Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

tRNA wybutosine-synthesizing protein 5

Gene

TYW5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

tRNA hydroxylase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Catalyzes the hydroxylation of 7-(a-amino-a-carboxypropyl)wyosine (yW-72) into undermodified hydroxywybutosine (OHyW*). OHyW* being further transformed into hydroxywybutosine (OHyW) by LCMT2/TYW4. OHyW is a derivative of wybutosine found in higher eukaryotes.1 Publication

Catalytic activityi

7-(3-amino-3-carboxypropyl)wyosine(37) in tRNA(Phe) + 2-oxoglutarate + O2 = 7-(2-hydroxy-3-amino-3-carboxypropyl)wyosine(37) in tRNA(Phe) + succinate + CO2.

Cofactori

Fe2+1 PublicationNote: Binds 1 Fe2+ ion per subunit.1 Publication

Pathwayi: wybutosine-tRNA(Phe) biosynthesis

This protein is involved in the pathway wybutosine-tRNA(Phe) biosynthesis, which is part of tRNA modification.2 Publications
View all proteins of this organism that are known to be involved in the pathway wybutosine-tRNA(Phe) biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei1062-oxoglutarate1 Publication1
Metal bindingi160Iron; catalytic1
Metal bindingi162Iron; catalytic1
Binding sitei1662-oxoglutarate1 Publication1
Binding sitei1752-oxoglutarate1 Publication1
Metal bindingi235Iron; catalytic1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDioxygenase, Oxidoreductase
Biological processtRNA processing
LigandIron, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:ENSG00000162971-MONOMER
BRENDAi1.14.11.42 2681
ReactomeiR-HSA-6782861 Synthesis of wybutosine at G37 of tRNA(Phe)
UniPathwayi
UPA00375

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA wybutosine-synthesizing protein 5 (EC:1.14.11.42)
Short name:
hTYW5
Alternative name(s):
tRNA(Phe) (7-(3-amino-3-carboxypropyl)wyosine(37)-C(2))-hydroxylase
Gene namesi
Name:TYW5
Synonyms:C2orf60
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000162971.10
HGNCiHGNC:26754 TYW5
neXtProtiNX_A2RUC4

Subcellular locationi

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi108R → A: Abolishes enzyme activity and ability to bind tRNA. 1 Publication1
Mutagenesisi149R → A: Abolishes enzyme activity and ability to bind tRNA. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000162971
PharmGKBiPA162379298

Polymorphism and mutation databases

BioMutaiTYW5

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003092741 – 315tRNA wybutosine-synthesizing protein 5Add BLAST315

Proteomic databases

EPDiA2RUC4
MaxQBiA2RUC4
PaxDbiA2RUC4
PRIDEiA2RUC4
ProteomicsDBi511
512 [A2RUC4-2]

PTM databases

iPTMnetiA2RUC4
PhosphoSitePlusiA2RUC4

Expressioni

Gene expression databases

BgeeiENSG00000162971 Expressed in 160 organ(s), highest expression level in tendon of biceps brachii
CleanExiHS_C2orf60
ExpressionAtlasiA2RUC4 baseline and differential
GenevisibleiA2RUC4 HS

Organism-specific databases

HPAiHPA028294
HPA045803

Interactioni

Subunit structurei

Homodimer.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi126193, 10 interactors
STRINGi9606.ENSP00000346627

Structurei

Secondary structure

1315
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliA2RUC4
SMRiA2RUC4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini102 – 267JmjCPROSITE-ProRule annotationAdd BLAST166

Sequence similaritiesi

Belongs to the TYW5 family.Curated

Phylogenomic databases

eggNOGiKOG2132 Eukaryota
ENOG410XQDR LUCA
GeneTreeiENSGT00530000062914
HOVERGENiHBG096249
InParanoidiA2RUC4
KOiK18066
OMAiPLYDDRP
OrthoDBiEOG091G0B98
PhylomeDBiA2RUC4
TreeFamiTF332364

Family and domain databases

InterProiView protein in InterPro
IPR003347 JmjC_dom
IPR029612 TYW5
PANTHERiPTHR12461:SF50 PTHR12461:SF50, 1 hit
SMARTiView protein in SMART
SM00558 JmjC, 1 hit
PROSITEiView protein in PROSITE
PS51184 JMJC, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: A2RUC4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAGQHLPVPR LEGVSREQFM QHLYPQRKPL VLEGIDLGPC TSKWTVDYLS
60 70 80 90 100
QVGGKKEVKI HVAAVAQMDF ISKNFVYRTL PFDQLVQRAA EEKHKEFFVS
110 120 130 140 150
EDEKYYLRSL GEDPRKDVAD IRKQFPLLKG DIKFPEFFKE EQFFSSVFRI
160 170 180 190 200
SSPGLQLWTH YDVMDNLLIQ VTGKKRVVLF SPRDAQYLYL KGTKSEVLNI
210 220 230 240 250
DNPDLAKYPL FSKARRYECS LEAGDVLFIP ALWFHNVISE EFGVGVNIFW
260 270 280 290 300
KHLPSECYDK TDTYGNKDPT AASRAAQILD RALKTLAELP EEYRDFYARR
310
MVLHIQDKAY SKNSE
Length:315
Mass (Da):36,548
Last modified:March 6, 2007 - v1
Checksum:iF291C48E8CA729FE
GO
Isoform 2 (identifier: A2RUC4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-163: Missing.

Note: No experimental confirmation available.
Show »
Length:152
Mass (Da):17,526
Checksum:i3FF012BC9E0CAED4
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A8KAJ9A8KAJ9_HUMAN
cDNA FLJ78342
TYW5
121Annotation score:

Sequence cautioni

The sequence AAH63502 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03692650S → G. Corresponds to variant dbSNP:rs10497844Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0291061 – 163Missing in isoform 2. 1 PublicationAdd BLAST163

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK095272 mRNA Translation: BAC04517.1
AK298977 mRNA Translation: BAG61071.1
AC097717 Genomic DNA Translation: AAY24162.1
CH471063 Genomic DNA Translation: EAW70191.1
BC063502 mRNA Translation: AAH63502.1 Different initiation.
BC132835 mRNA Translation: AAI32836.1
BC136837 mRNA Translation: AAI36838.1
CCDSiCCDS42795.1 [A2RUC4-1]
RefSeqiNP_001034782.1, NM_001039693.2 [A2RUC4-1]
UniGeneiHs.204619

Genome annotation databases

EnsembliENST00000354611; ENSP00000346627; ENSG00000162971 [A2RUC4-1]
GeneIDi129450
KEGGihsa:129450
UCSCiuc002uvi.5 human [A2RUC4-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK095272 mRNA Translation: BAC04517.1
AK298977 mRNA Translation: BAG61071.1
AC097717 Genomic DNA Translation: AAY24162.1
CH471063 Genomic DNA Translation: EAW70191.1
BC063502 mRNA Translation: AAH63502.1 Different initiation.
BC132835 mRNA Translation: AAI32836.1
BC136837 mRNA Translation: AAI36838.1
CCDSiCCDS42795.1 [A2RUC4-1]
RefSeqiNP_001034782.1, NM_001039693.2 [A2RUC4-1]
UniGeneiHs.204619

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3AL5X-ray2.50A/B/C/D1-315[»]
3AL6X-ray2.80A/B/C/D1-315[»]
ProteinModelPortaliA2RUC4
SMRiA2RUC4
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi126193, 10 interactors
STRINGi9606.ENSP00000346627

PTM databases

iPTMnetiA2RUC4
PhosphoSitePlusiA2RUC4

Polymorphism and mutation databases

BioMutaiTYW5

Proteomic databases

EPDiA2RUC4
MaxQBiA2RUC4
PaxDbiA2RUC4
PRIDEiA2RUC4
ProteomicsDBi511
512 [A2RUC4-2]

Protocols and materials databases

DNASUi129450
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000354611; ENSP00000346627; ENSG00000162971 [A2RUC4-1]
GeneIDi129450
KEGGihsa:129450
UCSCiuc002uvi.5 human [A2RUC4-1]

Organism-specific databases

CTDi129450
EuPathDBiHostDB:ENSG00000162971.10
GeneCardsiTYW5
H-InvDBiHIX0002719
HGNCiHGNC:26754 TYW5
HPAiHPA028294
HPA045803
neXtProtiNX_A2RUC4
OpenTargetsiENSG00000162971
PharmGKBiPA162379298
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2132 Eukaryota
ENOG410XQDR LUCA
GeneTreeiENSGT00530000062914
HOVERGENiHBG096249
InParanoidiA2RUC4
KOiK18066
OMAiPLYDDRP
OrthoDBiEOG091G0B98
PhylomeDBiA2RUC4
TreeFamiTF332364

Enzyme and pathway databases

UniPathwayi
UPA00375

BioCyciMetaCyc:ENSG00000162971-MONOMER
BRENDAi1.14.11.42 2681
ReactomeiR-HSA-6782861 Synthesis of wybutosine at G37 of tRNA(Phe)

Miscellaneous databases

GenomeRNAii129450
PROiPR:A2RUC4

Gene expression databases

BgeeiENSG00000162971 Expressed in 160 organ(s), highest expression level in tendon of biceps brachii
CleanExiHS_C2orf60
ExpressionAtlasiA2RUC4 baseline and differential
GenevisibleiA2RUC4 HS

Family and domain databases

InterProiView protein in InterPro
IPR003347 JmjC_dom
IPR029612 TYW5
PANTHERiPTHR12461:SF50 PTHR12461:SF50, 1 hit
SMARTiView protein in SMART
SM00558 JmjC, 1 hit
PROSITEiView protein in PROSITE
PS51184 JMJC, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTYW5_HUMAN
AccessioniPrimary (citable) accession number: A2RUC4
Secondary accession number(s): B2RNE3, Q8N1R2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: March 6, 2007
Last modified: September 12, 2018
This is version 92 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again