Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Targeting protein for Xklp2

Gene

Tpx2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Spindle assembly factor required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules. Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation. TPX2 is inactivated upon binding to importin-alpha. At the onset of mitosis, GOLGA2 interacts with importin-alpha, liberating TPX2 from importin-alpha, allowing TPX2 to activates AURKA kinase and stimulates local microtubule nucleation.By similarity1 Publication

GO - Molecular functioni

  • importin-alpha family protein binding Source: UniProtKB
  • protein kinase binding Source: UniProtKB

GO - Biological processi

Keywordsi

Biological processApoptosis, Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiR-MMU-6804756 Regulation of TP53 Activity through Phosphorylation
R-MMU-8854518 AURKA Activation by TPX2

Names & Taxonomyi

Protein namesi
Recommended name:
Targeting protein for Xklp2
Gene namesi
Name:Tpx2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1919369 Tpx2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi8 – 11YSFD → ASAA: Abolishes interaction with AURKA. 1 Publication4

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003931121 – 745Targeting protein for Xklp2Add BLAST745

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei72PhosphothreonineCombined sources1
Modified residuei121PhosphoserineBy similarity1
Modified residuei125PhosphoserineBy similarity1
Modified residuei128N6-acetyllysineCombined sources1
Modified residuei294PhosphoserineBy similarity1
Modified residuei307N6-acetyllysineBy similarity1
Modified residuei312PhosphoserineBy similarity1
Modified residuei340PhosphothreonineBy similarity1
Modified residuei359PhosphoserineBy similarity1
Modified residuei369PhosphothreonineBy similarity1
Modified residuei375N6-acetyllysineCombined sources1
Cross-linki477Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei486PhosphoserineCombined sources1
Cross-linki500Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki640Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei737PhosphoserineCombined sources1
Cross-linki739Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiA2APB8
MaxQBiA2APB8
PaxDbiA2APB8
PeptideAtlasiA2APB8
PRIDEiA2APB8

PTM databases

iPTMnetiA2APB8
PhosphoSitePlusiA2APB8

Expressioni

Gene expression databases

BgeeiENSMUSG00000027469 Expressed in 194 organ(s), highest expression level in cumulus cell
GenevisibleiA2APB8 MM

Interactioni

Subunit structurei

Interacts with AURKA (PubMed:18663142). Interacts with importin-alpha; leading to inactivate TPX2 (By similarity). Interacts with HNRNPU; this interaction recruits HNRNPU to spindle microtubules (MTs) (By similarity).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi215163, 53 interactors
IntActiA2APB8, 51 interactors
MINTiA2APB8
STRINGi10090.ENSMUSP00000028969

Structurei

3D structure databases

ProteinModelPortaliA2APB8
SMRiA2APB8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TPX2 family.Curated

Phylogenomic databases

eggNOGiENOG410IIEY Eukaryota
ENOG4110SQ6 LUCA
GeneTreeiENSGT00390000009842
HOGENOMiHOG000231739
HOVERGENiHBG057334
InParanoidiA2APB8
KOiK16812
OMAiAPTDFIN
OrthoDBiEOG091G0MIV
PhylomeDBiA2APB8
TreeFamiTF328997

Family and domain databases

InterProiView protein in InterPro
IPR015128 Aurora-A-bd
IPR027329 TPX2_C
IPR027330 TPX2_central_dom
IPR009675 TPX2_fam
PANTHERiPTHR14326 PTHR14326, 1 hit
PfamiView protein in Pfam
PF09041 Aurora-A_bind, 1 hit
PF06886 TPX2, 1 hit
PF12214 TPX2_importin, 1 hit

Sequencei

Sequence statusi: Complete.

A2APB8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSQVPTTYSF DAPTDFINFS SLDAEEDTEN IDSWFDEKAN LENKFLRQRG
60 70 80 90 100
IGEPFQGKNS LRKAKLQQGF VTPLKAVDNT YHKETEKENL QKQSIPSNDC
110 120 130 140 150
SSLDAKRAVS GNTPVQPQRR SIRLSAQKDL EQKEKNHVAS VEMKAKRCVA
160 170 180 190 200
PATDCPPQKR MKVSHKKKLE EEEEGSAPAT SRKNERETLE KAKGKHTVPG
210 220 230 240 250
VPPAREKVLK STEEQEIEKR LRMQQEVVEL RRKNEEFKKL ALAGPGQPVK
260 270 280 290 300
KSTSQVTKTV DFHFLTDERI KQHPKNQEEY KEVNFMSELR KHSSTPARGT
310 320 330 340 350
RGCTIIKPFN LSKGKKRTFD EAASTYVPIA QQVEAFHKRT PNRYHLRNKK
360 370 380 390 400
DESLLPSKSV NKIARDPQTP ILQTKYRTRA VTCKSTAEQE AEELEKLQQY
410 420 430 440 450
KFKARELDPR IFESGPILPK RAPVKPPTQP VGFDLEIEKR IHERESKKKT
460 470 480 490 500
EDEQFEFHSR PCPTKILEDV VGVPEKKVIP ATVPKSPVFA LKNRIRVPIK
510 520 530 540 550
DEEEEKPVVI KAQPVPHYGV PYKPHIAEAR NVEVCPFSFD TRDKERQLQK
560 570 580 590 600
EKKIKEMQKG EVPKFKALPV PHFDTINLPE KKVKNVTQAE PFSLETDKRG
610 620 630 640 650
AYKAEMWKHQ LEEEQKQQKD AACFKARPNT VIFQEPFVPK KEKKSLAENP
660 670 680 690 700
SGSLVQEPFQ LATERRAKER QELEKKMAEV EAWKLQQLEE VRQQEEEQQK
710 720 730 740
EELARLRKEL VHKANPIRKY AAVEVKSSEL PLTVPVSPKF STRFQ
Length:745
Mass (Da):85,894
Last modified:February 20, 2007 - v1
Checksum:i4E60692BC52DAE34
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti165H → D in AAH60619 (PubMed:15489334).Curated1
Sequence conflicti453E → K in BAE32280 (PubMed:16141072).Curated1
Sequence conflicti509V → L in BAE32280 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK153957 mRNA Translation: BAE32280.1
AL833801 Genomic DNA Translation: CAM23677.1
CH466551 Genomic DNA Translation: EDL05994.1
BC060619 mRNA Translation: AAH60619.1
CCDSiCCDS16900.1
RefSeqiNP_001135447.1, NM_001141975.1
NP_001135448.1, NM_001141976.1
NP_001135449.1, NM_001141977.1
NP_001135450.1, NM_001141978.1
NP_082385.3, NM_028109.4
XP_006500276.1, XM_006500213.2
XP_006500277.1, XM_006500214.2
XP_006500278.1, XM_006500215.2
UniGeneiMm.407737

Genome annotation databases

EnsembliENSMUST00000028969; ENSMUSP00000028969; ENSMUSG00000027469
ENSMUST00000109816; ENSMUSP00000105441; ENSMUSG00000027469
ENSMUST00000164120; ENSMUSP00000128888; ENSMUSG00000027469
ENSMUST00000178997; ENSMUSP00000136457; ENSMUSG00000027469
GeneIDi72119
KEGGimmu:72119
UCSCiuc008ngo.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK153957 mRNA Translation: BAE32280.1
AL833801 Genomic DNA Translation: CAM23677.1
CH466551 Genomic DNA Translation: EDL05994.1
BC060619 mRNA Translation: AAH60619.1
CCDSiCCDS16900.1
RefSeqiNP_001135447.1, NM_001141975.1
NP_001135448.1, NM_001141976.1
NP_001135449.1, NM_001141977.1
NP_001135450.1, NM_001141978.1
NP_082385.3, NM_028109.4
XP_006500276.1, XM_006500213.2
XP_006500277.1, XM_006500214.2
XP_006500278.1, XM_006500215.2
UniGeneiMm.407737

3D structure databases

ProteinModelPortaliA2APB8
SMRiA2APB8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi215163, 53 interactors
IntActiA2APB8, 51 interactors
MINTiA2APB8
STRINGi10090.ENSMUSP00000028969

PTM databases

iPTMnetiA2APB8
PhosphoSitePlusiA2APB8

Proteomic databases

EPDiA2APB8
MaxQBiA2APB8
PaxDbiA2APB8
PeptideAtlasiA2APB8
PRIDEiA2APB8

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028969; ENSMUSP00000028969; ENSMUSG00000027469
ENSMUST00000109816; ENSMUSP00000105441; ENSMUSG00000027469
ENSMUST00000164120; ENSMUSP00000128888; ENSMUSG00000027469
ENSMUST00000178997; ENSMUSP00000136457; ENSMUSG00000027469
GeneIDi72119
KEGGimmu:72119
UCSCiuc008ngo.2 mouse

Organism-specific databases

CTDi22974
MGIiMGI:1919369 Tpx2

Phylogenomic databases

eggNOGiENOG410IIEY Eukaryota
ENOG4110SQ6 LUCA
GeneTreeiENSGT00390000009842
HOGENOMiHOG000231739
HOVERGENiHBG057334
InParanoidiA2APB8
KOiK16812
OMAiAPTDFIN
OrthoDBiEOG091G0MIV
PhylomeDBiA2APB8
TreeFamiTF328997

Enzyme and pathway databases

ReactomeiR-MMU-6804756 Regulation of TP53 Activity through Phosphorylation
R-MMU-8854518 AURKA Activation by TPX2

Miscellaneous databases

ChiTaRSiTpx2 mouse
PROiPR:A2APB8
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027469 Expressed in 194 organ(s), highest expression level in cumulus cell
GenevisibleiA2APB8 MM

Family and domain databases

InterProiView protein in InterPro
IPR015128 Aurora-A-bd
IPR027329 TPX2_C
IPR027330 TPX2_central_dom
IPR009675 TPX2_fam
PANTHERiPTHR14326 PTHR14326, 1 hit
PfamiView protein in Pfam
PF09041 Aurora-A_bind, 1 hit
PF06886 TPX2, 1 hit
PF12214 TPX2_importin, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTPX2_MOUSE
AccessioniPrimary (citable) accession number: A2APB8
Secondary accession number(s): Q3U500, Q6P9S6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 23, 2010
Last sequence update: February 20, 2007
Last modified: November 7, 2018
This is version 90 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again