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Protein

S-methyl-5'-thioadenosine phosphorylase

Gene

mtnP

Organism
Hyperthermus butylicus (strain DSM 5456 / JCM 9403 / PLM1-5)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.UniRule annotation

Catalytic activityi

S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation

Pathwayi: L-methionine biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. S-methyl-5'-thioadenosine phosphorylase (mtnP)
This subpathway is part of the pathway L-methionine biosynthesis via salvage pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route), the pathway L-methionine biosynthesis via salvage pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei20PhosphateUniRule annotation1
Sitei175Important for substrate specificityUniRule annotation1
Binding sitei194Substrate; via amide nitrogenUniRule annotation1
Binding sitei195PhosphateUniRule annotation1
Sitei229Important for substrate specificityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processPurine salvage

Enzyme and pathway databases

UniPathwayi
UPA00904;UER00873

Names & Taxonomyi

Protein namesi
Recommended name:
S-methyl-5'-thioadenosine phosphorylaseUniRule annotation (EC:2.4.2.28UniRule annotation)
Alternative name(s):
5'-methylthioadenosine phosphorylaseUniRule annotation
Short name:
MTA phosphorylaseUniRule annotation
Short name:
MTAPUniRule annotation
Gene namesi
Name:mtnPUniRule annotation
Ordered Locus Names:Hbut_0022
OrganismiHyperthermus butylicus (strain DSM 5456 / JCM 9403 / PLM1-5)
Taxonomic identifieri415426 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiDesulfurococcalesPyrodictiaceaeHyperthermus
Proteomesi
  • UP000002593 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004151041 – 274S-methyl-5'-thioadenosine phosphorylaseAdd BLAST274

Interactioni

Subunit structurei

Homohexamer. Dimer of a homotrimer.UniRule annotation

Protein-protein interaction databases

STRINGi415426.Hbut_0022

Structurei

3D structure databases

ProteinModelPortaliA2BIU4
SMRiA2BIU4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni62 – 63Phosphate bindingUniRule annotation2
Regioni95 – 96Phosphate bindingUniRule annotation2
Regioni218 – 220Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01327 Archaea
COG0005 LUCA
HOGENOMiHOG000228987
KOiK00772
OMAiNLYRSWG
OrthoDBiPOG093Z08FG

Family and domain databases

HAMAPiMF_01963 MTAP, 1 hit
InterProiView protein in InterPro
IPR010044 MTAP
IPR000845 Nucleoside_phosphorylase_d
IPR035994 Nucleoside_phosphorylase_sf
IPR018099 Purine_phosphorylase-2_CS
PANTHERiPTHR42679 PTHR42679, 1 hit
PfamiView protein in Pfam
PF01048 PNP_UDP_1, 1 hit
SUPFAMiSSF53167 SSF53167, 1 hit
TIGRFAMsiTIGR01694 MTAP, 1 hit
PROSITEiView protein in PROSITE
PS01240 PNP_MTAP_2, 1 hit

Sequencei

Sequence statusi: Complete.

A2BIU4-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPDFPPKPSV KADIAIIGGS GLYDPGVLEN AKEYKIYTPY GEPSDYIIVG
60 70 80 90 100
ELGGKRVAFL PRHGRGHRIP PHKINYRANI WALKMLGVKW VIAVSAVGSL
110 120 130 140 150
REDYKPGDIV VPDQFIDMTK SRVYTFFDEG IVAHVSMADP FCEHLRQEII
160 170 180 190 200
STARELGYRV HPRGTYICIE GPRFSTRAES RVWREVFKAD IIGMTLVPEV
210 220 230 240 250
NLACEAQLCY ATIALVTDYD VWAERPVTAE EVARVMKENT EKAKKILYKL
260 270
IPRLPPEPDK ARCSCCSSLE TALL
Length:274
Mass (Da):30,784
Last modified:February 20, 2007 - v1
Checksum:i305ED09C45DDCE01
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000493 Genomic DNA Translation: ABM79900.1
RefSeqiWP_011821217.1, NC_008818.1

Genome annotation databases

EnsemblBacteriaiABM79900; ABM79900; Hbut_0022
GeneIDi4782054
KEGGihbu:Hbut_0022

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000493 Genomic DNA Translation: ABM79900.1
RefSeqiWP_011821217.1, NC_008818.1

3D structure databases

ProteinModelPortaliA2BIU4
SMRiA2BIU4
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi415426.Hbut_0022

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABM79900; ABM79900; Hbut_0022
GeneIDi4782054
KEGGihbu:Hbut_0022

Phylogenomic databases

eggNOGiarCOG01327 Archaea
COG0005 LUCA
HOGENOMiHOG000228987
KOiK00772
OMAiNLYRSWG
OrthoDBiPOG093Z08FG

Enzyme and pathway databases

UniPathwayi
UPA00904;UER00873

Family and domain databases

HAMAPiMF_01963 MTAP, 1 hit
InterProiView protein in InterPro
IPR010044 MTAP
IPR000845 Nucleoside_phosphorylase_d
IPR035994 Nucleoside_phosphorylase_sf
IPR018099 Purine_phosphorylase-2_CS
PANTHERiPTHR42679 PTHR42679, 1 hit
PfamiView protein in Pfam
PF01048 PNP_UDP_1, 1 hit
SUPFAMiSSF53167 SSF53167, 1 hit
TIGRFAMsiTIGR01694 MTAP, 1 hit
PROSITEiView protein in PROSITE
PS01240 PNP_MTAP_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMTAP_HYPBU
AccessioniPrimary (citable) accession number: A2BIU4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: February 20, 2007
Last modified: February 28, 2018
This is version 61 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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