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Protein

Diphthine synthase

Gene

dphB

Organism
Methanocorpusculum labreanum (strain ATCC 43576 / DSM 4855 / Z)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the trimethylation of the amino group of the modified target histidine residue in translation elongation factor 2 (EF-2), to form an intermediate called diphthine. The three successive methylation reactions represent the second step of diphthamide biosynthesis.UniRule annotation

Catalytic activityi

3 S-adenosyl-L-methionine + 2-((3S)-3-carboxy-3-aminopropyl)-L-histidine-[translation elongation factor 2] = 3 S-adenosyl-L-homocysteine + diphthine-[translation elongation factor 2].UniRule annotation

Pathwayi: peptidyl-diphthamide biosynthesis

This protein is involved in the pathway peptidyl-diphthamide biosynthesis, which is part of Protein modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway peptidyl-diphthamide biosynthesis and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei9S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei85S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei88S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei165S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei204S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei229S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMLAB410358:G1G83-406-MONOMER
UniPathwayi
UPA00559

Names & Taxonomyi

Protein namesi
Recommended name:
Diphthine synthaseUniRule annotation (EC:2.1.1.98UniRule annotation)
Alternative name(s):
Diphthamide biosynthesis methyltransferaseUniRule annotation
Gene namesi
Name:dphBUniRule annotation
Ordered Locus Names:Mlab_0386
OrganismiMethanocorpusculum labreanum (strain ATCC 43576 / DSM 4855 / Z)
Taxonomic identifieri410358 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaStenosarchaea groupMethanomicrobiaMethanomicrobialesMethanocorpusculaceaeMethanocorpusculum
Proteomesi
  • UP000000365 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000648201 – 252Diphthine synthaseAdd BLAST252

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi410358.Mlab_0386

Structurei

3D structure databases

ProteinModelPortaliA2SQF6
SMRiA2SQF6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni113 – 114S-adenosyl-L-methionine bindingUniRule annotation2

Sequence similaritiesi

Belongs to the diphthine synthase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04161 Archaea
COG1798 LUCA
HOGENOMiHOG000205302
KOiK20215
OMAiGLQNYRF
OrthoDBiPOG093Z0BZB

Family and domain databases

CDDicd11647 Diphthine_synthase, 1 hit
Gene3Di3.30.950.10, 1 hit
3.40.1010.10, 1 hit
HAMAPiMF_01084 Diphthine_synth, 1 hit
InterProiView protein in InterPro
IPR000878 4pyrrol_Mease
IPR035996 4pyrrol_Methylase_sf
IPR014777 4pyrrole_Mease_sub1
IPR014776 4pyrrole_Mease_sub2
IPR004551 Dphthn_synthase
PANTHERiPTHR10882:SF0 PTHR10882:SF0, 1 hit
PfamiView protein in Pfam
PF00590 TP_methylase, 1 hit
PIRSFiPIRSF036432 Diphthine_synth, 1 hit
SUPFAMiSSF53790 SSF53790, 1 hit
TIGRFAMsiTIGR00522 dph5, 1 hit

Sequencei

Sequence statusi: Complete.

A2SQF6-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLTFIGLGLF DEYDVSVRGL DAIKSADTVF LEVYTSVLMG APIERLEAFY
60 70 80 90 100
GKKITPLYRE DVEIHADKIL DAAEFGNAVF LTAGDSMVAT THSDLRIRAA
110 120 130 140 150
DRNIPTTIIH GASITTAVCG LSGLQNYRFG KSVSVPFPYG KWFPMTPIEV
160 170 180 190 200
ISANLKENLH TLVFLDIQKD KERYMKISEA VDLLEEQARR VDAGIPLYVG
210 220 230 240 250
IARAGSPEPT VHAGNAEEMK AFDFGSPLHI LIVPATLHEI EREYLERFAG

LC
Length:252
Mass (Da):27,852
Last modified:March 6, 2007 - v1
Checksum:i4BD7E5DB16CAF8E1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000559 Genomic DNA Translation: ABN06562.1
RefSeqiWP_011832763.1, NC_008942.1

Genome annotation databases

EnsemblBacteriaiABN06562; ABN06562; Mlab_0386
GeneIDi4794720
KEGGimla:Mlab_0386

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000559 Genomic DNA Translation: ABN06562.1
RefSeqiWP_011832763.1, NC_008942.1

3D structure databases

ProteinModelPortaliA2SQF6
SMRiA2SQF6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi410358.Mlab_0386

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABN06562; ABN06562; Mlab_0386
GeneIDi4794720
KEGGimla:Mlab_0386

Phylogenomic databases

eggNOGiarCOG04161 Archaea
COG1798 LUCA
HOGENOMiHOG000205302
KOiK20215
OMAiGLQNYRF
OrthoDBiPOG093Z0BZB

Enzyme and pathway databases

UniPathwayi
UPA00559

BioCyciMLAB410358:G1G83-406-MONOMER

Family and domain databases

CDDicd11647 Diphthine_synthase, 1 hit
Gene3Di3.30.950.10, 1 hit
3.40.1010.10, 1 hit
HAMAPiMF_01084 Diphthine_synth, 1 hit
InterProiView protein in InterPro
IPR000878 4pyrrol_Mease
IPR035996 4pyrrol_Methylase_sf
IPR014777 4pyrrole_Mease_sub1
IPR014776 4pyrrole_Mease_sub2
IPR004551 Dphthn_synthase
PANTHERiPTHR10882:SF0 PTHR10882:SF0, 1 hit
PfamiView protein in Pfam
PF00590 TP_methylase, 1 hit
PIRSFiPIRSF036432 Diphthine_synth, 1 hit
SUPFAMiSSF53790 SSF53790, 1 hit
TIGRFAMsiTIGR00522 dph5, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDPHB_METLZ
AccessioniPrimary (citable) accession number: A2SQF6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: March 6, 2007
Last modified: October 10, 2018
This is version 67 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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