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Protein

Rho GTPase-activating protein 27

Gene

Arhgap27

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Rho GTPase-activating protein which may be involved in clathrin-mediated endocytosis. GTPase activators for the Rho-type GTPases act by converting them to an inactive GDP-bound state. Has activity toward CDC42 and RAC1 (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGTPase activation
Biological processEndocytosis

Enzyme and pathway databases

ReactomeiR-MMU-194840 Rho GTPase cycle

Names & Taxonomyi

Protein namesi
Recommended name:
Rho GTPase-activating protein 27
Alternative name(s):
CIN85-associated multi-domain-containing Rho GTPase-activating protein 1
Rho-type GTPase-activating protein 27
Gene namesi
Name:Arhgap27
Synonyms:Camgap1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1916903 Arhgap27

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003175791 – 869Rho GTPase-activating protein 27Add BLAST869

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei155PhosphoserineBy similarity1
Modified residuei215PhosphoserineCombined sources1
Modified residuei249PhosphoserineBy similarity1
Modified residuei350PhosphoserineCombined sources1
Modified residuei459PhosphoserineCombined sources1
Modified residuei462PhosphoserineCombined sources1
Modified residuei464PhosphothreonineCombined sources1
Modified residuei469PhosphoserineCombined sources1
Modified residuei632PhosphoserineCombined sources1
Modified residuei636PhosphoserineCombined sources1
Isoform 2 (identifier: A2AB59-2)
Modified residuei28PhosphotyrosineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiA2AB59
MaxQBiA2AB59
PaxDbiA2AB59
PeptideAtlasiA2AB59
PRIDEiA2AB59

PTM databases

iPTMnetiA2AB59
PhosphoSitePlusiA2AB59

Expressioni

Tissue specificityi

Widely expressed. Highly expressed in kidney, lung, small intestine and thymus.1 Publication

Gene expression databases

BgeeiENSMUSG00000034255 Expressed in 190 organ(s), highest expression level in pyloric antrum
CleanExiMM_ARHGAP27
ExpressionAtlasiA2AB59 baseline and differential
GenevisibleiA2AB59 MM

Interactioni

Subunit structurei

Interacts with SH3KBP1/CIN85.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000102639

Structurei

3D structure databases

ProteinModelPortaliA2AB59
SMRiA2AB59
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 69SH3PROSITE-ProRule annotationAdd BLAST64
Domaini246 – 280WW 1PROSITE-ProRule annotationAdd BLAST35
Domaini299 – 333WW 2PROSITE-ProRule annotationAdd BLAST35
Domaini414 – 447WW 3PROSITE-ProRule annotationAdd BLAST34
Domaini477 – 593PHPROSITE-ProRule annotationAdd BLAST117
Domaini677 – 866Rho-GAPPROSITE-ProRule annotationAdd BLAST190

Keywords - Domaini

Repeat, SH3 domain

Phylogenomic databases

eggNOGiKOG1450 Eukaryota
KOG4269 Eukaryota
ENOG410ZP6T LUCA
GeneTreeiENSGT00930000150818
HOGENOMiHOG000294167
HOVERGENiHBG005328
InParanoidiA2AB59
KOiK20636
OMAiKGYIRDQ
OrthoDBiEOG091G164H
PhylomeDBiA2AB59
TreeFamiTF329345

Family and domain databases

CDDicd00201 WW, 2 hits
Gene3Di1.10.555.10, 1 hit
2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR011047 Quinoprotein_ADH-like_supfam
IPR008936 Rho_GTPase_activation_prot
IPR000198 RhoGAP_dom
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
IPR001202 WW_dom
IPR036020 WW_dom_sf
PfamiView protein in Pfam
PF00169 PH, 1 hit
PF00620 RhoGAP, 1 hit
PF00397 WW, 2 hits
SMARTiView protein in SMART
SM00233 PH, 1 hit
SM00324 RhoGAP, 1 hit
SM00456 WW, 3 hits
SUPFAMiSSF48350 SSF48350, 1 hit
SSF50044 SSF50044, 1 hit
SSF50998 SSF50998, 1 hit
SSF51045 SSF51045, 1 hit
PROSITEiView protein in PROSITE
PS50003 PH_DOMAIN, 1 hit
PS50238 RHOGAP, 1 hit
PS50002 SH3, 1 hit
PS50020 WW_DOMAIN_2, 3 hits

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: A2AB59-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAADVEGDVY VLVEHPFEYT GKDGRRIAIQ PNERYRLLRR STEHWWHVRR
60 70 80 90 100
EPGGRPFYLP AQYVRELPAL GDPAPAPQPS VPQQRPAVPE PLAYDYRFVS
110 120 130 140 150
TPVGADGSSA EPRGRASSLC GPARQRTGGQ RNSLAPGGPA CLYVRPAAPV
160 170 180 190 200
RPAQSLDDLA RGGTAPPAGL LGSAGHFKAS SVAGSWVCPR PLAPSDSENV
210 220 230 240 250
YEAIPDLRCP PRAESPKQVD DPPEPVYANV ERQPRATSPR SAAAPPRLSP
260 270 280 290 300
VWETHTDTGT GRPYYYNPDT GVTTWESPFE TPEGTTSPAT SRASVGSGES
310 320 330 340 350
LETEWGQYWD EESRRVFFYN PLTGETAWED ETEELEEDHQ EQLEMQPSLS
360 370 380 390 400
PRSPGQQRPP TPETDYPELL ASYPEEDYSP VGSFSDPGPA SPLVAPPGWS
410 420 430 440 450
CQITPDKQML YTNQFTQEQW VRLEDQHGKP YFYNPEDSSV QWELPQVPIP
460 470 480 490 500
APRSVRKSSQ DSDTPAQASP PEEKIKTLDK AGVLHRTKTV DKGKRLRKKH
510 520 530 540 550
WSTSWTVLEG GVLTFFKDSK TSAAGGLRQP SKLSTPEYTV ELKGASLSWA
560 570 580 590 600
PKDKSSKKNV LELRSRDGSE YLIQHDSEAI ISTWHKAIAE GISELSADLL
610 620 630 640 650
QGEEGEPSSA DFGSSERLGS WREEDVRQNA ASPSLSPGGL ESDLSRVRHK
660 670 680 690 700
LRKFLQRRPT LQSLRDKGYI KDQVFGCALA QLCERERSPV PRFVQQCIRT
710 720 730 740 750
VEARGLDIDG LYRISGNLAT IQKLRYKVDH DERLDLDDGR WEDVHVITGA
760 770 780 790 800
LKLFFRELPE PLFPFSHFHQ FIAAIKLQDP AQRSRCVRDL VRTLPAPNQD
810 820 830 840 850
TLRLLIQHLC RVIEHGEQNR MTVQNVAIVF GPTLLRPEME EASMPMTMVF
860
QNQVVELILH QCADIFPPH
Length:869
Mass (Da):97,049
Last modified:February 20, 2007 - v1
Checksum:iF53EDBAFE1B2A6E7
GO
Isoform 2 (identifier: A2AB59-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-217: MAADVEGDVY...RCPPRAESPK → MVDMISKLVRRQSRALRA

Note: No experimental confirmation available.Combined sources
Show »
Length:670
Mass (Da):75,802
Checksum:iEC550B4E2ADCCE9B
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A2AKN5A2AKN5_MOUSE
Rho GTPase-activating protein 27
Arhgap27 5730442P18Rik, Sh3d20, mCG_140725
243Annotation score:
A2AB70A2AB70_MOUSE
Rho GTPase-activating protein 27
Arhgap27
123Annotation score:
E9Q6V8E9Q6V8_MOUSE
Rho GTPase-activating protein 27
Arhgap27
91Annotation score:

Sequence cautioni

The sequence AAH96671 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0310571 – 217MAADV…AESPK → MVDMISKLVRRQSRALRA in isoform 2. 1 PublicationAdd BLAST217

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK046763 mRNA Translation: BAE20660.1
AL772325, AL662804 Genomic DNA Translation: CAM16599.1
AL662804, AL772325 Genomic DNA Translation: CAM21566.1
BC096671 mRNA Translation: AAH96671.1 Different initiation.
CCDSiCCDS25517.1 [A2AB59-2]
CCDS56815.1 [A2AB59-1]
RefSeqiNP_001192165.1, NM_001205236.1 [A2AB59-1]
NP_598476.2, NM_133715.5 [A2AB59-2]
UniGeneiMm.259113
Mm.397197

Genome annotation databases

EnsembliENSMUST00000041385; ENSMUSP00000039427; ENSMUSG00000034255 [A2AB59-2]
ENSMUST00000107024; ENSMUSP00000102639; ENSMUSG00000034255 [A2AB59-1]
GeneIDi544817
KEGGimmu:544817
UCSCiuc007luc.2 mouse [A2AB59-1]
uc007lud.2 mouse [A2AB59-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK046763 mRNA Translation: BAE20660.1
AL772325, AL662804 Genomic DNA Translation: CAM16599.1
AL662804, AL772325 Genomic DNA Translation: CAM21566.1
BC096671 mRNA Translation: AAH96671.1 Different initiation.
CCDSiCCDS25517.1 [A2AB59-2]
CCDS56815.1 [A2AB59-1]
RefSeqiNP_001192165.1, NM_001205236.1 [A2AB59-1]
NP_598476.2, NM_133715.5 [A2AB59-2]
UniGeneiMm.259113
Mm.397197

3D structure databases

ProteinModelPortaliA2AB59
SMRiA2AB59
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000102639

PTM databases

iPTMnetiA2AB59
PhosphoSitePlusiA2AB59

Proteomic databases

EPDiA2AB59
MaxQBiA2AB59
PaxDbiA2AB59
PeptideAtlasiA2AB59
PRIDEiA2AB59

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000041385; ENSMUSP00000039427; ENSMUSG00000034255 [A2AB59-2]
ENSMUST00000107024; ENSMUSP00000102639; ENSMUSG00000034255 [A2AB59-1]
GeneIDi544817
KEGGimmu:544817
UCSCiuc007luc.2 mouse [A2AB59-1]
uc007lud.2 mouse [A2AB59-2]

Organism-specific databases

CTDi201176
MGIiMGI:1916903 Arhgap27

Phylogenomic databases

eggNOGiKOG1450 Eukaryota
KOG4269 Eukaryota
ENOG410ZP6T LUCA
GeneTreeiENSGT00930000150818
HOGENOMiHOG000294167
HOVERGENiHBG005328
InParanoidiA2AB59
KOiK20636
OMAiKGYIRDQ
OrthoDBiEOG091G164H
PhylomeDBiA2AB59
TreeFamiTF329345

Enzyme and pathway databases

ReactomeiR-MMU-194840 Rho GTPase cycle

Miscellaneous databases

ChiTaRSiArhgap27 mouse
PROiPR:A2AB59
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000034255 Expressed in 190 organ(s), highest expression level in pyloric antrum
CleanExiMM_ARHGAP27
ExpressionAtlasiA2AB59 baseline and differential
GenevisibleiA2AB59 MM

Family and domain databases

CDDicd00201 WW, 2 hits
Gene3Di1.10.555.10, 1 hit
2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR011047 Quinoprotein_ADH-like_supfam
IPR008936 Rho_GTPase_activation_prot
IPR000198 RhoGAP_dom
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
IPR001202 WW_dom
IPR036020 WW_dom_sf
PfamiView protein in Pfam
PF00169 PH, 1 hit
PF00620 RhoGAP, 1 hit
PF00397 WW, 2 hits
SMARTiView protein in SMART
SM00233 PH, 1 hit
SM00324 RhoGAP, 1 hit
SM00456 WW, 3 hits
SUPFAMiSSF48350 SSF48350, 1 hit
SSF50044 SSF50044, 1 hit
SSF50998 SSF50998, 1 hit
SSF51045 SSF51045, 1 hit
PROSITEiView protein in PROSITE
PS50003 PH_DOMAIN, 1 hit
PS50238 RHOGAP, 1 hit
PS50002 SH3, 1 hit
PS50020 WW_DOMAIN_2, 3 hits
ProtoNetiSearch...

Entry informationi

Entry nameiRHG27_MOUSE
AccessioniPrimary (citable) accession number: A2AB59
Secondary accession number(s): Q3V366, Q4V9V5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: February 20, 2007
Last modified: November 7, 2018
This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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