UniProtKB - A2ALU4 (SHRM2_MOUSE)
Protein Shroom2
Shroom2
Functioni
May be involved in endothelial cell morphology changes during cell spreading. In the retinal pigment epithelium, may regulate the biogenesis of melanosomes and promote their association with the apical cell surface by inducing gamma-tubulin redistribution.
2 PublicationsGO - Molecular functioni
- actin binding Source: UniProtKB
- actin filament binding Source: UniProtKB
- beta-catenin binding Source: UniProtKB
- protein domain specific binding Source: MGI
GO - Biological processi
- actin filament organization Source: GO_Central
- apical protein localization Source: UniProtKB
- brain development Source: UniProtKB
- camera-type eye development Source: UniProtKB
- camera-type eye morphogenesis Source: UniProtKB
- cell-cell junction maintenance Source: MGI
- cell migration Source: UniProtKB
- cellular pigment accumulation Source: UniProtKB
- ear development Source: UniProtKB
- establishment of melanosome localization Source: UniProtKB
- eye pigment granule organization Source: UniProtKB
- lens morphogenesis in camera-type eye Source: UniProtKB
- melanosome organization Source: UniProtKB
- negative regulation of actin filament depolymerization Source: MGI
Keywordsi
Molecular function | Actin-binding, Developmental protein |
Names & Taxonomyi
Protein namesi | Recommended name: Protein Shroom2Alternative name(s): Protein Apxl |
Gene namesi | Name:Shroom2 Synonyms:Apxl |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:107194, Shroom2 |
VEuPathDBi | HostDB:ENSMUSG00000045180 |
Subcellular locationi
Cytoskeleton
- cytoskeleton 1 Publication
Plasma membrane
- Apical cell membrane 1 Publication
Other locations
- tight junction 1 Publication
Note: Associates with cortical F-actin.
Cytoskeleton
- cytoskeleton Source: UniProtKB
- microtubule Source: UniProtKB-KW
Plasma Membrane
- apical plasma membrane Source: UniProtKB
- plasma membrane Source: UniProtKB
Other locations
- adherens junction Source: UniProtKB
- apical junction complex Source: MGI
- bicellular tight junction Source: UniProtKB
- cell cortex Source: MGI
- cell-cell junction Source: MGI
- cytoplasm Source: MGI
- neuronal cell body Source: MGI
Keywords - Cellular componenti
Cell junction, Cell membrane, Cytoplasm, Cytoskeleton, Membrane, Microtubule, Tight junctionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000286063 | 1 – 1481 | Protein Shroom2Add BLAST | 1481 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 236 | PhosphoserineCombined sources | 1 | |
Modified residuei | 318 | PhosphoserineCombined sources | 1 | |
Modified residuei | 330 | PhosphoserineCombined sources | 1 | |
Modified residuei | 935 | PhosphoserineCombined sources | 1 | |
Modified residuei | 959 | PhosphoserineCombined sources | 1 | |
Modified residuei | 960 | PhosphoserineCombined sources | 1 | |
Modified residuei | 962 | PhosphoserineCombined sources | 1 | |
Modified residuei | 963 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 1041 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1043 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1165 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | A2ALU4 |
PaxDbi | A2ALU4 |
PeptideAtlasi | A2ALU4 |
PRIDEi | A2ALU4 |
ProteomicsDBi | 257162 [A2ALU4-1] 257163 [A2ALU4-2] |
PTM databases
iPTMneti | A2ALU4 |
PhosphoSitePlusi | A2ALU4 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000045180, Expressed in central nervous system and 253 other tissues |
ExpressionAtlasi | A2ALU4, baseline and differential |
Genevisiblei | A2ALU4, MM |
Interactioni
Subunit structurei
Interacts with F-actin.
1 PublicationGO - Molecular functioni
- actin binding Source: UniProtKB
- actin filament binding Source: UniProtKB
- beta-catenin binding Source: UniProtKB
- protein domain specific binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 225549, 8 interactors |
STRINGi | 10090.ENSMUSP00000098701 |
Miscellaneous databases
RNActi | A2ALU4, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 26 – 108 | PDZPROSITE-ProRule annotationAdd BLAST | 83 | |
Domaini | 705 – 807 | ASD1PROSITE-ProRule annotationAdd BLAST | 103 | |
Domaini | 1185 – 1476 | ASD2PROSITE-ProRule annotationAdd BLAST | 292 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 20 | DisorderedSequence analysisAdd BLAST | 20 | |
Regioni | 189 – 234 | DisorderedSequence analysisAdd BLAST | 46 | |
Regioni | 250 – 299 | DisorderedSequence analysisAdd BLAST | 50 | |
Regioni | 430 – 501 | DisorderedSequence analysisAdd BLAST | 72 | |
Regioni | 612 – 643 | DisorderedSequence analysisAdd BLAST | 32 | |
Regioni | 717 – 760 | DisorderedSequence analysisAdd BLAST | 44 | |
Regioni | 944 – 983 | DisorderedSequence analysisAdd BLAST | 40 | |
Regioni | 997 – 1051 | DisorderedSequence analysisAdd BLAST | 55 | |
Regioni | 1086 – 1112 | DisorderedSequence analysisAdd BLAST | 27 | |
Regioni | 1139 – 1182 | DisorderedSequence analysisAdd BLAST | 44 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1 – 18 | Basic and acidic residuesSequence analysisAdd BLAST | 18 | |
Compositional biasi | 189 – 203 | Polar residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 213 – 234 | Polar residuesSequence analysisAdd BLAST | 22 | |
Compositional biasi | 251 – 267 | Polar residuesSequence analysisAdd BLAST | 17 | |
Compositional biasi | 430 – 445 | Polar residuesSequence analysisAdd BLAST | 16 | |
Compositional biasi | 737 – 753 | Polar residuesSequence analysisAdd BLAST | 17 | |
Compositional biasi | 953 – 970 | Polar residuesSequence analysisAdd BLAST | 18 | |
Compositional biasi | 997 – 1048 | Polar residuesSequence analysisAdd BLAST | 52 | |
Compositional biasi | 1147 – 1162 | Pro residuesSequence analysisAdd BLAST | 16 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG502QUU2, Eukaryota |
GeneTreei | ENSGT00940000155212 |
HOGENOMi | CLU_003220_0_0_1 |
InParanoidi | A2ALU4 |
OrthoDBi | 29607at2759 |
Family and domain databases
Gene3Di | 2.30.42.10, 1 hit |
InterProi | View protein in InterPro IPR014800, ASD1_dom IPR014799, ASD2_dom IPR001478, PDZ IPR036034, PDZ_sf IPR027686, Shroom2 IPR027685, Shroom_fam |
PANTHERi | PTHR15012, PTHR15012, 2 hits PTHR15012:SF8, PTHR15012:SF8, 2 hits |
Pfami | View protein in Pfam PF08688, ASD1, 1 hit PF08687, ASD2, 1 hit PF00595, PDZ, 1 hit |
SMARTi | View protein in SMART SM00228, PDZ, 1 hit |
SUPFAMi | SSF50156, SSF50156, 1 hit |
PROSITEi | View protein in PROSITE PS51306, ASD1, 1 hit PS51307, ASD2, 1 hit PS50106, PDZ, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MEGAEPRARP ERLAEAEAPA TDGVRLVEVQ LSGGAPWGFT LKGGREHGEP
60 70 80 90 100
LVITKIEEGS KAAAVDKLLA GDEIVAINDV SLSGFRQEAI CLVKGSHKTL
110 120 130 140 150
KLVVKRKSDP SWRPHSWHAT KYFDVHPEPA ASLFLNTSGS PSWKSQHQAS
160 170 180 190 200
SSSHDLSGSW EHTSLQRTSD HFSSMGSIDS LDHSSQLYPS GHLSSAKSNS
210 220 230 240 250
SIDHLGGHSK RDSAYGSFST CSSTPDHTLP KADASSTENI LYKVGLWEAS
260 270 280 290 300
RPGSSRQSQS TGDPQGLQDR PSCFIPRVPG NSSKSPRPED NVEPKIATHG
310 320 330 340 350
RSNFGPVWYV PDKKKAPSPP PLGLPLRSDS FSVAARGHEK ARGPPFSDLA
360 370 380 390 400
SMQHFITLPH VQPRGDHRME TTDRQWKLTH LSSGKEIGNV GYQSEGHLDC
410 420 430 440 450
RWLCSDDRAG RPSGPPGRLQ FSDVHFLKSY HGSQHQQQCS DESPRAPSSP
460 470 480 490 500
RELLHITPGG GLQEPPEPSQ DDNPTQVRWP GSAHQKLDDR GRSHYFPGSL
510 520 530 540 550
RQPVQGSAQV VIPRGDYWHS DTTPVDLEYP LLRPVGQRTY LQQHEETPAS
560 570 580 590 600
HEKEGYHQLN AGIEGCCSGI QEPPRASRTV RTGLQCPSND FKLVDGESGR
610 620 630 640 650
ISRQRTPMLH SLTQDGTWRP GNSKDCGNDK PPLFDAQVGK PTRRSDRFAT
660 670 680 690 700
TLRNEIQMRR AKLQKSKSTV TLAGDSEAED CAGDWRADVG AVPEGSFPST
710 720 730 740 750
YKEHLKEAQT RVLKATSFQR RDLDPTPADQ YSGPSEHRTF DHSASSSLSS
760 770 780 790 800
FPGEPDSAPR FCETGLAKAP SSGVGVPHVL RIGGRKRFTA EQKLKSYSEP
810 820 830 840 850
EKINEVGLSG DHRPHPTVRT PEDTVGTFAD RWKFFEETSK SLLQKAGHRQ
860 870 880 890 900
VHCGLPREKA ERPQTGHHEC ESTEPWFQKR SLATSCGEIL SDRKVEKASE
910 920 930 940 950
KLNPPRRLGT FAEYQASWKE QKKPLEARSS GRYHSADDIL DAGLDQQQRP
960 970 980 990 1000
QYIHERSRSS PSTDHYSQEV PVEPNRQAED SGDHKEAILC TLQAEEGCSA
1010 1020 1030 1040 1050
PSAQPQDSQH VNEDTTFPQP ETQLSSKCQH LQTSAMETSR SPSPQFAPQK
1060 1070 1080 1090 1100
LTDKPPLLIH EDNSARIERV MDNNTTVKMV PIKIVHSESQ PEKESRQSLA
1110 1120 1130 1140 1150
CPAELPPLPS GLERDQIKTL STSEQCYSRF CVYTRQEVEA PHRARPPEPR
1160 1170 1180 1190 1200
PPSTPAPPVR DSCSSPPSLN YGKAKEKTMD DLKSEELARE IVGKDKSLAD
1210 1220 1230 1240 1250
ILDPSVKIKT TMDLMEGIFP KDEYLLEEAQ QRRKLLPKVP LPRVTEDKKQ
1260 1270 1280 1290 1300
DPGMPGVVSL ATNSTYYSTS APKAELLIKM KDLQEPEEYS AGDLDHDLSV
1310 1320 1330 1340 1350
KKQELIDSIS RKLQVLREAR ESLLEDIQAN NALGDEVEAI VKDVCKPNEF
1360 1370 1380 1390 1400
DKFRMFIGDL DKVVNLLLSL SGRLARVENA LNNLDDNPSP GDRQSLLEKQ
1410 1420 1430 1440 1450
RVLTQQHEDA KELKENLDRR ERIVFDILAT YLSEENLADY EHFVKMKSAL
1460 1470 1480
IIEQRELEDK IHLGEEQLKC LFDSLQPERS K
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA7TU71 | A7TU71_MOUSE | Protein Shroom2 | Shroom2 | 1,487 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 274 | F → S in ABD19518 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 458 | P → S in ABD19518 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 603 | R → C in ABD19518 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 1012 | N → K in BAC27781 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 1100 | A → S in ABD19518 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 1227 | E → K in ABD19518 (PubMed:16141072).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_024964 | 1 – 1035 | Missing in isoform 2. 1 PublicationAdd BLAST | 1035 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DQ358971 mRNA Translation: ABD19518.1 Frameshift. AK029338 mRNA Translation: BAC26404.1 Different initiation. AK032256 mRNA Translation: BAC27781.1 Different initiation. AK049850 mRNA Translation: BAC33957.1 AL805960 Genomic DNA No translation available. AL807777 Genomic DNA No translation available. |
CCDSi | CCDS72455.1 [A2ALU4-1] |
RefSeqi | NP_001277613.1, NM_001290684.1 [A2ALU4-1] NP_001277614.1, NM_001290685.1 NP_001277615.1, NM_001290686.1 [A2ALU4-2] NP_001277616.1, NM_001290687.1 [A2ALU4-2] NP_766029.2, NM_172441.3 XP_011246074.1, XM_011247772.1 XP_011246075.1, XM_011247773.1 XP_011246076.1, XM_011247774.2 [A2ALU4-2] XP_011246077.1, XM_011247775.2 [A2ALU4-2] XP_011246078.1, XM_011247776.2 XP_011246079.1, XM_011247777.2 [A2ALU4-2] |
Genome annotation databases
Ensembli | ENSMUST00000062317; ENSMUSP00000057500; ENSMUSG00000045180 [A2ALU4-1] |
GeneIDi | 110380 |
KEGGi | mmu:110380 |
UCSCi | uc009uqj.2, mouse [A2ALU4-2] uc009uqm.2, mouse [A2ALU4-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DQ358971 mRNA Translation: ABD19518.1 Frameshift. AK029338 mRNA Translation: BAC26404.1 Different initiation. AK032256 mRNA Translation: BAC27781.1 Different initiation. AK049850 mRNA Translation: BAC33957.1 AL805960 Genomic DNA No translation available. AL807777 Genomic DNA No translation available. |
CCDSi | CCDS72455.1 [A2ALU4-1] |
RefSeqi | NP_001277613.1, NM_001290684.1 [A2ALU4-1] NP_001277614.1, NM_001290685.1 NP_001277615.1, NM_001290686.1 [A2ALU4-2] NP_001277616.1, NM_001290687.1 [A2ALU4-2] NP_766029.2, NM_172441.3 XP_011246074.1, XM_011247772.1 XP_011246075.1, XM_011247773.1 XP_011246076.1, XM_011247774.2 [A2ALU4-2] XP_011246077.1, XM_011247775.2 [A2ALU4-2] XP_011246078.1, XM_011247776.2 XP_011246079.1, XM_011247777.2 [A2ALU4-2] |
3D structure databases
AlphaFoldDBi | A2ALU4 |
SMRi | A2ALU4 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 225549, 8 interactors |
STRINGi | 10090.ENSMUSP00000098701 |
PTM databases
iPTMneti | A2ALU4 |
PhosphoSitePlusi | A2ALU4 |
Proteomic databases
MaxQBi | A2ALU4 |
PaxDbi | A2ALU4 |
PeptideAtlasi | A2ALU4 |
PRIDEi | A2ALU4 |
ProteomicsDBi | 257162 [A2ALU4-1] 257163 [A2ALU4-2] |
Protocols and materials databases
Antibodypediai | 23655, 175 antibodies from 26 providers |
Genome annotation databases
Ensembli | ENSMUST00000062317; ENSMUSP00000057500; ENSMUSG00000045180 [A2ALU4-1] |
GeneIDi | 110380 |
KEGGi | mmu:110380 |
UCSCi | uc009uqj.2, mouse [A2ALU4-2] uc009uqm.2, mouse [A2ALU4-1] |
Organism-specific databases
CTDi | 357 |
MGIi | MGI:107194, Shroom2 |
VEuPathDBi | HostDB:ENSMUSG00000045180 |
Phylogenomic databases
eggNOGi | ENOG502QUU2, Eukaryota |
GeneTreei | ENSGT00940000155212 |
HOGENOMi | CLU_003220_0_0_1 |
InParanoidi | A2ALU4 |
OrthoDBi | 29607at2759 |
Miscellaneous databases
BioGRID-ORCSi | 110380, 4 hits in 72 CRISPR screens |
ChiTaRSi | Shroom2, mouse |
PROi | PR:A2ALU4 |
RNActi | A2ALU4, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000045180, Expressed in central nervous system and 253 other tissues |
ExpressionAtlasi | A2ALU4, baseline and differential |
Genevisiblei | A2ALU4, MM |
Family and domain databases
Gene3Di | 2.30.42.10, 1 hit |
InterProi | View protein in InterPro IPR014800, ASD1_dom IPR014799, ASD2_dom IPR001478, PDZ IPR036034, PDZ_sf IPR027686, Shroom2 IPR027685, Shroom_fam |
PANTHERi | PTHR15012, PTHR15012, 2 hits PTHR15012:SF8, PTHR15012:SF8, 2 hits |
Pfami | View protein in Pfam PF08688, ASD1, 1 hit PF08687, ASD2, 1 hit PF00595, PDZ, 1 hit |
SMARTi | View protein in SMART SM00228, PDZ, 1 hit |
SUPFAMi | SSF50156, SSF50156, 1 hit |
PROSITEi | View protein in PROSITE PS51306, ASD1, 1 hit PS51307, ASD2, 1 hit PS50106, PDZ, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SHRM2_MOUSE | |
Accessioni | A2ALU4Primary (citable) accession number: A2ALU4 Secondary accession number(s): Q2EY23 Q8CDZ0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 2007 |
Last sequence update: | February 20, 2007 | |
Last modified: | May 25, 2022 | |
This is version 106 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families