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Protein

Rhomboid-related protein 2

Gene

Rhbdl2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in regulated intramembrane proteolysis and the subsequent release of functional polypeptides from their membrane anchors. Known substrate: EFNB3 (By similarity).By similarity

Catalytic activityi

Cleaves type-1 transmembrane domains using a catalytic dyad composed of serine and histidine that are contributed by different transmembrane domains.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei186Nucleophile1
Active sitei249Curated1

GO - Molecular functioni

  • serine-type endopeptidase activity Source: UniProtKB

Keywordsi

Molecular functionHydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS54.002

Names & Taxonomyi

Protein namesi
Recommended name:
Rhomboid-related protein 2 (EC:3.4.21.105)
Short name:
RRP2
Cleaved into the following 2 chains:
Gene namesi
Name:Rhbdl2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:3608413 Rhbdl2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei71 – 91HelicalSequence analysisAdd BLAST21
Transmembranei127 – 147HelicalSequence analysisAdd BLAST21
Transmembranei158 – 178HelicalSequence analysisAdd BLAST21
Transmembranei182 – 202HelicalSequence analysisAdd BLAST21
Transmembranei211 – 231HelicalSequence analysisAdd BLAST21
Transmembranei244 – 264HelicalSequence analysisAdd BLAST21
Transmembranei277 – 297HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi185A → P: Reduces protease activity. 1 Publication1
Mutagenesisi186S → A: Abolishes protease activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000408513? – 302Rhomboid-related protein 2, C-terminal fragmentBy similarity
ChainiPRO_00004085111 – 302Rhomboid-related protein 2Add BLAST302
ChainiPRO_00004085121 – ?Rhomboid-related protein 2, N-terminal fragmentBy similarity

Post-translational modificationi

Proteolytic processing of the proenzyme produces an N- and a C-terminal fragment. The processing is required for activation of the protease (By similarity).By similarity

Proteomic databases

PaxDbiA2AGA4
PRIDEiA2AGA4

PTM databases

PhosphoSitePlusiA2AGA4

Expressioni

Gene expression databases

BgeeiENSMUSG00000043333 Expressed in 117 organ(s), highest expression level in ear
GenevisibleiA2AGA4 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000054546

Structurei

3D structure databases

ProteinModelPortaliA2AGA4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S54 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2289 Eukaryota
COG0705 LUCA
GeneTreeiENSGT00390000005726
HOGENOMiHOG000231407
HOVERGENiHBG105855
InParanoidiA2AGA4
KOiK02857
OMAiSYIAHMT
OrthoDBiEOG091G12OK
PhylomeDBiA2AGA4
TreeFamiTF313540

Family and domain databases

Gene3Di1.20.1540.10, 1 hit
InterProiView protein in InterPro
IPR002610 Peptidase_S54_rhomboid
IPR022764 Peptidase_S54_rhomboid_dom
IPR017213 Peptidase_S54_rhomboid_met
IPR035952 Rhomboid-like_sf
PANTHERiPTHR22936 PTHR22936, 1 hit
PfamiView protein in Pfam
PF01694 Rhomboid, 1 hit
PIRSFiPIRSF037470 Rhomboid, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A2AGA4-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAVAHEMEME SVNLNMEREG KEEPEEEKMK GNGEGKDFPR SRKVHRIVSK
60 70 80 90 100
WMLPEPVRRT YLERANCLPP PLFIILISLA ELAVFIYYAV WKPQKQWITL
110 120 130 140 150
DTGILESPLT YCPEKREEAW RFISYMLVHA GVQHIVGNLL MQIVLGIPLE
160 170 180 190 200
MVHKGLRVGL VYLAGVLAGS LASSIFDPLK SLVGASGGVY ALMGGYFMNV
210 220 230 240 250
IVNFREMIPA FGIVRLLVII LIVASDMGFA LYRRFFVPAN GSPVSFAAHI
260 270 280 290 300
AGGFAGMSIG YTVFSCFDKT LLKDPRFWIA IAAYVACLLF AVFFNIFLSP

AN
Length:302
Mass (Da):33,735
Last modified:February 20, 2007 - v1
Checksum:i3623D9206D486C57
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL683811 Genomic DNA No translation available.
CCDSiCCDS38873.1
RefSeqiNP_898986.2, NM_183163.2
XP_006503088.1, XM_006503025.3
UniGeneiMm.37334

Genome annotation databases

EnsembliENSMUST00000053202; ENSMUSP00000054546; ENSMUSG00000043333
ENSMUST00000106204; ENSMUSP00000101810; ENSMUSG00000043333
GeneIDi230726
KEGGimmu:230726
UCSCiuc008upx.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL683811 Genomic DNA No translation available.
CCDSiCCDS38873.1
RefSeqiNP_898986.2, NM_183163.2
XP_006503088.1, XM_006503025.3
UniGeneiMm.37334

3D structure databases

ProteinModelPortaliA2AGA4
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000054546

Protein family/group databases

MEROPSiS54.002

PTM databases

PhosphoSitePlusiA2AGA4

Proteomic databases

PaxDbiA2AGA4
PRIDEiA2AGA4

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000053202; ENSMUSP00000054546; ENSMUSG00000043333
ENSMUST00000106204; ENSMUSP00000101810; ENSMUSG00000043333
GeneIDi230726
KEGGimmu:230726
UCSCiuc008upx.1 mouse

Organism-specific databases

CTDi54933
MGIiMGI:3608413 Rhbdl2

Phylogenomic databases

eggNOGiKOG2289 Eukaryota
COG0705 LUCA
GeneTreeiENSGT00390000005726
HOGENOMiHOG000231407
HOVERGENiHBG105855
InParanoidiA2AGA4
KOiK02857
OMAiSYIAHMT
OrthoDBiEOG091G12OK
PhylomeDBiA2AGA4
TreeFamiTF313540

Miscellaneous databases

PROiPR:A2AGA4
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000043333 Expressed in 117 organ(s), highest expression level in ear
GenevisibleiA2AGA4 MM

Family and domain databases

Gene3Di1.20.1540.10, 1 hit
InterProiView protein in InterPro
IPR002610 Peptidase_S54_rhomboid
IPR022764 Peptidase_S54_rhomboid_dom
IPR017213 Peptidase_S54_rhomboid_met
IPR035952 Rhomboid-like_sf
PANTHERiPTHR22936 PTHR22936, 1 hit
PfamiView protein in Pfam
PF01694 Rhomboid, 1 hit
PIRSFiPIRSF037470 Rhomboid, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRHBL2_MOUSE
AccessioniPrimary (citable) accession number: A2AGA4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 31, 2011
Last sequence update: February 20, 2007
Last modified: November 7, 2018
This is version 86 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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