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Entry version 101 (08 May 2019)
Sequence version 1 (05 Feb 2008)
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Protein

Histone-lysine N-methyltransferase PRDM16

Gene

Prdm16

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Binds DNA and functions as a transcriptional regulator (PubMed:18483224). Displays histone methyltransferase activity and monomethylates 'Lys-9' of histone H3 (H3K9me1) in vitro (PubMed:22939622). Probably catalyzes the monomethylation of free histone H3 in the cytoplasm which is then transported to the nucleus and incorporated into nucleosomes where SUV39H methyltransferases use it as a substrate to catalyze histone H3 'Lys-9' trimethylation (PubMed:22939622). Likely to be one of the primary histone methyltransferases along with MECOM/PRDM3 that direct cytoplasmic H3K9me1 methylation (PubMed:22939622). Functions in the differentiation of brown adipose tissue (BAT) which is specialized in dissipating chemical energy in the form of heat in response to cold or excess feeding while white adipose tissue (WAT) is specialized in the storage of excess energy and the control of systemic metabolism (PubMed:17618855, PubMed:18483224). Together with CEBPB, regulates the differentiation of myoblastic precursors into brown adipose cells (PubMed:18719582, PubMed:19641492). Functions as a repressor of TGF-beta signaling.5 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Function’ section specifies the position(s) and type(s) of zinc fingers within the protein.<p><a href='/help/zn_fing' target='_top'>More...</a></p>Zinc fingeri230 – 255C2H2-type 1; degeneratePROSITE-ProRule annotationAdd BLAST26
Zinc fingeri282 – 304C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri310 – 332C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri338 – 361C2H2-type 4PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri367 – 389C2H2-type 5PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri395 – 417C2H2-type 6PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri424 – 446C2H2-type 7; atypicalPROSITE-ProRule annotationAdd BLAST23
Zinc fingeri951 – 973C2H2-type 8PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri979 – 1002C2H2-type 9PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri1008 – 1030C2H2-type 10PROSITE-ProRule annotationAdd BLAST23

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionActivator, DNA-binding, Methyltransferase, Repressor, Transferase
Biological processDifferentiation, Transcription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

Reactome - a knowledgebase of biological pathways and processes

More...
Reactomei
R-MMU-3214841 PKMTs methylate histone lysines

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Histone-lysine N-methyltransferase PRDM16Curated (EC:2.1.1.431 Publication)
Alternative name(s):
PR domain zinc finger protein 16Curated
PR domain-containing protein 16Curated
Transcription factor MEL1Curated
Short name:
MDS1/EVI1-like gene 11 Publication
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:Prdm16Imported
Synonyms:Kiaa1675Curated, Mel11 Publication
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiMus musculus (Mouse)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri10090 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000589 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 4

Organism-specific databases

Mouse genome database (MGD) from Mouse Genome Informatics (MGI)

More...
MGIi
MGI:1917923 Prdm16

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the ‘Pathology and Biotech’ section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

Mice die at birth but embryos display altered brown adipose tissue differentiation.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi805 – 806DL → AS: Loss of interaction with CTBP1 and CTBP2 and loss of repression of WAT-specific genes. 1 Publication2
Mutagenesisi996R → Q: Loss of DNA-binding activity but no effect on PRDM16-mediated BAT gene transcription activation. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00003843771 – 1275Histone-lysine N-methyltransferase PRDM16Add BLAST1275

Proteomic databases

jPOST - Japan Proteome Standard Repository/Database

More...
jPOSTi
A2A935

MaxQB - The MaxQuant DataBase

More...
MaxQBi
A2A935

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
A2A935

PRoteomics IDEntifications database

More...
PRIDEi
A2A935

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
A2A935

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...
PhosphoSitePlusi
A2A935

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the ‘Expression’ section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified ‘at protein level’. <br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Enriched in BAT compared to WAT. Detected in heart, lung, kidney and brain. Expressed in nuclei of cardiomyocytes.2 Publications

<p>This subsection of the ‘Expression’ section provides information on the expression of the gene product at various stages of a cell, tissue or organism development. By default, the information is derived from experiments at the mRNA level, unless specified ‘at the protein level’.<p><a href='/help/developmental_stage' target='_top'>More...</a></p>Developmental stagei

Expressed at 12.5 dpc, 13.5 dpc and 14.5 dpc. Expressed in orofacial tissues, heart, liver, brain and limb bud. At 13.5 dpc, expressed throughout the ventricular myocardium, including endocardium and epicardium.2 Publications

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...
Bgeei
ENSMUSG00000039410 Expressed in 245 organ(s), highest expression level in pes

ExpressionAtlas, Differential and Baseline Expression

More...
ExpressionAtlasi
A2A935 baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...
Genevisiblei
A2A935 MM

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Interacts with CEBPA, CEBPB and CEBPD; the interaction is direct (PubMed:19641492). Interacts with PPARG and PPARA; controls brown adipocytes (PubMed:18719582). Interacts with CTBP1 and CTBP2; represses the expression of WAT-specific genes (PubMed:18483224). Interacts with PPARGC1A and PPARGC1B; interaction with PPARGC1A or PPARGC1B activates the transcription of BAT-specific gene (PubMed:18483224, PubMed:17618855). Interacts with HDAC1, SKI and SMAD2; the interaction with SKI promotes the recruitment of SMAD3-HDAC1 complex on the promoter of TGF-beta target genes (Probable). Interacts with ZNF516; the interaction is direct and may play a role in the transcription of brown adipose tissue-specific gene (PubMed:25578880).1 Publication5 Publications

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction_section%27">Interaction</a> section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="http://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated on a monthly basis. Each binary interaction is displayed on a separate line.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

WithEntry#Exp.IntActNotes
Ehmt1Q5DW34-12EBI-16080455,EBI-16080518

GO - Molecular functioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...
BioGridi
214194, 5 interactors

Database of interacting proteins

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DIPi
DIP-60593N

Protein interaction database and analysis system

More...
IntActi
A2A935, 949 interactors

STRING: functional protein association networks

More...
STRINGi
10090.ENSMUSP00000030902

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
A2A935

Database of comparative protein structure models

More...
ModBasei
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini82 – 211SETPROSITE-ProRule annotationAdd BLAST130

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni680 – 1038Interaction with CTBP1, CTBP2 and ZNF5161 PublicationAdd BLAST359
Regioni740 – 1275Mediates interaction with SKI and regulation of TGF-beta signalingBy similarityAdd BLAST536

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes the position of regions of compositional bias within the protein and the particular amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi460 – 558Pro-richAdd BLAST99

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the PRDM16 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri230 – 255C2H2-type 1; degeneratePROSITE-ProRule annotationAdd BLAST26
Zinc fingeri282 – 304C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri310 – 332C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri338 – 361C2H2-type 4PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri367 – 389C2H2-type 5PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri395 – 417C2H2-type 6PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri424 – 446C2H2-type 7; atypicalPROSITE-ProRule annotationAdd BLAST23
Zinc fingeri951 – 973C2H2-type 8PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri979 – 1002C2H2-type 9PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri1008 – 1030C2H2-type 10PROSITE-ProRule annotationAdd BLAST23

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
KOG1721 Eukaryota
COG5048 LUCA

Ensembl GeneTree

More...
GeneTreei
ENSGT00940000160951

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
HOG000231144

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
A2A935

KEGG Orthology (KO)

More...
KOi
K22410

Identification of Orthologs from Complete Genome Data

More...
OMAi
GMQEKKM

Database of Orthologous Groups

More...
OrthoDBi
1318335at2759

TreeFam database of animal gene trees

More...
TreeFami
TF315309

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR001214 SET_dom
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00096 zf-C2H2, 9 hits

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00317 SET, 1 hit
SM00355 ZnF_C2H2, 10 hits

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF57667 SSF57667, 5 hits

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS50280 SET, 1 hit
PS00028 ZINC_FINGER_C2H2_1, 8 hits
PS50157 ZINC_FINGER_C2H2_2, 10 hits

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (3+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 3 <p>This subsection of the ‘Sequence’ section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: A2A935-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MRSKARARKL AKSDGDVVNN MYEPDPDLLA GQSAEEETED GILSPIPMGP
60 70 80 90 100
PSPFPTSEDF TPKEGSPYEA PVYIPEDIPI PPDFELRESS IPGAGLGIWA
110 120 130 140 150
KRKMEIGERF GPYVVTPRAA LKEADFGWEM LTDTEVSSQE SCIKKQISED
160 170 180 190 200
LGSEKFCVDA NQAGSGSWLK YIRVACSCDD QNLAMCQINE QIYYKVIKDI
210 220 230 240 250
EPGEELLVHV KEGAYSLGVM APSLDEDPTF RCDECDELFQ CRLDLRRHKK
260 270 280 290 300
YACSSAGAQL YEGLGEELKP EGLGVGSDGQ AHECKDCERM FPNKYSLEQH
310 320 330 340 350
MIVHTEEREY KCDQCPKAFN WKSNLIRHQM SHDSGKRFEC ENCVKVFTDP
360 370 380 390 400
SNLQRHIRSQ HVGARAHACP DCGKTFATSS GLKQHKHIHS TVKPFICEVC
410 420 430 440 450
HKSYTQFSNL CRHKRMHADC RTQIKCKDCG QMFSTTSSLN KHRRFCEGKN
460 470 480 490 500
HYTPGSIFTP GLPLTPSPMM DKTKPSPTLN HGGLGFSEYF PSRPHPGSLP
510 520 530 540 550
FSAAPPAFPA LTPGFPGIFP PSLYPRPPLL PPTPLLKSPL NHAQDAKLPS
560 570 580 590 600
PLGNPALPLV SAVSNSSQGA TAATGSEEKF DGRLEDAYAE KVKNRSPDMS
610 620 630 640 650
DGSDFEDINT TTGTDLDTTT GTGSDLDSDL DSDRDKGKDK GKPVESKPEF
660 670 680 690 700
GGASVPPGAM NSVAEVPAFY SQHSFFPPPE EQLLTASGAA GDSIKAIASI
710 720 730 740 750
AEKYFGPGFM SMQEKKLGSL PYHSVFPFQF LPNFPHSLYP FTDRALAHNL
760 770 780 790 800
LVKAEPKSPR DALKVGGPSA ECPFDLTTKP KEAKPALLAP KVPLIPSSGE
810 820 830 840 850
EQPLDLSIGS RARASQNGGG REPRKNHVYG ERKPGVSEGL PKVCPAQLPQ
860 870 880 890 900
QPSLHYAKPS PFFMDPIYRV EKRKVADPVG VLKEKYLRPS PLLFHPQMSA
910 920 930 940 950
IETMTEKLES FAAMKADSGS SLQPLPHHPF NFRSPPPTLS DPILRKGKER
960 970 980 990 1000
YTCRYCGKIF PRSANLTRHL RTHTGEQPYR CKYCDRSFSI SSNLQRHVRN
1010 1020 1030 1040 1050
IHNKEKPFKC HLCNRCFGQQ TNLDRHLKKH EHEGAPVSQH SGVLTNHLGT
1060 1070 1080 1090 1100
SASSPTSESD NHALLDEKED SYFSEIRNFI ANSEMNQAST RMDKRPEIQD
1110 1120 1130 1140 1150
LDSNPPCPGS ASAKPEDVEE EEEEELEEED DDSLAGKSQE DTVSPTPEPQ
1160 1170 1180 1190 1200
GVYEDEEDEE PPSLTMGFDH TRRCVEERGG GLLALEPTPT FGKGLDLRRA
1210 1220 1230 1240 1250
AEEAFEVKDV LNSTLDSEVL KQTLYRQAKN QAYAMMLSLS EDTPLHAPSQ
1260 1270
SSLDAWLNIT GPSSESGAFN PINHL
Length:1,275
Mass (Da):140,858
Last modified:February 5, 2008 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i2F1F6B6C3EBFEC10
GO
Isoform 2 (identifier: A2A935-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     129-129: E → EQ
     868-868: Y → YS
     1174-1176: CVE → HMQ
     1177-1275: Missing.

Note: No experimental confirmation available.
Show »
Length:1,178
Mass (Da):130,427
Checksum:i9B050B76A7B9E29F
GO
Isoform 3 (identifier: A2A935-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     868-868: Y → YS

Note: No experimental confirmation available.
Show »
Length:1,276
Mass (Da):140,945
Checksum:i8A716E6E065ADEA4
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A2A933A2A933_MOUSE
Histone-lysine N-methyltransferase ...
Prdm16
1,276Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
A2A934A2A934_MOUSE
Histone-lysine N-methyltransferase ...
Prdm16
1,177Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
A2A931A2A931_MOUSE
Histone-lysine N-methyltransferase ...
Prdm16
1,274Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
A2A930A2A930_MOUSE
Histone-lysine N-methyltransferase ...
Prdm16
1,257Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
A2A929A2A929_MOUSE
Histone-lysine N-methyltransferase ...
Prdm16
214Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti8R → K in BAC79382 (PubMed:12816872).Curated1
Sequence conflicti510A → T in BAC79382 (PubMed:12816872).Curated1
Sequence conflicti706G → D in BAC79382 (PubMed:12816872).Curated1
Sequence conflicti732P → L in BAC79382 (PubMed:12816872).Curated1
Sequence conflicti760R → Q in BAC79382 (PubMed:12816872).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_038066129E → EQ in isoform 2. 1 Publication1
Alternative sequenceiVSP_038067868Y → YS in isoform 2 and isoform 3. 2 Publications1
Alternative sequenceiVSP_0380681174 – 1176CVE → HMQ in isoform 2. 1 Publication3
Alternative sequenceiVSP_0380691177 – 1275Missing in isoform 2. 1 PublicationAdd BLAST99

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AB078338 mRNA Translation: BAC79382.1
AL611950 Genomic DNA No translation available.
AL627123 Genomic DNA No translation available.
AL627127 Genomic DNA No translation available.
AL627226 Genomic DNA No translation available.
BC059838 mRNA Translation: AAH59838.1
AK173230 mRNA Translation: BAD32508.1

The Consensus CDS (CCDS) project

More...
CCDSi
CCDS38989.1 [A2A935-1]
CCDS71532.1 [A2A935-2]

NCBI Reference Sequences

More...
RefSeqi
NP_001277955.1, NM_001291026.1
NP_001277958.1, NM_001291029.1 [A2A935-2]
NP_081780.3, NM_027504.3 [A2A935-1]
XP_006539236.1, XM_006539173.3 [A2A935-3]

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...
Ensembli
ENSMUST00000030902; ENSMUSP00000030902; ENSMUSG00000039410 [A2A935-1]
ENSMUST00000070313; ENSMUSP00000064546; ENSMUSG00000039410 [A2A935-2]

Database of genes from NCBI RefSeq genomes

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GeneIDi
70673

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
mmu:70673

UCSC genome browser

More...
UCSCi
uc008wbu.1 mouse [A2A935-1]
uc008wbx.1 mouse [A2A935-2]

Keywords - Coding sequence diversityi

Alternative splicing

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB078338 mRNA Translation: BAC79382.1
AL611950 Genomic DNA No translation available.
AL627123 Genomic DNA No translation available.
AL627127 Genomic DNA No translation available.
AL627226 Genomic DNA No translation available.
BC059838 mRNA Translation: AAH59838.1
AK173230 mRNA Translation: BAD32508.1
CCDSiCCDS38989.1 [A2A935-1]
CCDS71532.1 [A2A935-2]
RefSeqiNP_001277955.1, NM_001291026.1
NP_001277958.1, NM_001291029.1 [A2A935-2]
NP_081780.3, NM_027504.3 [A2A935-1]
XP_006539236.1, XM_006539173.3 [A2A935-3]

3D structure databases

SMRiA2A935
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi214194, 5 interactors
DIPiDIP-60593N
IntActiA2A935, 949 interactors
STRINGi10090.ENSMUSP00000030902

PTM databases

iPTMnetiA2A935
PhosphoSitePlusiA2A935

Proteomic databases

jPOSTiA2A935
MaxQBiA2A935
PaxDbiA2A935
PRIDEiA2A935

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030902; ENSMUSP00000030902; ENSMUSG00000039410 [A2A935-1]
ENSMUST00000070313; ENSMUSP00000064546; ENSMUSG00000039410 [A2A935-2]
GeneIDi70673
KEGGimmu:70673
UCSCiuc008wbu.1 mouse [A2A935-1]
uc008wbx.1 mouse [A2A935-2]

Organism-specific databases

Comparative Toxicogenomics Database

More...
CTDi
63976
MGIiMGI:1917923 Prdm16

Rodent Unidentified Gene-Encoded large proteins database

More...
Rougei
Search...

Phylogenomic databases

eggNOGiKOG1721 Eukaryota
COG5048 LUCA
GeneTreeiENSGT00940000160951
HOGENOMiHOG000231144
InParanoidiA2A935
KOiK22410
OMAiGMQEKKM
OrthoDBi1318335at2759
TreeFamiTF315309

Enzyme and pathway databases

ReactomeiR-MMU-3214841 PKMTs methylate histone lysines

Miscellaneous databases

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...
ChiTaRSi
Prdm16 mouse

Protein Ontology

More...
PROi
PR:A2A935

The Stanford Online Universal Resource for Clones and ESTs

More...
SOURCEi
Search...

Gene expression databases

BgeeiENSMUSG00000039410 Expressed in 245 organ(s), highest expression level in pes
ExpressionAtlasiA2A935 baseline and differential
GenevisibleiA2A935 MM

Family and domain databases

InterProiView protein in InterPro
IPR001214 SET_dom
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PfamiView protein in Pfam
PF00096 zf-C2H2, 9 hits
SMARTiView protein in SMART
SM00317 SET, 1 hit
SM00355 ZnF_C2H2, 10 hits
SUPFAMiSSF57667 SSF57667, 5 hits
PROSITEiView protein in PROSITE
PS50280 SET, 1 hit
PS00028 ZINC_FINGER_C2H2_1, 8 hits
PS50157 ZINC_FINGER_C2H2_2, 10 hits

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiPRD16_MOUSE
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: A2A935
Secondary accession number(s): Q69ZD6, Q6PB79, Q7TPF4
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: February 5, 2008
Last modified: May 8, 2019
This is version 101 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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