UniProtKB - A2AGT5 (CKAP5_MOUSE)
Cytoskeleton-associated protein 5
Ckap5
Functioni
GO - Molecular functioni
- microtubule binding Source: MGI
- microtubule plus end polymerase Source: GO_Central
- ribonucleoprotein complex binding Source: MGI
GO - Biological processi
- cell division Source: UniProtKB-KW
- central nervous system myelin formation Source: MGI
- centrosome cycle Source: MGI
- centrosome duplication Source: GO_Central
- cytoplasmic translation Source: MGI
- dendritic spine maintenance Source: MGI
- establishment or maintenance of microtubule cytoskeleton polarity Source: MGI
- excitatory postsynaptic potential Source: MGI
- habituation Source: MGI
- locomotory behavior Source: MGI
- long-term synaptic potentiation Source: MGI
- microtubule polymerization Source: GO_Central
- mitotic spindle organization Source: GO_Central
- mRNA localization resulting in posttranscriptional regulation of gene expression Source: MGI
- positive regulation of endocytosis Source: MGI
- positive regulation of microtubule nucleation Source: MGI
- ribonucleoprotein complex assembly Source: MGI
- RNA transport Source: UniProtKB
- spindle organization Source: MGI
- visual learning Source: MGI
Keywordsi
Biological process | Cell cycle, Cell division, Mitosis |
Enzyme and pathway databases
Reactomei | R-MMU-141444, Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal R-MMU-2467813, Separation of Sister Chromatids R-MMU-2500257, Resolution of Sister Chromatid Cohesion R-MMU-2565942, Regulation of PLK1 Activity at G2/M Transition R-MMU-380259, Loss of Nlp from mitotic centrosomes R-MMU-380270, Recruitment of mitotic centrosome proteins and complexes R-MMU-380284, Loss of proteins required for interphase microtubule organization from the centrosome R-MMU-380320, Recruitment of NuMA to mitotic centrosomes R-MMU-5620912, Anchoring of the basal body to the plasma membrane R-MMU-5663220, RHO GTPases Activate Formins R-MMU-68877, Mitotic Prometaphase R-MMU-8854518, AURKA Activation by TPX2 R-MMU-9648025, EML4 and NUDC in mitotic spindle formation |
Names & Taxonomyi
Protein namesi | Recommended name: Cytoskeleton-associated protein 5 |
Gene namesi | Name:Ckap5Imported |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1923036, Ckap5 |
Subcellular locationi
Cytoskeleton
- centrosome By similarity
- spindle pole By similarity
- spindle By similarity
Other locations
- kinetochore By similarity
Note: Detected on centrosomes and kinetochores during interphase and mitosis independently from TACC3 and clathrin. Located to spindle poles and microtubules during mitosis. In complex with TACC3 localized to microtubule plus-ends in mitosis and interphase. In complex with TACC3 and clathrin localized to inter-microtubule bridges in mitotic spindles. Accumulation sites at microtubule plus ends protruded approximately 100 nm from MAPRE1/EB1 sites in interphase cells.By similarity
Cytoskeleton
- centrosome Source: MGI
- spindle pole Source: MGI
Nucleus
- nucleolus Source: MGI
Plasma Membrane
- plasma membrane Source: MGI
Other locations
- condensed chromosome kinetochore Source: UniProtKB-SubCell
- cytoplasm Source: MGI
- dendrite Source: MGI
- kinetochore Source: MGI
- neuronal ribonucleoprotein granule Source: MGI
- perikaryon Source: MGI
- postsynapse Source: GOC
- protein-containing complex Source: MGI
Keywords - Cellular componenti
Centromere, Chromosome, Cytoplasm, Cytoskeleton, KinetochorePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000364005 | 1 – 2032 | Cytoskeleton-associated protein 5Add BLAST | 2032 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 48 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 816 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1469 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1861 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
jPOSTi | A2AGT5 |
MaxQBi | A2AGT5 |
PaxDbi | A2AGT5 |
PeptideAtlasi | A2AGT5 |
PRIDEi | A2AGT5 |
ProteomicsDBi | 283624 [A2AGT5-1] 283625 [A2AGT5-2] 283626 [A2AGT5-3] |
PTM databases
iPTMneti | A2AGT5 |
PhosphoSitePlusi | A2AGT5 |
SwissPalmi | A2AGT5 |
Interactioni
Subunit structurei
Interacts with TACC1.
Interacts with HNRNPA2B1.
Interacts with TACC3 independently of clathrin.
Interacts with TACC3 and clathrin forming the TACC3/ch-TOG/clathrin complex located at spindle inter-microtubules bridges.
Interacts with NDC80; indicative for an association with the NDC80 complex.
Interacts with SLAIN2 (By similarity).
Interacts with SLAIN1 (PubMed:21646404).
By similarity1 PublicationGO - Molecular functioni
- microtubule binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 217741, 56 interactors |
IntActi | A2AGT5, 32 interactors |
STRINGi | 10090.ENSMUSP00000106970 |
Miscellaneous databases
RNActi | A2AGT5, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 159 – 197 | HEAT 1Sequence analysisAdd BLAST | 39 | |
Repeati | 356 – 394 | HEAT 2Sequence analysisAdd BLAST | 39 | |
Repeati | 434 – 472 | HEAT 3Sequence analysisAdd BLAST | 39 | |
Repeati | 750 – 788 | HEAT 4Sequence analysisAdd BLAST | 39 | |
Repeati | 855 – 893 | HEAT 5Sequence analysisAdd BLAST | 39 | |
Repeati | 936 – 974 | HEAT 6Sequence analysisAdd BLAST | 39 | |
Repeati | 1013 – 1051 | HEAT 7Sequence analysisAdd BLAST | 39 | |
Repeati | 1284 – 1322 | HEAT 8Sequence analysisAdd BLAST | 39 | |
Repeati | 1324 – 1357 | HEAT 9Sequence analysisAdd BLAST | 34 | |
Repeati | 1361 – 1399 | HEAT 10Sequence analysisAdd BLAST | 39 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 223 | TOG 1By similarityAdd BLAST | 223 | |
Regioni | 268 – 502 | TOG 2By similarityAdd BLAST | 235 | |
Regioni | 588 – 817 | TOG 3By similarityAdd BLAST | 230 | |
Regioni | 853 – 1081 | TOG 4By similarityAdd BLAST | 229 | |
Regioni | 1193 – 1428 | TOG 5By similarityAdd BLAST | 236 | |
Regioni | 1932 – 1957 | Interaction with TACC3By similarityAdd BLAST | 26 |
Domaini
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG1820, Eukaryota |
InParanoidi | A2AGT5 |
OrthoDBi | 33681at2759 |
PhylomeDBi | A2AGT5 |
Family and domain databases
Gene3Di | 1.25.10.10, 5 hits |
InterProi | View protein in InterPro IPR011989, ARM-like IPR016024, ARM-type_fold IPR024395, CLASP_N_dom IPR021133, HEAT_type_2 IPR034085, TOG |
Pfami | View protein in Pfam PF12348, CLASP_N, 3 hits |
SMARTi | View protein in SMART SM01349, TOG, 5 hits |
SUPFAMi | SSF48371, SSF48371, 2 hits |
PROSITEi | View protein in PROSITE PS50077, HEAT_REPEAT, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGDDSEWLKL PVDQKCEHKL WKARLSGYEE ALKIFQKIKD EKSPEWSKYL
60 70 80 90 100
GLIKKFVTDS NAVVQLKGLE AALVYVENAH VAGKTTGEVV SGVVSKVFNQ
110 120 130 140 150
PKAKAKELGI EICLMYVEIE KGESVQEELL KGLDNKNPKI IVACIETLRK
160 170 180 190 200
ALSEFGSKII SLKPIIKVLP KLFESRDKAV RDEAKLFAIE IYRWNRDAVK
210 220 230 240 250
HTLQNINSVQ LKELEEEWVK LPTGAPKPSR FLRSQQELEA KLEQQQSAGG
260 270 280 290 300
DAEGGGDDGD EVPQVDAYEL LDAVEILSKL PKDFYDKIEA KKWQERKEAL
310 320 330 340 350
EAVEVLVKNP KLEAGDYADL VKALKKVVGK DTNVMLVALA AKCLTGLAVG
360 370 380 390 400
LRKKFGQYAG HVVPTILEKF KEKKPQVVQA LQEAIDAIFL TTTLQNISED
410 420 430 440 450
VLAVMDNKNP TIKQQTSLFI ARSFRHCTSS TLPKSLLKPF CAALLKHIND
460 470 480 490 500
SAPEVRDAAF EALGTALKVV GEKSVNPFLA DVDKLKLDRI KECSEKVELV
510 520 530 540 550
HGKKSGLATE KKESKPLPGR AAASGAAGDK DTKDVSGPKP GPLKKTPTAK
560 570 580 590 600
AGGPSKKGKT TAPGGSASAG TKNKKGLETK EIVEPELSIE VCEEKASAVL
610 620 630 640 650
PPTCIQLLDS SNWKERLACM EEFQKAVELM ERTEMPCQAL VKMLAKKPGW
660 670 680 690 700
KETNFQVMQM KLHIVALIAQ KGNFSKTSAQ IVLDGLVDKI GDVKCGNNAK
710 720 730 740 750
EAMTAIAEAC MLPWTAEQVM SMAFSQKNPK NQSETLNWLS NAIKEFGFSE
760 770 780 790 800
LNVKAFISNV KTALAATNPA VRTSAITLLG VMYLYVGPSL RMIFEDEKPA
810 820 830 840 850
LLSQIDAEFQ KMQGQSPPAP TRGIAKHSTS ATDEGEDGEE PGEGGNDVVD
860 870 880 890 900
LLPRIEISDK ITSELVSKIG DKNWKIRKEG LDEVAGIINE AKFIQPNIGE
910 920 930 940 950
LPTALKGRLN DSNKILVQQT LNILQQLAVA MGANIRQHVK NLGIPVITVL
960 970 980 990 1000
GDSKNNVRAA ALATVNAWAE QTGMKEWLEG EDLSEELKKE NPFLRQELLG
1010 1020 1030 1040 1050
WLAEKLPTLR STPTDLILCV PHLYSCLKDR NGDVRKKAQD ALPFFMMHLG
1060 1070 1080 1090 1100
YEKMAKATGK LKPTSKDQVL AMLEKAKANM PSKPAAPAKA MSKPMGGSAP
1110 1120 1130 1140 1150
AKTQPIPAPV EDSVSSTIEA KPDLKKAKAP GVSSKAKSVQ GKKVPSKTTL
1160 1170 1180 1190 1200
KEDDDKSGPI FIVVPNGKEQ RMRDEKGLKV LKWNFTTPRD EYIEQLKTQM
1210 1220 1230 1240 1250
STCVAKWLQD EMFHSDFQHH NKALAVMVDH LESEKDGVIS CLDLILKWLT
1260 1270 1280 1290 1300
LRFFDTNTSV LMKALEYLKL LFTLLSEEEY HLTENEASSF IPYLILKVGE
1310 1320 1330 1340 1350
PKDVIRKDVR AILNRMCLVY PASKMFPFIM EGTKSKNSKQ RAECLEELGC
1360 1370 1380 1390 1400
LIESYGMNVC QPTPGKALKE IAIHIGDRDN AVRNAALNTI VTVYNVHGDQ
1410 1420 1430 1440 1450
VFKLIGNLSE KDMSMLEERI KRSAKRPSAA PVKQAEEKPQ RTQNINSNAN
1460 1470 1480 1490 1500
MLRKGPAEDM SSKLNQARSL SGHPEAAQMV RREFQLDLDE IENDNGTVRC
1510 1520 1530 1540 1550
EMPELVQHKL DDIFEPVLIP EPKIRAVSPH FDDMHSNTAS TINFIISQVA
1560 1570 1580 1590 1600
SGDINTSIQA LTQIDEVLRQ EDKAEAMSGH IDQFLIATFM QLRLIYSTHM
1610 1620 1630 1640 1650
ADEKLDKDEI IKLYSCIIGN MISLFQIESL AREASTGVLK DLMHGLITLM
1660 1670 1680 1690 1700
LDSRIEDLEE GQQVIRSVNL LVVKVLEKSD QTNILSALLV LLQDSLLATA
1710 1720 1730 1740 1750
SSPKFSELVM KCLWRMVRLL PDTINSINLD RILLDIHIFM KVFPKEKLKQ
1760 1770 1780 1790 1800
CKSEFPIRTL KTLLHTLCKL KGPKILDHLT MIDNKNESEL EAHLCRMMKH
1810 1820 1830 1840 1850
SMDQTGSKSD KETEKGASRI DEKSSKAKVN DFLAEIFKKI GSKENTKEGL
1860 1870 1880 1890 1900
AELYEYKKKY SDTDIEPFLK NSSQFFQSYV ERGLRVIEME RESKGRIPTS
1910 1920 1930 1940 1950
TGISPQMEVT CVPTPTSTVS SLGNTNGEEV GPSVYLERLK ILRQRCGLDN
1960 1970 1980 1990 2000
TKQDDRPPLT SLLSKPAIPP VASSTDMLHS KLSQLRESRE QHQHSDLDSN
2010 2020 2030
QTHSAGTMTS SSSTTNIDDL KKRLERIKSS RK
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketK3W4R5 | K3W4R5_MOUSE | Cytoskeleton-associated protein 5 | Ckap5 | 2,032 | Annotation score: | ||
A0A0R4J0K2 | A0A0R4J0K2_MOUSE | Cytoskeleton-associated protein 5 | Ckap5 | 2,011 | Annotation score: | ||
Z4YL78 | Z4YL78_MOUSE | Cytoskeleton-associated protein 5 | Ckap5 | 1,972 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 5 | S → C in BAE38422 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 19 | K → N in BAB29779 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 111 | E → K in BAB29779 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 174 | E → G in BAE38422 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 227 | K → R in BAB29779 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 522 | A → T in BAB29779 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 581 | E → Q in BAB29779 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 675 | S → F in BAB29779 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 709 | A → G in BAB29779 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 836 | E → V in BAE29125 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 981 | E → A in AAH89032 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 1028 | K → E in AAH89032 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 1028 | K → E in AAH96422 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 1028 | K → E in AAI38335 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 1717 | V → F in AAH96422 (PubMed:15489334).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_053003 | 1564 – 1623 | Missing in isoform 2. CuratedAdd BLAST | 60 | |
Alternative sequenceiVSP_053004 | 1903 – 1923 | Missing in isoform 3. 1 PublicationAdd BLAST | 21 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL691489 Genomic DNA No translation available. BC017140 mRNA Translation: AAH17140.1 Sequence problems. BC089032 mRNA Translation: AAH89032.1 BC096422 mRNA Translation: AAH96422.1 BC138334 mRNA Translation: AAI38335.1 AK015282 mRNA Translation: BAB29779.1 AK149854 mRNA Translation: BAE29125.1 AK165862 mRNA Translation: BAE38422.1 |
CCDSi | CCDS16433.1 [A2AGT5-3] CCDS50640.1 [A2AGT5-1] |
RefSeqi | NP_083713.2, NM_029437.2 |
Genome annotation databases
GeneIDi | 75786 |
KEGGi | mmu:75786 |
UCSCi | uc008kwd.1, mouse [A2AGT5-3] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL691489 Genomic DNA No translation available. BC017140 mRNA Translation: AAH17140.1 Sequence problems. BC089032 mRNA Translation: AAH89032.1 BC096422 mRNA Translation: AAH96422.1 BC138334 mRNA Translation: AAI38335.1 AK015282 mRNA Translation: BAB29779.1 AK149854 mRNA Translation: BAE29125.1 AK165862 mRNA Translation: BAE38422.1 |
CCDSi | CCDS16433.1 [A2AGT5-3] CCDS50640.1 [A2AGT5-1] |
RefSeqi | NP_083713.2, NM_029437.2 |
3D structure databases
SMRi | A2AGT5 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 217741, 56 interactors |
IntActi | A2AGT5, 32 interactors |
STRINGi | 10090.ENSMUSP00000106970 |
PTM databases
iPTMneti | A2AGT5 |
PhosphoSitePlusi | A2AGT5 |
SwissPalmi | A2AGT5 |
Proteomic databases
jPOSTi | A2AGT5 |
MaxQBi | A2AGT5 |
PaxDbi | A2AGT5 |
PeptideAtlasi | A2AGT5 |
PRIDEi | A2AGT5 |
ProteomicsDBi | 283624 [A2AGT5-1] 283625 [A2AGT5-2] 283626 [A2AGT5-3] |
Genome annotation databases
GeneIDi | 75786 |
KEGGi | mmu:75786 |
UCSCi | uc008kwd.1, mouse [A2AGT5-3] |
Organism-specific databases
CTDi | 9793 |
MGIi | MGI:1923036, Ckap5 |
Phylogenomic databases
eggNOGi | KOG1820, Eukaryota |
InParanoidi | A2AGT5 |
OrthoDBi | 33681at2759 |
PhylomeDBi | A2AGT5 |
Enzyme and pathway databases
Reactomei | R-MMU-141444, Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal R-MMU-2467813, Separation of Sister Chromatids R-MMU-2500257, Resolution of Sister Chromatid Cohesion R-MMU-2565942, Regulation of PLK1 Activity at G2/M Transition R-MMU-380259, Loss of Nlp from mitotic centrosomes R-MMU-380270, Recruitment of mitotic centrosome proteins and complexes R-MMU-380284, Loss of proteins required for interphase microtubule organization from the centrosome R-MMU-380320, Recruitment of NuMA to mitotic centrosomes R-MMU-5620912, Anchoring of the basal body to the plasma membrane R-MMU-5663220, RHO GTPases Activate Formins R-MMU-68877, Mitotic Prometaphase R-MMU-8854518, AURKA Activation by TPX2 R-MMU-9648025, EML4 and NUDC in mitotic spindle formation |
Miscellaneous databases
BioGRID-ORCSi | 75786, 17 hits in 52 CRISPR screens |
ChiTaRSi | Ckap5, mouse |
PROi | PR:A2AGT5 |
RNActi | A2AGT5, protein |
SOURCEi | Search... |
Family and domain databases
Gene3Di | 1.25.10.10, 5 hits |
InterProi | View protein in InterPro IPR011989, ARM-like IPR016024, ARM-type_fold IPR024395, CLASP_N_dom IPR021133, HEAT_type_2 IPR034085, TOG |
Pfami | View protein in Pfam PF12348, CLASP_N, 3 hits |
SMARTi | View protein in SMART SM01349, TOG, 5 hits |
SUPFAMi | SSF48371, SSF48371, 2 hits |
PROSITEi | View protein in PROSITE PS50077, HEAT_REPEAT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CKAP5_MOUSE | |
Accessioni | A2AGT5Primary (citable) accession number: A2AGT5 Secondary accession number(s): A0PJ77 Q9CUN0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 10, 2009 |
Last sequence update: | February 20, 2007 | |
Last modified: | April 7, 2021 | |
This is version 116 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families