UniProtKB - A2CG63 (ARI4B_MOUSE)
Protein
AT-rich interactive domain-containing protein 4B
Gene
Arid4b
Organism
Mus musculus (Mouse)
Status
Functioni
Acts as a transcriptional repressor. May function in the assembly and/or enzymatic activity of the Sin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes (By similarity). Plays a role in the regulation of epigenetic modifications at the PWS/AS imprinting center near the SNRPN promoter, where it might function as part of a complex with RB1 and ARID4A (PubMed:17043311). Involved in spermatogenesis, together with ARID4A, where it functions as a transcriptional coactivator for AR (androgen receptor) and enhances expression of genes required for sperm maturation. Regulates expression of the tight junction protein CLDN3 in the testis, which is important for integrity of the blood-testis barrier (PubMed:23487765). Plays a role in myeloid homeostasis where it regulates the histone methylation state of bone marrow cells and expression of various genes involved in hematopoiesis. May function as a leukemia suppressor (PubMed:18728284).By similarity3 Publications
GO - Molecular functioni
- transcription regulatory region sequence-specific DNA binding Source: GO_Central
GO - Biological processi
- establishment of Sertoli cell barrier Source: MGI
- histone H3-K9 trimethylation Source: MGI
- histone H4-K20 trimethylation Source: MGI
- positive regulation of transcription by RNA polymerase II Source: MGI
- regulation of gene expression by genetic imprinting Source: MGI
- regulation of transcription by RNA polymerase II Source: GO_Central
- spermatogenesis Source: MGI
Keywordsi
Molecular function | Chromatin regulator, DNA-binding |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-MMU-3214815, HDACs deacetylate histones |
Names & Taxonomyi
Protein namesi | Recommended name: AT-rich interactive domain-containing protein 4BShort name: ARID domain-containing protein 4B Alternative name(s): 180 kDa Sin3-associated polypeptide Short name: Sin3-associated polypeptide p180 Histone deacetylase complex subunit SAP180 |
Gene namesi | Name:Arid4b Synonyms:Sap180 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2137512, Arid4b |
Subcellular locationi
Keywords - Cellular componenti
NucleusPathology & Biotechi
Disruption phenotypei
Embryonic lethal. Lethality occurs between embryonic day E3.5 and E7.5 (PubMed:17043311). Heterozygous males are fully fertile (PubMed:23487765). Double knockouts of ARID4B heterozygotes with ARID4A homozygotes show various hematological abnormalities, which are more severe than the ARID4A phenotype alone. Hematopoietic stem cell (HSC) and common myeloid progenitor (CMP) counts in bone marrow and spleen are increased. Lymphocyte numbers in spleen are significantly reduced. In peripheral blood, red blood cell counts and lymphocyte counts are reduced. Approximately 83% of animals develop acute myeloid leukemia (AML) and/or myeloid sarcoma. In bone marrow cells, expression of FOXP3 is significantly reduced (PubMed:18728284). Expression of HOXB3, HOXB5, HOXB6, HOXB8 and PITX2 in bone marrow cells is reduced (PubMed:17043311). Males show progressive reduction in fertility from 2 months of age onwards, with reduced testis size and variable defects in seminal vesicle formation. Spermatogenesis is partially blocked from the meiosis II stage onwards leading to reduced numbers of mature spermatozoa. Expression in testis of CLDN3, an androgen receptor-regulated gene, is significantly reduced. Expression of PTGDS is also reduced, whereas expression of INHA and EMB is moderately increased (PubMed:23487765).3 Publications
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000282864 | 1 – 1314 | AT-rich interactive domain-containing protein 4BAdd BLAST | 1314 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 276 | PhosphoserineBy similarity | 1 | |
Modified residuei | 295 | PhosphoserineBy similarity | 1 | |
Modified residuei | 296 | PhosphoserineBy similarity | 1 | |
Cross-linki | 428 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 461 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 482 | PhosphoserineBy similarity | 1 | |
Modified residuei | 666 | PhosphoserineBy similarity | 1 | |
Modified residuei | 668 | PhosphoserineCombined sources | 1 | |
Modified residuei | 675 | PhosphoserineCombined sources | 1 | |
Modified residuei | 717 | PhosphoserineBy similarity | 1 | |
Cross-linki | 751 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 778 | PhosphoserineBy similarity | 1 | |
Modified residuei | 790 | PhosphoserineCombined sources | 1 | |
Modified residuei | 793 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 1016 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1028 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 1031 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1152 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 1154 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1155 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1157 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1161 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | A2CG63 |
jPOSTi | A2CG63 |
MaxQBi | A2CG63 |
PaxDbi | A2CG63 |
PeptideAtlasi | A2CG63 |
PRIDEi | A2CG63 |
PTM databases
iPTMneti | A2CG63 |
PhosphoSitePlusi | A2CG63 |
Expressioni
Tissue specificityi
Expressed in Sertoli cells of the testis.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000039219, Expressed in forelimb bud and 285 other tissues |
Genevisiblei | A2CG63, MM |
Interactioni
Subunit structurei
Binary interactionsi
A2CG63
With | #Exp. | IntAct |
---|---|---|
Stra8 [P70278] | 4 | EBI-6394082,EBI-6394115 |
Protein-protein interaction databases
BioGRIDi | 220492, 8 interactors |
ComplexPortali | CPX-3441, SIN3A histone deacetylase complex, ES cell-specific variant CPX-3443, SIN3A histone deacetylase complex CPX-3444, SIN3B histone deacetylase complex |
IntActi | A2CG63, 2 interactors |
STRINGi | 10090.ENSMUSP00000106163 |
Miscellaneous databases
RNActi | A2CG63, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 306 – 398 | ARIDPROSITE-ProRule annotationAdd BLAST | 93 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 1227 – 1272 | Sequence analysisAdd BLAST | 46 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 154 – 159 | Poly-Ser | 6 | |
Compositional biasi | 268 – 567 | Glu-richAdd BLAST | 300 | |
Compositional biasi | 1097 – 1103 | Poly-Ser | 7 | |
Compositional biasi | 1273 – 1305 | Ser-richAdd BLAST | 33 |
Domaini
The C-terminus mediates interaction with mSin3A corepressor complex.By similarity
The N-terminus is involved in transcriptional repression by HDAC-independent mechanisms.By similarity
The ARID domain is involved in stabilizing the mSin3A corepressor complex on DNA.By similarity
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG2744, Eukaryota KOG3001, Eukaryota |
GeneTreei | ENSGT00940000158149 |
HOGENOMi | CLU_007419_0_0_1 |
InParanoidi | A2CG63 |
OMAi | XCTGQKR |
OrthoDBi | 329541at2759 |
PhylomeDBi | A2CG63 |
TreeFami | TF106427 |
Family and domain databases
Gene3Di | 1.10.150.60, 1 hit |
InterProi | View protein in InterPro IPR028853, ARID4B IPR001606, ARID_dom IPR036431, ARID_dom_sf IPR016197, Chromo-like_dom_sf IPR012603, RBB1NT IPR002999, Tudor IPR025995, Tudor-knot |
PANTHERi | PTHR13964:SF24, PTHR13964:SF24, 1 hit |
Pfami | View protein in Pfam PF01388, ARID, 1 hit PF08169, RBB1NT, 1 hit PF11717, Tudor-knot, 1 hit |
SMARTi | View protein in SMART SM00501, BRIGHT, 1 hit SM00333, TUDOR, 2 hits |
SUPFAMi | SSF46774, SSF46774, 1 hit SSF54160, SSF54160, 1 hit |
PROSITEi | View protein in PROSITE PS51011, ARID, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: A2CG63-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MKALDEPPYL TVGTDVSAKY RGAFCEAKIK TAKRLVKVKV TFRHDSSTVE
60 70 80 90 100
VQDDHIKGPL KVGAIVEVKN LDGAYQEAVI NKLTDASWYT VVFDDGDEKT
110 120 130 140 150
LRRSSLCLKG ERHFAESETL DQLPLTNPEH FGTPVIGKKT NRGRRSNHIP
160 170 180 190 200
EEESSSSSSD DDEEERKQTD ELLGKVVCVD YVSLEKKKAM WFPALVVCPD
210 220 230 240 250
CSDEIAVKKD NILVRSFKDG KFTSVPRKDV HEITSDTVPK PDAVLKQAFD
260 270 280 290 300
QALEFHKSRA IPANWKTELK EDSSSSEAEE EEEEEDDEKE KEDNSSEEEE
310 320 330 340 350
EIEPFPEERE NFLQQLYKFM EDRGTPINKR PVLGYRNLNL FKLFRLVHKL
360 370 380 390 400
GGFDNIESGA VWKQVYQDLG IPVLNSAAGY NVKCAYKKYL YGFEEYCRSA
410 420 430 440 450
NIDFQMALPE KVLNKPCKDC ENKEVKVKEE SETEIKEVNV EDSKNVMPKE
460 470 480 490 500
ETPAEDESER KENIKPSLGS KKSLLECIPA QSDEEKEAHI TKLEENENLE
510 520 530 540 550
DKDGGRARTE EAFSTEVDGE EEQARSGDET NKEEDEDDEE IEEEEEEDEE
560 570 580 590 600
EDEDEDDDDN NEEEEFECYP PGMKVQVRYG RGKNQKMYEA SIKDSDVEGG
610 620 630 640 650
EALYLVHYCG WNVRYDEWIK ADKIVRPADK NVPKIKHRKK IKNKLDKEKD
660 670 680 690 700
RDEKYSPKNC KLRRLSKSPF QSNPSPEMVS KLDLADAKNS DTAHIKSIEI
710 720 730 740 750
TSILNGLQAS ESSAEDSEQE DERCTQDVDN IGKDESKVEH STHSRNELIS
760 770 780 790 800
KEEQSSPSLL EENKVHTDLV IAKTVSKSPE RLRKDMEAIS EDTDFEEEDE
810 820 830 840 850
ITKKRKDVKK DTTDKALKPQ TKRGKRRYCS ADECLQTGSP GKKEDRTKSK
860 870 880 890 900
EPLCTENSSN SSSDEDEEEK SKAKMTPTKK YNGLEEKRKS LRTTSFYSGF
910 920 930 940 950
SEVAEKRIKL LNNSDERLQN NRAKDRKDVW SSIQGQWPKK TLKELFSDSD
960 970 980 990 1000
TEAAASPPHP APDEGAVEES LQTVAEEESC SPIMELEKPL PASVDNKPIE
1010 1020 1030 1040 1050
EKPLEVSDRK TEFPSSGSNS VLNTPPTTPE SPSSVTITEA SQQQSSVTVS
1060 1070 1080 1090 1100
VPLPPNQEEV RSIKSETDST IEVDSVVGEL QDLQSEGNSS PAGFDASVSS
1110 1120 1130 1140 1150
SSSNQPEPDN PEKACTGQKR VKDTQGVGSS SKKQKRSHKA TVVNNKKKGK
1160 1170 1180 1190 1200
GTNSSDSEEL SAGESVTKTQ TIKSVPTGMK THNSKSPARV QSPGKGGRNG
1210 1220 1230 1240 1250
DKDPDLKEPS NRLPKVYKWS FQTSDLENMT SAERISILQE KLQEIRKHYL
1260 1270 1280 1290 1300
SLKSEVASID RRRKRLKKKE RESAATSSSS SSPSSSSITA AVMLTLAEPS
1310
MSSASQNGMS VECR
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketZ4YL44 | Z4YL44_MOUSE | AT-rich interactive domain-containi... | Arid4b | 473 | Annotation score: | ||
Z4YMH1 | Z4YMH1_MOUSE | AT-rich interactive domain-containi... | Arid4b | 540 | Annotation score: | ||
A0A1Y7VIX3 | A0A1Y7VIX3_MOUSE | AT-rich interactive domain-containi... | Arid4b | 128 | Annotation score: | ||
A0A1Y7VKA9 | A0A1Y7VKA9_MOUSE | AT-rich interactive domain-containi... | Arid4b | 31 | Annotation score: |
Sequence cautioni
The sequence CAM21242 differs from that shown. Reason: Erroneous gene model prediction.Curated
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_024236 | 528 – 614 | Missing in isoform 2. CuratedAdd BLAST | 87 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CT025604 Genomic DNA Translation: CAM21242.1 Sequence problems. CT025604 Genomic DNA Translation: CAM21243.1 CT025604 Genomic DNA Translation: CAM21244.1 CT025604 Genomic DNA Translation: CAM21245.1 BC137783 mRNA Translation: AAI37784.1 AK031062 mRNA Translation: BAC27233.1 AK041414 mRNA Translation: BAC30936.1 |
CCDSi | CCDS36599.1 [A2CG63-1] CCDS36600.1 [A2CG63-2] |
RefSeqi | NP_919238.1, NM_194262.2 [A2CG63-1] NP_937755.1, NM_198122.2 [A2CG63-2] XP_006516869.1, XM_006516806.2 [A2CG63-1] XP_006516870.1, XM_006516807.2 [A2CG63-1] XP_017171137.1, XM_017315648.1 [A2CG63-1] |
Genome annotation databases
Ensembli | ENSMUST00000039538; ENSMUSP00000043889; ENSMUSG00000039219 [A2CG63-2] ENSMUST00000110534; ENSMUSP00000106163; ENSMUSG00000039219 [A2CG63-1] ENSMUST00000110536; ENSMUSP00000106165; ENSMUSG00000039219 [A2CG63-2] |
GeneIDi | 94246 |
KEGGi | mmu:94246 |
UCSCi | uc007pmz.2, mouse [A2CG63-1] uc007pna.2, mouse [A2CG63-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CT025604 Genomic DNA Translation: CAM21242.1 Sequence problems. CT025604 Genomic DNA Translation: CAM21243.1 CT025604 Genomic DNA Translation: CAM21244.1 CT025604 Genomic DNA Translation: CAM21245.1 BC137783 mRNA Translation: AAI37784.1 AK031062 mRNA Translation: BAC27233.1 AK041414 mRNA Translation: BAC30936.1 |
CCDSi | CCDS36599.1 [A2CG63-1] CCDS36600.1 [A2CG63-2] |
RefSeqi | NP_919238.1, NM_194262.2 [A2CG63-1] NP_937755.1, NM_198122.2 [A2CG63-2] XP_006516869.1, XM_006516806.2 [A2CG63-1] XP_006516870.1, XM_006516807.2 [A2CG63-1] XP_017171137.1, XM_017315648.1 [A2CG63-1] |
3D structure databases
SMRi | A2CG63 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 220492, 8 interactors |
ComplexPortali | CPX-3441, SIN3A histone deacetylase complex, ES cell-specific variant CPX-3443, SIN3A histone deacetylase complex CPX-3444, SIN3B histone deacetylase complex |
IntActi | A2CG63, 2 interactors |
STRINGi | 10090.ENSMUSP00000106163 |
PTM databases
iPTMneti | A2CG63 |
PhosphoSitePlusi | A2CG63 |
Proteomic databases
EPDi | A2CG63 |
jPOSTi | A2CG63 |
MaxQBi | A2CG63 |
PaxDbi | A2CG63 |
PeptideAtlasi | A2CG63 |
PRIDEi | A2CG63 |
Protocols and materials databases
Antibodypediai | 20807, 148 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000039538; ENSMUSP00000043889; ENSMUSG00000039219 [A2CG63-2] ENSMUST00000110534; ENSMUSP00000106163; ENSMUSG00000039219 [A2CG63-1] ENSMUST00000110536; ENSMUSP00000106165; ENSMUSG00000039219 [A2CG63-2] |
GeneIDi | 94246 |
KEGGi | mmu:94246 |
UCSCi | uc007pmz.2, mouse [A2CG63-1] uc007pna.2, mouse [A2CG63-2] |
Organism-specific databases
CTDi | 51742 |
MGIi | MGI:2137512, Arid4b |
Phylogenomic databases
eggNOGi | KOG2744, Eukaryota KOG3001, Eukaryota |
GeneTreei | ENSGT00940000158149 |
HOGENOMi | CLU_007419_0_0_1 |
InParanoidi | A2CG63 |
OMAi | XCTGQKR |
OrthoDBi | 329541at2759 |
PhylomeDBi | A2CG63 |
TreeFami | TF106427 |
Enzyme and pathway databases
Reactomei | R-MMU-3214815, HDACs deacetylate histones |
Miscellaneous databases
BioGRID-ORCSi | 94246, 5 hits in 20 CRISPR screens |
ChiTaRSi | Arid4b, mouse |
PROi | PR:A2CG63 |
RNActi | A2CG63, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000039219, Expressed in forelimb bud and 285 other tissues |
Genevisiblei | A2CG63, MM |
Family and domain databases
Gene3Di | 1.10.150.60, 1 hit |
InterProi | View protein in InterPro IPR028853, ARID4B IPR001606, ARID_dom IPR036431, ARID_dom_sf IPR016197, Chromo-like_dom_sf IPR012603, RBB1NT IPR002999, Tudor IPR025995, Tudor-knot |
PANTHERi | PTHR13964:SF24, PTHR13964:SF24, 1 hit |
Pfami | View protein in Pfam PF01388, ARID, 1 hit PF08169, RBB1NT, 1 hit PF11717, Tudor-knot, 1 hit |
SMARTi | View protein in SMART SM00501, BRIGHT, 1 hit SM00333, TUDOR, 2 hits |
SUPFAMi | SSF46774, SSF46774, 1 hit SSF54160, SSF54160, 1 hit |
PROSITEi | View protein in PROSITE PS51011, ARID, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ARI4B_MOUSE | |
Accessioni | A2CG63Primary (citable) accession number: A2CG63 Secondary accession number(s): A2CG61 Q8BYA5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 3, 2007 |
Last sequence update: | February 20, 2007 | |
Last modified: | December 2, 2020 | |
This is version 115 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot