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Protein

Transcriptional repressor CTCFL

Gene

Ctcfl

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Testis-specific DNA binding protein responsible for insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. Plays a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region (ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2. This probably leads to recruit de novo DNA methyltransferases at these sites. Seems to act as tumor suppressor. In association with DNMT1 and DNMT3B, involved in activation of BAG1 gene expression by binding to its promoter. Required for dimethylation of H3 lysine 4 (H3K4me2) of MYC and BRCA1 promoters.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri257 – 279C2H2-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri285 – 307C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri313 – 336C2H2-type 3PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri342 – 364C2H2-type 4PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri370 – 392C2H2-type 5PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri398 – 421C2H2-type 6PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri428 – 451C2H2-type 7PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri458 – 480C2H2-type 8PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri486 – 508C2H2-type 9PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri514 – 537C2H2-type 10PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri546 – 572C2H2-type 11PROSITE-ProRule annotationAdd BLAST27

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChromatin regulator, DNA-binding, Repressor
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional repressor CTCFL
Alternative name(s):
Brother of the regulator of imprinted sites
CCCTC-binding factor
CTCF paralog
CTCF-like protein
Gene namesi
Name:Ctcfl
Synonyms:Boris
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:3652571 Ctcfl

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003739241 – 636Transcriptional repressor CTCFLAdd BLAST636

Proteomic databases

MaxQBiA2APF3
PaxDbiA2APF3
PeptideAtlasiA2APF3
PRIDEiA2APF3

PTM databases

iPTMnetiA2APF3
PhosphoSitePlusiA2APF3

Expressioni

Tissue specificityi

Testis-specific.1 Publication

Developmental stagei

Not detected at 13.5 dpc. Detected in mitotically arrested gonocytes of 14.5 dpc embryos. From 17.5 dpc to newborn, it is expressed in some centrally located gonocytes and cells present at the periphery of the developing seminiferous tubules. Present in nuclei of spermatogonia from 15 days after birth to adulthood.1 Publication

Gene expression databases

BgeeiENSMUSG00000070495 Expressed in 25 organ(s), highest expression level in telencephalon

Interactioni

Subunit structurei

Interacts with histones, PRMT7 and SETD1A. Interacts (via N-terminus) with BAG6/BAT3 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Prmt7Q922X93EBI-11566304,EBI-15606508

GO - Molecular functioni

Protein-protein interaction databases

BioGridi576678, 2 interactors
DIPiDIP-29209N
IntActiA2APF3, 5 interactors
STRINGi10090.ENSMUSP00000091845

Structurei

3D structure databases

ProteinModelPortaliA2APF3
SMRiA2APF3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CTCF zinc-finger protein family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri257 – 279C2H2-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri285 – 307C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri313 – 336C2H2-type 3PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri342 – 364C2H2-type 4PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri370 – 392C2H2-type 5PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri398 – 421C2H2-type 6PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri428 – 451C2H2-type 7PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri458 – 480C2H2-type 8PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri486 – 508C2H2-type 9PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri514 – 537C2H2-type 10PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri546 – 572C2H2-type 11PROSITE-ProRule annotationAdd BLAST27

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721 Eukaryota
COG5048 LUCA
GeneTreeiENSGT00890000139371
HOGENOMiHOG000276534
InParanoidiA2APF3
OMAiLRNLHTY
OrthoDBiEOG091G06B4
TreeFamiTF106430

Family and domain databases

InterProiView protein in InterPro
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PfamiView protein in Pfam
PF00096 zf-C2H2, 4 hits
SMARTiView protein in SMART
SM00355 ZnF_C2H2, 11 hits
SUPFAMiSSF57667 SSF57667, 6 hits
PROSITEiView protein in PROSITE
PS00028 ZINC_FINGER_C2H2_1, 7 hits
PS50157 ZINC_FINGER_C2H2_2, 11 hits

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: A2APF3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAAAEVPVPS GYFTQIKEQK LKPGDLEEEK EEDGVQRVEA QEGVVKEVEA
60 70 80 90 100
ENSCLLLEAR APVESDRRIL TLQTVHLESQ DVHLQGLGWL SVPHSEELSG
110 120 130 140 150
TVPEAEGILQ LPSVLWLDPE PQLSLQHCVT VSIPEELYPP EELQRIHFHL
160 170 180 190 200
LRENVLMAEE NPELTPDLDE STALKKPEED EKDQLPPQGE TDKREERLLL
210 220 230 240 250
LEMKPKEGKD DEIVLTISHL SLEEQQDPPA ANQTSVPGAK AAKPKRRRQT
260 270 280 290 300
KGKPQSFQCD TCPFTSSKLS TFNRHIKIHS NERPHLCHLC LKAFRTVTLL
310 320 330 340 350
RNHVNTHTGT RPHKCRDCDM AFVTSGELVR HRRYKHTYEK PFKCSLCKYA
360 370 380 390 400
SVEASKMKRH IRSHTGERPF QCCQCAYASR DSYKLKRHMR THSGEKPYEC
410 420 430 440 450
PTCHVRFTQS GTMKIHIAQK HGENVPKYEC PHCATIIARK SDLRVHLRNL
460 470 480 490 500
HSQSPEEMKC RYCPAGFHER YALIQHQRTH KNEKKFKCKQ CDYACKQERC
510 520 530 540 550
LKAHMRMHTG EKPFSCLACN KHFRQKQLLT VHLRKYHDPN FVPNLHLCLK
560 570 580 590 600
CDKRFSRWSN LQRHRKKCDP EHETLAPNKD RRPVTRTQAS EGEAGHKEGE
610 620 630
PQCPGEQALG HQGEAAGSQS PDHGLTCEII FNMMDK
Length:636
Mass (Da):73,108
Last modified:February 20, 2007 - v1
Checksum:iBECF76F0C84FA7F0
GO
Isoform 2 (identifier: A2APF3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     598-598: E → EDVATQESATS

Note: No experimental confirmation available.
Show »
Length:646
Mass (Da):74,098
Checksum:i652CDE173C09D872
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti629I → M in AAZ79234 (PubMed:12011441).Curated1
Sequence conflicti629I → M in AAI51027 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_037281598E → EDVATQESATS in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ153171 mRNA Translation: AAZ79234.1
AL928599, AL837509 Genomic DNA Translation: CAM22368.1
AL837509, AL928599 Genomic DNA Translation: CAM24671.1
BC151026 mRNA Translation: AAI51027.1
CCDSiCCDS38349.1 [A2APF3-1]
RefSeqiNP_001074856.1, NM_001081387.2 [A2APF3-1]
XP_017174657.1, XM_017319168.1 [A2APF3-2]
UniGeneiMm.389396

Genome annotation databases

EnsembliENSMUST00000094287; ENSMUSP00000091845; ENSMUSG00000070495 [A2APF3-1]
ENSMUST00000179693; ENSMUSP00000135932; ENSMUSG00000070495 [A2APF3-1]
GeneIDi664799
KEGGimmu:664799
UCSCiuc008odi.1 mouse [A2APF3-1]
uc012cks.1 mouse [A2APF3-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ153171 mRNA Translation: AAZ79234.1
AL928599, AL837509 Genomic DNA Translation: CAM22368.1
AL837509, AL928599 Genomic DNA Translation: CAM24671.1
BC151026 mRNA Translation: AAI51027.1
CCDSiCCDS38349.1 [A2APF3-1]
RefSeqiNP_001074856.1, NM_001081387.2 [A2APF3-1]
XP_017174657.1, XM_017319168.1 [A2APF3-2]
UniGeneiMm.389396

3D structure databases

ProteinModelPortaliA2APF3
SMRiA2APF3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi576678, 2 interactors
DIPiDIP-29209N
IntActiA2APF3, 5 interactors
STRINGi10090.ENSMUSP00000091845

PTM databases

iPTMnetiA2APF3
PhosphoSitePlusiA2APF3

Proteomic databases

MaxQBiA2APF3
PaxDbiA2APF3
PeptideAtlasiA2APF3
PRIDEiA2APF3

Protocols and materials databases

DNASUi664799
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000094287; ENSMUSP00000091845; ENSMUSG00000070495 [A2APF3-1]
ENSMUST00000179693; ENSMUSP00000135932; ENSMUSG00000070495 [A2APF3-1]
GeneIDi664799
KEGGimmu:664799
UCSCiuc008odi.1 mouse [A2APF3-1]
uc012cks.1 mouse [A2APF3-2]

Organism-specific databases

CTDi140690
MGIiMGI:3652571 Ctcfl

Phylogenomic databases

eggNOGiKOG1721 Eukaryota
COG5048 LUCA
GeneTreeiENSGT00890000139371
HOGENOMiHOG000276534
InParanoidiA2APF3
OMAiLRNLHTY
OrthoDBiEOG091G06B4
TreeFamiTF106430

Miscellaneous databases

PROiPR:A2APF3
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000070495 Expressed in 25 organ(s), highest expression level in telencephalon

Family and domain databases

InterProiView protein in InterPro
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PfamiView protein in Pfam
PF00096 zf-C2H2, 4 hits
SMARTiView protein in SMART
SM00355 ZnF_C2H2, 11 hits
SUPFAMiSSF57667 SSF57667, 6 hits
PROSITEiView protein in PROSITE
PS00028 ZINC_FINGER_C2H2_1, 7 hits
PS50157 ZINC_FINGER_C2H2_2, 11 hits
ProtoNetiSearch...

Entry informationi

Entry nameiCTCFL_MOUSE
AccessioniPrimary (citable) accession number: A2APF3
Secondary accession number(s): B9EKP6, Q3Y6S0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: February 20, 2007
Last modified: November 7, 2018
This is version 106 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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