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Protein

Protein AF-9

Gene

Mllt3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Chromatin reader component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. Specifically recognizes and binds acylated histone H3, with a marked preference for histone H3 that is crotonylated. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. Recognizes and binds histone H3 crotonylated at 'Lys-9' (H3K9cr), and with slightly lower affinity histone H3 crotonylated at 'Lys-18' (H3K18cr). Also recognizes and binds histone H3 acetylated at 'Lys-9' (H3K9ac), but with lower affinity than crotonylated histone H3. In the SEC complex, MLLT3 is required to recruit the complex to crotonylated histones.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-112382 Formation of RNA Pol II elongation complex
R-MMU-674695 RNA Polymerase II Pre-transcription Events
R-MMU-75955 RNA Polymerase II Transcription Elongation

Names & Taxonomyi

Protein namesi
Recommended name:
Protein AF-9
Alternative name(s):
Myeloid/lymphoid or mixed-lineage leukemia translocated to chromosome 3 protein homolog
Gene namesi
Name:Mllt3
Synonyms:Af9
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1917372 Mllt3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003051241 – 569Protein AF-9Add BLAST569

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei289PhosphoserineBy similarity1
Modified residuei295PhosphoserineBy similarity1
Cross-linki340Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei413PhosphoserineCombined sources1
Modified residuei420PhosphoserineBy similarity1
Modified residuei484PhosphoserineBy similarity1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiA2AM29
MaxQBiA2AM29
PaxDbiA2AM29
PeptideAtlasiA2AM29
PRIDEiA2AM29

PTM databases

iPTMnetiA2AM29
PhosphoSitePlusiA2AM29
SwissPalmiA2AM29

Expressioni

Tissue specificityi

Ubiquitously expressed. Strong expression in the spleen.1 Publication

Gene expression databases

BgeeiENSMUSG00000028496 Expressed in 316 organ(s), highest expression level in pancreas
ExpressionAtlasiA2AM29 baseline and differential
GenevisibleiA2AM29 MM

Interactioni

Subunit structurei

Component of the super elongation complex (SEC), at least composed of EAF1, EAF2, CDK9, MLLT3/AF9, AFF (AFF1 or AFF4), the P-TEFb complex and ELL (ELL, ELL2 or ELL3) (By similarity). Interacts with BCOR (By similarity). Interacts with CBX8 (PubMed:11439343). Interacts with ALKBH4 (By similarity).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi213873, 8 interactors
DIPiDIP-60099N
IntActiA2AM29, 4 interactors
STRINGi10090.ENSMUSP00000077232

Structurei

3D structure databases

ProteinModelPortaliA2AM29
SMRiA2AM29
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 112YEATSPROSITE-ProRule annotationAdd BLAST105

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi296 – 301Nuclear localization signalSequence analysis6

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi149 – 194Poly-SerAdd BLAST46
Compositional biasi384 – 392Poly-Ser9
Compositional biasi467 – 470Poly-Pro4

Domaini

The YEATS domain specifically recognizes and binds acylated histones, with a marked preference histones that are crotonylated. Also binds histone H3 acetylated at 'Lys-9' (H3K9ac), but with lower affinity.By similarity

Phylogenomic databases

eggNOGiKOG3149 Eukaryota
COG5033 LUCA
GeneTreeiENSGT00440000039936
HOGENOMiHOG000033834
HOVERGENiHBG004191
InParanoidiA2AM29
KOiK15187
OMAiMSKEPKS
OrthoDBiEOG091G06LN
PhylomeDBiA2AM29
TreeFamiTF314586

Family and domain databases

Gene3Di2.60.40.1970, 1 hit
InterProiView protein in InterPro
IPR038704 YEAST_sf
IPR005033 YEATS
PANTHERiPTHR23195 PTHR23195, 1 hit
PfamiView protein in Pfam
PF03366 YEATS, 1 hit
PROSITEiView protein in PROSITE
PS51037 YEATS, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All

A2AM29-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MASSCAVQVK LELGHRAQVR KKPTVEGFTH DWMVFVRGPE HSNIQHFVEK
60 70 80 90 100
VVFHLHESFP RPKRVCKDPP YKVEESGYAG FILPIEVYFK NKEEPKKVRF
110 120 130 140 150
DYDLFLHLEG HPPVNHLRCE KLTFNNPTED FRRKLLKAGG DPNRSIHTSS
160 170 180 190 200
SSSSSSSSSS SSSSSSSSSS SSSSSSSSSS SSSSSSSSSS TSFSKPHKLM
210 220 230 240 250
KEHKEKPSKD SREHKSAFKE PSRDHNKSSK DSSKKPKENK PLKEEKIVPK
260 270 280 290 300
MAFKEPKPMS KEPKADSNLL TVTSGQQDKK APSKRPPASD SEELSAKKRK
310 320 330 340 350
KSSSEALFKS FSSAPPLILT CSADKKQIKD KSHVKMGKVK IESETSEKKK
360 370 380 390 400
SMLPPFDDIV DPNDSDVEEN MSSKSDSEQP SPASSSSSSS SSFTPSQTRQ
410 420 430 440 450
QGPLRSIMKD LHSDDNEEES DEAEDNDNDS EMERPVNRGG SRSRRVSLSD
460 470 480 490 500
GSDSESSSAS SPLHHEPPPP LLKTNNNQIL EVKSPIKQSK SDKQIKNGEC
510 520 530 540 550
DKAYLDELVE LHRRLMTLRE RHILQQIVNL IEETGHFHIT NTTFDFDLCS
560
LDKTTVRKLQ SYLETSGTS
Length:569
Mass (Da):63,375
Last modified:February 20, 2007 - v1
Checksum:i120A0604B13675EC
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9Q3B8E9Q3B8_MOUSE
Protein AF-9
Mllt3
73Annotation score:
Q3UIA3Q3UIA3_MOUSE
Protein AF-9
Mllt3
162Annotation score:
Q9D2P1Q9D2P1_MOUSE
Protein AF-9
Mllt3
138Annotation score:
E9PXP8E9PXP8_MOUSE
Protein AF-9
Mllt3
54Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti157S → G in AAK28536 (PubMed:11439343).Curated1
Sequence conflicti164S → G in AAK28536 (PubMed:11439343).Curated1
Sequence conflicti172S → N in AAK28536 (PubMed:11439343).Curated1
Sequence conflicti176S → G in AAK28536 (PubMed:11439343).Curated1
Sequence conflicti179 – 190Missing in AAH21420 (PubMed:15489334).CuratedAdd BLAST12
Sequence conflicti352M → T in AAH21420 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL831756, AL806533, AL929524 Genomic DNA Translation: CAM20202.1
AL929524, AL806533, AL831756 Genomic DNA Translation: CAM24441.1
AL806533, AL831756, AL929524 Genomic DNA Translation: CAM25293.1
BC021420 mRNA Translation: AAH21420.1
AF333960 mRNA Translation: AAK28536.1
CCDSiCCDS38796.1
RefSeqiNP_081602.3, NM_027326.3
UniGeneiMm.288898
Mm.472285

Genome annotation databases

EnsembliENSMUST00000078090; ENSMUSP00000077232; ENSMUSG00000028496
GeneIDi70122
KEGGimmu:70122
UCSCiuc008tmp.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL831756, AL806533, AL929524 Genomic DNA Translation: CAM20202.1
AL929524, AL806533, AL831756 Genomic DNA Translation: CAM24441.1
AL806533, AL831756, AL929524 Genomic DNA Translation: CAM25293.1
BC021420 mRNA Translation: AAH21420.1
AF333960 mRNA Translation: AAK28536.1
CCDSiCCDS38796.1
RefSeqiNP_081602.3, NM_027326.3
UniGeneiMm.288898
Mm.472285

3D structure databases

ProteinModelPortaliA2AM29
SMRiA2AM29
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi213873, 8 interactors
DIPiDIP-60099N
IntActiA2AM29, 4 interactors
STRINGi10090.ENSMUSP00000077232

PTM databases

iPTMnetiA2AM29
PhosphoSitePlusiA2AM29
SwissPalmiA2AM29

Proteomic databases

EPDiA2AM29
MaxQBiA2AM29
PaxDbiA2AM29
PeptideAtlasiA2AM29
PRIDEiA2AM29

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000078090; ENSMUSP00000077232; ENSMUSG00000028496
GeneIDi70122
KEGGimmu:70122
UCSCiuc008tmp.1 mouse

Organism-specific databases

CTDi4300
MGIiMGI:1917372 Mllt3

Phylogenomic databases

eggNOGiKOG3149 Eukaryota
COG5033 LUCA
GeneTreeiENSGT00440000039936
HOGENOMiHOG000033834
HOVERGENiHBG004191
InParanoidiA2AM29
KOiK15187
OMAiMSKEPKS
OrthoDBiEOG091G06LN
PhylomeDBiA2AM29
TreeFamiTF314586

Enzyme and pathway databases

ReactomeiR-MMU-112382 Formation of RNA Pol II elongation complex
R-MMU-674695 RNA Polymerase II Pre-transcription Events
R-MMU-75955 RNA Polymerase II Transcription Elongation

Miscellaneous databases

ChiTaRSiMllt3 mouse
PROiPR:A2AM29
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028496 Expressed in 316 organ(s), highest expression level in pancreas
ExpressionAtlasiA2AM29 baseline and differential
GenevisibleiA2AM29 MM

Family and domain databases

Gene3Di2.60.40.1970, 1 hit
InterProiView protein in InterPro
IPR038704 YEAST_sf
IPR005033 YEATS
PANTHERiPTHR23195 PTHR23195, 1 hit
PfamiView protein in Pfam
PF03366 YEATS, 1 hit
PROSITEiView protein in PROSITE
PS51037 YEATS, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAF9_MOUSE
AccessioniPrimary (citable) accession number: A2AM29
Secondary accession number(s): Q8VDR6, Q99MK4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: February 20, 2007
Last modified: September 12, 2018
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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