Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

UniProtKB - A2AGT5 (CKAP5_MOUSE)

Protein

Cytoskeleton-associated protein 5

Gene

Ckap5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds to the plus end of microtubules and regulates microtubule dynamics and microtubule organization. Acts as processive microtubule polymerase. Promotes cytoplasmic microtubule nucleation and elongation. Plays a major role in organizing spindle poles. In spindle formation protects kinetochore microtubules from depolymerization by KIF2C and has an essential role in centrosomal microtubule assembly independently of KIF2C activity. Contributes to centrosome integrity. Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge. The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension. Enhances the strength of NDC80 complex-mediated kinetochore-tip microtubule attachments.By similarity

GO - Molecular functioni

  • microtubule binding Source: MGI
  • microtubule plus-end binding Source: GO_Central
  • ribonucleoprotein complex binding Source: MGI
View the complete GO annotation on QuickGO ...

GO - Biological processi

View the complete GO annotation on QuickGO ...

Keywordsi

Biological processCell cycle, Cell division, Mitosis

Names & Taxonomyi

Protein namesi
Recommended name:
Cytoskeleton-associated protein 5
Gene namesi
Name:Ckap5Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1923036 Ckap5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003640051 – 2032Cytoskeleton-associated protein 5Add BLAST2032

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei48N6-acetyllysineBy similarity1
Modified residuei816PhosphoserineBy similarity1
Modified residuei1469PhosphoserineBy similarity1
Modified residuei1861PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiA2AGT5
PaxDbiA2AGT5
PeptideAtlasiA2AGT5
PRIDEiA2AGT5

PTM databases

iPTMnetiA2AGT5
PhosphoSitePlusiA2AGT5
SwissPalmiA2AGT5

Interactioni

Subunit structurei

Interacts with TACC1. Interacts with HNRNPA2B1. Interacts with TACC3 independently of clathrin. Interacts with TACC3 and clathrin forming the TACC3/ch-TOG/clathrin complex located at spindle inter-microtubules bridges. Interacts with NDC80; indicative for an association with the NDC80 complex. Interacts with SLAIN2 (By similarity). Interacts with SLAIN1 (PubMed:21646404).By similarity1 Publication

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridi217741, 34 interactors
IntActiA2AGT5, 32 interactors
STRINGi10090.ENSMUSP00000097303

Structurei

3D structure databases

ProteinModelPortaliA2AGT5
SMRiA2AGT5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati159 – 197HEAT 1Sequence analysisAdd BLAST39
Repeati356 – 394HEAT 2Sequence analysisAdd BLAST39
Repeati434 – 472HEAT 3Sequence analysisAdd BLAST39
Repeati750 – 788HEAT 4Sequence analysisAdd BLAST39
Repeati855 – 893HEAT 5Sequence analysisAdd BLAST39
Repeati936 – 974HEAT 6Sequence analysisAdd BLAST39
Repeati1013 – 1051HEAT 7Sequence analysisAdd BLAST39
Repeati1284 – 1322HEAT 8Sequence analysisAdd BLAST39
Repeati1324 – 1357HEAT 9Sequence analysisAdd BLAST34
Repeati1361 – 1399HEAT 10Sequence analysisAdd BLAST39

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 223TOG 1By similarityAdd BLAST223
Regioni268 – 502TOG 2By similarityAdd BLAST235
Regioni588 – 817TOG 3By similarityAdd BLAST230
Regioni853 – 1081TOG 4By similarityAdd BLAST229
Regioni1193 – 1428TOG 5By similarityAdd BLAST236
Regioni1932 – 1957Interaction with TACC3By similarityAdd BLAST26

Domaini

The TOG (tumor overexpressed gene) domains are arranged in a N-terminal pentameric array with each domain composed of six (for the most part non-canonical) HEAT repeats forming a oblong paddle-like structure. Intra-HEAT loops are positioned along a face of the TOG domain and bind to a single alpha/beta-tubulin heterodimer. The TOG domains in the array seem to be structurally and functionally polarized. Differential functions may range from microtubule (MT) lattice binding and/or free tubulin heterodimer binding to potentiating stable incorporation of tubulin into the MT lattice.By similarity

Sequence similaritiesi

Belongs to the TOG/XMAP215 family.Sequence analysis

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1820 Eukaryota
ENOG410XPTW LUCA
HOVERGENiHBG050955
InParanoidiA2AGT5
KOiK16803
PhylomeDBiA2AGT5

Family and domain databases

Gene3Di1.25.10.10, 5 hits
InterProiView protein in InterPro
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR024395 CLASP_N_dom
IPR021133 HEAT_type_2
IPR034085 TOG
PfamiView protein in Pfam
PF12348 CLASP_N, 3 hits
SMARTiView protein in SMART
SM01349 TOG, 5 hits
SUPFAMiSSF48371 SSF48371, 2 hits
PROSITEiView protein in PROSITE
PS50077 HEAT_REPEAT, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 11 Publication (identifier: A2AGT5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGDDSEWLKL PVDQKCEHKL WKARLSGYEE ALKIFQKIKD EKSPEWSKYL 
        60         70         80         90        100
GLIKKFVTDS NAVVQLKGLE AALVYVENAH VAGKTTGEVV SGVVSKVFNQ 
       110        120        130        140        150
PKAKAKELGI EICLMYVEIE KGESVQEELL KGLDNKNPKI IVACIETLRK 
       160        170        180        190        200
ALSEFGSKII SLKPIIKVLP KLFESRDKAV RDEAKLFAIE IYRWNRDAVK 
       210        220        230        240        250
HTLQNINSVQ LKELEEEWVK LPTGAPKPSR FLRSQQELEA KLEQQQSAGG 
       260        270        280        290        300
DAEGGGDDGD EVPQVDAYEL LDAVEILSKL PKDFYDKIEA KKWQERKEAL 
       310        320        330        340        350
EAVEVLVKNP KLEAGDYADL VKALKKVVGK DTNVMLVALA AKCLTGLAVG 
       360        370        380        390        400
LRKKFGQYAG HVVPTILEKF KEKKPQVVQA LQEAIDAIFL TTTLQNISED 
       410        420        430        440        450
VLAVMDNKNP TIKQQTSLFI ARSFRHCTSS TLPKSLLKPF CAALLKHIND 
       460        470        480        490        500
SAPEVRDAAF EALGTALKVV GEKSVNPFLA DVDKLKLDRI KECSEKVELV 
       510        520        530        540        550
HGKKSGLATE KKESKPLPGR AAASGAAGDK DTKDVSGPKP GPLKKTPTAK 
       560        570        580        590        600
AGGPSKKGKT TAPGGSASAG TKNKKGLETK EIVEPELSIE VCEEKASAVL 
       610        620        630        640        650
PPTCIQLLDS SNWKERLACM EEFQKAVELM ERTEMPCQAL VKMLAKKPGW 
       660        670        680        690        700
KETNFQVMQM KLHIVALIAQ KGNFSKTSAQ IVLDGLVDKI GDVKCGNNAK 
       710        720        730        740        750
EAMTAIAEAC MLPWTAEQVM SMAFSQKNPK NQSETLNWLS NAIKEFGFSE 
       760        770        780        790        800
LNVKAFISNV KTALAATNPA VRTSAITLLG VMYLYVGPSL RMIFEDEKPA 
       810        820        830        840        850
LLSQIDAEFQ KMQGQSPPAP TRGIAKHSTS ATDEGEDGEE PGEGGNDVVD 
       860        870        880        890        900
LLPRIEISDK ITSELVSKIG DKNWKIRKEG LDEVAGIINE AKFIQPNIGE 
       910        920        930        940        950
LPTALKGRLN DSNKILVQQT LNILQQLAVA MGANIRQHVK NLGIPVITVL 
       960        970        980        990       1000
GDSKNNVRAA ALATVNAWAE QTGMKEWLEG EDLSEELKKE NPFLRQELLG 
      1010       1020       1030       1040       1050
WLAEKLPTLR STPTDLILCV PHLYSCLKDR NGDVRKKAQD ALPFFMMHLG 
      1060       1070       1080       1090       1100
YEKMAKATGK LKPTSKDQVL AMLEKAKANM PSKPAAPAKA MSKPMGGSAP 
      1110       1120       1130       1140       1150
AKTQPIPAPV EDSVSSTIEA KPDLKKAKAP GVSSKAKSVQ GKKVPSKTTL 
      1160       1170       1180       1190       1200
KEDDDKSGPI FIVVPNGKEQ RMRDEKGLKV LKWNFTTPRD EYIEQLKTQM 
      1210       1220       1230       1240       1250
STCVAKWLQD EMFHSDFQHH NKALAVMVDH LESEKDGVIS CLDLILKWLT 
      1260       1270       1280       1290       1300
LRFFDTNTSV LMKALEYLKL LFTLLSEEEY HLTENEASSF IPYLILKVGE 
      1310       1320       1330       1340       1350
PKDVIRKDVR AILNRMCLVY PASKMFPFIM EGTKSKNSKQ RAECLEELGC 
      1360       1370       1380       1390       1400
LIESYGMNVC QPTPGKALKE IAIHIGDRDN AVRNAALNTI VTVYNVHGDQ 
      1410       1420       1430       1440       1450
VFKLIGNLSE KDMSMLEERI KRSAKRPSAA PVKQAEEKPQ RTQNINSNAN 
      1460       1470       1480       1490       1500
MLRKGPAEDM SSKLNQARSL SGHPEAAQMV RREFQLDLDE IENDNGTVRC 
      1510       1520       1530       1540       1550
EMPELVQHKL DDIFEPVLIP EPKIRAVSPH FDDMHSNTAS TINFIISQVA 
      1560       1570       1580       1590       1600
SGDINTSIQA LTQIDEVLRQ EDKAEAMSGH IDQFLIATFM QLRLIYSTHM 
      1610       1620       1630       1640       1650
ADEKLDKDEI IKLYSCIIGN MISLFQIESL AREASTGVLK DLMHGLITLM 
      1660       1670       1680       1690       1700
LDSRIEDLEE GQQVIRSVNL LVVKVLEKSD QTNILSALLV LLQDSLLATA 
      1710       1720       1730       1740       1750
SSPKFSELVM KCLWRMVRLL PDTINSINLD RILLDIHIFM KVFPKEKLKQ 
      1760       1770       1780       1790       1800
CKSEFPIRTL KTLLHTLCKL KGPKILDHLT MIDNKNESEL EAHLCRMMKH 
      1810       1820       1830       1840       1850
SMDQTGSKSD KETEKGASRI DEKSSKAKVN DFLAEIFKKI GSKENTKEGL 
      1860       1870       1880       1890       1900
AELYEYKKKY SDTDIEPFLK NSSQFFQSYV ERGLRVIEME RESKGRIPTS 
      1910       1920       1930       1940       1950
TGISPQMEVT CVPTPTSTVS SLGNTNGEEV GPSVYLERLK ILRQRCGLDN 
      1960       1970       1980       1990       2000
TKQDDRPPLT SLLSKPAIPP VASSTDMLHS KLSQLRESRE QHQHSDLDSN 
      2010       2020       2030 
QTHSAGTMTS SSSTTNIDDL KKRLERIKSS RK
Length:2,032
Mass (Da):225,635
Last modified:February 20, 2007 - v1
Checksum:i28FD8628C6F678CB
GO
Isoform 2 (identifier: A2AGT5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1564-1623: Missing.

Note: No experimental confirmation available. Gene prediction based on similarity to human ortholog.
Show »
Length:1,972
Mass (Da):218,707
Checksum:iF33AAA7B32F32540
GO
Isoform 31 Publication (identifier: A2AGT5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1903-1923: Missing.

Show »
Length:2,011
Mass (Da):223,518
Checksum:i0F8E07DCC94668C5
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
K3W4R5K3W4R5_MOUSE
Cytoskeleton-associated protein 5
Ckap5
2,032Annotation score:
A0A0R4J0K2A0A0R4J0K2_MOUSE
Cytoskeleton-associated protein 5
Ckap5
2,011Annotation score:
Z4YL78Z4YL78_MOUSE
Cytoskeleton-associated protein 5
Ckap5
1,972Annotation score:

Sequence cautioni

The sequence AAH17140 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti5S → C in BAE38422 (PubMed:16141072).Curated1
Sequence conflicti19K → N in BAB29779 (PubMed:16141072).Curated1
Sequence conflicti111E → K in BAB29779 (PubMed:16141072).Curated1
Sequence conflicti174E → G in BAE38422 (PubMed:16141072).Curated1
Sequence conflicti227K → R in BAB29779 (PubMed:16141072).Curated1
Sequence conflicti522A → T in BAB29779 (PubMed:16141072).Curated1
Sequence conflicti581E → Q in BAB29779 (PubMed:16141072).Curated1
Sequence conflicti675S → F in BAB29779 (PubMed:16141072).Curated1
Sequence conflicti709A → G in BAB29779 (PubMed:16141072).Curated1
Sequence conflicti836E → V in BAE29125 (PubMed:16141072).Curated1
Sequence conflicti981E → A in AAH89032 (PubMed:15489334).Curated1
Sequence conflicti1028K → E in AAH89032 (PubMed:15489334).Curated1
Sequence conflicti1028K → E in AAH96422 (PubMed:15489334).Curated1
Sequence conflicti1028K → E in AAI38335 (PubMed:15489334).Curated1
Sequence conflicti1717V → F in AAH96422 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0530031564 – 1623Missing in isoform 2. CuratedAdd BLAST60
Alternative sequenceiVSP_0530041903 – 1923Missing in isoform 3. 1 PublicationAdd BLAST21

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL691489 Genomic DNA No translation available.
BC017140 mRNA Translation: AAH17140.1 Sequence problems.
BC089032 mRNA Translation: AAH89032.1
BC096422 mRNA Translation: AAH96422.1
BC138334 mRNA Translation: AAI38335.1
AK015282 mRNA Translation: BAB29779.1
AK149854 mRNA Translation: BAE29125.1
AK165862 mRNA Translation: BAE38422.1
CCDSiCCDS16433.1 [A2AGT5-3]
CCDS50640.1 [A2AGT5-1]
RefSeqiNP_083713.2, NM_029437.2
UniGeneiMm.168478

Genome annotation databases

GeneIDi75786
KEGGimmu:75786
UCSCiuc008kwd.1 mouse [A2AGT5-3]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL691489 Genomic DNA No translation available.
BC017140 mRNA Translation: AAH17140.1 Sequence problems.
BC089032 mRNA Translation: AAH89032.1
BC096422 mRNA Translation: AAH96422.1
BC138334 mRNA Translation: AAI38335.1
AK015282 mRNA Translation: BAB29779.1
AK149854 mRNA Translation: BAE29125.1
AK165862 mRNA Translation: BAE38422.1
CCDSiCCDS16433.1 [A2AGT5-3]
CCDS50640.1 [A2AGT5-1]
RefSeqiNP_083713.2, NM_029437.2
UniGeneiMm.168478

3D structure databases

ProteinModelPortaliA2AGT5
SMRiA2AGT5
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi217741, 34 interactors
IntActiA2AGT5, 32 interactors
STRINGi10090.ENSMUSP00000097303

PTM databases

iPTMnetiA2AGT5
PhosphoSitePlusiA2AGT5
SwissPalmiA2AGT5

Proteomic databases

MaxQBiA2AGT5
PaxDbiA2AGT5
PeptideAtlasiA2AGT5
PRIDEiA2AGT5

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi75786
KEGGimmu:75786
UCSCiuc008kwd.1 mouse [A2AGT5-3]

Organism-specific databases

CTDi9793
MGIiMGI:1923036 Ckap5

Phylogenomic databases

eggNOGiKOG1820 Eukaryota
ENOG410XPTW LUCA
HOVERGENiHBG050955
InParanoidiA2AGT5
KOiK16803
PhylomeDBiA2AGT5

Miscellaneous databases

PROiPR:A2AGT5
SOURCEiSearch...

Family and domain databases

Gene3Di1.25.10.10, 5 hits
InterProiView protein in InterPro
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR024395 CLASP_N_dom
IPR021133 HEAT_type_2
IPR034085 TOG
PfamiView protein in Pfam
PF12348 CLASP_N, 3 hits
SMARTiView protein in SMART
SM01349 TOG, 5 hits
SUPFAMiSSF48371 SSF48371, 2 hits
PROSITEiView protein in PROSITE
PS50077 HEAT_REPEAT, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCKAP5_MOUSE
AccessioniPrimary (citable) accession number: A2AGT5
Secondary accession number(s): A0PJ77
, A2AGT6, B2RRC4, Q0VGR0, Q3TML9, Q3UDY6, Q4VAE7, Q9CUN0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: February 20, 2007
Last modified: November 7, 2018
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again