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Protein

NADPH--cytochrome P450 reductase

Gene

cprA

Organism
Aspergillus niger (strain CBS 513.88 / FGSC A1513)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. Involved in ergosterol biosynthesis.UniRule annotation

Catalytic activityi

NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.UniRule annotation

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei204FMNUniRule annotation1
Binding sitei296NADPUniRule annotation1
Binding sitei552NADPUniRule annotation1
Binding sitei656NADPUniRule annotation1
Binding sitei694FADUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi72 – 77FMNUniRule annotation6
Nucleotide bindingi123 – 126FMNUniRule annotation4
Nucleotide bindingi169 – 178FMNUniRule annotation10
Nucleotide bindingi451 – 454FADUniRule annotation4
Nucleotide bindingi469 – 471FADUniRule annotation3
Nucleotide bindingi486 – 489FADUniRule annotation4
Nucleotide bindingi614 – 615NADPUniRule annotation2
Nucleotide bindingi620 – 624NADPUniRule annotation5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processLipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism
LigandFAD, Flavoprotein, FMN, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH--cytochrome P450 reductaseUniRule annotation (EC:1.6.2.4UniRule annotation)
Short name:
CPRUniRule annotation
Short name:
P450RUniRule annotation
Gene namesi
Name:cprAUniRule annotation
ORF Names:An08g07840
OrganismiAspergillus niger (strain CBS 513.88 / FGSC A1513)
Taxonomic identifieri425011 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000006706 Componenti: Chromosome 8R

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 8LumenalUniRule annotation8
Transmembranei9 – 31HelicalUniRule annotationAdd BLAST23
Topological domaini32 – 695CytoplasmicUniRule annotationAdd BLAST664

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_50002203241 – 695NADPH--cytochrome P450 reductaseAdd BLAST695

Proteomic databases

PaxDbiA2QS05

Interactioni

Protein-protein interaction databases

STRINGi5061.CADANGAP00006979

Structurei

3D structure databases

ProteinModelPortaliA2QS05
SMRiA2QS05
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini66 – 221Flavodoxin-likeUniRule annotationAdd BLAST156
Domaini277 – 538FAD-binding FR-typeUniRule annotationAdd BLAST262

Sequence similaritiesi

Belongs to the NADPH--cytochrome P450 reductase family.UniRule annotation
In the N-terminal section; belongs to the flavodoxin family.UniRule annotation
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000282027
KOiK00327
OrthoDBiEOG092C1RRH

Family and domain databases

Gene3Di1.20.990.10, 1 hit
3.40.50.360, 1 hit
3.40.50.80, 1 hit
HAMAPiMF_03212 NCPR, 1 hit
InterProiView protein in InterPro
IPR003097 CysJ-like_FAD-binding
IPR017927 FAD-bd_FR_type
IPR001094 Flavdoxin-like
IPR008254 Flavodoxin/NO_synth
IPR001709 Flavoprot_Pyr_Nucl_cyt_Rdtase
IPR029039 Flavoprotein-like_sf
IPR039261 FNR_nucleotide-bd
IPR023173 NADPH_Cyt_P450_Rdtase_alpha
IPR001433 OxRdtase_FAD/NAD-bd
IPR023208 P450R
IPR017938 Riboflavin_synthase-like_b-brl
PfamiView protein in Pfam
PF00667 FAD_binding_1, 1 hit
PF00258 Flavodoxin_1, 1 hit
PF00175 NAD_binding_1, 1 hit
PIRSFiPIRSF000208 P450R, 1 hit
PRINTSiPR00369 FLAVODOXIN
PR00371 FPNCR
SUPFAMiSSF52218 SSF52218, 1 hit
SSF52343 SSF52343, 1 hit
SSF63380 SSF63380, 1 hit
PROSITEiView protein in PROSITE
PS51384 FAD_FR, 1 hit
PS50902 FLAVODOXIN_LIKE, 1 hit

Sequencei

Sequence statusi: Complete.

A2QS05-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAQLDTLDLV VLAVLLVGSV AYFTKGTYWA VAKDPYASTG PAMNGAAKAG
60 70 80 90 100
KTRNIIEKME ETGKNCVIFY GSQTGTAEDY ASRLAKEGSQ RFGLKTMVAD
110 120 130 140 150
LEEYDYENLD QFPEDKVAFF VLATYGEGEP TDNAVEFYQF FTGDDVAFES
160 170 180 190 200
GASADEKPLS KLKYVAFGLG NNTYEHYNAM VRQVDAAFQK LGAQRIGSAG
210 220 230 240 250
EGDDGAGTME EDFLAWKEPM WAALSESMDL QEREAVYEPV FCVTENESLS
260 270 280 290 300
PEDETVYLGE PTQSHLQGTP KGPYSAHNPF IAPIAESREL FTVKDRNCLH
310 320 330 340 350
MEISIAGSNL SYQTGDHIAV WPTNAGAEVD RFLQVFGLEG KRDSVINIKG
360 370 380 390 400
IDVTAKVPIP TPTTYDAAVR YYMEVCAPVS RQFVATLAAF APDEESKAEI
410 420 430 440 450
VRLGSDKDYF HEKVTNQCFN IAQALQSITS KPFSAVPFSL LIEGITKLQP
460 470 480 490 500
RYYSISSSSL VQKDKISITA VVESVRLPGA SHMVKGVTTN YLLALKQKQN
510 520 530 540 550
GDPSPDPHGL TYSITGPRNK YDGIHVPVHV RHSNFKLPSD PSRPIIMVGP
560 570 580 590 600
GTGVAPFRGF IQERAALAAK GEKVGPTVLF FGCRKSDEDF LYKDEWKTYQ
610 620 630 640 650
DQLGDNLKII TAFSREGPQK VYVQHRLREH SELVSDLLKQ KATFYVCGDA
660 670 680 690
ANMAREVNLV LGQIIAAQRG LPAEKGEEMV KHMRSSGSYQ EDVWS
Length:695
Mass (Da):76,589
Last modified:March 6, 2007 - v1
Checksum:i835324930A8A62F3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM270176 Genomic DNA Translation: CAK45677.1
RefSeqiXP_001392901.1, XM_001392864.2

Genome annotation databases

GeneIDi4983103
KEGGiang:ANI_1_1104074

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM270176 Genomic DNA Translation: CAK45677.1
RefSeqiXP_001392901.1, XM_001392864.2

3D structure databases

ProteinModelPortaliA2QS05
SMRiA2QS05
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi5061.CADANGAP00006979

Proteomic databases

PaxDbiA2QS05

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi4983103
KEGGiang:ANI_1_1104074

Phylogenomic databases

HOGENOMiHOG000282027
KOiK00327
OrthoDBiEOG092C1RRH

Family and domain databases

Gene3Di1.20.990.10, 1 hit
3.40.50.360, 1 hit
3.40.50.80, 1 hit
HAMAPiMF_03212 NCPR, 1 hit
InterProiView protein in InterPro
IPR003097 CysJ-like_FAD-binding
IPR017927 FAD-bd_FR_type
IPR001094 Flavdoxin-like
IPR008254 Flavodoxin/NO_synth
IPR001709 Flavoprot_Pyr_Nucl_cyt_Rdtase
IPR029039 Flavoprotein-like_sf
IPR039261 FNR_nucleotide-bd
IPR023173 NADPH_Cyt_P450_Rdtase_alpha
IPR001433 OxRdtase_FAD/NAD-bd
IPR023208 P450R
IPR017938 Riboflavin_synthase-like_b-brl
PfamiView protein in Pfam
PF00667 FAD_binding_1, 1 hit
PF00258 Flavodoxin_1, 1 hit
PF00175 NAD_binding_1, 1 hit
PIRSFiPIRSF000208 P450R, 1 hit
PRINTSiPR00369 FLAVODOXIN
PR00371 FPNCR
SUPFAMiSSF52218 SSF52218, 1 hit
SSF52343 SSF52343, 1 hit
SSF63380 SSF63380, 1 hit
PROSITEiView protein in PROSITE
PS51384 FAD_FR, 1 hit
PS50902 FLAVODOXIN_LIKE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNCPR_ASPNC
AccessioniPrimary (citable) accession number: A2QS05
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 8, 2011
Last sequence update: March 6, 2007
Last modified: October 10, 2018
This is version 75 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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