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Protein

Mediator of RNA polymerase II transcription subunit 12

Gene

Med12

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, Repressor
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-212436 Generic Transcription Pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 12
Alternative name(s):
Mediator complex subunit 12
OPA-containing protein
Thyroid hormone receptor-associated protein complex 230 kDa component
Short name:
Trap230
Trinucleotide repeat-containing gene 11 protein
Gene namesi
Name:Med12
Synonyms:Kiaa0192, Mopa, Tnrc11, Trap230
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:1926212 Med12

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003129571 – 2190Mediator of RNA polymerase II transcription subunit 12Add BLAST2190

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei80N6-acetyllysineCombined sources1
Modified residuei166PhosphotyrosineBy similarity1
Modified residuei636PhosphoserineCombined sources1
Modified residuei666PhosphoserineBy similarity1
Modified residuei699PhosphoserineBy similarity1
Modified residuei701PhosphoserineBy similarity1
Modified residuei1259PhosphoserineBy similarity1
Modified residuei1270PhosphoserineBy similarity1
Modified residuei1800N6-acetyllysineCombined sources1
Modified residuei1901Asymmetric dimethylarginine; alternateCombined sources1
Modified residuei1901Omega-N-methylarginine; alternateCombined sources1
Modified residuei1912Omega-N-methylarginineCombined sources1
Modified residuei1999Asymmetric dimethylarginineBy similarity1
Modified residuei2020Asymmetric dimethylarginineBy similarity1

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

MaxQBiA2AGH6
PaxDbiA2AGH6
PeptideAtlasiA2AGH6
PRIDEiA2AGH6

PTM databases

iPTMnetiA2AGH6
PhosphoSitePlusiA2AGH6

Expressioni

Gene expression databases

BgeeiENSMUSG00000079487 Expressed in 20 organ(s), highest expression level in bone marrow
ExpressionAtlasiA2AGH6 baseline and differential
GenevisibleiA2AGH6 MM

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Also interacts with CTNNB1 and GLI3 (By similarity).By similarity

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi208491, 1 interactor
ComplexPortaliCPX-3266 CKM complex variant 1
CPX-3267 CKM complex variant 2
CORUMiA2AGH6
DIPiDIP-59233N
IntActiA2AGH6, 11 interactors
MINTiA2AGH6
STRINGi10090.ENSMUSP00000085260

Structurei

3D structure databases

ProteinModelPortaliA2AGH6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1617 – 2063Interaction with CTNNB1 and GLI3By similarityAdd BLAST447

Sequence similaritiesi

Belongs to the Mediator complex subunit 12 family.Curated

Phylogenomic databases

eggNOGiKOG3598 Eukaryota
ENOG410XP2V LUCA
GeneTreeiENSGT00440000037505
HOGENOMiHOG000231423
HOVERGENiHBG052447
InParanoidiA2AGH6
KOiK15162
OrthoDBiEOG091G00I6
TreeFamiTF324178

Family and domain databases

InterProiView protein in InterPro
IPR019035 Mediator_Med12
IPR021989 Mediator_Med12_catenin-bd
IPR021990 Mediator_Med12_LCEWAV
PfamiView protein in Pfam
PF09497 Med12, 1 hit
PF12145 Med12-LCEWAV, 1 hit
PF12144 Med12-PQL, 1 hit
SMARTiView protein in SMART
SM01281 Med12, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: A2AGH6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAAFGILSYE HRPLKRLRLG PPDVYPQDPK QKEDELTALN VKQGFNNQPA
60 70 80 90 100
VSGDEHGSAK NVNFNPAKIS SNFSSIIAEK LRCNTLSDTG RRKSLMNQKD
110 120 130 140 150
NFWLVTARSQ SAINTWFTDL AGTKPLTHLA KKVPIFSKKE EVFGYLAKYT
160 170 180 190 200
VPVMRAAWLI KMTCAYYAAM SETKVKKKNT ADPFTEWTQI ITKYLWEQLQ
210 220 230 240 250
KMAEYYRPGP AGSGGCGSTI GPLPHDVEMA IRQWDYNEKL ALFMFQDGML
260 270 280 290 300
DRHEFLTWVL ECFEKIRPGE DELLKLLLPL LLRYSGEFVQ SAYLSRRLAY
310 320 330 340 350
FCTRRLALQL DGVSSHSSHV IAAQSTSSLP TTPAPQPPTS STPSTPFSDL
360 370 380 390 400
LMCPQHRPLV FGLSCILQTI LLCCPSALVW HYSLTDSRIK TGSPLDHLPI
410 420 430 440 450
APSNLPMPEG NSAFTQQVRA KLREIEQQIK ERGQAVEVRW SFDKCQEATA
460 470 480 490 500
GFTIGRVLHT LEVLDSHSFE RSDFSNSLDS LCNRIFGLGP SKDGHEISSD
510 520 530 540 550
DDAVVSLLCE WAVSCKRSGR HRAMVVAKLL EKRQAEIEAE RCGESEAADE
560 570 580 590 600
KGSVASGSLS APSAPIFQDV LLQFLDTQAP MLTDPRSESE RVEFFNLVLL
610 620 630 640 650
FCELIRHDVF SHNMYTCTLI SRGDLAFGAP GPRPPSPFDD PTDDPERKEA
660 670 680 690 700
EGSSSSKLED PGLSESMDID PSSSVLFEDM EKPDFSLFSP TMPCEGKGSP
710 720 730 740 750
SPEKPDVEKE VKPPAKEKIE GTLGILYDQP RHVQYATHFP IPQEESCSHE
760 770 780 790 800
CNQRLVVLFG VGKQRDDARH AIKKITKDIL KVLNRKGTAE TDQLAPIVPL
810 820 830 840 850
NPGDLTFLGG EDGQKRRRNR PEAFPTAEDI FAKFQHLSHY DQHQVTAQVS
860 870 880 890 900
RNVLEQITSF ALGMSYHLPL VQHVQFIFDL MEYSLSISGL IDFAIQLLNE
910 920 930 940 950
LSVVEAELLL KSSDLVGSYT TSLCLCIVAV LRHYHACLIL NQDQMAQVFE
960 970 980 990 1000
GLCGVVKHGM NRSDGSSAER CILAYLYDLY TSCSHLKSKF GELFSDFCSK
1010 1020 1030 1040 1050
VKNTIYCNVE PSESNMRWAP EFMIDTLENP AAHTFTYTGL GKSLSENPAN
1060 1070 1080 1090 1100
RYSFVCNALM HVCVGHHDPD RVNDIAILCA ELTGYCKSLS AEWLGVLKAL
1110 1120 1130 1140 1150
CCSSNNGTCG FNDLLCNVDV SDLSFHDSLA TFVAILIARQ CLLLEDLIRC
1160 1170 1180 1190 1200
AAIPSLLNAA CSEQDSEPGA RLTCRILLHL FKTPQLNPCQ SDGNKPTVGI
1210 1220 1230 1240 1250
RSSCDRHLLA ASQNRIVDGA VFAVLKAVFV LGDAELKGSG FTVPGGTEEL
1260 1270 1280 1290 1300
PEEEGGGGSS GRRQGGRNIS VETASLDVYA KYVLRSICQQ EWVGERCLKS
1310 1320 1330 1340 1350
LCEDSNDLQD PVLSSAQAQR LMQLICYPHR LLDNEDGENP QRQRIKRILK
1360 1370 1380 1390 1400
NLDQWTMRQS SLELQLMIKQ TPNTEMNSLL ENIAKATIEV FQQSAETGSS
1410 1420 1430 1440 1450
SGSTASNMPS SSKTKPVLSS LERSGVWLVA PLIAKLPTSV QGHVLKAAGE
1460 1470 1480 1490 1500
ELEKGQHLGS SSRKERDRQK QKSMSLLSQQ PFLSLVLTCL KGQDEQREGL
1510 1520 1530 1540 1550
LASLHSQVHQ IVINWRENQY LDDCKPKQLM HEALKLRLNL VGGMFDTVQR
1560 1570 1580 1590 1600
STQQTTEWAQ LLLEIIISGT VDMQSNNELF TTVLDMLSVL INGTLAADMS
1610 1620 1630 1640 1650
SISQGSMEEN KRAYMNLVKK LQKDLGERQS DSLEKVHQLL PLPKQNRDVI
1660 1670 1680 1690 1700
TCEPQGSLID TKGNKIAGFD SIFKKEGLQV STKQKISPWE LFEGLKPSTA
1710 1720 1730 1740 1750
PLSWAWFGTV RVDRRVARGE EQQRLLLYHT HLRPRPRAYY LEPLPLPPED
1760 1770 1780 1790 1800
EEPPAPALLE PEKKAPEPPK TDKPGAAPPS TEERKKKSTK GKKRSQPATK
1810 1820 1830 1840 1850
NEDYGMGPGR SGPYGVTVPP DLLHHANPGS ISHLSYRQSS MGLYTQNQPL
1860 1870 1880 1890 1900
PAGGPRVDPY RPVRLPMQKL PTRPTYPGVL PTTMSTVMGL EPSSYKTSVY
1910 1920 1930 1940 1950
RQQQPTVPQG QRLRQQLQAK IQSQGMLGQS SVHQMTPSSS YGLQTSQGYT
1960 1970 1980 1990 2000
SYVSHVGLQQ HTGPAGTMVP PSYSSQPYQS THPSTNPTLV DPTRHLQQRP
2010 2020 2030 2040 2050
SGYVHQQAPT YGHGLTSTQR FSHQTLQQTP MMGTMTPLSA QGVQAGVRST
2060 2070 2080 2090 2100
SILPEQQQQQ QQQQQQQQQQ QQQQQQQQQQ QQQQQQQQYH IRQQQQQQQM
2110 2120 2130 2140 2150
LRQQQQQQQQ QQQQQQQQQQ QQQQQQQQQP HQQQQQAAPP QPQPQSQPQF
2160 2170 2180 2190
QRQGLQQTQQ QQQTAALVRQ LQQQLSNTQP QPSTNIFGRY
Length:2,190
Mass (Da):244,561
Last modified:February 20, 2007 - v1
Checksum:i146E05DCCFF38DB4
GO
Isoform 2 (identifier: A2AGH6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1919-1921: Missing.
     1946-1946: S → SQLSSPSL
     1966-1990: Missing.

Note: No experimental confirmation available.
Show »
Length:2,169
Mass (Da):242,303
Checksum:i8BE79B8DEFD56844
GO
Isoform 3 (identifier: A2AGH6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1321: Missing.
     1919-1921: Missing.

Note: No experimental confirmation available.
Show »
Length:866
Mass (Da):97,885
Checksum:i4A782193143882B0
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A2AGH8A2AGH8_MOUSE
Mediator of RNA polymerase II trans...
Med12
2,157Annotation score:
A2AGH9A2AGH9_MOUSE
Mediator of RNA polymerase II trans...
Med12
2,182Annotation score:

Sequence cautioni

The sequence AAC83164 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti674S → T in AAC83164 (PubMed:15489334).Curated1
Sequence conflicti1597A → T in BAD90141 (Ref. 4) Curated1
Sequence conflicti2171L → I in AAC83164 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0299771 – 1321Missing in isoform 3. 1 PublicationAdd BLAST1321
Alternative sequenceiVSP_0299781919 – 1921Missing in isoform 2 and isoform 3. 2 Publications3
Alternative sequenceiVSP_0299791946S → SQLSSPSL in isoform 2. 1 Publication1
Alternative sequenceiVSP_0299801966 – 1990Missing in isoform 2. 1 PublicationAdd BLAST25

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL683892 Genomic DNA No translation available.
BC057085 mRNA Translation: AAH57085.1
BC057119 mRNA Translation: AAH57119.1
AF071310 mRNA Translation: AAC83164.1 Different initiation.
AK220216 mRNA Translation: BAD90141.1
CCDSiCCDS41078.1 [A2AGH6-1]
RefSeqiNP_067496.2, NM_021521.2 [A2AGH6-1]
UniGeneiMm.20873
Mm.490933

Genome annotation databases

EnsembliENSMUST00000087948; ENSMUSP00000085260; ENSMUSG00000079487 [A2AGH6-1]
ENSMUST00000087956; ENSMUSP00000085269; ENSMUSG00000079487 [A2AGH6-2]
GeneIDi59024
KEGGimmu:59024
UCSCiuc009txe.1 mouse [A2AGH6-1]
uc009txf.1 mouse [A2AGH6-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL683892 Genomic DNA No translation available.
BC057085 mRNA Translation: AAH57085.1
BC057119 mRNA Translation: AAH57119.1
AF071310 mRNA Translation: AAC83164.1 Different initiation.
AK220216 mRNA Translation: BAD90141.1
CCDSiCCDS41078.1 [A2AGH6-1]
RefSeqiNP_067496.2, NM_021521.2 [A2AGH6-1]
UniGeneiMm.20873
Mm.490933

3D structure databases

ProteinModelPortaliA2AGH6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi208491, 1 interactor
ComplexPortaliCPX-3266 CKM complex variant 1
CPX-3267 CKM complex variant 2
CORUMiA2AGH6
DIPiDIP-59233N
IntActiA2AGH6, 11 interactors
MINTiA2AGH6
STRINGi10090.ENSMUSP00000085260

PTM databases

iPTMnetiA2AGH6
PhosphoSitePlusiA2AGH6

Proteomic databases

MaxQBiA2AGH6
PaxDbiA2AGH6
PeptideAtlasiA2AGH6
PRIDEiA2AGH6

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000087948; ENSMUSP00000085260; ENSMUSG00000079487 [A2AGH6-1]
ENSMUST00000087956; ENSMUSP00000085269; ENSMUSG00000079487 [A2AGH6-2]
GeneIDi59024
KEGGimmu:59024
UCSCiuc009txe.1 mouse [A2AGH6-1]
uc009txf.1 mouse [A2AGH6-2]

Organism-specific databases

CTDi9968
MGIiMGI:1926212 Med12
RougeiSearch...

Phylogenomic databases

eggNOGiKOG3598 Eukaryota
ENOG410XP2V LUCA
GeneTreeiENSGT00440000037505
HOGENOMiHOG000231423
HOVERGENiHBG052447
InParanoidiA2AGH6
KOiK15162
OrthoDBiEOG091G00I6
TreeFamiTF324178

Enzyme and pathway databases

ReactomeiR-MMU-212436 Generic Transcription Pathway

Miscellaneous databases

ChiTaRSiMed12 mouse
PROiPR:A2AGH6
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000079487 Expressed in 20 organ(s), highest expression level in bone marrow
ExpressionAtlasiA2AGH6 baseline and differential
GenevisibleiA2AGH6 MM

Family and domain databases

InterProiView protein in InterPro
IPR019035 Mediator_Med12
IPR021989 Mediator_Med12_catenin-bd
IPR021990 Mediator_Med12_LCEWAV
PfamiView protein in Pfam
PF09497 Med12, 1 hit
PF12145 Med12-LCEWAV, 1 hit
PF12144 Med12-PQL, 1 hit
SMARTiView protein in SMART
SM01281 Med12, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMED12_MOUSE
AccessioniPrimary (citable) accession number: A2AGH6
Secondary accession number(s): A2AGH7
, O88542, Q571H3, Q6PGB4, Q6PGD8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 20, 2007
Last modified: November 7, 2018
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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