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Protein

Transmembrane protein 201

Gene

Tmem201

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in nuclear movement during fibroblast polarization and migration (PubMed:22349700). May recruit Ran GTPase to the nuclear periphery (By similarity).By similarity1 Publication
Isoform 2: May define a distinct membrane domain in the vicinity of the mitotic spindle. Involved in the organization of the nuclear envelope implicating EMD, SUN1 and A-type lamina.By similarity
Isoform 3: Proposed to be involved in actin-dependent nuclear movement; via SUN2 associates with transmembrane actin-associated nuclear (TAN) lines which are bound to F-actin cables and couple the nucleus to retrograde actin flow.1 Publication

GO - Molecular functioni

  • actin filament binding Source: MGI
  • lamin binding Source: MGI

GO - Biological processi

  • centrosome localization Source: MGI
  • fibroblast migration Source: MGI
  • nuclear envelope organization Source: MGI
  • nuclear migration Source: MGI
  • nuclear migration along microtubule Source: GO_Central
  • protein localization to nuclear envelope Source: MGI

Names & Taxonomyi

Protein namesi
Recommended name:
Transmembrane protein 201
Alternative name(s):
Spindle-associated membrane protein 1
Gene namesi
Name:Tmem201
Synonyms:D4Ertd429e, Net5, Samp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1196277 Tmem201

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 214NuclearBy similarityAdd BLAST214
Transmembranei215 – 235HelicalSequence analysisAdd BLAST21
Topological domaini236 – 297Perinuclear spaceCuratedAdd BLAST62
Transmembranei298 – 318HelicalSequence analysisAdd BLAST21
Topological domaini319 – 322NuclearCurated4
Transmembranei323 – 343HelicalSequence analysisAdd BLAST21
Topological domaini344 – 356Perinuclear spaceCuratedAdd BLAST13
Transmembranei357 – 374HelicalSequence analysisAdd BLAST18
Topological domaini375 – 642NuclearBy similarityCuratedAdd BLAST268
Transmembranei643 – 663HelicalSequence analysisAdd BLAST21
Topological domaini664Perinuclear spaceCurated1

Keywords - Cellular componenti

Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003171991 – 664Transmembrane protein 201Add BLAST664

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei441PhosphoserineBy similarity1
Modified residuei444PhosphoserineBy similarity1
Modified residuei450PhosphoserineBy similarity1
Modified residuei454PhosphoserineBy similarity1
Modified residuei466PhosphoserineBy similarity1
Modified residuei477PhosphoserineBy similarity1
Modified residuei480PhosphoserineBy similarity1
Modified residuei529PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiA2A8U2
PaxDbiA2A8U2
PeptideAtlasiA2A8U2
PRIDEiA2A8U2

PTM databases

iPTMnetiA2A8U2
PhosphoSitePlusiA2A8U2

Expressioni

Gene expression databases

BgeeiENSMUSG00000044700 Expressed in 233 organ(s), highest expression level in embryo
CleanExiMM_TMEM201
GenevisibleiA2A8U2 MM

Interactioni

Subunit structurei

Isoform 2 interacts with EMD (By similarity). Isoform 3 interacts with SUN2 and LMNA (PubMed:22349700). May bind to Ran GTPase; has a greater affinity for Ran-GTP over Ran-GDP (By similarity).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Sun2Q8BJS43EBI-12591474,EBI-646914

GO - Molecular functioni

Protein-protein interaction databases

BioGridi231056, 1 interactor
IntActiA2A8U2, 4 interactors
MINTiA2A8U2
STRINGi10090.ENSMUSP00000050481

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi390 – 520Ser-richAdd BLAST131

Sequence similaritiesi

Belongs to the TMEM201 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4623 Eukaryota
ENOG41123RR LUCA
GeneTreeiENSGT00390000002713
HOGENOMiHOG000154619
HOVERGENiHBG057857
InParanoidiA2A8U2
OMAiQSHQMGV
OrthoDBiEOG091G07TP
PhylomeDBiA2A8U2
TreeFamiTF106107

Family and domain databases

InterProiView protein in InterPro
IPR018617 Ima1_N
IPR040041 TMEM201
IPR018861 TMEM201_C
PANTHERiPTHR28646 PTHR28646, 1 hit
PfamiView protein in Pfam
PF10476 DUF2448, 1 hit
PF09779 Ima1_N, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: A2A8U2-1) [UniParc]FASTAAdd to basket
Also known as: Samp1c

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MEGVSALLAS CPTAGLAGGL GVTACAAAGV VLYRIARRVK PTHTMVNCWF
60 70 80 90 100
CNHDTLVPYG NRNCWDCPHC EQYNGFQENG DYNKPIPAQY MEHLNHVVSS
110 120 130 140 150
VPSPRDPAQP QQWVSSQVLL CRRCSHHQTT KIKQLAAFTP REEGRYDEEI
160 170 180 190 200
EVYRHHLEQM YKLCRPCQAA VEYYIKHQNR QLRALLLSHQ FRRREADQAH
210 220 230 240 250
GQSFSSSAVK APFQVILLRA LAFLACAFLL FTTLYGPSEP FTPGAALPPA
260 270 280 290 300
LPPGGNSSAA SDNTTSQAEG WQQLLGLLPE HATEKLHEAW AFGQSHQTSI
310 320 330 340 350
VAVGLLTCLL AMLLAGRIRL RRIDAFSTCL WALLLGLHLA EHYLQAASPG
360 370 380 390 400
WLDTLKFSTT SLCCLVGFTA AVATRKSTGP RRFRPRRYFS GDSASLFPSS
410 420 430 440 450
PSLAVPYPSV TSSPASLFIP TPPGFLPLTK QQLFRSPRRV SPSSLPGRLS
460 470 480 490 500
RALSLGTIPP LTRTDSGYLF SGSRPPSRVS PAGEVSLSDY FSLLSSSFPA
510 520 530 540 550
SPLPSPAPSV ASSVASSSGS LRHRRPLISP ARLNLKGQKL LLFSSPGEAP
560 570 580 590 600
NTPSSSEEFS PPNGSLFIES PQLPQRNHTR DTKHTMEMRS MLARDSARSS
610 620 630 640 650
HSIKKEDESS QSSTCVVDTT TKGCSEETTP WKARVSPSLV RGLLAVSLAV
660
NALFTSAYLY QSLR
Length:664
Mass (Da):72,500
Last modified:February 20, 2007 - v1
Checksum:iFF1207C8C15B33C2
GO
Isoform 2 (identifier: A2A8U2-2) [UniParc]FASTAAdd to basket
Also known as: Samp1, Samp1a

The sequence of this isoform differs from the canonical sequence as follows:
     388-392: YFSGD → SEKQQ
     393-664: Missing.

Show »
Length:392
Mass (Da):43,549
Checksum:i054F1EB65DE10C25
GO
Isoform 3 (identifier: A2A8U2-3) [UniParc]FASTAAdd to basket
Also known as: Samp1b

The sequence of this isoform differs from the canonical sequence as follows:
     633-634: AR → GL
     635-664: Missing.

Show »
Length:634
Mass (Da):69,251
Checksum:i61A5102FA5DD600D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti128Q → H in BAC37562 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_030917388 – 392YFSGD → SEKQQ in isoform 2. Curated5
Alternative sequenceiVSP_030918393 – 664Missing in isoform 2. CuratedAdd BLAST272
Alternative sequenceiVSP_030919633 – 634AR → GL in isoform 3. 1 Publication2
Alternative sequenceiVSP_030920635 – 664Missing in isoform 3. 1 PublicationAdd BLAST30

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK079157 mRNA Translation: BAC37562.1
AK153631 mRNA Translation: BAE32126.1
AL626808 Genomic DNA No translation available.
BC072645 mRNA Translation: AAH72645.1
CCDSiCCDS18965.1 [A2A8U2-2]
CCDS71523.1 [A2A8U2-1]
RefSeqiNP_001271199.1, NM_001284270.1 [A2A8U2-1]
NP_001271202.1, NM_001284273.1
NP_808340.2, NM_177672.4 [A2A8U2-2]
UniGeneiMm.266704

Genome annotation databases

EnsembliENSMUST00000054459; ENSMUSP00000050481; ENSMUSG00000044700 [A2A8U2-3]
ENSMUST00000103208; ENSMUSP00000099497; ENSMUSG00000044700 [A2A8U2-2]
ENSMUST00000105687; ENSMUSP00000101312; ENSMUSG00000044700 [A2A8U2-1]
GeneIDi230917
KEGGimmu:230917
UCSCiuc008vwz.2 mouse [A2A8U2-1]
uc008vxa.3 mouse [A2A8U2-3]
uc008vxc.2 mouse [A2A8U2-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK079157 mRNA Translation: BAC37562.1
AK153631 mRNA Translation: BAE32126.1
AL626808 Genomic DNA No translation available.
BC072645 mRNA Translation: AAH72645.1
CCDSiCCDS18965.1 [A2A8U2-2]
CCDS71523.1 [A2A8U2-1]
RefSeqiNP_001271199.1, NM_001284270.1 [A2A8U2-1]
NP_001271202.1, NM_001284273.1
NP_808340.2, NM_177672.4 [A2A8U2-2]
UniGeneiMm.266704

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi231056, 1 interactor
IntActiA2A8U2, 4 interactors
MINTiA2A8U2
STRINGi10090.ENSMUSP00000050481

PTM databases

iPTMnetiA2A8U2
PhosphoSitePlusiA2A8U2

Proteomic databases

MaxQBiA2A8U2
PaxDbiA2A8U2
PeptideAtlasiA2A8U2
PRIDEiA2A8U2

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000054459; ENSMUSP00000050481; ENSMUSG00000044700 [A2A8U2-3]
ENSMUST00000103208; ENSMUSP00000099497; ENSMUSG00000044700 [A2A8U2-2]
ENSMUST00000105687; ENSMUSP00000101312; ENSMUSG00000044700 [A2A8U2-1]
GeneIDi230917
KEGGimmu:230917
UCSCiuc008vwz.2 mouse [A2A8U2-1]
uc008vxa.3 mouse [A2A8U2-3]
uc008vxc.2 mouse [A2A8U2-2]

Organism-specific databases

CTDi199953
MGIiMGI:1196277 Tmem201

Phylogenomic databases

eggNOGiKOG4623 Eukaryota
ENOG41123RR LUCA
GeneTreeiENSGT00390000002713
HOGENOMiHOG000154619
HOVERGENiHBG057857
InParanoidiA2A8U2
OMAiQSHQMGV
OrthoDBiEOG091G07TP
PhylomeDBiA2A8U2
TreeFamiTF106107

Miscellaneous databases

ChiTaRSiTmem201 mouse
PROiPR:A2A8U2
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000044700 Expressed in 233 organ(s), highest expression level in embryo
CleanExiMM_TMEM201
GenevisibleiA2A8U2 MM

Family and domain databases

InterProiView protein in InterPro
IPR018617 Ima1_N
IPR040041 TMEM201
IPR018861 TMEM201_C
PANTHERiPTHR28646 PTHR28646, 1 hit
PfamiView protein in Pfam
PF10476 DUF2448, 1 hit
PF09779 Ima1_N, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTM201_MOUSE
AccessioniPrimary (citable) accession number: A2A8U2
Secondary accession number(s): Q3U5F3, Q6GQS9, Q8BNY3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: February 20, 2007
Last modified: November 7, 2018
This is version 85 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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