UniProtKB - A2RUC4 (TYW5_HUMAN)
Protein
tRNA wybutosine-synthesizing protein 5
Gene
TYW5
Organism
Homo sapiens (Human)
Status
Functioni
tRNA hydroxylase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Catalyzes the hydroxylation of 7-(a-amino-a-carboxypropyl)wyosine (yW-72) into undermodified hydroxywybutosine (OHyW*). OHyW* being further transformed into hydroxywybutosine (OHyW) by LCMT2/TYW4. OHyW is a derivative of wybutosine found in higher eukaryotes.1 Publication
Catalytic activityi
- 2-oxoglutarate + 7-[(3S)-3-amino-3-carboxypropyl]wyosine37 in tRNAPhe + O2 = 7-(2-hydroxy-3-amino-3-carboxypropyl)wyosine37 in tRNAPhe + CO2 + succinateEC:1.14.11.42
Cofactori
Fe2+1 PublicationNote: Binds 1 Fe2+ ion per subunit.1 Publication
: wybutosine-tRNA(Phe) biosynthesis Pathwayi
This protein is involved in the pathway wybutosine-tRNA(Phe) biosynthesis, which is part of tRNA modification.2 PublicationsView all proteins of this organism that are known to be involved in the pathway wybutosine-tRNA(Phe) biosynthesis and in tRNA modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 106 | 2-oxoglutarate1 Publication | 1 | |
Metal bindingi | 160 | Iron; catalytic | 1 | |
Metal bindingi | 162 | Iron; catalytic | 1 | |
Binding sitei | 166 | 2-oxoglutarate1 Publication | 1 | |
Binding sitei | 175 | 2-oxoglutarate1 Publication | 1 | |
Metal bindingi | 235 | Iron; catalytic | 1 |
GO - Molecular functioni
- 2-oxoglutarate-dependent dioxygenase activity Source: UniProtKB
- iron ion binding Source: UniProtKB
- protein homodimerization activity Source: UniProtKB
- tRNA binding Source: UniProtKB
- tRNAPhe (7-(3-amino-3-carboxypropyl)wyosine37-C2)-hydroxylase activity Source: UniProtKB-EC
GO - Biological processi
- tRNA modification Source: Reactome
- wybutosine biosynthetic process Source: UniProtKB
Keywordsi
Molecular function | Dioxygenase, Oxidoreductase |
Biological process | tRNA processing |
Ligand | Iron, Metal-binding |
Enzyme and pathway databases
BioCyci | MetaCyc:ENSG00000162971-MONOMER |
BRENDAi | 1.14.11.42, 2681 |
PathwayCommonsi | A2RUC4 |
Reactomei | R-HSA-6782861, Synthesis of wybutosine at G37 of tRNA(Phe) |
UniPathwayi | UPA00375 |
Names & Taxonomyi
Protein namesi | Recommended name: tRNA wybutosine-synthesizing protein 5 (EC:1.14.11.42)Short name: hTYW5 Alternative name(s): tRNA(Phe) (7-(3-amino-3-carboxypropyl)wyosine(37)-C(2))-hydroxylase |
Gene namesi | Name:TYW5 Synonyms:C2orf60 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:26754, TYW5 |
neXtProti | NX_A2RUC4 |
VEuPathDBi | HostDB:ENSG00000162971.10 |
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 108 | R → A: Abolishes enzyme activity and ability to bind tRNA. 1 Publication | 1 | |
Mutagenesisi | 149 | R → A: Abolishes enzyme activity and ability to bind tRNA. 1 Publication | 1 |
Organism-specific databases
OpenTargetsi | ENSG00000162971 |
PharmGKBi | PA162379298 |
Miscellaneous databases
Pharosi | A2RUC4, Tbio |
Genetic variation databases
BioMutai | TYW5 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000309274 | 1 – 315 | tRNA wybutosine-synthesizing protein 5Add BLAST | 315 |
Proteomic databases
EPDi | A2RUC4 |
jPOSTi | A2RUC4 |
MassIVEi | A2RUC4 |
MaxQBi | A2RUC4 |
PaxDbi | A2RUC4 |
PeptideAtlasi | A2RUC4 |
PRIDEi | A2RUC4 |
ProteomicsDBi | 511 [A2RUC4-1] 512 [A2RUC4-2] |
PTM databases
iPTMneti | A2RUC4 |
PhosphoSitePlusi | A2RUC4 |
Expressioni
Gene expression databases
Bgeei | ENSG00000162971, Expressed in epithelial cell of pancreas and 176 other tissues |
ExpressionAtlasi | A2RUC4, baseline and differential |
Genevisiblei | A2RUC4, HS |
Organism-specific databases
HPAi | ENSG00000162971, Low tissue specificity |
Interactioni
Subunit structurei
Homodimer.
1 PublicationGO - Molecular functioni
- protein homodimerization activity Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 126193, 11 interactors |
IntActi | A2RUC4, 8 interactors |
STRINGi | 9606.ENSP00000346627 |
Miscellaneous databases
RNActi | A2RUC4, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | A2RUC4 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 102 – 267 | JmjCPROSITE-ProRule annotationAdd BLAST | 166 |
Sequence similaritiesi
Belongs to the TYW5 family.Curated
Phylogenomic databases
eggNOGi | KOG2132, Eukaryota |
GeneTreei | ENSGT00940000158493 |
HOGENOMi | CLU_016785_4_0_1 |
InParanoidi | A2RUC4 |
OMAi | PLYDDRP |
OrthoDBi | 1385616at2759 |
PhylomeDBi | A2RUC4 |
TreeFami | TF332364 |
Family and domain databases
InterProi | View protein in InterPro IPR041667, Cupin_8 IPR003347, JmjC_dom IPR029612, TYW5 |
PANTHERi | PTHR12461:SF50, PTHR12461:SF50, 1 hit |
Pfami | View protein in Pfam PF13621, Cupin_8, 1 hit |
SMARTi | View protein in SMART SM00558, JmjC, 1 hit |
PROSITEi | View protein in PROSITE PS51184, JMJC, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: A2RUC4-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAGQHLPVPR LEGVSREQFM QHLYPQRKPL VLEGIDLGPC TSKWTVDYLS
60 70 80 90 100
QVGGKKEVKI HVAAVAQMDF ISKNFVYRTL PFDQLVQRAA EEKHKEFFVS
110 120 130 140 150
EDEKYYLRSL GEDPRKDVAD IRKQFPLLKG DIKFPEFFKE EQFFSSVFRI
160 170 180 190 200
SSPGLQLWTH YDVMDNLLIQ VTGKKRVVLF SPRDAQYLYL KGTKSEVLNI
210 220 230 240 250
DNPDLAKYPL FSKARRYECS LEAGDVLFIP ALWFHNVISE EFGVGVNIFW
260 270 280 290 300
KHLPSECYDK TDTYGNKDPT AASRAAQILD RALKTLAELP EEYRDFYARR
310
MVLHIQDKAY SKNSE
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA8KAJ9 | A8KAJ9_HUMAN | cDNA FLJ78342 | TYW5 | 121 | Annotation score: |
Sequence cautioni
The sequence AAH63502 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_036926 | 50 | S → G. Corresponds to variant dbSNP:rs10497844Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_029106 | 1 – 163 | Missing in isoform 2. 1 PublicationAdd BLAST | 163 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK095272 mRNA Translation: BAC04517.1 AK298977 mRNA Translation: BAG61071.1 AC097717 Genomic DNA Translation: AAY24162.1 CH471063 Genomic DNA Translation: EAW70191.1 BC063502 mRNA Translation: AAH63502.1 Different initiation. BC132835 mRNA Translation: AAI32836.1 BC136837 mRNA Translation: AAI36838.1 |
CCDSi | CCDS42795.1 [A2RUC4-1] |
RefSeqi | NP_001034782.1, NM_001039693.2 [A2RUC4-1] |
Genome annotation databases
Ensembli | ENST00000354611; ENSP00000346627; ENSG00000162971 [A2RUC4-1] |
GeneIDi | 129450 |
KEGGi | hsa:129450 |
UCSCi | uc002uvi.5, human [A2RUC4-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK095272 mRNA Translation: BAC04517.1 AK298977 mRNA Translation: BAG61071.1 AC097717 Genomic DNA Translation: AAY24162.1 CH471063 Genomic DNA Translation: EAW70191.1 BC063502 mRNA Translation: AAH63502.1 Different initiation. BC132835 mRNA Translation: AAI32836.1 BC136837 mRNA Translation: AAI36838.1 |
CCDSi | CCDS42795.1 [A2RUC4-1] |
RefSeqi | NP_001034782.1, NM_001039693.2 [A2RUC4-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3AL5 | X-ray | 2.50 | A/B/C/D | 1-315 | [»] | |
3AL6 | X-ray | 2.80 | A/B/C/D | 1-315 | [»] | |
SMRi | A2RUC4 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 126193, 11 interactors |
IntActi | A2RUC4, 8 interactors |
STRINGi | 9606.ENSP00000346627 |
PTM databases
iPTMneti | A2RUC4 |
PhosphoSitePlusi | A2RUC4 |
Genetic variation databases
BioMutai | TYW5 |
Proteomic databases
EPDi | A2RUC4 |
jPOSTi | A2RUC4 |
MassIVEi | A2RUC4 |
MaxQBi | A2RUC4 |
PaxDbi | A2RUC4 |
PeptideAtlasi | A2RUC4 |
PRIDEi | A2RUC4 |
ProteomicsDBi | 511 [A2RUC4-1] 512 [A2RUC4-2] |
Protocols and materials databases
Antibodypediai | 34079, 86 antibodies |
DNASUi | 129450 |
Genome annotation databases
Ensembli | ENST00000354611; ENSP00000346627; ENSG00000162971 [A2RUC4-1] |
GeneIDi | 129450 |
KEGGi | hsa:129450 |
UCSCi | uc002uvi.5, human [A2RUC4-1] |
Organism-specific databases
CTDi | 129450 |
GeneCardsi | TYW5 |
HGNCi | HGNC:26754, TYW5 |
HPAi | ENSG00000162971, Low tissue specificity |
neXtProti | NX_A2RUC4 |
OpenTargetsi | ENSG00000162971 |
PharmGKBi | PA162379298 |
VEuPathDBi | HostDB:ENSG00000162971.10 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2132, Eukaryota |
GeneTreei | ENSGT00940000158493 |
HOGENOMi | CLU_016785_4_0_1 |
InParanoidi | A2RUC4 |
OMAi | PLYDDRP |
OrthoDBi | 1385616at2759 |
PhylomeDBi | A2RUC4 |
TreeFami | TF332364 |
Enzyme and pathway databases
UniPathwayi | UPA00375 |
BioCyci | MetaCyc:ENSG00000162971-MONOMER |
BRENDAi | 1.14.11.42, 2681 |
PathwayCommonsi | A2RUC4 |
Reactomei | R-HSA-6782861, Synthesis of wybutosine at G37 of tRNA(Phe) |
Miscellaneous databases
BioGRID-ORCSi | 129450, 5 hits in 994 CRISPR screens |
GenomeRNAii | 129450 |
Pharosi | A2RUC4, Tbio |
PROi | PR:A2RUC4 |
RNActi | A2RUC4, protein |
Gene expression databases
Bgeei | ENSG00000162971, Expressed in epithelial cell of pancreas and 176 other tissues |
ExpressionAtlasi | A2RUC4, baseline and differential |
Genevisiblei | A2RUC4, HS |
Family and domain databases
InterProi | View protein in InterPro IPR041667, Cupin_8 IPR003347, JmjC_dom IPR029612, TYW5 |
PANTHERi | PTHR12461:SF50, PTHR12461:SF50, 1 hit |
Pfami | View protein in Pfam PF13621, Cupin_8, 1 hit |
SMARTi | View protein in SMART SM00558, JmjC, 1 hit |
PROSITEi | View protein in PROSITE PS51184, JMJC, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TYW5_HUMAN | |
Accessioni | A2RUC4Primary (citable) accession number: A2RUC4 Secondary accession number(s): B2RNE3, Q8N1R2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 13, 2007 |
Last sequence update: | March 6, 2007 | |
Last modified: | April 7, 2021 | |
This is version 107 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 2
Human chromosome 2: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families