UniProtKB - A2ABV5 (MED14_MOUSE)
Mediator of RNA polymerase II transcription subunit 14
Med14
Functioni
GO - Molecular functioni
- transcription coactivator activity Source: MGI
- transcription coregulator activity Source: MGI
GO - Biological processi
- positive regulation of transcription, DNA-templated Source: MGI
- positive regulation of transcription by RNA polymerase II Source: MGI
- positive regulation of transcription initiation from RNA polymerase II promoter Source: MGI
- regulation of transcription by RNA polymerase II Source: GO_Central
- stem cell population maintenance Source: MGI
Keywordsi
Molecular function | Activator |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-MMU-212436, Generic Transcription Pathway |
Names & Taxonomyi
Protein namesi | Recommended name: Mediator of RNA polymerase II transcription subunit 14Alternative name(s): Cofactor required for Sp1 transcriptional activation subunit 2 Short name: CRSP complex subunit 2 Mediator complex subunit 14 Thyroid hormone receptor-associated protein complex 170 kDa component Short name: Trap170 |
Gene namesi | Name:Med14 Synonyms:Crsp2, Gm641, Trap170 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1349442, Med14 |
Subcellular locationi
Nucleus
- Nucleus Curated
Nucleus
- core mediator complex Source: GO_Central
- mediator complex Source: MGI
- nucleoplasm Source: Reactome
- nucleus Source: MGI
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000304585 | 1 – 1459 | Mediator of RNA polymerase II transcription subunit 14Add BLAST | 1459 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 623 | PhosphoserineBy similarity | 1 | |
Modified residuei | 992 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1117 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1124 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1133 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1141 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1149 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | A2ABV5 |
MaxQBi | A2ABV5 |
PaxDbi | A2ABV5 |
PeptideAtlasi | A2ABV5 |
PRIDEi | A2ABV5 |
PTM databases
iPTMneti | A2ABV5 |
PhosphoSitePlusi | A2ABV5 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000064127, Expressed in ear and 309 other tissues |
ExpressionAtlasi | A2ABV5, baseline and differential |
Genevisiblei | A2ABV5, MM |
Interactioni
Subunit structurei
Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.
Interacts with AR, ESR1, SREBF1 and STAT2 (By similarity).
Interacts with GATA1.
By similarity1 PublicationProtein-protein interaction databases
BioGRIDi | 205047, 5 interactors |
ComplexPortali | CPX-3264, Core mediator complex |
CORUMi | A2ABV5 |
IntActi | A2ABV5, 4 interactors |
MINTi | A2ABV5 |
STRINGi | 10090.ENSMUSP00000094239 |
Miscellaneous databases
RNActi | A2ABV5, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 194 – 572 | Interaction with STAT2By similarityAdd BLAST | 379 | |
Regioni | 506 – 830 | Interaction with SREBF1By similarityAdd BLAST | 325 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 75 – 79 | LXXLL motif 1 | 5 | |
Motifi | 1187 – 1191 | LXXLL motif 2 | 5 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 31 – 51 | Ala-richAdd BLAST | 21 | |
Compositional biasi | 1008 – 1170 | Pro-richAdd BLAST | 163 |
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG1875, Eukaryota |
GeneTreei | ENSGT00390000001021 |
HOGENOMi | CLU_001928_0_0_1 |
InParanoidi | A2ABV5 |
OMAi | HLDCPIT |
OrthoDBi | 1215335at2759 |
PhylomeDBi | A2ABV5 |
TreeFami | TF314388 |
Family and domain databases
InterProi | View protein in InterPro IPR013947, Mediator_Med14 |
PANTHERi | PTHR12809, PTHR12809, 1 hit |
Pfami | View protein in Pfam PF08638, Med14, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAPVQLDNHQ LIPPGGGGGS SGGGGSSSGS ASAPAPPPPA AAVAAAAAAA
60 70 80 90 100
ASPGYRLSTL IEFLLHRAYS ELMVLTDLLP RKSDVERKIE IVQFASRTRQ
110 120 130 140 150
LFVRLLALVK WANDAGKVEK CAMISSFLDQ QAILFVDTAD RLASLARDAL
160 170 180 190 200
VHARLPSFAI PYAIDVLTTG SYPRLPTCIR DKIIPPDPIT KIEKQATLHQ
210 220 230 240 250
LNQILRHRLV TTDLPPQLAN LTVANGRVKF RVEGEFEATL TVMGDDPEVP
260 270 280 290 300
WRLLKLEILV EDKETGDGRA LVHSMQIDFI HQLVQSRLFA DEKPLQDMYN
310 320 330 340 350
CLHCFCLSLQ LEVLHSQTLM LIRERWGDLV QVERYHAGKS LSLSVWNQQV
360 370 380 390 400
LGRKTGTASV HKVTIKIDEN DVSKPLQIFH DPPLPASDSK LVERAMKIDH
410 420 430 440 450
LSIEKLLIDS VHARAHQRLQ ELKAILRSFN ANESSSIETA LPALIVPILE
460 470 480 490 500
PCGNSECLHI FVDLHSGMFQ LMLYGLDPAT LEDMEKSLND DMKRIIPWIQ
510 520 530 540 550
QLKFWLGQQR CKQSIKHLPT ITTETLQLAN YSTHPIGSLS KNKLFIKLTR
560 570 580 590 600
LPQYYIVVEM LEVPNKPTQL SYNYYFMSVS TADREDSPVM ALLLQQFKDN
610 620 630 640 650
IQDLMSYTKT GKQTRTGTKH KLSDDPCPID SKKAKRSGEM CAFNKVLAHF
660 670 680 690 700
VAMCDTNMPF VGLRLELSNL EIPHQGVQVE GDGFNHAIRL LKIPPCKGIS
710 720 730 740 750
EETQKALDRS LLDCTFRLQG RNNRTWVAEL VFANCPLNGT STREQGPSRH
760 770 780 790 800
VYLTYENLLS EPVGGRKVVE MFLNDWSSIA RLYECVLEFA RSLPEIPAHL
810 820 830 840 850
NIFSEVRVYN YRKLILCYGT TKGSSISIQW NSIHQKFHIA LGTVGPNSGC
860 870 880 890 900
SNCHNTILHQ LQEMFNKTPN VVQLLQVLFD TQAPLNAINK LPTVPMLGLT
910 920 930 940 950
QRTNTAYQCF SILPQSSTHI RLAFRNMYCI DIYCRSRGVV AIRDGAYSLF
960 970 980 990 1000
DNSKLVEGFY PAPGLKTFLN MFVDSNQDAR RRSVNEDDNP PSPIGGDMMD
1010 1020 1030 1040 1050
SLISQLQPPQ QQPFPKQPGT SGAYPLTSPP TSYHSTVNQS PSMMHTQSPG
1060 1070 1080 1090 1100
NLHAASSPSG ALRAPSPASF VPTPPPSSHG ISIGPGASFA SPHGTLDPSS
1110 1120 1130 1140 1150
PYTMVSPSGR AGNWPGSPQV SGPSPATRLP GMSPANPSLH SPVPDVSHSP
1160 1170 1180 1190 1200
RAGTSSQTMP TNMPPPRKLP QRSWAASIPT ILTHSALNIL LLPSPTPGLV
1210 1220 1230 1240 1250
PGLAGSYLCS PLERFLGSVI MRRHLQRIIQ QETLQLINSN EPGVIMFKTD
1260 1270 1280 1290 1300
ALKCRVALSP KTNQTLQLKV TPENAGQWKP DELQVLEKFF ETRVAGPPFK
1310 1320 1330 1340 1350
ANTLIAFTKL LGAPTHILRD CVHIMKLELF PDQATQLKWN VQFCLTIPPS
1360 1370 1380 1390 1400
APPIAPPGTP AVVLKSKMLF FLQLTQKTSV PPQEPVSIIV PIIYDMASGT
1410 1420 1430 1440 1450
TQQADIPRQQ NSSVAAPMMV SNILKRFAEM NPPRQGECTI FAAVRDLMAN
LTLPPGGRP
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA2BDN7 | A2BDN7_MOUSE | Mediator of RNA polymerase II trans... | Med14 | 700 | Annotation score: | ||
A2BDP0 | A2BDP0_MOUSE | Mediator of RNA polymerase II trans... | Med14 | 798 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 158 | F → C in BAE37289 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 346 – 347 | WN → C in BAA76611 (PubMed:10512684).Curated | 2 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_028033 | 1 – 72 | Missing in isoform 4. 1 PublicationAdd BLAST | 72 | |
Alternative sequenceiVSP_028034 | 435 – 1459 | Missing in isoform 4. 1 PublicationAdd BLAST | 1025 | |
Alternative sequenceiVSP_028035 | 436 – 471 | SIETA…GMFQL → KHSRAGEMAQQLRVPTALPK VLSSNPINHMVAHNHL in isoform 3. 1 PublicationAdd BLAST | 36 | |
Alternative sequenceiVSP_028036 | 472 – 1459 | Missing in isoform 3. 1 PublicationAdd BLAST | 988 | |
Alternative sequenceiVSP_028037 | 747 – 770 | PSRHV…RKVVE → KIFHTVFLLMSIFLKVLVTI SFFL in isoform 2. 1 PublicationAdd BLAST | 24 | |
Alternative sequenceiVSP_028038 | 771 – 1459 | Missing in isoform 2. 1 PublicationAdd BLAST | 689 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB019028 mRNA Translation: BAA76610.1 AB019029 Genomic DNA Translation: BAA76611.1 AK163306 mRNA Translation: BAE37289.1 AL662925 Genomic DNA No translation available. BX000537 Genomic DNA No translation available. BC065072 mRNA Translation: AAH65072.1 |
CCDSi | CCDS40874.1 [A2ABV5-1] |
RefSeqi | NP_001041673.1, NM_001048208.1 [A2ABV5-1] NP_036135.3, NM_012005.3 |
Genome annotation databases
Ensembli | ENSMUST00000096495; ENSMUSP00000094239; ENSMUSG00000064127 [A2ABV5-1] |
GeneIDi | 26896 |
KEGGi | mmu:26896 |
UCSCi | uc009sqz.1, mouse [A2ABV5-1] uc009srd.1, mouse [A2ABV5-3] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB019028 mRNA Translation: BAA76610.1 AB019029 Genomic DNA Translation: BAA76611.1 AK163306 mRNA Translation: BAE37289.1 AL662925 Genomic DNA No translation available. BX000537 Genomic DNA No translation available. BC065072 mRNA Translation: AAH65072.1 |
CCDSi | CCDS40874.1 [A2ABV5-1] |
RefSeqi | NP_001041673.1, NM_001048208.1 [A2ABV5-1] NP_036135.3, NM_012005.3 |
3D structure databases
SMRi | A2ABV5 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 205047, 5 interactors |
ComplexPortali | CPX-3264, Core mediator complex |
CORUMi | A2ABV5 |
IntActi | A2ABV5, 4 interactors |
MINTi | A2ABV5 |
STRINGi | 10090.ENSMUSP00000094239 |
PTM databases
iPTMneti | A2ABV5 |
PhosphoSitePlusi | A2ABV5 |
Proteomic databases
EPDi | A2ABV5 |
MaxQBi | A2ABV5 |
PaxDbi | A2ABV5 |
PeptideAtlasi | A2ABV5 |
PRIDEi | A2ABV5 |
Protocols and materials databases
Antibodypediai | 10773, 154 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000096495; ENSMUSP00000094239; ENSMUSG00000064127 [A2ABV5-1] |
GeneIDi | 26896 |
KEGGi | mmu:26896 |
UCSCi | uc009sqz.1, mouse [A2ABV5-1] uc009srd.1, mouse [A2ABV5-3] |
Organism-specific databases
CTDi | 9282 |
MGIi | MGI:1349442, Med14 |
Phylogenomic databases
eggNOGi | KOG1875, Eukaryota |
GeneTreei | ENSGT00390000001021 |
HOGENOMi | CLU_001928_0_0_1 |
InParanoidi | A2ABV5 |
OMAi | HLDCPIT |
OrthoDBi | 1215335at2759 |
PhylomeDBi | A2ABV5 |
TreeFami | TF314388 |
Enzyme and pathway databases
Reactomei | R-MMU-212436, Generic Transcription Pathway |
Miscellaneous databases
BioGRID-ORCSi | 26896, 7 hits in 16 CRISPR screens |
ChiTaRSi | Med14, mouse |
PROi | PR:A2ABV5 |
RNActi | A2ABV5, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000064127, Expressed in ear and 309 other tissues |
ExpressionAtlasi | A2ABV5, baseline and differential |
Genevisiblei | A2ABV5, MM |
Family and domain databases
InterProi | View protein in InterPro IPR013947, Mediator_Med14 |
PANTHERi | PTHR12809, PTHR12809, 1 hit |
Pfami | View protein in Pfam PF08638, Med14, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MED14_MOUSE | |
Accessioni | A2ABV5Primary (citable) accession number: A2ABV5 Secondary accession number(s): Q3TQU3 Q9WTN9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 11, 2007 |
Last sequence update: | February 20, 2007 | |
Last modified: | December 2, 2020 | |
This is version 111 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families