UniProtKB - A2RAF3 (DCL1_ASPNC)
Protein
Dicer-like protein 1
Gene
dcl1
Organism
Aspergillus niger (strain CBS 513.88 / FGSC A1513)
Status
Functioni
Dicer-like endonuclease involved in cleaving double-stranded RNA in the RNA interference (RNAi) pathway. Produces 21 to 25 bp dsRNAs (siRNAs) which target the selective destruction of homologous RNAs leading to sequence-specific suppression of gene expression, called post-transcriptional gene silencing (PTGS). Part of a broad host defense response against viral infection and transposons (By similarity).By similarity
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 1283 | Magnesium or manganeseBy similarity | 1 | |
Sitei | 1376 | Important for activityBy similarity | 1 | |
Metal bindingi | 1380 | Magnesium or manganeseBy similarity | 1 | |
Metal bindingi | 1383 | Magnesium or manganeseBy similarity | 1 | |
Metal bindingi | 1440 | ZincBy similarity | 1 | |
Metal bindingi | 1467 | Zinc; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 1508 | ZincBy similarity | 1 | |
Metal bindingi | 1510 | ZincBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 137 – 144 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- DNA binding Source: InterPro
- helicase activity Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- ribonuclease III activity Source: InterPro
- RNA binding Source: UniProtKB-KW
GO - Biological processi
- defense response to virus Source: UniProtKB-KW
- regulation of defense response to virus Source: UniProtKB-KW
- RNA processing Source: InterPro
Keywordsi
Molecular function | Antiviral protein, Helicase, Hydrolase, RNA-binding |
Biological process | Antiviral defense |
Ligand | ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, Zinc |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:dcl1 ORF Names:An18g02950 |
Organismi | Aspergillus niger (strain CBS 513.88 / FGSC A1513) |
Taxonomic identifieri | 425011 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Aspergillaceae › Aspergillus › Aspergillus subgen. Circumdati › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000306775 | 1 – 1525 | Dicer-like protein 1Add BLAST | 1525 |
Proteomic databases
PaxDbi | A2RAF3 |
PRIDEi | A2RAF3 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 124 – 305 | Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST | 182 | |
Domaini | 439 – 605 | Helicase C-terminalPROSITE-ProRule annotationAdd BLAST | 167 | |
Domaini | 641 – 731 | Dicer dsRNA-binding foldPROSITE-ProRule annotationAdd BLAST | 91 | |
Domaini | 1032 – 1192 | RNase III 1PROSITE-ProRule annotationAdd BLAST | 161 | |
Domaini | 1243 – 1394 | RNase III 2PROSITE-ProRule annotationAdd BLAST | 152 | |
Domaini | 1428 – 1496 | DRBMAdd BLAST | 69 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 250 – 253 | DEAH box | 4 |
Sequence similaritiesi
Keywords - Domaini
RepeatFamily and domain databases
CDDi | cd00593, RIBOc, 2 hits |
Gene3Di | 1.10.1520.10, 2 hits 3.30.160.380, 1 hit |
InterProi | View protein in InterPro IPR038248, Dicer_dimer_sf IPR005034, Dicer_dimerisation_dom IPR006935, Helicase/UvrB_N IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR000999, RNase_III_dom IPR036389, RNase_III_sf |
Pfami | View protein in Pfam PF00271, Helicase_C, 1 hit PF04851, ResIII, 1 hit PF00636, Ribonuclease_3, 2 hits |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit SM00535, RIBOc, 2 hits |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF69065, SSF69065, 2 hits |
PROSITEi | View protein in PROSITE PS51327, DICER_DSRBF, 1 hit PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS00517, RNASE_3_1, 1 hit PS50142, RNASE_3_2, 2 hits |
i Sequence
Sequence statusi: Complete.
A2RAF3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPSAHAFDMR ITSDIYKPDQ HLPMETMKVC AVTMTEDLQE DDGSSDESDN
60 70 80 90 100
DEREDHSKTG VSQQRITQNA KFKALLAQRA DTGPIHDVSV THDLPDAQLS
110 120 130 140 150
TAHLVAKQDL GIGTLDPREY QLELFERAKV QNTIAVLDTG SGKTLIAVLL
160 170 180 190 200
LKHTLEKELN DRMEGKPHRI AFFLVDSVTL AYQQAAVLRN NLDQSVGHFF
210 220 230 240 250
GAMGTDLWSK SVWDQHFQKN MVIVCTAEIL NQCLLNSYIK MSQINILIFD
260 270 280 290 300
EAHHTKKDHP YARIIRDSYL EEVYSKRPRI FGMTASPIDT KGDIVDEATR
310 320 330 340 350
LEKLLDSRIA TTSNMSLLRQ VARRPVERVW SFNKLEQPFA TSLYKHLEDR
360 370 380 390 400
FGDMACLEGI FRFAWQASSE LGRWCSDRAW ARALADDVLP KLEGSVRKTA
410 420 430 440 450
NSETSSNVPE SAYKEILRIT EASEIVKSYA FSSPETFGQL SPKVQVLREE
460 470 480 490 500
LARYFGRQTE TKCIVFTQKR YTALILAELF QTLNIPFLRP GVLIGVRSGD
510 520 530 540 550
LAGMNITFRQ QFISLVKFRT GEINCLFATS VAEEGLDIPD CNLVVRFDLY
560 570 580 590 600
QTLIQYVQSR GRARHFNSTY ASMVERGNLE HEQRLLEVQD AEMMQSFCRT
610 620 630 640 650
LPEDRLLYGF DHDLDTVLQK DEGNRTFRIK STGAKLTYHS ATAILARYAS
660 670 680 690 700
SLQYEKEFSA QVTYVVLPIN GAFVCEVILP EKSPIRGLTG SPAMKKSIAK
710 720 730 740 750
RSAAFDTCLL LRKNKLLDDH FNSIYHRRLP AMRNAKLAIT SKRTSQYDMI
760 770 780 790 800
SKPSLWGRKQ GMPPKELHGT FITFLPSMQL SHEPAPLLLF TRERLPHFPE
810 820 830 840 850
FPIFLDDDVE TTIITTPLEK QLLLSEKEVD ALTVFTLRVF RDVFHKTYDK
860 870 880 890 900
EPEKMAYWLA PAKVQSSYLP SYDPRQILDW ESLTYVRDND SIPFSTNADP
910 920 930 940 950
ESWVDLFVFD AWDGRCRFFT VGVEHSLTPS SPPPPFVARR RHMNDVMNYC
960 970 980 990 1000
LSLSKNSRAK FLSTCHWDQP VLRAELVRLR RNLLDKMTDT ERDVETRCFI
1010 1020 1030 1040 1050
CIEPLKVSAI PASTAFSCLA FPAIISRIDA YLISLQGCES LNFTVKLDLA
1060 1070 1080 1090 1100
LEAFTKDSDN TDEHRAQQIH VQRGMGRNYE RLEFLGDCFL KMATSIALFT
1110 1120 1130 1140 1150
QNPDDDEFDY HVNRMCLICN KNLFNAAVDK EIYKYIRSRG FSRHTWYPEG
1160 1170 1180 1190 1200
LKLLQGKDHS RKATTESKHA LAEKTIADVC EALIGAALLS GGPDHRFDMA
1210 1220 1230 1240 1250
VKAVTTLVNS PSHKAERWKD YISFYTIPKY QRRAADGAEL YLSRKIEEKL
1260 1270 1280 1290 1300
SYRFRYPTLL GSAFTHPSYP SAWAKVPCYQ RLEFLGDSLI DMVCVEDLFA
1310 1320 1330 1340 1350
RFPDRDPQWL TEHKMAMVSN KFLGALAVKL GLHTHLKYFS APLQSQITQY
1360 1370 1380 1390 1400
AEEIQTAEGE SEGAVDYWTV TKDPPKCLPD MVEAYVGAVF VDSDFNFEVI
1410 1420 1430 1440 1450
ERFFRDYIKP FFEDMAIYDT FANKHPTTFL HNRLTNEFGC VNYCLKAGEM
1460 1470 1480 1490 1500
PSIDGAPAGV LAAVIVHDVV IAEGTATSGR YAKVKASEKA LAVLDEISSA
1510 1520
EFQRKFRCDC RESGDSARLD IGTAI
Sequence cautioni
The sequence CAK48679 differs from that shown. Reason: Erroneous gene model prediction.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AM270401 Genomic DNA Translation: CAK48679.1 Sequence problems. |
Genome annotation databases
EnsemblFungii | CAK48679; CAK48679; An18g02950 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AM270401 Genomic DNA Translation: CAK48679.1 Sequence problems. |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Proteomic databases
PaxDbi | A2RAF3 |
PRIDEi | A2RAF3 |
Genome annotation databases
EnsemblFungii | CAK48679; CAK48679; An18g02950 |
Family and domain databases
CDDi | cd00593, RIBOc, 2 hits |
Gene3Di | 1.10.1520.10, 2 hits 3.30.160.380, 1 hit |
InterProi | View protein in InterPro IPR038248, Dicer_dimer_sf IPR005034, Dicer_dimerisation_dom IPR006935, Helicase/UvrB_N IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR000999, RNase_III_dom IPR036389, RNase_III_sf |
Pfami | View protein in Pfam PF00271, Helicase_C, 1 hit PF04851, ResIII, 1 hit PF00636, Ribonuclease_3, 2 hits |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit SM00535, RIBOc, 2 hits |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF69065, SSF69065, 2 hits |
PROSITEi | View protein in PROSITE PS51327, DICER_DSRBF, 1 hit PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS00517, RNASE_3_1, 1 hit PS50142, RNASE_3_2, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DCL1_ASPNC | |
Accessioni | A2RAF3Primary (citable) accession number: A2RAF3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 2, 2007 |
Last sequence update: | October 2, 2007 | |
Last modified: | October 7, 2020 | |
This is version 85 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families