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Protein

Thiamine thiazole synthase

Gene

thi4

Organism
Hyperthermus butylicus (strain DSM 5456 / JCM 9403 / PLM1-5)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the thiazole moiety of thiamine. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylate (ADT), an adenylated thiazole intermediate, using free sulfide as a source of sulfur.UniRule annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Cofactori

Fe2+UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei53NAD; via amide nitrogenUniRule annotation1
Binding sitei80NAD; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei145NAD; via amide nitrogen and carbonyl oxygenUniRule annotation1
Metal bindingi177Iron; shared with adjacent protomerUniRule annotation1
Metal bindingi192IronUniRule annotation1
Binding sitei242NAD; via amide nitrogenUniRule annotation1
Binding sitei252GlycineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi175 – 177NAD; shared with adjacent protomerUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyaseUniRule annotation
Biological processThiamine biosynthesisUniRule annotation
LigandIronUniRule annotation, Metal-bindingUniRule annotation, NADUniRule annotation

Enzyme and pathway databases

BioCyciHBUT415426:GC56-253-MONOMER
UniPathwayiUPA00060

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine thiazole synthaseUniRule annotation (EC:4.-.-.-UniRule annotation)
Gene namesi
Name:thi4UniRule annotation
Ordered Locus Names:Hbut_0253Imported
OrganismiHyperthermus butylicus (strain DSM 5456 / JCM 9403 / PLM1-5)Imported
Taxonomic identifieri415426 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiDesulfurococcalesPyrodictiaceaeHyperthermus
Proteomesi
  • UP000002593 Componenti: Chromosome

Interactioni

Subunit structurei

Homooctamer; tetramer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi415426.Hbut_0253

Structurei

3D structure databases

ProteinModelPortaliA2BJG4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the THI4 family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00574 Archaea
COG1635 LUCA
HOGENOMiHOG000106048
KOiK22699
OMAiMTEFTDI
OrthoDBiPOG093Z09PU

Family and domain databases

Gene3Di3.50.50.60, 1 hit
HAMAPiMF_00304 Thi4, 1 hit
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR002922 Thi4_fam
IPR022828 Thi4_prok
SUPFAMiSSF51905 SSF51905, 1 hit
TIGRFAMsiTIGR00292 TIGR00292, 1 hit

Sequencei

Sequence statusi: Complete.

A2BJG4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVNAVQAPEH SWLPHNVTSL REGALAALII RKTAEKLTSI TSVDVAIAGA
60 70 80 90 100
GPAGLTAAWL LAEKGLRVVV VEHSLGVGGG MRGGSMLMPV GLVEDGLPAE
110 120 130 140 150
LLRRAGARLD RVADGLYAVD PTEAVVKLAA KAIDAGAVIL PGLHVEDLIL
160 170 180 190 200
WRSGSGYRVA GLVINLSPVV EAGWHVDPIY IEARATIDAT GHDAELVKLL
210 220 230 240 250
SKALGDSSIR VRGTRGMDVW EGEKLVVEYT GEVYPGLYAA GMAVSETYQL
260 270
PRMGPVFGGM LASGARVAEL VASRLSEQ
Length:278
Mass (Da):29,084
Last modified:February 20, 2007 - v1
Checksum:iDA1D569BACF02AC0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000493 Genomic DNA Translation: ABM80125.1

Genome annotation databases

EnsemblBacteriaiABM80125; ABM80125; Hbut_0253
KEGGihbu:Hbut_0253

Similar proteinsi

Entry informationi

Entry nameiA2BJG4_HYPBU
AccessioniPrimary (citable) accession number: A2BJG4
Entry historyiIntegrated into UniProtKB/TrEMBL: February 20, 2007
Last sequence update: February 20, 2007
Last modified: July 18, 2018
This is version 66 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

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