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UniProtKB - A2AQ99 (A2AQ99_MOUSE)
Protein
NAD(P)H oxidase (H(2)O(2)-forming)
Gene
Duox2
Organism
Mus musculus (Mouse)
Status
Functioni
Generates hydrogen peroxide which is required for the activity of thyroid peroxidase/TPO and lactoperoxidase/LPO. Plays a role in thyroid hormones synthesis and lactoperoxidase-mediated antimicrobial defense at the surface of mucosa. May have its own peroxidase activity through its N-terminal peroxidase-like domain.
ARBA annotationCatalytic activityi
: thyroid hormone biosynthesis Pathwayi
This protein is involved in the pathway thyroid hormone biosynthesis, which is part of Hormone biosynthesis.ARBA annotationView all proteins of this organism that are known to be involved in the pathway thyroid hormone biosynthesis and in Hormone biosynthesis.
GO - Molecular functioni
- calcium ion binding Source: MGI
- heme binding Source: InterPro
- NAD(P)H oxidase H2O2-forming activity Source: MGI
- NADH oxidase H202-forming activity Source: RHEA
- NADPH oxidase H202-forming activity Source: RHEA
- peroxidase activity Source: UniProtKB-KW
- superoxide-generating NAD(P)H oxidase activity Source: GO_Central
GO - Biological processi
- adenohypophysis morphogenesis Source: MGI
- bone mineralization Source: MGI
- cytokine-mediated signaling pathway Source: MGI
- defense response Source: GO_Central
- fertilization Source: MGI
- hormone biosynthetic process Source: UniProtKB-KW
- hormone metabolic process Source: MGI
- hydrogen peroxide biosynthetic process Source: MGI
- inner ear development Source: MGI
- multicellular organism growth Source: MGI
- positive regulation of cell motility Source: MGI
- positive regulation of wound healing Source: MGI
- response to cAMP Source: MGI
- response to oxidative stress Source: InterPro
- response to virus Source: MGI
- superoxide anion generation Source: MGI
- thyroid gland development Source: MGI
- thyroid hormone generation Source: UniProtKB-UniPathway
- thyroid hormone metabolic process Source: MGI
Keywordsi
Molecular function | Oxidoreductase, PeroxidaseARBA annotation |
Biological process | Thyroid hormones biosynthesisARBA annotation |
Ligand | FADARBA annotation, Flavoprotein, Metal-bindingARBA annotation, NADPARBA annotation |
Enzyme and pathway databases
UniPathwayi | UPA00194 |
Protein family/group databases
PeroxiBasei | 3341, MmDuOx02-4 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Duox2Imported |
Organismi | Mus musculus (Mouse)Imported |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:3036280, Duox2 |
VEuPathDBi | HostDB:ENSMUSG00000068452 |
Subcellular locationi
Plasma membrane
- Apical cell membrane ARBA annotation; Multi-pass membrane protein ARBA annotation
Other locations
- Membrane ARBA annotation; Multi-pass membrane protein ARBA annotation
Cytosol
- cytosol Source: MGI
Endoplasmic reticulum
- endoplasmic reticulum Source: MGI
Plasma Membrane
- apical plasma membrane Source: UniProtKB-SubCell
- NADPH oxidase complex Source: GO_Central
- plasma membrane Source: MGI
Other locations
- apical part of cell Source: MGI
- cell leading edge Source: MGI
- cell surface Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 596 – 622 | HelicalSequence analysisAdd BLAST | 27 | |
Transmembranei | 1007 – 1026 | HelicalSequence analysisAdd BLAST | 20 | |
Transmembranei | 1046 – 1068 | HelicalSequence analysisAdd BLAST | 23 | |
Transmembranei | 1148 – 1175 | HelicalSequence analysisAdd BLAST | 28 | |
Transmembranei | 1187 – 1209 | HelicalSequence analysisAdd BLAST | 23 |
Keywords - Cellular componenti
MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 25 | Sequence analysisAdd BLAST | 25 | |
ChainiPRO_5002642732 | 26 – 1517 | NAD(P)H oxidase (H(2)O(2)-forming)Sequence analysisAdd BLAST | 1492 |
Proteomic databases
PaxDbi | A2AQ99 |
ProteomicsDBi | 349677 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000068452, Expressed in ileum and 43 other tissues |
ExpressionAtlasi | A2AQ99, baseline and differential |
Interactioni
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000050314 |
Miscellaneous databases
RNActi | A2AQ99, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 819 – 854 | EF-handInterPro annotationAdd BLAST | 36 | |
Domaini | 855 – 890 | EF-handInterPro annotationAdd BLAST | 36 | |
Domaini | 1236 – 1342 | FAD-binding FR-typeInterPro annotationAdd BLAST | 107 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 153 – 175 | DisorderedSequence analysisAdd BLAST | 23 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 154 – 175 | Polar residuesSequence analysisAdd BLAST | 22 |
Sequence similaritiesi
In the N-terminal section; belongs to the peroxidase family.ARBA annotation
Keywords - Domaini
RepeatARBA annotation, SignalSequence analysis, Transmembrane, Transmembrane helixSequence analysisARBA annotationPhylogenomic databases
eggNOGi | KOG0039, Eukaryota |
GeneTreei | ENSGT00940000160291 |
HOGENOMi | CLU_004482_1_0_1 |
InParanoidi | A2AQ99 |
OMAi | GHAVNIY |
OrthoDBi | 27424at2759 |
PhylomeDBi | A2AQ99 |
TreeFami | TF105424 |
Family and domain databases
CDDi | cd09820, dual_peroxidase_like, 1 hit cd00051, EFh, 2 hits |
Gene3Di | 1.10.640.10, 1 hit 3.40.50.80, 1 hit |
InterProi | View protein in InterPro IPR034818, DUOX2 IPR034821, DUOX_peroxidase IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom IPR013112, FAD-bd_8 IPR017927, FAD-bd_FR_type IPR013130, Fe3_Rdtase_TM_dom IPR013121, Fe_red_NAD-bd_6 IPR039261, FNR_nucleotide-bd IPR019791, Haem_peroxidase_animal IPR010255, Haem_peroxidase_sf IPR037120, Haem_peroxidase_sf_animal IPR017938, Riboflavin_synthase-like_b-brl |
PANTHERi | PTHR11972:SF67, PTHR11972:SF67, 1 hit |
Pfami | View protein in Pfam PF03098, An_peroxidase, 1 hit PF00036, EF-hand_1, 1 hit PF13202, EF-hand_5, 1 hit PF08022, FAD_binding_8, 1 hit PF01794, Ferric_reduct, 1 hit PF08030, NAD_binding_6, 1 hit |
PRINTSi | PR00457, ANPEROXIDASE |
SMARTi | View protein in SMART SM00054, EFh, 2 hits |
SUPFAMi | SSF47473, SSF47473, 1 hit SSF48113, SSF48113, 1 hit SSF52343, SSF52343, 1 hit SSF63380, SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS00018, EF_HAND_1, 2 hits PS50222, EF_HAND_2, 2 hits PS51384, FAD_FR, 1 hit PS50292, PEROXIDASE_3, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
A2AQ99-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLPTSPKTLV LLGALLTGPL GPAGGQDAPS LPWEVQRYDG WFNNLKYHQR
60 70 80 90 100
GAAGSRLRRL IPANYADGVY QALEEPLLPN PRRLSDAVAK GKAGLPSVHN
110 120 130 140 150
RTVLGVFFGY HVLSDLVSVE TPGCPAEFLN IYIPRGDPVF DPDKRGNVVL
160 170 180 190 200
PFQRSRWDHN TGQSPSNPRD QSNQVTGWLD GSAIYGSSHS WSDTLRSFSG
210 220 230 240 250
GQLASGPDPA FPRNSQSSLL MWMAPDPSTG RGGPQGVYAF GAQRGNREPF
260 270 280 290 300
LQALGLLWFR YHNLCARKLA QEHPHWGDEE LFQHARKRVI ATYQNIALYQ
310 320 330 340 350
WLPSFLQKTP PEYSGYRPFM DPSISPEFVV ASEQFLSTMV PPGVYMRNSS
360 370 380 390 400
CHFRKFPKEG SDSSPALRVC NSYWIRENPN LKTAQDVDQL LLGMASQISE
410 420 430 440 450
LEDRIVIEDL RDYWPGPERF SRTDYVASSI QRGRDMGLPS YSQALLALGL
460 470 480 490 500
EPPKNWSALN PQVEPQVLEA TAALYNQDLS QLELLLGGLL ESHGDPGPLF
510 520 530 540 550
SNIILDQFVR LRDGDRYWFE NTRNGLFSKE EIAEIRNTTL RDVLVAVSNV
560 570 580 590 600
DPSALQPNVF FWQEGAPCPQ PRQLTTDGLP QCAPVTVIDY FEGSGAGYGV
610 620 630 640 650
TLVAVCCFPL VSLIVAGVVA HFRNREHKML LKKGKESLKK QPASDGVPAM
660 670 680 690 700
EWPGPKEKSY PVTLQLLPDR SLQVLDKRFT VLRTIQLQSP QQVNLILSSN
710 720 730 740 750
SGRRTLLLKI PKEYDLVLMF NSEEDRGAFV RLLQDLCICC TPGLHIAEVD
760 770 780 790 800
EKELLRKAVT KQQRAGILEI FFRQLFAQVL DINQADAGTL PLDSSQQVRE
810 820 830 840 850
ALTCELSRAE FADSLGLKPQ DMFVESMFSL ADKDGNGYIS FREFLDILVV
860 870 880 890 900
FMKGSSEDKS RLMFTMYDLD GNGFLSKDEF FTMMRSFIEI SNNCLSKAQL
910 920 930 940 950
AEVVESMFRE SGFQDKEELT WEDFHFMLRD HDSDLRFTQL CVKGGAGGTK
960 970 980 990 1000
DIFKQSSACR VSFINRTPGN RVMGPSPRLY TEALQEKKQS GFLAQKFKQY
1010 1020 1030 1040 1050
KRFVENYRRH IVCVTIFSAI CIGLFADRAY YYGFASPPTD IEETTYVGII
1060 1070 1080 1090 1100
LSRGTAASIS FMFSYILLTM CRNLITFLRE TFLNRYIPFD AAVDFHRWIA
1110 1120 1130 1140 1150
MAAVVLAVLH SAGHAVNVYI FSVSPLSLMA CVFPNVFVND GSKFPPKYYW
1160 1170 1180 1190 1200
WFFETVPGMT GVLLLLVLAI MYVFASHHFR RHSFRGFWLT HHLYVVLYVL
1210 1220 1230 1240 1250
IIIHGSYALI QLPSFHIYFL VPAIIYGGDK LVSLSRKKVE ISVVKAELLP
1260 1270 1280 1290 1300
SGVTYLQFQR PKTFEYKSGQ WVRIACLDLG TNEYHPFTLT SAPHEDTLSL
1310 1320 1330 1340 1350
HIRAVGPWTT RLREIYSPPV GGTCARYPKL YLDGPFGEGH QEWHKFEVSV
1360 1370 1380 1390 1400
LVGGGIGVTP FASILKDLVF KSSMGSQMLC KKIYFIWVTR TQRQFEWLAD
1410 1420 1430 1440 1450
IIREVEENDC QDLVSVHIYI TQLAEKFDLR TTMLYICERH FQKALNRSLF
1460 1470 1480 1490 1500
TGLRSITHFG RPPFELFFNS LQEVHPQVRK IGVFSCGPPG MTKNVEKACQ
1510
LINRQDRAHF VHHYENF
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A494BAW1 | A0A494BAW1_MOUSE | NAD(P)H oxidase (H(2)O(2)-forming) | Duox2 | 1,545 | Annotation score: |
Sequence databases
RefSeqi | NP_808278.2, NM_177610.2 |
Genome annotation databases
Ensembli | ENSMUST00000053734.6; ENSMUSP00000050314.6; ENSMUSG00000068452.9 |
UCSCi | uc008mak.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
RefSeqi | NP_808278.2, NM_177610.2 |
3D structure databases
SMRi | A2AQ99 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000050314 |
Protein family/group databases
PeroxiBasei | 3341, MmDuOx02-4 |
Proteomic databases
PaxDbi | A2AQ99 |
ProteomicsDBi | 349677 |
Protocols and materials databases
Antibodypediai | 24313, 175 antibodies from 21 providers |
Genome annotation databases
Ensembli | ENSMUST00000053734.6; ENSMUSP00000050314.6; ENSMUSG00000068452.9 |
UCSCi | uc008mak.1, mouse |
Organism-specific databases
MGIi | MGI:3036280, Duox2 |
VEuPathDBi | HostDB:ENSMUSG00000068452 |
Phylogenomic databases
eggNOGi | KOG0039, Eukaryota |
GeneTreei | ENSGT00940000160291 |
HOGENOMi | CLU_004482_1_0_1 |
InParanoidi | A2AQ99 |
OMAi | GHAVNIY |
OrthoDBi | 27424at2759 |
PhylomeDBi | A2AQ99 |
TreeFami | TF105424 |
Enzyme and pathway databases
UniPathwayi | UPA00194 |
Miscellaneous databases
BioGRID-ORCSi | 214593, 4 hits in 73 CRISPR screens |
RNActi | A2AQ99, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000068452, Expressed in ileum and 43 other tissues |
ExpressionAtlasi | A2AQ99, baseline and differential |
Family and domain databases
CDDi | cd09820, dual_peroxidase_like, 1 hit cd00051, EFh, 2 hits |
Gene3Di | 1.10.640.10, 1 hit 3.40.50.80, 1 hit |
InterProi | View protein in InterPro IPR034818, DUOX2 IPR034821, DUOX_peroxidase IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom IPR013112, FAD-bd_8 IPR017927, FAD-bd_FR_type IPR013130, Fe3_Rdtase_TM_dom IPR013121, Fe_red_NAD-bd_6 IPR039261, FNR_nucleotide-bd IPR019791, Haem_peroxidase_animal IPR010255, Haem_peroxidase_sf IPR037120, Haem_peroxidase_sf_animal IPR017938, Riboflavin_synthase-like_b-brl |
PANTHERi | PTHR11972:SF67, PTHR11972:SF67, 1 hit |
Pfami | View protein in Pfam PF03098, An_peroxidase, 1 hit PF00036, EF-hand_1, 1 hit PF13202, EF-hand_5, 1 hit PF08022, FAD_binding_8, 1 hit PF01794, Ferric_reduct, 1 hit PF08030, NAD_binding_6, 1 hit |
PRINTSi | PR00457, ANPEROXIDASE |
SMARTi | View protein in SMART SM00054, EFh, 2 hits |
SUPFAMi | SSF47473, SSF47473, 1 hit SSF48113, SSF48113, 1 hit SSF52343, SSF52343, 1 hit SSF63380, SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS00018, EF_HAND_1, 2 hits PS50222, EF_HAND_2, 2 hits PS51384, FAD_FR, 1 hit PS50292, PEROXIDASE_3, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | A2AQ99_MOUSE | |
Accessioni | A2AQ99Primary (citable) accession number: A2AQ99 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | February 20, 2007 |
Last sequence update: | February 20, 2007 | |
Last modified: | May 25, 2022 | |
This is version 130 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) |