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UniProtKB - A2ABV5 (MED14_MOUSE)

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Protein

Mediator of RNA polymerase II transcription subunit 14

Gene

Med14

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity).By similarity

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

GO - Biological processi

View the complete GO annotation on QuickGO ...

Keywordsi

Molecular functionActivator
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-212436 Generic Transcription Pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 14
Alternative name(s):
Cofactor required for Sp1 transcriptional activation subunit 2
Short name:
CRSP complex subunit 2
Mediator complex subunit 14
Thyroid hormone receptor-associated protein complex 170 kDa component
Short name:
Trap170
Gene namesi
Name:Med14
Synonyms:Crsp2, Gm641, Trap170
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:1349442 Med14

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003045851 – 1459Mediator of RNA polymerase II transcription subunit 14Add BLAST1459

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei623PhosphoserineBy similarity1
Modified residuei992PhosphoserineBy similarity1
Modified residuei1117PhosphoserineCombined sources1
Modified residuei1124PhosphoserineCombined sources1
Modified residuei1133PhosphoserineCombined sources1
Modified residuei1141PhosphoserineCombined sources1
Modified residuei1149PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiA2ABV5
MaxQBiA2ABV5
PaxDbiA2ABV5
PeptideAtlasiA2ABV5
PRIDEiA2ABV5

PTM databases

iPTMnetiA2ABV5
PhosphoSitePlusiA2ABV5

Expressioni

Gene expression databases

BgeeiENSMUSG00000064127
CleanExiMM_MED14
ExpressionAtlasiA2ABV5 baseline and differential
GenevisibleiA2ABV5 MM

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Interacts with AR, ESR1, SREBF1 and STAT2 (By similarity). Interacts with GATA1.By similarity1 Publication

Protein-protein interaction databases

BioGridi205047, 5 interactors
ComplexPortaliCPX-3264 Core mediator complex
CORUMiA2ABV5
IntActiA2ABV5, 4 interactors
MINTiA2ABV5
STRINGi10090.ENSMUSP00000094239

Structurei

3D structure databases

ProteinModelPortaliA2ABV5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni194 – 572Interaction with STAT2By similarityAdd BLAST379
Regioni506 – 830Interaction with SREBF1By similarityAdd BLAST325

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi75 – 79LXXLL motif 15
Motifi1187 – 1191LXXLL motif 25

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi31 – 51Ala-richAdd BLAST21
Compositional biasi1008 – 1170Pro-richAdd BLAST163

Sequence similaritiesi

Belongs to the Mediator complex subunit 14 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1875 Eukaryota
ENOG410XWK4 LUCA
GeneTreeiENSGT00390000001021
HOGENOMiHOG000045361
HOVERGENiHBG104308
InParanoidiA2ABV5
KOiK15156
OMAiCHNTILH
OrthoDBiEOG091G01FK
PhylomeDBiA2ABV5
TreeFamiTF314388

Family and domain databases

InterProiView protein in InterPro
IPR013947 Mediator_Med14
PfamiView protein in Pfam
PF08638 Med14, 1 hit

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: A2ABV5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAPVQLDNHQ LIPPGGGGGS SGGGGSSSGS ASAPAPPPPA AAVAAAAAAA 
        60         70         80         90        100
ASPGYRLSTL IEFLLHRAYS ELMVLTDLLP RKSDVERKIE IVQFASRTRQ 
       110        120        130        140        150
LFVRLLALVK WANDAGKVEK CAMISSFLDQ QAILFVDTAD RLASLARDAL 
       160        170        180        190        200
VHARLPSFAI PYAIDVLTTG SYPRLPTCIR DKIIPPDPIT KIEKQATLHQ 
       210        220        230        240        250
LNQILRHRLV TTDLPPQLAN LTVANGRVKF RVEGEFEATL TVMGDDPEVP 
       260        270        280        290        300
WRLLKLEILV EDKETGDGRA LVHSMQIDFI HQLVQSRLFA DEKPLQDMYN 
       310        320        330        340        350
CLHCFCLSLQ LEVLHSQTLM LIRERWGDLV QVERYHAGKS LSLSVWNQQV 
       360        370        380        390        400
LGRKTGTASV HKVTIKIDEN DVSKPLQIFH DPPLPASDSK LVERAMKIDH 
       410        420        430        440        450
LSIEKLLIDS VHARAHQRLQ ELKAILRSFN ANESSSIETA LPALIVPILE 
       460        470        480        490        500
PCGNSECLHI FVDLHSGMFQ LMLYGLDPAT LEDMEKSLND DMKRIIPWIQ 
       510        520        530        540        550
QLKFWLGQQR CKQSIKHLPT ITTETLQLAN YSTHPIGSLS KNKLFIKLTR 
       560        570        580        590        600
LPQYYIVVEM LEVPNKPTQL SYNYYFMSVS TADREDSPVM ALLLQQFKDN 
       610        620        630        640        650
IQDLMSYTKT GKQTRTGTKH KLSDDPCPID SKKAKRSGEM CAFNKVLAHF 
       660        670        680        690        700
VAMCDTNMPF VGLRLELSNL EIPHQGVQVE GDGFNHAIRL LKIPPCKGIS 
       710        720        730        740        750
EETQKALDRS LLDCTFRLQG RNNRTWVAEL VFANCPLNGT STREQGPSRH 
       760        770        780        790        800
VYLTYENLLS EPVGGRKVVE MFLNDWSSIA RLYECVLEFA RSLPEIPAHL 
       810        820        830        840        850
NIFSEVRVYN YRKLILCYGT TKGSSISIQW NSIHQKFHIA LGTVGPNSGC 
       860        870        880        890        900
SNCHNTILHQ LQEMFNKTPN VVQLLQVLFD TQAPLNAINK LPTVPMLGLT 
       910        920        930        940        950
QRTNTAYQCF SILPQSSTHI RLAFRNMYCI DIYCRSRGVV AIRDGAYSLF 
       960        970        980        990       1000
DNSKLVEGFY PAPGLKTFLN MFVDSNQDAR RRSVNEDDNP PSPIGGDMMD 
      1010       1020       1030       1040       1050
SLISQLQPPQ QQPFPKQPGT SGAYPLTSPP TSYHSTVNQS PSMMHTQSPG 
      1060       1070       1080       1090       1100
NLHAASSPSG ALRAPSPASF VPTPPPSSHG ISIGPGASFA SPHGTLDPSS 
      1110       1120       1130       1140       1150
PYTMVSPSGR AGNWPGSPQV SGPSPATRLP GMSPANPSLH SPVPDVSHSP 
      1160       1170       1180       1190       1200
RAGTSSQTMP TNMPPPRKLP QRSWAASIPT ILTHSALNIL LLPSPTPGLV 
      1210       1220       1230       1240       1250
PGLAGSYLCS PLERFLGSVI MRRHLQRIIQ QETLQLINSN EPGVIMFKTD 
      1260       1270       1280       1290       1300
ALKCRVALSP KTNQTLQLKV TPENAGQWKP DELQVLEKFF ETRVAGPPFK 
      1310       1320       1330       1340       1350
ANTLIAFTKL LGAPTHILRD CVHIMKLELF PDQATQLKWN VQFCLTIPPS 
      1360       1370       1380       1390       1400
APPIAPPGTP AVVLKSKMLF FLQLTQKTSV PPQEPVSIIV PIIYDMASGT 
      1410       1420       1430       1440       1450
TQQADIPRQQ NSSVAAPMMV SNILKRFAEM NPPRQGECTI FAAVRDLMAN 

LTLPPGGRP
Length:1,459
Mass (Da):160,966
Last modified:February 20, 2007 - v1
Checksum:iFE61E45E8E99E4E9
GO
Isoform 2 (identifier: A2ABV5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     747-770: PSRHVYLTYENLLSEPVGGRKVVE → KIFHTVFLLMSIFLKVLVTISFFL
     771-1459: Missing.

Note: No experimental confirmation available.
Show »
Length:770
Mass (Da):86,050
Checksum:iAE645E84E4B373E2
GO
Isoform 3 (identifier: A2ABV5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     436-471: SIETALPALIVPILEPCGNSECLHIFVDLHSGMFQL → KHSRAGEMAQQLRVPTALPKVLSSNPINHMVAHNHL
     472-1459: Missing.

Show »
Length:471
Mass (Da):51,942
Checksum:i9E560273E9D3CC85
GO
Isoform 4 (identifier: A2ABV5-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-72: Missing.
     435-1459: Missing.

Note: No experimental confirmation available.
Show »
Length:362
Mass (Da):41,127
Checksum:i2F6672DA26CFE08C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti158F → C in BAE37289 (PubMed:16141072).Curated1
Sequence conflicti346 – 347WN → C in BAA76611 (PubMed:10512684).Curated2

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0280331 – 72Missing in isoform 4. 1 PublicationAdd BLAST72
Alternative sequenceiVSP_028034435 – 1459Missing in isoform 4. 1 PublicationAdd BLAST1025
Alternative sequenceiVSP_028035436 – 471SIETA…GMFQL → KHSRAGEMAQQLRVPTALPK VLSSNPINHMVAHNHL in isoform 3. 1 PublicationAdd BLAST36
Alternative sequenceiVSP_028036472 – 1459Missing in isoform 3. 1 PublicationAdd BLAST988
Alternative sequenceiVSP_028037747 – 770PSRHV…RKVVE → KIFHTVFLLMSIFLKVLVTI SFFL in isoform 2. 1 PublicationAdd BLAST24
Alternative sequenceiVSP_028038771 – 1459Missing in isoform 2. 1 PublicationAdd BLAST689

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB019028 mRNA Translation: BAA76610.1
AB019029 Genomic DNA Translation: BAA76611.1
AK163306 mRNA Translation: BAE37289.1
AL662925, BX000537 Genomic DNA Translation: CAM17981.1
BX000537, AL662925 Genomic DNA Translation: CAM27890.1
BC065072 mRNA Translation: AAH65072.1
CCDSiCCDS40874.1 [A2ABV5-1]
RefSeqiNP_001041673.1, NM_001048208.1 [A2ABV5-1]
NP_036135.3, NM_012005.3
UniGeneiMm.17616

Genome annotation databases

EnsembliENSMUST00000096495; ENSMUSP00000094239; ENSMUSG00000064127 [A2ABV5-1]
GeneIDi26896
KEGGimmu:26896
UCSCiuc009sqz.1 mouse [A2ABV5-1]
uc009srd.1 mouse [A2ABV5-3]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMED14_MOUSE
AccessioniPrimary (citable) accession number: A2ABV5
Secondary accession number(s): Q3TQU3
, Q6P1H5, Q9R0T1, Q9WTN9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: February 20, 2007
Last modified: July 18, 2018
This is version 98 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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