Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Pyridoxal 5'-phosphate synthase subunit PdxT

Gene

pdxT

Organism
Methanoculleus marisnigri (strain ATCC 35101 / DSM 1498 / JR1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the biosynthesis of pyridoxal 5'-phosphate. The resulting ammonia molecule is channeled to the active site of PdxS.UniRule annotation

Catalytic activityi

D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = pyridoxal 5'-phosphate + L-glutamate + 3 H2O + phosphate.UniRule annotation
L-glutamine + H2O = L-glutamate + NH3.UniRule annotation

Pathwayi: pyridoxal 5'-phosphate biosynthesis

This protein is involved in the pathway pyridoxal 5'-phosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway pyridoxal 5'-phosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei82NucleophileUniRule annotation1
Binding sitei109L-glutamineUniRule annotation1
Active sitei173Charge relay systemUniRule annotation1
Active sitei175Charge relay systemUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Lyase
LigandPyridoxal phosphate

Enzyme and pathway databases

BioCyciMMAR368407:G1G85-9-MONOMER
UniPathwayiUPA00245

Names & Taxonomyi

Protein namesi
Recommended name:
Pyridoxal 5'-phosphate synthase subunit PdxTUniRule annotation (EC:4.3.3.6UniRule annotation)
Alternative name(s):
Pdx2UniRule annotation
Pyridoxal 5'-phosphate synthase glutaminase subunitUniRule annotation (EC:3.5.1.2UniRule annotation)
Gene namesi
Name:pdxTUniRule annotation
Ordered Locus Names:Memar_0010
OrganismiMethanoculleus marisnigri (strain ATCC 35101 / DSM 1498 / JR1)
Taxonomic identifieri368407 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanomicrobiaceaeMethanoculleus
Proteomesi
  • UP000002146 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002930191 – 192Pyridoxal 5'-phosphate synthase subunit PdxTAdd BLAST192

Interactioni

Subunit structurei

In the presence of PdxS, forms a dodecamer of heterodimers. Only shows activity in the heterodimer.UniRule annotation

Protein-protein interaction databases

STRINGi368407.Memar_0010

Structurei

3D structure databases

ProteinModelPortaliA3CRE5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni53 – 55L-glutamine bindingUniRule annotation3
Regioni137 – 138L-glutamine bindingUniRule annotation2

Sequence similaritiesi

Belongs to the glutaminase PdxT/SNO family.UniRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiarCOG00034 Archaea
COG0311 LUCA
HOGENOMiHOG000039949
KOiK08681
OMAiVFIRAPI
OrthoDBiPOG093Z0AY8

Family and domain databases

CDDicd01749 GATase1_PB, 1 hit
Gene3Di3.40.50.880, 1 hit
HAMAPiMF_01615 PdxT, 1 hit
InterProiView protein in InterPro
IPR029062 Class_I_gatase-like
IPR002161 PdxT/SNO
IPR021196 PdxT/SNO_CS
PANTHERiPTHR31559 PTHR31559, 1 hit
PfamiView protein in Pfam
PF01174 SNO, 1 hit
PIRSFiPIRSF005639 Glut_amidoT_SNO, 1 hit
SUPFAMiSSF52317 SSF52317, 1 hit
TIGRFAMsiTIGR03800 PLP_synth_Pdx2, 1 hit
PROSITEiView protein in PROSITE
PS01236 PDXT_SNO_1, 1 hit
PS51130 PDXT_SNO_2, 1 hit

Sequencei

Sequence statusi: Complete.

A3CRE5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGVKVGVLAL QGDVAEHIAA FREALAERPG SSVAPIRRAE DLADLDALAI
60 70 80 90 100
PGGESTTISR LMGKNGLYEP VAEFEGGVFA TCAGMVLSAD RVDDPRVRPL
110 120 130 140 150
SLIPMHVARN AFGRQVDSRE TMLEVAGLAE PFRAVFIRAP VATEAGDGVE
160 170 180 190
VLARIPEGIV AVRHGRHMAF AFHPELGGDL RLHRVFLEGL EV
Length:192
Mass (Da):20,498
Last modified:March 20, 2007 - v1
Checksum:iD46C8B7AA99708C0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000562 Genomic DNA Translation: ABN55945.1
RefSeqiWP_011842866.1, NC_009051.1

Genome annotation databases

EnsemblBacteriaiABN55945; ABN55945; Memar_0010
GeneIDi4847746
KEGGimem:Memar_0010

Similar proteinsi

Entry informationi

Entry nameiPDXT_METMJ
AccessioniPrimary (citable) accession number: A3CRE5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: March 20, 2007
Last modified: July 18, 2018
This is version 64 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health