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Protein

AT-rich interactive domain-containing protein 4B

Gene

Arid4b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a transcriptional repressor. May function in the assembly and/or enzymatic activity of the Sin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes (By similarity). Plays a role in the regulation of epigenetic modifications at the PWS/AS imprinting center near the SNRPN promoter, where it might function as part of a complex with RB1 and ARID4A (PubMed:17043311). Involved in spermatogenesis, together with ARID4A, where it functions as a transcriptional coactivator for AR (androgen receptor) and enhances expression of genes required for sperm maturation. Regulates expression of the tight junction protein CLDN3 in the testis, which is important for integrity of the blood-testis barrier (PubMed:23487765). Plays a role in myeloid homeostasis where it regulates the histone methylation state of bone marrow cells and expression of various genes involved in hematopoiesis. May function as a leukemia suppressor (PubMed:18728284).By similarity3 Publications

GO - Molecular functioni

  • transcription regulatory region DNA binding Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionChromatin regulator, DNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-3214815 HDACs deacetylate histones

Names & Taxonomyi

Protein namesi
Recommended name:
AT-rich interactive domain-containing protein 4B
Short name:
ARID domain-containing protein 4B
Alternative name(s):
180 kDa Sin3-associated polypeptide
Short name:
Sin3-associated polypeptide p180
Histone deacetylase complex subunit SAP180
Gene namesi
Name:Arid4b
Synonyms:Sap180
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:2137512 Arid4b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Embryonic lethal. Lethality occurs between embryonic day E3.5 and E7.5 (PubMed:17043311). Heterozygous males are fully fertile (PubMed:23487765). Double knockouts of ARID4B heterozygotes with ARID4A homozygotes show various hematological abnormalities, which are more severe than the ARID4A phenotype alone. Hematopoietic stem cell (HSC) and common myeloid progenitor (CMP) counts in bone marrow and spleen are increased. Lymphocyte numbers in spleen are significantly reduced. In peripheral blood, red blood cell counts and lymphocyte counts are reduced. Approximately 83% of animals develop acute myeloid leukemia (AML) and/or myeloid sarcoma. In bone marrow cells, expression of FOXP3 is significantly reduced (PubMed:18728284). Expression of HOXB3, HOXB5, HOXB6, HOXB8 and PITX2 in bone marrow cells is reduced (PubMed:17043311). Males show progressive reduction in fertility from 2 months of age onwards, with reduced testis size and variable defects in seminal vesicle formation. Spermatogenesis is partially blocked from the meiosis II stage onwards leading to reduced numbers of mature spermatozoa. Expression in testis of CLDN3, an androgen receptor-regulated gene, is significantly reduced. Expression of PTGDS is also reduced, whereas expression of INHA and EMB is moderately increased (PubMed:23487765).3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002828641 – 1314AT-rich interactive domain-containing protein 4BAdd BLAST1314

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei276PhosphoserineBy similarity1
Modified residuei295PhosphoserineBy similarity1
Modified residuei296PhosphoserineBy similarity1
Cross-linki428Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki461Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei482PhosphoserineBy similarity1
Modified residuei666PhosphoserineBy similarity1
Modified residuei668PhosphoserineCombined sources1
Modified residuei675PhosphoserineCombined sources1
Modified residuei717PhosphoserineBy similarity1
Cross-linki751Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei778PhosphoserineBy similarity1
Modified residuei790PhosphoserineCombined sources1
Modified residuei793PhosphothreonineCombined sources1
Modified residuei1016PhosphoserineBy similarity1
Modified residuei1028PhosphothreonineBy similarity1
Modified residuei1031PhosphoserineBy similarity1
Modified residuei1152PhosphothreonineBy similarity1
Modified residuei1154PhosphoserineBy similarity1
Modified residuei1155PhosphoserineBy similarity1
Modified residuei1157PhosphoserineBy similarity1
Modified residuei1161PhosphoserineBy similarity1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiA2CG63
MaxQBiA2CG63
PaxDbiA2CG63
PeptideAtlasiA2CG63
PRIDEiA2CG63

PTM databases

iPTMnetiA2CG63
PhosphoSitePlusiA2CG63

Expressioni

Tissue specificityi

Expressed in Sertoli cells of the testis.1 Publication

Gene expression databases

BgeeiENSMUSG00000039219 Expressed in 270 organ(s), highest expression level in supraoptic nucleus
CleanExiMM_ARID4B
ExpressionAtlasiA2CG63 baseline and differential
GenevisibleiA2CG63 MM

Interactioni

Subunit structurei

Component of a Sin3A corepressor complex consisting of SIN3A, SAP130, SUDS3/SAP45, SAP180, HDAC1 and HDAC2 (By similarity). Interacts with ARID4A (PubMed:17043311). Interacts with AR (PubMed:23487765).By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Stra8P702784EBI-6394082,EBI-6394115

Protein-protein interaction databases

BioGridi220492, 3 interactors
ComplexPortaliCPX-3441 SIN3A histone deacetylase complex, ES cell-specific variant
CPX-3443 SIN3A histone deacetylase complex
CPX-3444 SIN3B histone deacetylase complex
IntActiA2CG63, 1 interactor
STRINGi10090.ENSMUSP00000106163

Structurei

3D structure databases

ProteinModelPortaliA2CG63
SMRiA2CG63
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini306 – 398ARIDPROSITE-ProRule annotationAdd BLAST93

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili1227 – 1272Sequence analysisAdd BLAST46

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi154 – 159Poly-Ser6
Compositional biasi268 – 567Glu-richAdd BLAST300
Compositional biasi1097 – 1103Poly-Ser7
Compositional biasi1273 – 1305Ser-richAdd BLAST33

Domaini

The C-terminus mediates interaction with mSin3A corepressor complex.By similarity
The N-terminus is involved in transcriptional repression by HDAC-independent mechanisms.By similarity
The ARID domain is involved in stabilizing the mSin3A corepressor complex on DNA.By similarity

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2744 Eukaryota
KOG3001 Eukaryota
ENOG410Y2AP LUCA
GeneTreeiENSGT00900000140901
HOVERGENiHBG058247
InParanoidiA2CG63
KOiK19195
OMAiTHTRNDL
OrthoDBiEOG091G0EG4
PhylomeDBiA2CG63
TreeFamiTF106427

Family and domain databases

CDDicd00024 CHROMO, 1 hit
Gene3Di1.10.150.60, 1 hit
InterProiView protein in InterPro
IPR028853 ARID4B
IPR001606 ARID_dom
IPR036431 ARID_dom_sf
IPR016197 Chromo-like_dom_sf
IPR000953 Chromo/chromo_shadow_dom
IPR012603 RBB1NT
IPR002999 Tudor
IPR025995 Tudor-knot
PANTHERiPTHR13964:SF24 PTHR13964:SF24, 1 hit
PfamiView protein in Pfam
PF01388 ARID, 1 hit
PF08169 RBB1NT, 1 hit
PF11717 Tudor-knot, 1 hit
SMARTiView protein in SMART
SM00501 BRIGHT, 1 hit
SM00333 TUDOR, 2 hits
SUPFAMiSSF46774 SSF46774, 1 hit
SSF54160 SSF54160, 1 hit
PROSITEiView protein in PROSITE
PS51011 ARID, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: A2CG63-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MKALDEPPYL TVGTDVSAKY RGAFCEAKIK TAKRLVKVKV TFRHDSSTVE
60 70 80 90 100
VQDDHIKGPL KVGAIVEVKN LDGAYQEAVI NKLTDASWYT VVFDDGDEKT
110 120 130 140 150
LRRSSLCLKG ERHFAESETL DQLPLTNPEH FGTPVIGKKT NRGRRSNHIP
160 170 180 190 200
EEESSSSSSD DDEEERKQTD ELLGKVVCVD YVSLEKKKAM WFPALVVCPD
210 220 230 240 250
CSDEIAVKKD NILVRSFKDG KFTSVPRKDV HEITSDTVPK PDAVLKQAFD
260 270 280 290 300
QALEFHKSRA IPANWKTELK EDSSSSEAEE EEEEEDDEKE KEDNSSEEEE
310 320 330 340 350
EIEPFPEERE NFLQQLYKFM EDRGTPINKR PVLGYRNLNL FKLFRLVHKL
360 370 380 390 400
GGFDNIESGA VWKQVYQDLG IPVLNSAAGY NVKCAYKKYL YGFEEYCRSA
410 420 430 440 450
NIDFQMALPE KVLNKPCKDC ENKEVKVKEE SETEIKEVNV EDSKNVMPKE
460 470 480 490 500
ETPAEDESER KENIKPSLGS KKSLLECIPA QSDEEKEAHI TKLEENENLE
510 520 530 540 550
DKDGGRARTE EAFSTEVDGE EEQARSGDET NKEEDEDDEE IEEEEEEDEE
560 570 580 590 600
EDEDEDDDDN NEEEEFECYP PGMKVQVRYG RGKNQKMYEA SIKDSDVEGG
610 620 630 640 650
EALYLVHYCG WNVRYDEWIK ADKIVRPADK NVPKIKHRKK IKNKLDKEKD
660 670 680 690 700
RDEKYSPKNC KLRRLSKSPF QSNPSPEMVS KLDLADAKNS DTAHIKSIEI
710 720 730 740 750
TSILNGLQAS ESSAEDSEQE DERCTQDVDN IGKDESKVEH STHSRNELIS
760 770 780 790 800
KEEQSSPSLL EENKVHTDLV IAKTVSKSPE RLRKDMEAIS EDTDFEEEDE
810 820 830 840 850
ITKKRKDVKK DTTDKALKPQ TKRGKRRYCS ADECLQTGSP GKKEDRTKSK
860 870 880 890 900
EPLCTENSSN SSSDEDEEEK SKAKMTPTKK YNGLEEKRKS LRTTSFYSGF
910 920 930 940 950
SEVAEKRIKL LNNSDERLQN NRAKDRKDVW SSIQGQWPKK TLKELFSDSD
960 970 980 990 1000
TEAAASPPHP APDEGAVEES LQTVAEEESC SPIMELEKPL PASVDNKPIE
1010 1020 1030 1040 1050
EKPLEVSDRK TEFPSSGSNS VLNTPPTTPE SPSSVTITEA SQQQSSVTVS
1060 1070 1080 1090 1100
VPLPPNQEEV RSIKSETDST IEVDSVVGEL QDLQSEGNSS PAGFDASVSS
1110 1120 1130 1140 1150
SSSNQPEPDN PEKACTGQKR VKDTQGVGSS SKKQKRSHKA TVVNNKKKGK
1160 1170 1180 1190 1200
GTNSSDSEEL SAGESVTKTQ TIKSVPTGMK THNSKSPARV QSPGKGGRNG
1210 1220 1230 1240 1250
DKDPDLKEPS NRLPKVYKWS FQTSDLENMT SAERISILQE KLQEIRKHYL
1260 1270 1280 1290 1300
SLKSEVASID RRRKRLKKKE RESAATSSSS SSPSSSSITA AVMLTLAEPS
1310
MSSASQNGMS VECR
Length:1,314
Mass (Da):147,643
Last modified:February 20, 2007 - v1
Checksum:iDE5E69DAA208A200
GO
Isoform 2 (identifier: A2CG63-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     528-614: Missing.

Show »
Length:1,227
Mass (Da):137,325
Checksum:i28B18F3DB2D6884D
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Z4YL44Z4YL44_MOUSE
AT-rich interactive domain-containi...
Arid4b
473Annotation score:
Z4YMH1Z4YMH1_MOUSE
AT-rich interactive domain-containi...
Arid4b
540Annotation score:
A0A1Y7VIX3A0A1Y7VIX3_MOUSE
AT rich interactive domain 4B (Rbp1...
Arid4b mCG_18829
128Annotation score:
A0A1Y7VKA9A0A1Y7VKA9_MOUSE
AT-rich interactive domain-containi...
Arid4b
31Annotation score:

Sequence cautioni

The sequence CAM21242 differs from that shown. Reason: Erroneous gene model prediction.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_024236528 – 614Missing in isoform 2. CuratedAdd BLAST87

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CT025604 Genomic DNA Translation: CAM21242.1 Sequence problems.
CT025604 Genomic DNA Translation: CAM21243.1
CT025604 Genomic DNA Translation: CAM21244.1
CT025604 Genomic DNA Translation: CAM21245.1
BC137783 mRNA Translation: AAI37784.1
AK031062 mRNA Translation: BAC27233.1
AK041414 mRNA Translation: BAC30936.1
CCDSiCCDS36599.1 [A2CG63-1]
CCDS36600.1 [A2CG63-2]
RefSeqiNP_919238.1, NM_194262.2 [A2CG63-1]
NP_937755.1, NM_198122.2 [A2CG63-2]
XP_006516869.1, XM_006516806.2 [A2CG63-1]
XP_006516870.1, XM_006516807.2 [A2CG63-1]
XP_017171137.1, XM_017315648.1 [A2CG63-1]
UniGeneiMm.439784

Genome annotation databases

EnsembliENSMUST00000039538; ENSMUSP00000043889; ENSMUSG00000039219 [A2CG63-2]
ENSMUST00000110534; ENSMUSP00000106163; ENSMUSG00000039219 [A2CG63-1]
ENSMUST00000110536; ENSMUSP00000106165; ENSMUSG00000039219 [A2CG63-2]
GeneIDi94246
KEGGimmu:94246
UCSCiuc007pmz.2 mouse [A2CG63-1]
uc007pna.2 mouse [A2CG63-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CT025604 Genomic DNA Translation: CAM21242.1 Sequence problems.
CT025604 Genomic DNA Translation: CAM21243.1
CT025604 Genomic DNA Translation: CAM21244.1
CT025604 Genomic DNA Translation: CAM21245.1
BC137783 mRNA Translation: AAI37784.1
AK031062 mRNA Translation: BAC27233.1
AK041414 mRNA Translation: BAC30936.1
CCDSiCCDS36599.1 [A2CG63-1]
CCDS36600.1 [A2CG63-2]
RefSeqiNP_919238.1, NM_194262.2 [A2CG63-1]
NP_937755.1, NM_198122.2 [A2CG63-2]
XP_006516869.1, XM_006516806.2 [A2CG63-1]
XP_006516870.1, XM_006516807.2 [A2CG63-1]
XP_017171137.1, XM_017315648.1 [A2CG63-1]
UniGeneiMm.439784

3D structure databases

ProteinModelPortaliA2CG63
SMRiA2CG63
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi220492, 3 interactors
ComplexPortaliCPX-3441 SIN3A histone deacetylase complex, ES cell-specific variant
CPX-3443 SIN3A histone deacetylase complex
CPX-3444 SIN3B histone deacetylase complex
IntActiA2CG63, 1 interactor
STRINGi10090.ENSMUSP00000106163

PTM databases

iPTMnetiA2CG63
PhosphoSitePlusiA2CG63

Proteomic databases

EPDiA2CG63
MaxQBiA2CG63
PaxDbiA2CG63
PeptideAtlasiA2CG63
PRIDEiA2CG63

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000039538; ENSMUSP00000043889; ENSMUSG00000039219 [A2CG63-2]
ENSMUST00000110534; ENSMUSP00000106163; ENSMUSG00000039219 [A2CG63-1]
ENSMUST00000110536; ENSMUSP00000106165; ENSMUSG00000039219 [A2CG63-2]
GeneIDi94246
KEGGimmu:94246
UCSCiuc007pmz.2 mouse [A2CG63-1]
uc007pna.2 mouse [A2CG63-2]

Organism-specific databases

CTDi51742
MGIiMGI:2137512 Arid4b

Phylogenomic databases

eggNOGiKOG2744 Eukaryota
KOG3001 Eukaryota
ENOG410Y2AP LUCA
GeneTreeiENSGT00900000140901
HOVERGENiHBG058247
InParanoidiA2CG63
KOiK19195
OMAiTHTRNDL
OrthoDBiEOG091G0EG4
PhylomeDBiA2CG63
TreeFamiTF106427

Enzyme and pathway databases

ReactomeiR-MMU-3214815 HDACs deacetylate histones

Miscellaneous databases

ChiTaRSiArid4b mouse
PROiPR:A2CG63
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000039219 Expressed in 270 organ(s), highest expression level in supraoptic nucleus
CleanExiMM_ARID4B
ExpressionAtlasiA2CG63 baseline and differential
GenevisibleiA2CG63 MM

Family and domain databases

CDDicd00024 CHROMO, 1 hit
Gene3Di1.10.150.60, 1 hit
InterProiView protein in InterPro
IPR028853 ARID4B
IPR001606 ARID_dom
IPR036431 ARID_dom_sf
IPR016197 Chromo-like_dom_sf
IPR000953 Chromo/chromo_shadow_dom
IPR012603 RBB1NT
IPR002999 Tudor
IPR025995 Tudor-knot
PANTHERiPTHR13964:SF24 PTHR13964:SF24, 1 hit
PfamiView protein in Pfam
PF01388 ARID, 1 hit
PF08169 RBB1NT, 1 hit
PF11717 Tudor-knot, 1 hit
SMARTiView protein in SMART
SM00501 BRIGHT, 1 hit
SM00333 TUDOR, 2 hits
SUPFAMiSSF46774 SSF46774, 1 hit
SSF54160 SSF54160, 1 hit
PROSITEiView protein in PROSITE
PS51011 ARID, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiARI4B_MOUSE
AccessioniPrimary (citable) accession number: A2CG63
Secondary accession number(s): A2CG61
, A2CG62, A2CG64, B2RQ67, Q8BMI8, Q8BYA5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: February 20, 2007
Last modified: November 7, 2018
This is version 102 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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