bCoV IPR001218, Nucleocap_CoV IPR037179, Nucleocapsid_C IPR037195, Nucleocapsid_N |
Pfami | View protein in Pfam PF00937, CoV_nucleocap, 1 hit |
PIRSFi | PIRSF003888, Corona_nucleocap, 1 hit |
SUPFAMi | SSF103068, SSF103068, 1 hit SSF110304, SSF110304, 1 hit |
PROSITEi | View protein in PROSITE PS51929, COV_N_CTD, 1 hit PS51928, COV_N_NTD, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NCAP_BCHK5 | |
Accessioni | A3EXD7Primary (citable) accession number: A3EXD7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 12, 2007 |
Last sequence update: | March 20, 2007 | |
Last modified: | December 2, 2020 | |
This is version 74 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families