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Entry version 89 (13 Feb 2019)
Sequence version 1 (20 Feb 2007)
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Protein

tRNA pseudouridine synthase-like 1

Gene

Pusl1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Function’ section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei66NucleophileBy similarity1
<p>This subsection of the ‘Function’ section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei130SubstrateBy similarity1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

  • pseudouridine synthase activity Source: GO_Central
  • RNA binding Source: InterPro

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionIsomerase
Biological processtRNA processing

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
tRNA pseudouridine synthase-like 1 (EC:5.4.99.-)
Alternative name(s):
tRNA pseudouridylate synthase-like 1
tRNA-uridine isomerase-like 1
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:Pusl1
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiMus musculus (Mouse)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri10090 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000589 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 4

Organism-specific databases

Mouse genome database (MGD) from Mouse Genome Informatics (MGI)

More...
MGIi
MGI:3047787 Pusl1

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00003583161 – 291tRNA pseudouridine synthase-like 1Add BLAST291

Proteomic databases

Encyclopedia of Proteome Dynamics

More...
EPDi
A2ADA5

MaxQB - The MaxQuant DataBase

More...
MaxQBi
A2ADA5

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
A2ADA5

PeptideAtlas

More...
PeptideAtlasi
A2ADA5

PRoteomics IDEntifications database

More...
PRIDEi
A2ADA5

PTM databases

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...
PhosphoSitePlusi
A2ADA5

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...
Bgeei
ENSMUSG00000051557 Expressed in 20 organ(s), highest expression level in Ammon's horn

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...
BioGridi
241456, 1 interactor

STRING: functional protein association networks

More...
STRINGi
10090.ENSMUSP00000095344

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

Protein Model Portal of the PSI-Nature Structural Biology Knowledgebase

More...
ProteinModelPortali
A2ADA5

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
A2ADA5

Database of comparative protein structure models

More...
ModBasei
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
KOG4393 Eukaryota
COG0101 LUCA

Ensembl GeneTree

More...
GeneTreei
ENSGT00940000153884

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
HOG000248674

The HOVERGEN Database of Homologous Vertebrate Genes

More...
HOVERGENi
HBG053770

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
A2ADA5

Identification of Orthologs from Complete Genome Data

More...
OMAi
FLYGMVR

Database of Orthologous Groups

More...
OrthoDBi
831280at2759

Database for complete collections of gene phylogenies

More...
PhylomeDBi
A2ADA5

TreeFam database of animal gene trees

More...
TreeFami
TF105127

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
3.30.70.660, 1 hit

HAMAP database of protein families

More...
HAMAPi
MF_00171 TruA, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR020103 PsdUridine_synth_cat_dom_sf
IPR001406 PsdUridine_synth_TruA
IPR020097 PsdUridine_synth_TruA_a/b_dom
IPR020095 PsdUridine_synth_TruA_C

The PANTHER Classification System

More...
PANTHERi
PTHR11142 PTHR11142, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF01416 PseudoU_synth_1, 2 hits

PIRSF; a whole-protein classification database

More...
PIRSFi
PIRSF001430 tRNA_psdUrid_synth, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF55120 SSF55120, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (3)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 3 <p>This subsection of the ‘Sequence’ section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: A2ADA5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGSCGAVGSV RARYLVFLQY LGTDFNGVAA VRGNPRAVGV LNFLEEAAKR
60 70 80 90 100
LNSVDPVRFT ISSRTDAGVH ALSNAAHLDI QRRPGQSPFS PEVVAKALNT
110 120 130 140 150
HLKHPAIRVL KAFRVPNDFH ARHAATSRTY QYRLATGCSW PNQLPVFEQN
160 170 180 190 200
VCWALQTEYL DMAAMQEAAQ HLLGTHDFSA FQSAGSPVTN TVRTLRRVSV
210 220 230 240 250
SPGPASPFVL PEGSRRLQFW TLEFESQSFL YRQVRRMTAV LVAVGLGILA
260 270 280 290
PTQVKVILES QDPLGKYQAR VAPARGLFLK SVLYDNFGPT S
Length:291
Mass (Da):31,993
Last modified:February 20, 2007 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iC7013FBEE8318494
GO
Isoform 2 (identifier: A2ADA5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-35: Missing.
     36-45: RAVGVLNFLE → MWQPPVRFPQ
     232-232: R → RQVGCLNPHRMGSGRHTCDNLSSLL

Show »
Length:280
Mass (Da):31,110
Checksum:i8A5D0E710D223DF8
GO
Isoform 3 (identifier: A2ADA5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-65: Missing.
     66-69: DAGV → MPGL
     266-291: KYQARVAPARGLFLKSVLYDNFGPTS → QVSGSSCSSPWPVLEVSAV

Show »
Length:219
Mass (Da):24,111
Checksum:i63957BDF70991B7C
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_0360491 – 65Missing in isoform 3. 1 PublicationAdd BLAST65
Alternative sequenceiVSP_0360501 – 35Missing in isoform 2. 1 PublicationAdd BLAST35
Alternative sequenceiVSP_03605136 – 45RAVGVLNFLE → MWQPPVRFPQ in isoform 2. 1 Publication10
Alternative sequenceiVSP_03605266 – 69DAGV → MPGL in isoform 3. 1 Publication4
Alternative sequenceiVSP_036053232R → RQVGCLNPHRMGSGRHTCDN LSSLL in isoform 2. 1 Publication1
Alternative sequenceiVSP_036054266 – 291KYQAR…FGPTS → QVSGSSCSSPWPVLEVSAV in isoform 3. 1 PublicationAdd BLAST26

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AK151452 mRNA Translation: BAE30412.1
AK160236 mRNA Translation: BAE35706.1
AL670236 Genomic DNA No translation available.

The Consensus CDS (CCDS) project

More...
CCDSi
CCDS51404.1 [A2ADA5-1]

NCBI Reference Sequences

More...
RefSeqi
NP_001028662.1, NM_001033490.1 [A2ADA5-1]
XP_006539075.1, XM_006539012.1 [A2ADA5-2]

UniGene gene-oriented nucleotide sequence clusters

More...
UniGenei
Mm.259270

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...
Ensembli
ENSMUST00000097737; ENSMUSP00000095344; ENSMUSG00000051557 [A2ADA5-1]

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
433813

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
mmu:433813

UCSC genome browser

More...
UCSCi
uc008wfi.1 mouse [A2ADA5-2]
uc012drb.1 mouse [A2ADA5-1]

Keywords - Coding sequence diversityi

Alternative splicing

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK151452 mRNA Translation: BAE30412.1
AK160236 mRNA Translation: BAE35706.1
AL670236 Genomic DNA No translation available.
CCDSiCCDS51404.1 [A2ADA5-1]
RefSeqiNP_001028662.1, NM_001033490.1 [A2ADA5-1]
XP_006539075.1, XM_006539012.1 [A2ADA5-2]
UniGeneiMm.259270

3D structure databases

ProteinModelPortaliA2ADA5
SMRiA2ADA5
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi241456, 1 interactor
STRINGi10090.ENSMUSP00000095344

PTM databases

PhosphoSitePlusiA2ADA5

Proteomic databases

EPDiA2ADA5
MaxQBiA2ADA5
PaxDbiA2ADA5
PeptideAtlasiA2ADA5
PRIDEiA2ADA5

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000097737; ENSMUSP00000095344; ENSMUSG00000051557 [A2ADA5-1]
GeneIDi433813
KEGGimmu:433813
UCSCiuc008wfi.1 mouse [A2ADA5-2]
uc012drb.1 mouse [A2ADA5-1]

Organism-specific databases

Comparative Toxicogenomics Database

More...
CTDi
126789
MGIiMGI:3047787 Pusl1

Phylogenomic databases

eggNOGiKOG4393 Eukaryota
COG0101 LUCA
GeneTreeiENSGT00940000153884
HOGENOMiHOG000248674
HOVERGENiHBG053770
InParanoidiA2ADA5
OMAiFLYGMVR
OrthoDBi831280at2759
PhylomeDBiA2ADA5
TreeFamiTF105127

Miscellaneous databases

Protein Ontology

More...
PROi
PR:A2ADA5

The Stanford Online Universal Resource for Clones and ESTs

More...
SOURCEi
Search...

Gene expression databases

BgeeiENSMUSG00000051557 Expressed in 20 organ(s), highest expression level in Ammon's horn

Family and domain databases

Gene3Di3.30.70.660, 1 hit
HAMAPiMF_00171 TruA, 1 hit
InterProiView protein in InterPro
IPR020103 PsdUridine_synth_cat_dom_sf
IPR001406 PsdUridine_synth_TruA
IPR020097 PsdUridine_synth_TruA_a/b_dom
IPR020095 PsdUridine_synth_TruA_C
PANTHERiPTHR11142 PTHR11142, 1 hit
PfamiView protein in Pfam
PF01416 PseudoU_synth_1, 2 hits
PIRSFiPIRSF001430 tRNA_psdUrid_synth, 1 hit
SUPFAMiSSF55120 SSF55120, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiPUSL1_MOUSE
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: A2ADA5
Secondary accession number(s): Q3TVB3, Q3UAA3
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 16, 2008
Last sequence update: February 20, 2007
Last modified: February 13, 2019
This is version 89 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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