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Protein

Protein Shroom2

Gene

Shroom2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May be involved in endothelial cell morphology changes during cell spreading. In the retinal pigment epithelium, may regulate the biogenesis of melanosomes and promote their association with the apical cell surface by inducing gamma-tubulin redistribution.2 Publications

GO - Molecular functioni

  • actin binding Source: UniProtKB
  • actin filament binding Source: UniProtKB
  • beta-catenin binding Source: UniProtKB
  • protein domain specific binding Source: MGI

GO - Biological processi

Keywordsi

Molecular functionActin-binding, Developmental protein

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Shroom2
Alternative name(s):
Protein Apxl
Gene namesi
Name:Shroom2
Synonyms:Apxl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:107194 Shroom2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Cytoskeleton, Membrane, Microtubule, Tight junction

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002860631 – 1481Protein Shroom2Add BLAST1481

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei236PhosphoserineCombined sources1
Modified residuei318PhosphoserineCombined sources1
Modified residuei330PhosphoserineCombined sources1
Modified residuei935PhosphoserineCombined sources1
Modified residuei959PhosphoserineCombined sources1
Modified residuei960PhosphoserineCombined sources1
Modified residuei962PhosphoserineCombined sources1
Modified residuei963PhosphothreonineCombined sources1
Modified residuei1041PhosphoserineBy similarity1
Modified residuei1043PhosphoserineBy similarity1
Modified residuei1165PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiA2ALU4
PaxDbiA2ALU4
PeptideAtlasiA2ALU4
PRIDEiA2ALU4

PTM databases

iPTMnetiA2ALU4
PhosphoSitePlusiA2ALU4

Expressioni

Tissue specificityi

Present in kidney tubules and in the vasculature of many tissues (at protein level).1 Publication

Developmental stagei

Present in the vasculature and in multiple epithelial populations at E10.5. Present in the cell junctions of the retinal pigmented epithelium at E15.5 (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000045180 Expressed in 229 organ(s), highest expression level in central nervous system
CleanExiMM_SHROOM2
ExpressionAtlasiA2ALU4 baseline and differential
GenevisibleiA2ALU4 MM

Interactioni

Subunit structurei

Interacts with F-actin.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000098701

Structurei

3D structure databases

ProteinModelPortaliA2ALU4
SMRiA2ALU4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 108PDZPROSITE-ProRule annotationAdd BLAST83
Domaini705 – 807ASD1PROSITE-ProRule annotationAdd BLAST103
Domaini1185 – 1476ASD2PROSITE-ProRule annotationAdd BLAST292

Domaini

The ASD1 domain mediates F-actin binding.

Sequence similaritiesi

Belongs to the shroom family.Curated

Phylogenomic databases

eggNOGiENOG410II86 Eukaryota
ENOG41113GY LUCA
GeneTreeiENSGT00530000063061
HOGENOMiHOG000008235
HOVERGENiHBG096290
InParanoidiA2ALU4
KOiK18625

Family and domain databases

InterProiView protein in InterPro
IPR014800 ASD1_dom
IPR014799 ASD2_dom
IPR001478 PDZ
IPR036034 PDZ_sf
IPR027686 Shroom2
IPR027685 Shroom_fam
PANTHERiPTHR15012 PTHR15012, 2 hits
PTHR15012:SF8 PTHR15012:SF8, 2 hits
PfamiView protein in Pfam
PF08688 ASD1, 1 hit
PF08687 ASD2, 1 hit
PF00595 PDZ, 1 hit
SMARTiView protein in SMART
SM00228 PDZ, 1 hit
SUPFAMiSSF50156 SSF50156, 1 hit
PROSITEiView protein in PROSITE
PS51306 ASD1, 1 hit
PS51307 ASD2, 1 hit
PS50106 PDZ, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: A2ALU4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MEGAEPRARP ERLAEAEAPA TDGVRLVEVQ LSGGAPWGFT LKGGREHGEP
60 70 80 90 100
LVITKIEEGS KAAAVDKLLA GDEIVAINDV SLSGFRQEAI CLVKGSHKTL
110 120 130 140 150
KLVVKRKSDP SWRPHSWHAT KYFDVHPEPA ASLFLNTSGS PSWKSQHQAS
160 170 180 190 200
SSSHDLSGSW EHTSLQRTSD HFSSMGSIDS LDHSSQLYPS GHLSSAKSNS
210 220 230 240 250
SIDHLGGHSK RDSAYGSFST CSSTPDHTLP KADASSTENI LYKVGLWEAS
260 270 280 290 300
RPGSSRQSQS TGDPQGLQDR PSCFIPRVPG NSSKSPRPED NVEPKIATHG
310 320 330 340 350
RSNFGPVWYV PDKKKAPSPP PLGLPLRSDS FSVAARGHEK ARGPPFSDLA
360 370 380 390 400
SMQHFITLPH VQPRGDHRME TTDRQWKLTH LSSGKEIGNV GYQSEGHLDC
410 420 430 440 450
RWLCSDDRAG RPSGPPGRLQ FSDVHFLKSY HGSQHQQQCS DESPRAPSSP
460 470 480 490 500
RELLHITPGG GLQEPPEPSQ DDNPTQVRWP GSAHQKLDDR GRSHYFPGSL
510 520 530 540 550
RQPVQGSAQV VIPRGDYWHS DTTPVDLEYP LLRPVGQRTY LQQHEETPAS
560 570 580 590 600
HEKEGYHQLN AGIEGCCSGI QEPPRASRTV RTGLQCPSND FKLVDGESGR
610 620 630 640 650
ISRQRTPMLH SLTQDGTWRP GNSKDCGNDK PPLFDAQVGK PTRRSDRFAT
660 670 680 690 700
TLRNEIQMRR AKLQKSKSTV TLAGDSEAED CAGDWRADVG AVPEGSFPST
710 720 730 740 750
YKEHLKEAQT RVLKATSFQR RDLDPTPADQ YSGPSEHRTF DHSASSSLSS
760 770 780 790 800
FPGEPDSAPR FCETGLAKAP SSGVGVPHVL RIGGRKRFTA EQKLKSYSEP
810 820 830 840 850
EKINEVGLSG DHRPHPTVRT PEDTVGTFAD RWKFFEETSK SLLQKAGHRQ
860 870 880 890 900
VHCGLPREKA ERPQTGHHEC ESTEPWFQKR SLATSCGEIL SDRKVEKASE
910 920 930 940 950
KLNPPRRLGT FAEYQASWKE QKKPLEARSS GRYHSADDIL DAGLDQQQRP
960 970 980 990 1000
QYIHERSRSS PSTDHYSQEV PVEPNRQAED SGDHKEAILC TLQAEEGCSA
1010 1020 1030 1040 1050
PSAQPQDSQH VNEDTTFPQP ETQLSSKCQH LQTSAMETSR SPSPQFAPQK
1060 1070 1080 1090 1100
LTDKPPLLIH EDNSARIERV MDNNTTVKMV PIKIVHSESQ PEKESRQSLA
1110 1120 1130 1140 1150
CPAELPPLPS GLERDQIKTL STSEQCYSRF CVYTRQEVEA PHRARPPEPR
1160 1170 1180 1190 1200
PPSTPAPPVR DSCSSPPSLN YGKAKEKTMD DLKSEELARE IVGKDKSLAD
1210 1220 1230 1240 1250
ILDPSVKIKT TMDLMEGIFP KDEYLLEEAQ QRRKLLPKVP LPRVTEDKKQ
1260 1270 1280 1290 1300
DPGMPGVVSL ATNSTYYSTS APKAELLIKM KDLQEPEEYS AGDLDHDLSV
1310 1320 1330 1340 1350
KKQELIDSIS RKLQVLREAR ESLLEDIQAN NALGDEVEAI VKDVCKPNEF
1360 1370 1380 1390 1400
DKFRMFIGDL DKVVNLLLSL SGRLARVENA LNNLDDNPSP GDRQSLLEKQ
1410 1420 1430 1440 1450
RVLTQQHEDA KELKENLDRR ERIVFDILAT YLSEENLADY EHFVKMKSAL
1460 1470 1480
IIEQRELEDK IHLGEEQLKC LFDSLQPERS K
Length:1,481
Mass (Da):164,703
Last modified:February 20, 2007 - v1
Checksum:i3C5C96100263E393
GO
Isoform 2 (identifier: A2ALU4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1035: Missing.

Show »
Length:446
Mass (Da):50,643
Checksum:iCB52446CCA61912F
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A7TU71A7TU71_MOUSE
Protein Shroom2
Shroom2
1,487Annotation score:

Sequence cautioni

The sequence ABD19518 differs from that shown. Reason: Frameshift at positions 1238, 1244 and 1263.Curated
The sequence BAC26404 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAC27781 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti274F → S in ABD19518 (PubMed:16141072).Curated1
Sequence conflicti458P → S in ABD19518 (PubMed:16141072).Curated1
Sequence conflicti603R → C in ABD19518 (PubMed:16141072).Curated1
Sequence conflicti1012N → K in BAC27781 (PubMed:16141072).Curated1
Sequence conflicti1100A → S in ABD19518 (PubMed:16141072).Curated1
Sequence conflicti1227E → K in ABD19518 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0249641 – 1035Missing in isoform 2. 1 PublicationAdd BLAST1035

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ358971 mRNA Translation: ABD19518.1 Frameshift.
AK029338 mRNA Translation: BAC26404.1 Different initiation.
AK032256 mRNA Translation: BAC27781.1 Different initiation.
AK049850 mRNA Translation: BAC33957.1
AL805960, AL807777 Genomic DNA Translation: CAM17589.1
AL807777, AL805960 Genomic DNA Translation: CAM22989.1
CCDSiCCDS72455.1 [A2ALU4-1]
RefSeqiNP_001277613.1, NM_001290684.1 [A2ALU4-1]
NP_001277614.1, NM_001290685.1
NP_001277615.1, NM_001290686.1 [A2ALU4-2]
NP_001277616.1, NM_001290687.1 [A2ALU4-2]
NP_766029.2, NM_172441.3
XP_011246074.1, XM_011247772.1
XP_011246075.1, XM_011247773.1
XP_011246076.1, XM_011247774.2 [A2ALU4-2]
XP_011246077.1, XM_011247775.2 [A2ALU4-2]
XP_011246078.1, XM_011247776.2
XP_011246079.1, XM_011247777.2 [A2ALU4-2]
UniGeneiMm.40796

Genome annotation databases

EnsembliENSMUST00000062317; ENSMUSP00000057500; ENSMUSG00000045180 [A2ALU4-1]
GeneIDi110380
KEGGimmu:110380
UCSCiuc009uqj.2 mouse [A2ALU4-2]
uc009uqm.2 mouse [A2ALU4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ358971 mRNA Translation: ABD19518.1 Frameshift.
AK029338 mRNA Translation: BAC26404.1 Different initiation.
AK032256 mRNA Translation: BAC27781.1 Different initiation.
AK049850 mRNA Translation: BAC33957.1
AL805960, AL807777 Genomic DNA Translation: CAM17589.1
AL807777, AL805960 Genomic DNA Translation: CAM22989.1
CCDSiCCDS72455.1 [A2ALU4-1]
RefSeqiNP_001277613.1, NM_001290684.1 [A2ALU4-1]
NP_001277614.1, NM_001290685.1
NP_001277615.1, NM_001290686.1 [A2ALU4-2]
NP_001277616.1, NM_001290687.1 [A2ALU4-2]
NP_766029.2, NM_172441.3
XP_011246074.1, XM_011247772.1
XP_011246075.1, XM_011247773.1
XP_011246076.1, XM_011247774.2 [A2ALU4-2]
XP_011246077.1, XM_011247775.2 [A2ALU4-2]
XP_011246078.1, XM_011247776.2
XP_011246079.1, XM_011247777.2 [A2ALU4-2]
UniGeneiMm.40796

3D structure databases

ProteinModelPortaliA2ALU4
SMRiA2ALU4
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000098701

PTM databases

iPTMnetiA2ALU4
PhosphoSitePlusiA2ALU4

Proteomic databases

MaxQBiA2ALU4
PaxDbiA2ALU4
PeptideAtlasiA2ALU4
PRIDEiA2ALU4

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000062317; ENSMUSP00000057500; ENSMUSG00000045180 [A2ALU4-1]
GeneIDi110380
KEGGimmu:110380
UCSCiuc009uqj.2 mouse [A2ALU4-2]
uc009uqm.2 mouse [A2ALU4-1]

Organism-specific databases

CTDi357
MGIiMGI:107194 Shroom2

Phylogenomic databases

eggNOGiENOG410II86 Eukaryota
ENOG41113GY LUCA
GeneTreeiENSGT00530000063061
HOGENOMiHOG000008235
HOVERGENiHBG096290
InParanoidiA2ALU4
KOiK18625

Miscellaneous databases

PROiPR:A2ALU4
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000045180 Expressed in 229 organ(s), highest expression level in central nervous system
CleanExiMM_SHROOM2
ExpressionAtlasiA2ALU4 baseline and differential
GenevisibleiA2ALU4 MM

Family and domain databases

InterProiView protein in InterPro
IPR014800 ASD1_dom
IPR014799 ASD2_dom
IPR001478 PDZ
IPR036034 PDZ_sf
IPR027686 Shroom2
IPR027685 Shroom_fam
PANTHERiPTHR15012 PTHR15012, 2 hits
PTHR15012:SF8 PTHR15012:SF8, 2 hits
PfamiView protein in Pfam
PF08688 ASD1, 1 hit
PF08687 ASD2, 1 hit
PF00595 PDZ, 1 hit
SMARTiView protein in SMART
SM00228 PDZ, 1 hit
SUPFAMiSSF50156 SSF50156, 1 hit
PROSITEiView protein in PROSITE
PS51306 ASD1, 1 hit
PS51307 ASD2, 1 hit
PS50106 PDZ, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSHRM2_MOUSE
AccessioniPrimary (citable) accession number: A2ALU4
Secondary accession number(s): Q2EY23
, Q8C7N1, Q8CCR2, Q8CDZ0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: February 20, 2007
Last modified: November 7, 2018
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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