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Protein

Threonine--tRNA ligase 2, cytoplasmic

Gene

TARSL2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the attachment of threonine to tRNA(Thr) in a two-step reaction: threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr). Also edits incorrectly charged tRNA(Thr) via its editing domain, at the post-transfer stage.By similarity

Catalytic activityi

ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Threonine--tRNA ligase 2, cytoplasmic (EC:6.1.1.3By similarity)
Alternative name(s):
Threonyl-tRNA synthetase
Short name:
ThrRS
Threonyl-tRNA synthetase-like protein 2
Gene namesi
Name:TARSL2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 15

Organism-specific databases

EuPathDBiHostDB:ENSG00000185418.15
HGNCiHGNC:24728 TARSL2
neXtProtiNX_A2RTX5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi123283
OpenTargetsiENSG00000185418
PharmGKBiPA128394753

Polymorphism and mutation databases

BioMutaiTARSL2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00003338282 – 802Threonine--tRNA ligase 2, cytoplasmicAdd BLAST801

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei453PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiA2RTX5
MaxQBiA2RTX5
PaxDbiA2RTX5
PRIDEiA2RTX5
ProteomicsDBi483
484 [A2RTX5-2]

PTM databases

iPTMnetiA2RTX5
PhosphoSitePlusiA2RTX5

Expressioni

Gene expression databases

BgeeiENSG00000185418 Expressed in 197 organ(s), highest expression level in corpus callosum
CleanExiHS_TARSL2
ExpressionAtlasiA2RTX5 baseline and differential
GenevisibleiA2RTX5 HS

Organism-specific databases

HPAiHPA066697

Interactioni

Subunit structurei

May be a component of the multisynthetase complex (MSC), a large multi-subunit complex which contains at least eight different aminoacyl-tRNA synthetases plus three auxillary subunits AIMP1, AIMP2 and EEF1E1. Interacts with the MSC components EPRS, AIMP1, AIMP2 and KARS.1 Publication

Binary interactionsi

Protein-protein interaction databases

BioGridi125822, 37 interactors
IntActiA2RTX5, 19 interactors
MINTiA2RTX5
STRINGi9606.ENSP00000338093

Structurei

3D structure databases

ProteinModelPortaliA2RTX5
SMRiA2RTX5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili3 – 23Sequence analysisAdd BLAST21
Coiled coili76 – 96Sequence analysisAdd BLAST21

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi786 – 792Nuclear localization signalBy similarity7

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG1637 Eukaryota
COG0441 LUCA
GeneTreeiENSGT00390000002149
HOGENOMiHOG000003878
HOVERGENiHBG059513
InParanoidiA2RTX5
KOiK01868
OMAiDAHIFML
OrthoDBiEOG091G01UL
PhylomeDBiA2RTX5
TreeFamiTF300858

Family and domain databases

CDDicd00771 ThrRS_core, 1 hit
Gene3Di3.10.20.30, 1 hit
3.40.50.800, 1 hit
HAMAPiMF_00184 Thr_tRNA_synth, 1 hit
InterProiView protein in InterPro
IPR002314 aa-tRNA-synt_IIb
IPR006195 aa-tRNA-synth_II
IPR004154 Anticodon-bd
IPR036621 Anticodon-bd_dom_sf
IPR012675 Beta-grasp_dom_sf
IPR004095 TGS
IPR012676 TGS-like
IPR002320 Thr-tRNA-ligase_IIa
IPR018163 Thr/Ala-tRNA-synth_IIc_edit
IPR033728 ThrRS_core
IPR012947 tRNA_SAD
PANTHERiPTHR11451 PTHR11451, 1 hit
PfamiView protein in Pfam
PF03129 HGTP_anticodon, 1 hit
PF02824 TGS, 1 hit
PF00587 tRNA-synt_2b, 1 hit
PF07973 tRNA_SAD, 1 hit
PRINTSiPR01047 TRNASYNTHTHR
SMARTiView protein in SMART
SM00863 tRNA_SAD, 1 hit
SUPFAMiSSF55186 SSF55186, 1 hit
SSF81271 SSF81271, 1 hit
TIGRFAMsiTIGR00418 thrS, 1 hit
PROSITEiView protein in PROSITE
PS50862 AA_TRNA_LIGASE_II, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: A2RTX5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAAEALAAEA VASRLERQEE DIRWLWSEVE RLRDEQLNAP YSCQAEGPCL
60 70 80 90 100
TREVAQLRAE NCDLRHRLCS LRLCLAEERS RQATLESAEL EAAQEAGAQP
110 120 130 140 150
PPSQSQDKDM KKKKMKESEA DSEVKHQPIF IKERLKLFEI LKKDHQLLLA
160 170 180 190 200
IYGKKGDTSN IITVRVADGQ TVQGEVWKTT PYQVAAEISQ ELAESTVIAK
210 220 230 240 250
VNGELWDLDR PLEGDSSLEL LTFDNEEAQA VYWHSSAHIL GEAMELYYGG
260 270 280 290 300
HLCYGPPIEN GFYYDMFIED RAVSSTELSA LENICKAIIK EKQPFERLEV
310 320 330 340 350
SKEILLEMFK YNKFKCRILN EKVNTATTTV YRCGPLIDLC KGPHVRHTGK
360 370 380 390 400
IKTIKIFKNS STYWEGNPEM ETLQRIYGIS FPDNKMMRDW EKFQEEAKNR
410 420 430 440 450
DHRKIGKEQE LFFFHDLSPG SCFFLPRGAF IYNTLTDFIR EEYHKRDFTE
460 470 480 490 500
VLSPNMYNSK LWEASGHWQH YSENMFTFEI EKDTFALKPM NCPGHCLMFA
510 520 530 540 550
HRPRSWREMP IRFADFGVLH RNELSGTLSG LTRVRRFQQD DAHIFCTVEQ
560 570 580 590 600
IEEEIKGCLQ FLQSVYSTFG FSFQLNLSTR PENFLGEIEM WNEAEKQLQN
610 620 630 640 650
SLMDFGEPWK MNPGDGAFYG PKIDIKIKDA IGRYHQCATI QLDFQLPIRF
660 670 680 690 700
NLTYVSKDGD DKKRPVIIHR AILGSVERMI AILSENYGGK WPFWLSPRQV
710 720 730 740 750
MVIPVGPTCE KYALQVSSEF FEEGFMADVD LDHSCTLNKK IRNAQLAQYN
760 770 780 790 800
FILVVGEKEK IDNAVNVRTR DNKIHGEILV TSAIDKLKNL RKTRTLNAEE

AF
Length:802
Mass (Da):92,646
Last modified:March 6, 2007 - v1
Checksum:iEE83EB934B503E59
GO
Isoform 2 (identifier: A2RTX5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     225-319: Missing.

Show »
Length:707
Mass (Da):81,561
Checksum:i060179AFC7232B3B
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B7ZLP8B7ZLP8_HUMAN
Probable threonine--tRNA ligase 2, ...
TARSL2
766Annotation score:
H0YKB9H0YKB9_HUMAN
Probable threonine--tRNA ligase 2, ...
TARSL2
148Annotation score:

Sequence cautioni

The sequence BAB71554 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti96A → G in BAB71241 (PubMed:14702039).Curated1
Sequence conflicti166V → A in BAB71241 (PubMed:14702039).Curated1
Sequence conflicti210R → C in BAB71241 (PubMed:14702039).Curated1
Sequence conflicti337I → N in AAH69346 (PubMed:15489334).Curated1
Sequence conflicti337I → N in AAH69811 (PubMed:15489334).Curated1
Sequence conflicti513F → C in BAB71554 (PubMed:14702039).Curated1
Sequence conflicti533R → G in BAB71554 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_033562225 – 319Missing in isoform 2. 1 PublicationAdd BLAST95

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK056653 mRNA Translation: BAB71241.1
AK057734 mRNA Translation: BAB71554.1 Different initiation.
CH471101 Genomic DNA Translation: EAX02316.1
BC069346 mRNA Translation: AAH69346.1
BC069811 mRNA Translation: AAH69811.1
BC074887 mRNA Translation: AAH74887.1
BC132671 mRNA Translation: AAI32672.1
BC136315 mRNA Translation: AAI36316.1
CCDSiCCDS10394.1 [A2RTX5-1]
RefSeqiNP_689547.2, NM_152334.2 [A2RTX5-1]
UniGeneiHs.657163

Genome annotation databases

EnsembliENST00000335968; ENSP00000338093; ENSG00000185418 [A2RTX5-1]
GeneIDi123283
KEGGihsa:123283
UCSCiuc002bxm.4 human [A2RTX5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK056653 mRNA Translation: BAB71241.1
AK057734 mRNA Translation: BAB71554.1 Different initiation.
CH471101 Genomic DNA Translation: EAX02316.1
BC069346 mRNA Translation: AAH69346.1
BC069811 mRNA Translation: AAH69811.1
BC074887 mRNA Translation: AAH74887.1
BC132671 mRNA Translation: AAI32672.1
BC136315 mRNA Translation: AAI36316.1
CCDSiCCDS10394.1 [A2RTX5-1]
RefSeqiNP_689547.2, NM_152334.2 [A2RTX5-1]
UniGeneiHs.657163

3D structure databases

ProteinModelPortaliA2RTX5
SMRiA2RTX5
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi125822, 37 interactors
IntActiA2RTX5, 19 interactors
MINTiA2RTX5
STRINGi9606.ENSP00000338093

PTM databases

iPTMnetiA2RTX5
PhosphoSitePlusiA2RTX5

Polymorphism and mutation databases

BioMutaiTARSL2

Proteomic databases

EPDiA2RTX5
MaxQBiA2RTX5
PaxDbiA2RTX5
PRIDEiA2RTX5
ProteomicsDBi483
484 [A2RTX5-2]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000335968; ENSP00000338093; ENSG00000185418 [A2RTX5-1]
GeneIDi123283
KEGGihsa:123283
UCSCiuc002bxm.4 human [A2RTX5-1]

Organism-specific databases

CTDi123283
DisGeNETi123283
EuPathDBiHostDB:ENSG00000185418.15
GeneCardsiTARSL2
HGNCiHGNC:24728 TARSL2
HPAiHPA066697
neXtProtiNX_A2RTX5
OpenTargetsiENSG00000185418
PharmGKBiPA128394753
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1637 Eukaryota
COG0441 LUCA
GeneTreeiENSGT00390000002149
HOGENOMiHOG000003878
HOVERGENiHBG059513
InParanoidiA2RTX5
KOiK01868
OMAiDAHIFML
OrthoDBiEOG091G01UL
PhylomeDBiA2RTX5
TreeFamiTF300858

Miscellaneous databases

ChiTaRSiTARSL2 human
GenomeRNAii123283
PROiPR:A2RTX5

Gene expression databases

BgeeiENSG00000185418 Expressed in 197 organ(s), highest expression level in corpus callosum
CleanExiHS_TARSL2
ExpressionAtlasiA2RTX5 baseline and differential
GenevisibleiA2RTX5 HS

Family and domain databases

CDDicd00771 ThrRS_core, 1 hit
Gene3Di3.10.20.30, 1 hit
3.40.50.800, 1 hit
HAMAPiMF_00184 Thr_tRNA_synth, 1 hit
InterProiView protein in InterPro
IPR002314 aa-tRNA-synt_IIb
IPR006195 aa-tRNA-synth_II
IPR004154 Anticodon-bd
IPR036621 Anticodon-bd_dom_sf
IPR012675 Beta-grasp_dom_sf
IPR004095 TGS
IPR012676 TGS-like
IPR002320 Thr-tRNA-ligase_IIa
IPR018163 Thr/Ala-tRNA-synth_IIc_edit
IPR033728 ThrRS_core
IPR012947 tRNA_SAD
PANTHERiPTHR11451 PTHR11451, 1 hit
PfamiView protein in Pfam
PF03129 HGTP_anticodon, 1 hit
PF02824 TGS, 1 hit
PF00587 tRNA-synt_2b, 1 hit
PF07973 tRNA_SAD, 1 hit
PRINTSiPR01047 TRNASYNTHTHR
SMARTiView protein in SMART
SM00863 tRNA_SAD, 1 hit
SUPFAMiSSF55186 SSF55186, 1 hit
SSF81271 SSF81271, 1 hit
TIGRFAMsiTIGR00418 thrS, 1 hit
PROSITEiView protein in PROSITE
PS50862 AA_TRNA_LIGASE_II, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSYTC2_HUMAN
AccessioniPrimary (citable) accession number: A2RTX5
Secondary accession number(s): B2RMP7
, Q6B0A1, Q6IS76, Q96LW3, Q96MP4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: March 6, 2007
Last modified: November 7, 2018
This is version 112 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  3. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
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