UniProtKB - A2A8L1 (CHD5_MOUSE)
Chromodomain-helicase-DNA-binding protein 5
Chd5
Functioni
Chromatin-remodeling protein that binds DNA through histones and regulates gene transcription. May specifically recognize and bind trimethylated 'Lys-27' (H3K27me3) and non-methylated 'Lys-4' of histone H3. Plays a role in the development of the nervous system by activating the expression of genes promoting neuron terminal differentiation. In parallel, it may also positively regulate the trimethylation of histone H3 at 'Lys-27' thereby specifically repressing genes that promote the differentiation into non-neuronal cell lineages. Tumor suppressor, it regulates the expression of genes involved in cell proliferation and differentiation. Downstream activated genes may include CDKN2A that positively regulates the p53/TP53 pathway, which in turn, prevents cell proliferation. In spermatogenesis, it probably regulates histone hyperacetylation and the replacement of histones by transition proteins in chromatin, a crucial step in the condensation of spermatid chromatin and the production of functional spermatozoa.
4 PublicationsCatalytic activityi
- EC:3.6.4.12
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 345 – 392 | PHD-type 1PROSITE-ProRule annotationAdd BLAST | 48 | |
Zinc fingeri | 418 – 465 | PHD-type 2PROSITE-ProRule annotationAdd BLAST | 48 | |
Nucleotide bindingi | 727 – 734 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- ATP hydrolysis activity Source: RHEA
- chromatin binding Source: MGI
- DNA binding Source: UniProtKB-KW
- DNA helicase activity Source: UniProtKB-EC
- H3K27me3 modified histone binding Source: UniProtKB
- histone binding Source: GO_Central
- histone deacetylase binding Source: UniProtKB
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- cerebral cortex neuron differentiation Source: UniProtKB
- histone H3-K27 trimethylation Source: UniProtKB
- histone H4 acetylation Source: UniProtKB
- negative regulation of cell population proliferation Source: UniProtKB
- negative regulation of transcription by RNA polymerase II Source: GO_Central
- positive regulation of signal transduction by p53 class mediator Source: UniProtKB
- regulation of cell differentiation Source: UniProtKB
- regulation of transcription by RNA polymerase II Source: MGI
- spermatogenesis, exchange of chromosomal proteins Source: UniProtKB
- transcription by RNA polymerase II Source: MGI
Keywordsi
Molecular function | Chromatin regulator, DNA-binding, Helicase, Hydrolase |
Biological process | Differentiation, Neurogenesis, Spermatogenesis, Transcription, Transcription regulation |
Ligand | ATP-binding, Metal-binding, Nucleotide-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Chromodomain-helicase-DNA-binding protein 5 (EC:3.6.4.12)Short name: CHD-5 Alternative name(s): ATP-dependent helicase CHD5 |
Gene namesi | Name:Chd5 Synonyms:Kiaa0444 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:3036258, Chd5 |
VEuPathDBi | HostDB:ENSMUSG00000005045 |
Subcellular locationi
Nucleus
- Nucleus 2 Publications
Note: Associates with heterochromatin.
Cytosol
- cytosol Source: MGI
Nucleus
- nuclear speck Source: MGI
- nucleoplasm Source: MGI
- nucleus Source: UniProtKB
- NuRD complex Source: UniProtKB
Other locations
- heterochromatin Source: UniProtKB
Keywords - Cellular componenti
NucleusPathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 346 | D → A: No effect on interaction with histone H3 unmethylated at 'Lys-4'. No significant effect on the ability to repress target genes expression. No effect on regulation of cell proliferation. 1 Publication | 1 | |
Mutagenesisi | 355 | G → A: Loss of the ability to negatively regulate cell proliferation. 1 Publication | 1 | |
Mutagenesisi | 361 | D → A: Loss of interaction with histone H3 unmethylated at 'Lys-4'. Loss of the ability to repress target genes expression. Loss of the ability to negatively regulate cell proliferation. 1 Publication | 1 | |
Mutagenesisi | 415 | D → A: Loss of interaction with histone H3 unmethylated at 'Lys-4'. Loss of the ability to negatively regulate cell proliferation. 1 Publication | 1 | |
Mutagenesisi | 432 | C → W: Loss of the ability to negatively regulate cell proliferation. 1 Publication | 1 | |
Mutagenesisi | 434 | D → A: Loss of interaction with histone H3 unmethylated at 'Lys-4'. Loss of the ability to repress target genes expression. Loss of the ability to negatively regulate cell proliferation. 1 Publication | 1 |
Keywords - Diseasei
Tumor suppressorPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000429326 | 1 – 1946 | Chromodomain-helicase-DNA-binding protein 5Add BLAST | 1946 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1392 | N5-methylglutamineBy similarity | 1 | |
Modified residuei | 1556 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Methylation, PhosphoproteinProteomic databases
jPOSTi | A2A8L1 |
MaxQBi | A2A8L1 |
PaxDbi | A2A8L1 |
PeptideAtlasi | A2A8L1 |
PRIDEi | A2A8L1 |
ProteomicsDBi | 281603 |
PTM databases
iPTMneti | A2A8L1 |
PhosphoSitePlusi | A2A8L1 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000005045, Expressed in primary visual cortex and 163 other tissues |
ExpressionAtlasi | A2A8L1, baseline and differential |
Genevisiblei | A2A8L1, MM |
Interactioni
Subunit structurei
May be part of a nucleosome remodeling and histone deacetylation, NuRD-like, complex composed at least of GATAD2B, HDAC1, HDAC2 and MTA3.
1 PublicationGO - Molecular functioni
- H3K27me3 modified histone binding Source: UniProtKB
- histone binding Source: GO_Central
- histone deacetylase binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 234678, 11 interactors |
IntActi | A2A8L1, 1 interactor |
STRINGi | 10090.ENSMUSP00000030775 |
Miscellaneous databases
RNActi | A2A8L1, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 499 – 556 | Chromo 1PROSITE-ProRule annotationAdd BLAST | 58 | |
Domaini | 594 – 655 | Chromo 2PROSITE-ProRule annotationAdd BLAST | 62 | |
Domaini | 714 – 898 | Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST | 185 | |
Domaini | 1030 – 1195 | Helicase C-terminalPROSITE-ProRule annotationAdd BLAST | 166 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 140 | DisorderedSequence analysisAdd BLAST | 140 | |
Regioni | 236 – 272 | DisorderedSequence analysisAdd BLAST | 37 | |
Regioni | 285 – 340 | DisorderedSequence analysisAdd BLAST | 56 | |
Regioni | 345 – 655 | Histone-bindingBy similarityAdd BLAST | 311 | |
Regioni | 551 – 573 | DisorderedSequence analysisAdd BLAST | 23 | |
Regioni | 1210 – 1254 | DisorderedSequence analysisAdd BLAST | 45 | |
Regioni | 1353 – 1413 | DisorderedSequence analysisAdd BLAST | 61 | |
Regioni | 1525 – 1566 | DisorderedSequence analysisAdd BLAST | 42 | |
Regioni | 1579 – 1696 | DisorderedSequence analysisAdd BLAST | 118 | |
Regioni | 1926 – 1946 | DisorderedSequence analysisAdd BLAST | 21 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 849 – 852 | DEAH box | 4 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 15 – 36 | Acidic residuesSequence analysisAdd BLAST | 22 | |
Compositional biasi | 99 – 114 | Basic residuesSequence analysisAdd BLAST | 16 | |
Compositional biasi | 115 – 132 | Basic and acidic residuesSequence analysisAdd BLAST | 18 | |
Compositional biasi | 244 – 259 | Basic and acidic residuesSequence analysisAdd BLAST | 16 | |
Compositional biasi | 1212 – 1232 | Polar residuesSequence analysisAdd BLAST | 21 | |
Compositional biasi | 1388 – 1410 | Basic and acidic residuesSequence analysisAdd BLAST | 23 | |
Compositional biasi | 1602 – 1696 | Basic and acidic residuesSequence analysisAdd BLAST | 95 |
Domaini
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 345 – 392 | PHD-type 1PROSITE-ProRule annotationAdd BLAST | 48 | |
Zinc fingeri | 418 – 465 | PHD-type 2PROSITE-ProRule annotationAdd BLAST | 48 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG0383, Eukaryota |
GeneTreei | ENSGT00940000159249 |
OrthoDBi | 709631at2759 |
PhylomeDBi | A2A8L1 |
Family and domain databases
Gene3Di | 3.30.40.10, 2 hits 3.40.50.10810, 1 hit 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR028727, CHD5 IPR012957, CHD_C2 IPR009462, CHD_II_SANT-like IPR012958, CHD_N IPR016197, Chromo-like_dom_sf IPR000953, Chromo/chromo_shadow_dom IPR023780, Chromo_domain IPR002464, DNA/RNA_helicase_DEAH_CS IPR009463, DUF1087 IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR038718, SNF2-like_sf IPR000330, SNF2_N IPR019786, Zinc_finger_PHD-type_CS IPR011011, Znf_FYVE_PHD IPR001965, Znf_PHD IPR019787, Znf_PHD-finger IPR013083, Znf_RING/FYVE/PHD |
PANTHERi | PTHR45623:SF6, PTHR45623:SF6, 1 hit |
Pfami | View protein in Pfam PF08074, CHDCT2, 1 hit PF08073, CHDNT, 1 hit PF00385, Chromo, 1 hit PF06461, DUF1086, 1 hit PF06465, DUF1087, 1 hit PF00271, Helicase_C, 1 hit PF00628, PHD, 2 hits PF00176, SNF2-rel_dom, 1 hit |
SMARTi | View protein in SMART SM00298, CHROMO, 2 hits SM00487, DEXDc, 1 hit SM01146, DUF1086, 1 hit SM01147, DUF1087, 1 hit SM00490, HELICc, 1 hit SM00249, PHD, 2 hits |
SUPFAMi | SSF52540, SSF52540, 2 hits SSF54160, SSF54160, 2 hits SSF57903, SSF57903, 1 hit |
PROSITEi | View protein in PROSITE PS50013, CHROMO_2, 2 hits PS00690, DEAH_ATP_HELICASE, 1 hit PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS01359, ZF_PHD_1, 2 hits PS50016, ZF_PHD_2, 2 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MRGPLGTEEE LPRLFAEEME NEEEMSEEED GGLEGFEDFF PAEPVSLPKK
60 70 80 90 100
KPKKLKESKS SKGKRKKKEG SNDEMSDNEE DLEEKSESEG SDYSPTKKKK
110 120 130 140 150
KKLKEKKEKK EKKEKRKKRG EDEDDNDDGG LKEPKSSGQL MAEWGLDDVD
160 170 180 190 200
YLFSEDDYHT LTNYKAFSQF LRPLIAKKNP KIPMSKMMTV LGAKWREFSA
210 220 230 240 250
NNPFKGSSAA AAAAAVAAAV ETVTIAPPLA ISPQQVPQTL PIRKAKTKEG
260 270 280 290 300
KGPGVRKKNK GAKDSKKKGR GKRVAGLKFR FGGISKRKKG SSSEEDERED
310 320 330 340 350
SDLDNASIHS SSVRSECSAA LGKKNKRRRK KKRIDDGDGY ETDHQDYCEV
360 370 380 390 400
CQQGGEIILC DTCPRAYHLV CLDPELEKAP EGKWSCPHCE KEGIQWEPKD
410 420 430 440 450
DDEEEEEGGC EEEEDDHMEF CRVCKDGGEL LCCDACPSSY HLHCLNPPLP
460 470 480 490 500
EIPNGEWLCP RCTCPPLKGK VQRILHWRWT EPPAPFVVGL PGPEVEPGMP
510 520 530 540 550
PPRPLEGIPE REFFVKWAGL SYWHCSWVKE LQLELYHTVM YRNYQRKNDM
560 570 580 590 600
DEPPPFDYGS GDEDGKSEKR KNKDPLYAKM EERFYRYGIK PEWMMVHRIL
610 620 630 640 650
NHSFDKKGDI HYLIKWKDLP YDQCTWEIDE IDIPYYDNLK QAYWGHRELM
660 670 680 690 700
LGEDARLPKR LVKKGKKLKD DKQEKPPDTP IVDPTVKFDK QPWYIDATGG
710 720 730 740 750
TLHPYQLEGL NWLRFSWAQG TDTILADEMG LGKTVQTIVF LYSLYKEGHS
760 770 780 790 800
KGPYLVSAPL STIINWEREF EMWAPDFYVV TYTGDKESRS VIRENEFSFE
810 820 830 840 850
DNAIRGGKKV FRMKKEVQIK FHVLLTSYEL ITIDQAILGS IEWACLVVDE
860 870 880 890 900
AHRLKNNQSK FFRVLNSYKI DYKLLLTGTP LQNNLEELFH LLNFLTPERF
910 920 930 940 950
NNLEGFLEEF ADISKEDQIK KLHDLLGPHM LRRLKADVFK NMPAKTELIV
960 970 980 990 1000
RVELSQMQKK YYKFILTRNF EALNSKGGGN QVSLLNIMMD LKKCCNHPYL
1010 1020 1030 1040 1050
FPVAAVEAPV LPNGSYDGSS LVKSSGKLML LQKMLKKLRD EGHRVLIFSQ
1060 1070 1080 1090 1100
MTKMLDLLED FLEYEGYKYE RIDGGITGGL RQEAIDRFNA PGAQQFCFLL
1110 1120 1130 1140 1150
STRAGGLGIN LATADTVIIY DSDWNPHNDI QAFSRAHRIG QNKKVMIYRF
1160 1170 1180 1190 1200
VTRASVEERI TQVAKRKMML THLVVRPGLG SKSGSMTKQE LDDILKFGTE
1210 1220 1230 1240 1250
ELFKDDVEGM MSQGQRPTTP IPDIQSTKGG SLTAGAKKKH GSTPPGDNKD
1260 1270 1280 1290 1300
VEDSSVIHYD DAAISKLLDR NQDATDDTEL QNMNEYLSSF KVAQYVVREE
1310 1320 1330 1340 1350
DGVEEVEREV IKQEENVDPD YWEKLLRHHY EQQQEDLARN LGKGKRIRKQ
1360 1370 1380 1390 1400
VNYNDASQED QEWQDELSDN QSEYSIGSED EDEDFEERPE GQSGRRQSRR
1410 1420 1430 1440 1450
QLKSDRDKPL PPLLARVGGN IEVLGFNARQ RKAFLNAIMR WGMPPQDAFN
1460 1470 1480 1490 1500
SHWLVRDLRG KSEKEFRAYV SLFMRHLCEP GADGAETFAD GVPREGLSRQ
1510 1520 1530 1540 1550
HVLTRIGVMS LVRKKVQEFE HVNGKYSTPD LVPEGAEGKK PGEVISSDPN
1560 1570 1580 1590 1600
TPVPASPAQL PPAPLGLTDK MEAQLGYTDE KESGMQKPKK SLEIQTLPTA
1610 1620 1630 1640 1650
LDRVEGEDKH QSSDSKDRAR EERTEEVEKA QGSPEQPLKE EVLPDKEPIP
1660 1670 1680 1690 1700
DKPELSLGHS GDFRPDDPKT EEKEPGETQQ NGDREEDEEG KKEDKNGKFK
1710 1720 1730 1740 1750
FMFNIADGGF TELHTLWQNE ERAAVSSGKI YEIWHRRHDY WLLAGIVTHG
1760 1770 1780 1790 1800
YARWQDIQND PRYMILNEPF KSEIHKGNYL EMKNKFLARR FKLLEQALVI
1810 1820 1830 1840 1850
EEQLRRAAYL NMTQDPNHPA MALNARLAEV ECLAESHQHL SKESLAGNKP
1860 1870 1880 1890 1900
ANAVLHKVLN QLEELLSDMK ADVTRLPSML SRIPPVAARL QMSERSILSR
1910 1920 1930 1940
LTNRAGDPTI QQTSSRRRDF PLFQRSFPAE PSHLPNPRGR EKLQPF
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9PYL1 | E9PYL1_MOUSE | DNA helicase | Chd5 | 1,952 | Annotation score: | ||
E9PYU4 | E9PYU4_MOUSE | DNA helicase | Chd5 | 1,915 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL611985 Genomic DNA No translation available. AK129145 mRNA Translation: BAC97955.1 |
CCDSi | CCDS89866.1 |
RefSeqi | XP_006538995.1, XM_006538932.2 |
Genome annotation databases
Ensembli | ENSMUST00000005175; ENSMUSP00000005175; ENSMUSG00000005045 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL611985 Genomic DNA No translation available. AK129145 mRNA Translation: BAC97955.1 |
CCDSi | CCDS89866.1 |
RefSeqi | XP_006538995.1, XM_006538932.2 |
3D structure databases
SMRi | A2A8L1 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 234678, 11 interactors |
IntActi | A2A8L1, 1 interactor |
STRINGi | 10090.ENSMUSP00000030775 |
PTM databases
iPTMneti | A2A8L1 |
PhosphoSitePlusi | A2A8L1 |
Proteomic databases
jPOSTi | A2A8L1 |
MaxQBi | A2A8L1 |
PaxDbi | A2A8L1 |
PeptideAtlasi | A2A8L1 |
PRIDEi | A2A8L1 |
ProteomicsDBi | 281603 |
Protocols and materials databases
Antibodypediai | 27125, 139 antibodies from 29 providers |
Genome annotation databases
Ensembli | ENSMUST00000005175; ENSMUSP00000005175; ENSMUSG00000005045 |
Organism-specific databases
MGIi | MGI:3036258, Chd5 |
VEuPathDBi | HostDB:ENSMUSG00000005045 |
Rougei | Search... |
Phylogenomic databases
eggNOGi | KOG0383, Eukaryota |
GeneTreei | ENSGT00940000159249 |
OrthoDBi | 709631at2759 |
PhylomeDBi | A2A8L1 |
Miscellaneous databases
BioGRID-ORCSi | 269610, 5 hits in 65 CRISPR screens |
PROi | PR:A2A8L1 |
RNActi | A2A8L1, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000005045, Expressed in primary visual cortex and 163 other tissues |
ExpressionAtlasi | A2A8L1, baseline and differential |
Genevisiblei | A2A8L1, MM |
Family and domain databases
Gene3Di | 3.30.40.10, 2 hits 3.40.50.10810, 1 hit 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR028727, CHD5 IPR012957, CHD_C2 IPR009462, CHD_II_SANT-like IPR012958, CHD_N IPR016197, Chromo-like_dom_sf IPR000953, Chromo/chromo_shadow_dom IPR023780, Chromo_domain IPR002464, DNA/RNA_helicase_DEAH_CS IPR009463, DUF1087 IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR038718, SNF2-like_sf IPR000330, SNF2_N IPR019786, Zinc_finger_PHD-type_CS IPR011011, Znf_FYVE_PHD IPR001965, Znf_PHD IPR019787, Znf_PHD-finger IPR013083, Znf_RING/FYVE/PHD |
PANTHERi | PTHR45623:SF6, PTHR45623:SF6, 1 hit |
Pfami | View protein in Pfam PF08074, CHDCT2, 1 hit PF08073, CHDNT, 1 hit PF00385, Chromo, 1 hit PF06461, DUF1086, 1 hit PF06465, DUF1087, 1 hit PF00271, Helicase_C, 1 hit PF00628, PHD, 2 hits PF00176, SNF2-rel_dom, 1 hit |
SMARTi | View protein in SMART SM00298, CHROMO, 2 hits SM00487, DEXDc, 1 hit SM01146, DUF1086, 1 hit SM01147, DUF1087, 1 hit SM00490, HELICc, 1 hit SM00249, PHD, 2 hits |
SUPFAMi | SSF52540, SSF52540, 2 hits SSF54160, SSF54160, 2 hits SSF57903, SSF57903, 1 hit |
PROSITEi | View protein in PROSITE PS50013, CHROMO_2, 2 hits PS00690, DEAH_ATP_HELICASE, 1 hit PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS01359, ZF_PHD_1, 2 hits PS50016, ZF_PHD_2, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | CHD5_MOUSE | |
Accessioni | A2A8L1Primary (citable) accession number: A2A8L1 Secondary accession number(s): Q6ZQB2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 11, 2014 |
Last sequence update: | February 20, 2007 | |
Last modified: | February 23, 2022 | |
This is version 123 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families