UniProtKB - A2A8U2 (TM201_MOUSE)
Protein
Transmembrane protein 201
Gene
Tmem201
Organism
Mus musculus (Mouse)
Status
Functioni
Involved in nuclear movement during fibroblast polarization and migration (PubMed:22349700). May recruit Ran GTPase to the nuclear periphery (By similarity).By similarity1 Publication
Isoform 2: May define a distinct membrane domain in the vicinity of the mitotic spindle. Involved in the organization of the nuclear envelope implicating EMD, SUN1 and A-type lamina.By similarity
Isoform 3: Proposed to be involved in actin-dependent nuclear movement; via SUN2 associates with transmembrane actin-associated nuclear (TAN) lines which are bound to F-actin cables and couple the nucleus to retrograde actin flow.1 Publication
GO - Molecular functioni
- actin filament binding Source: MGI
- lamin binding Source: MGI
GO - Biological processi
- centrosome localization Source: MGI
- fibroblast migration Source: MGI
- nuclear envelope organization Source: MGI
- nuclear migration Source: MGI
- nuclear migration along microtubule Source: GO_Central
- protein localization to nuclear envelope Source: MGI
Names & Taxonomyi
Protein namesi | Recommended name: Transmembrane protein 201Alternative name(s): Spindle-associated membrane protein 1 |
Gene namesi | Name:Tmem201 Synonyms:D4Ertd429e, Net5, Samp1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1196277 Tmem201 |
Subcellular locationi
Isoform 2 :
Nucleus
- Nucleus inner membrane By similarity; Multi-pass membrane protein By similarity
Note: The C-terminal of isoform 2 is located on the nucleoplasmic side. During interphase, isoform 2 is distributed in the inner nuclear membrane and during mitosis, it is found in the ER but it also localizes to the polar regions of the mitotic spindle (By similarity).By similarity
Nucleus
- integral component of nuclear inner membrane Source: MGI
- nuclear envelope Source: MGI
- nuclear membrane Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 214 | NuclearBy similarityAdd BLAST | 214 | |
Transmembranei | 215 – 235 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 236 – 297 | Perinuclear spaceCuratedAdd BLAST | 62 | |
Transmembranei | 298 – 318 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 319 – 322 | NuclearCurated | 4 | |
Transmembranei | 323 – 343 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 344 – 356 | Perinuclear spaceCuratedAdd BLAST | 13 | |
Transmembranei | 357 – 374 | HelicalSequence analysisAdd BLAST | 18 | |
Topological domaini | 375 – 642 | NuclearBy similarityCuratedAdd BLAST | 268 | |
Transmembranei | 643 – 663 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 664 | Perinuclear spaceCurated | 1 |
Keywords - Cellular componenti
Membrane, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000317199 | 1 – 664 | Transmembrane protein 201Add BLAST | 664 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 441 | PhosphoserineBy similarity | 1 | |
Modified residuei | 444 | PhosphoserineBy similarity | 1 | |
Modified residuei | 450 | PhosphoserineBy similarity | 1 | |
Modified residuei | 454 | PhosphoserineBy similarity | 1 | |
Modified residuei | 466 | PhosphoserineBy similarity | 1 | |
Modified residuei | 477 | PhosphoserineBy similarity | 1 | |
Modified residuei | 480 | PhosphoserineBy similarity | 1 | |
Modified residuei | 529 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
jPOSTi | A2A8U2 |
MaxQBi | A2A8U2 |
PaxDbi | A2A8U2 |
PeptideAtlasi | A2A8U2 |
PRIDEi | A2A8U2 |
PTM databases
iPTMneti | A2A8U2 |
PhosphoSitePlusi | A2A8U2 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000044700 Expressed in 233 organ(s), highest expression level in embryo |
Genevisiblei | A2A8U2 MM |
Interactioni
Subunit structurei
Isoform 2 interacts with EMD (By similarity). Isoform 3 interacts with SUN2 and LMNA (PubMed:22349700). May bind to Ran GTPase; has a greater affinity for Ran-GTP over Ran-GDP (By similarity).By similarity1 Publication
Binary interactionsi
With | Entry | #Exp. | IntAct | Notes |
---|---|---|---|---|
Sun2 | Q8BJS4 | 3 | EBI-12591474,EBI-646914 |
GO - Molecular functioni
- actin filament binding Source: MGI
- lamin binding Source: MGI
Protein-protein interaction databases
BioGridi | 231056, 1 interactor |
IntActi | A2A8U2, 4 interactors |
MINTi | A2A8U2 |
STRINGi | 10090.ENSMUSP00000050481 |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 390 – 520 | Ser-richAdd BLAST | 131 |
Sequence similaritiesi
Belongs to the TMEM201 family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG4623 Eukaryota ENOG41123RR LUCA |
GeneTreei | ENSGT00390000002713 |
HOGENOMi | HOG000154619 |
HOVERGENi | HBG057857 |
InParanoidi | A2A8U2 |
OMAi | QSHQMGV |
OrthoDBi | 532133at2759 |
PhylomeDBi | A2A8U2 |
TreeFami | TF106107 |
Family and domain databases
InterProi | View protein in InterPro IPR018617 Ima1_N IPR040041 TMEM201 IPR018861 TMEM201_C |
PANTHERi | PTHR28646 PTHR28646, 1 hit |
Pfami | View protein in Pfam PF10476 DUF2448, 1 hit PF09779 Ima1_N, 1 hit |
s (3)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: A2A8U2-1) [UniParc]FASTAAdd to basket
Also known as: Samp1c
This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
10 20 30 40 50
MEGVSALLAS CPTAGLAGGL GVTACAAAGV VLYRIARRVK PTHTMVNCWF
60 70 80 90 100
CNHDTLVPYG NRNCWDCPHC EQYNGFQENG DYNKPIPAQY MEHLNHVVSS
110 120 130 140 150
VPSPRDPAQP QQWVSSQVLL CRRCSHHQTT KIKQLAAFTP REEGRYDEEI
160 170 180 190 200
EVYRHHLEQM YKLCRPCQAA VEYYIKHQNR QLRALLLSHQ FRRREADQAH
210 220 230 240 250
GQSFSSSAVK APFQVILLRA LAFLACAFLL FTTLYGPSEP FTPGAALPPA
260 270 280 290 300
LPPGGNSSAA SDNTTSQAEG WQQLLGLLPE HATEKLHEAW AFGQSHQTSI
310 320 330 340 350
VAVGLLTCLL AMLLAGRIRL RRIDAFSTCL WALLLGLHLA EHYLQAASPG
360 370 380 390 400
WLDTLKFSTT SLCCLVGFTA AVATRKSTGP RRFRPRRYFS GDSASLFPSS
410 420 430 440 450
PSLAVPYPSV TSSPASLFIP TPPGFLPLTK QQLFRSPRRV SPSSLPGRLS
460 470 480 490 500
RALSLGTIPP LTRTDSGYLF SGSRPPSRVS PAGEVSLSDY FSLLSSSFPA
510 520 530 540 550
SPLPSPAPSV ASSVASSSGS LRHRRPLISP ARLNLKGQKL LLFSSPGEAP
560 570 580 590 600
NTPSSSEEFS PPNGSLFIES PQLPQRNHTR DTKHTMEMRS MLARDSARSS
610 620 630 640 650
HSIKKEDESS QSSTCVVDTT TKGCSEETTP WKARVSPSLV RGLLAVSLAV
660
NALFTSAYLY QSLR
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 128 | Q → H in BAC37562 (PubMed:16141072).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_030917 | 388 – 392 | YFSGD → SEKQQ in isoform 2. Curated | 5 | |
Alternative sequenceiVSP_030918 | 393 – 664 | Missing in isoform 2. CuratedAdd BLAST | 272 | |
Alternative sequenceiVSP_030919 | 633 – 634 | AR → GL in isoform 3. 1 Publication | 2 | |
Alternative sequenceiVSP_030920 | 635 – 664 | Missing in isoform 3. 1 PublicationAdd BLAST | 30 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK079157 mRNA Translation: BAC37562.1 AK153631 mRNA Translation: BAE32126.1 AL626808 Genomic DNA No translation available. BC072645 mRNA Translation: AAH72645.1 |
CCDSi | CCDS18965.1 [A2A8U2-2] CCDS71523.1 [A2A8U2-1] |
RefSeqi | NP_001271199.1, NM_001284270.1 [A2A8U2-1] NP_001271202.1, NM_001284273.1 NP_808340.2, NM_177672.4 [A2A8U2-2] |
UniGenei | Mm.266704 |
Genome annotation databases
Ensembli | ENSMUST00000054459; ENSMUSP00000050481; ENSMUSG00000044700 [A2A8U2-3] ENSMUST00000103208; ENSMUSP00000099497; ENSMUSG00000044700 [A2A8U2-2] ENSMUST00000105687; ENSMUSP00000101312; ENSMUSG00000044700 [A2A8U2-1] |
GeneIDi | 230917 |
KEGGi | mmu:230917 |
UCSCi | uc008vwz.2 mouse [A2A8U2-1] uc008vxa.3 mouse [A2A8U2-3] uc008vxc.2 mouse [A2A8U2-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK079157 mRNA Translation: BAC37562.1 AK153631 mRNA Translation: BAE32126.1 AL626808 Genomic DNA No translation available. BC072645 mRNA Translation: AAH72645.1 |
CCDSi | CCDS18965.1 [A2A8U2-2] CCDS71523.1 [A2A8U2-1] |
RefSeqi | NP_001271199.1, NM_001284270.1 [A2A8U2-1] NP_001271202.1, NM_001284273.1 NP_808340.2, NM_177672.4 [A2A8U2-2] |
UniGenei | Mm.266704 |
3D structure databases
ModBasei | Search... |
MobiDBi | Search... |
Protein-protein interaction databases
BioGridi | 231056, 1 interactor |
IntActi | A2A8U2, 4 interactors |
MINTi | A2A8U2 |
STRINGi | 10090.ENSMUSP00000050481 |
PTM databases
iPTMneti | A2A8U2 |
PhosphoSitePlusi | A2A8U2 |
Proteomic databases
jPOSTi | A2A8U2 |
MaxQBi | A2A8U2 |
PaxDbi | A2A8U2 |
PeptideAtlasi | A2A8U2 |
PRIDEi | A2A8U2 |
Protocols and materials databases
Structural Biology Knowledgebase | Search... |
Genome annotation databases
Ensembli | ENSMUST00000054459; ENSMUSP00000050481; ENSMUSG00000044700 [A2A8U2-3] ENSMUST00000103208; ENSMUSP00000099497; ENSMUSG00000044700 [A2A8U2-2] ENSMUST00000105687; ENSMUSP00000101312; ENSMUSG00000044700 [A2A8U2-1] |
GeneIDi | 230917 |
KEGGi | mmu:230917 |
UCSCi | uc008vwz.2 mouse [A2A8U2-1] uc008vxa.3 mouse [A2A8U2-3] uc008vxc.2 mouse [A2A8U2-2] |
Organism-specific databases
CTDi | 199953 |
MGIi | MGI:1196277 Tmem201 |
Phylogenomic databases
eggNOGi | KOG4623 Eukaryota ENOG41123RR LUCA |
GeneTreei | ENSGT00390000002713 |
HOGENOMi | HOG000154619 |
HOVERGENi | HBG057857 |
InParanoidi | A2A8U2 |
OMAi | QSHQMGV |
OrthoDBi | 532133at2759 |
PhylomeDBi | A2A8U2 |
TreeFami | TF106107 |
Miscellaneous databases
ChiTaRSi | Tmem201 mouse |
PROi | PR:A2A8U2 |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000044700 Expressed in 233 organ(s), highest expression level in embryo |
Genevisiblei | A2A8U2 MM |
Family and domain databases
InterProi | View protein in InterPro IPR018617 Ima1_N IPR040041 TMEM201 IPR018861 TMEM201_C |
PANTHERi | PTHR28646 PTHR28646, 1 hit |
Pfami | View protein in Pfam PF10476 DUF2448, 1 hit PF09779 Ima1_N, 1 hit |
ProtoNeti | Search... |
Entry informationi
Entry namei | TM201_MOUSE | |
Accessioni | A2A8U2Primary (citable) accession number: A2A8U2 Secondary accession number(s): Q3U5F3, Q6GQS9, Q8BNY3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 5, 2008 |
Last sequence update: | February 20, 2007 | |
Last modified: | February 13, 2019 | |
This is version 87 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot