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Protein

E3 ubiquitin-protein ligase RNF19B

Gene

Rnf19b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates, such as UCKL1. Involved in the cytolytic activity of natural killer cells and cytotoxic T-cells. Protects against staurosporin-induced cell death (By similarity).By similarity

Catalytic activityi

[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine.By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi116Zinc 1PROSITE-ProRule annotation1
Metal bindingi119Zinc 1PROSITE-ProRule annotation1
Metal bindingi139Zinc 1PROSITE-ProRule annotation1
Metal bindingi142Zinc 1PROSITE-ProRule annotation1
Metal bindingi203Zinc 2PROSITE-ProRule annotation1
Metal bindingi208Zinc 2PROSITE-ProRule annotation1
Metal bindingi225Zinc 2PROSITE-ProRule annotation1
Metal bindingi230Zinc 2PROSITE-ProRule annotation1
Metal bindingi235Zinc 3PROSITE-ProRule annotation1
Metal bindingi238Zinc 3PROSITE-ProRule annotation1
Metal bindingi243Zinc 3; via tele nitrogenPROSITE-ProRule annotation1
Metal bindingi248Zinc 3PROSITE-ProRule annotation1
Metal bindingi284Zinc 4PROSITE-ProRule annotation1
Metal bindingi287Zinc 4PROSITE-ProRule annotation1
Active sitei299PROSITE-ProRule annotation1
Metal bindingi304Zinc 4PROSITE-ProRule annotation1
Metal bindingi307Zinc 4PROSITE-ProRule annotation1
Metal bindingi312Zinc 5PROSITE-ProRule annotation1
Metal bindingi315Zinc 5PROSITE-ProRule annotation1
Metal bindingi323Zinc 5; via tele nitrogenPROSITE-ProRule annotation1
Metal bindingi330Zinc 5PROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri116 – 165RING-type 1PROSITE-ProRule annotationAdd BLAST50
Zinc fingeri183 – 248IBR-typePROSITE-ProRule annotationAdd BLAST66
Zinc fingeri284 – 315RING-type 2; atypicalPROSITE-ProRule annotationAdd BLAST32

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processAdaptive immunity, Immunity, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase RNF19B (EC:2.3.2.31By similarity)
Alternative name(s):
IBR domain-containing protein 3
Natural killer lytic-associated molecule
RING finger protein 19B
Gene namesi
Name:Rnf19b
Synonyms:Ibrdc3, Nklam
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1922484 Rnf19b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei351 – 371HelicalSequence analysisAdd BLAST21
Transmembranei412 – 432HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003071881 – 732E3 ubiquitin-protein ligase RNF19BAdd BLAST732

Proteomic databases

PaxDbiA2A7Q9
PRIDEiA2A7Q9

PTM databases

iPTMnetiA2A7Q9
PhosphoSitePlusiA2A7Q9

Expressioni

Tissue specificityi

Expressed specifically in natural killer cells, activated macrophages and cytotoxic T-cells (PubMed:10912506). Present in macrophages (at protein level) (PubMed:10912506). Ubiquitously expressed with high expression in testis (PubMed:27485036).2 Publications

Inductioni

By IFNG and LPS in macrophages. By IL2 in natural killer cells and cytotoxic T-cells.1 Publication

Gene expression databases

BgeeiENSMUSG00000028793 Expressed in 269 organ(s), highest expression level in testis
CleanExiMM_RNF19B
ExpressionAtlasiA2A7Q9 baseline and differential
GenevisibleiA2A7Q9 MM

Interactioni

Subunit structurei

Interacts with UBE2L3, UBE2L6 and UCKL1.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi217322, 2 interactors
STRINGi10090.ENSMUSP00000131373

Structurei

3D structure databases

ProteinModelPortaliA2A7Q9
SMRiA2A7Q9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 315Required for ubiquitin ligase activity and for protection against staurosporin-induced cell deathBy similarityAdd BLAST315
Regioni112 – 334TRIAD supradomainPROSITE-ProRule annotationAdd BLAST223

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi29 – 32Poly-Arg4
Compositional biasi52 – 87Pro-richAdd BLAST36

Domaini

The first IBR-type zinc finger is the most crucial for interaction with UBE2L3, UBE2L6 and UCKL1.By similarity
Members of the RBR family are atypical E3 ligases. They interact with the E2 conjugating enzyme UBE2L3 and function like HECT-type E3 enzymes: they bind E2s via the first RING domain, but require an obligate trans-thiolation step during the ubiquitin transfer, requiring a conserved cysteine residue in the second RING domain.By similarity

Sequence similaritiesi

Belongs to the RBR family. RNF19 subfamily.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri116 – 165RING-type 1PROSITE-ProRule annotationAdd BLAST50
Zinc fingeri183 – 248IBR-typePROSITE-ProRule annotationAdd BLAST66
Zinc fingeri284 – 315RING-type 2; atypicalPROSITE-ProRule annotationAdd BLAST32

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

eggNOGiKOG1815 Eukaryota
ENOG410XP9Y LUCA
GeneTreeiENSGT00840000129738
HOGENOMiHOG000059258
HOVERGENiHBG052073
InParanoidiA2A7Q9
OMAiPLCLVRQ
OrthoDBiEOG091G0503
PhylomeDBiA2A7Q9
TreeFamiTF324777

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR031127 E3_UB_ligase_RBR
IPR002867 IBR_dom
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
PANTHERiPTHR11685 PTHR11685, 1 hit
PfamiView protein in Pfam
PF01485 IBR, 2 hits
SMARTiView protein in SMART
SM00647 IBR, 2 hits
SM00184 RING, 1 hit
PROSITEiView protein in PROSITE
PS51873 TRIAD, 1 hit
PS50089 ZF_RING_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All

A2A7Q9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGSEKDSESP RSTSLHAAAP DPKCRSGGRR RRLTFHSVFS ASARGRRART
60 70 80 90 100
KPQAEPPPPA APPPPPPPAP APVEAQAPPV EALPSEPAAE AEAEAVAAGP
110 120 130 140 150
EEDEAAEGGG AEEVECPLCL VRLPPERAPR LLSCPHRSCR DCLRHYLRLE
160 170 180 190 200
ISESRVPISC PECSERLNPH DIRLLLADPP LMHKYEEFML RRYLASDPDC
210 220 230 240 250
RWCPAPDCGY AVIAYGCASC PKLTCEREGC QTEFCYHCKQ IWHPNQTCDM
260 270 280 290 300
ARQQRAQTLR VRTKHTSGLS YGQESGPADD IKPCPRCSAY IIKMNDGSCN
310 320 330 340 350
HMTCAVCGCE FCWLCMKEIS DLHYLSPSGC TFWGKKPWSR KKKILWQLGT
360 370 380 390 400
LIGAPVGISL IAGIAIPAMV IGIPVYVGRK IHSRYEGRKT SKHKRNLAIT
410 420 430 440 450
GGVTLSVIAS PVIAAVSVGI GVPIMLAYVY GVVPISLCRG GGCGVSTANG
460 470 480 490 500
KGVKIEFDED DGPITVADAW RALKNPSIGE SSIEGLTSVL STSGSPTDGL
510 520 530 540 550
SVMQGPYSET ASFAALSGGT LSGGILSSGK GKYSRLEVQA DVQKEIFPKD
560 570 580 590 600
TASLGAISDS ASTRAMAGSI ISSYNPQDRE CNNMEIQVDI EAKPSHYQLV
610 620 630 640 650
SGSSTEDSLH VHAQVAEKEE EGNGAGGGSG GSEDDPPYKH QSCEQKDCLA
660 670 680 690 700
SKAWDISLAQ PESIRSDLES SDTQSDDVPD ITSDECGSPR SHAAACPSTP
710 720 730
QVHGAPSPSA HKNLAAPAEG QTVLKSEEYE VE
Length:732
Mass (Da):78,127
Last modified:October 23, 2007 - v2
Checksum:i81AAF64F9AE566F7
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9Q2L7E9Q2L7_MOUSE
RBR-type E3 ubiquitin transferase
Rnf19b
731Annotation score:
B2KG08B2KG08_MOUSE
RBR-type E3 ubiquitin transferase
Rnf19b
551Annotation score:
A2A7Q8A2A7Q8_MOUSE
RBR-type E3 ubiquitin transferase
Rnf19b
352Annotation score:
F6TQN1F6TQN1_MOUSE
E3 ubiquitin-protein ligase RNF19B
Rnf19b
247Annotation score:

Sequence cautioni

The sequence AAH52529 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAM19707 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti278Missing (PubMed:10912506).Curated1
Sequence conflicti278Missing in AAI17808 (PubMed:15489334).Curated1
Sequence conflicti721Q → P in AAH52529 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL606977 Genomic DNA Translation: CAM19707.1 Different initiation.
BC052529 mRNA Translation: AAH52529.1 Different initiation.
BC117807 mRNA Translation: AAI17808.1
RefSeqiXP_006503521.1, XM_006503458.3
UniGeneiMm.259672
Mm.486170

Genome annotation databases

EnsembliENSMUST00000030584; ENSMUSP00000030584; ENSMUSG00000028793
GeneIDi75234
UCSCiuc008uvx.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL606977 Genomic DNA Translation: CAM19707.1 Different initiation.
BC052529 mRNA Translation: AAH52529.1 Different initiation.
BC117807 mRNA Translation: AAI17808.1
RefSeqiXP_006503521.1, XM_006503458.3
UniGeneiMm.259672
Mm.486170

3D structure databases

ProteinModelPortaliA2A7Q9
SMRiA2A7Q9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi217322, 2 interactors
STRINGi10090.ENSMUSP00000131373

PTM databases

iPTMnetiA2A7Q9
PhosphoSitePlusiA2A7Q9

Proteomic databases

PaxDbiA2A7Q9
PRIDEiA2A7Q9

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030584; ENSMUSP00000030584; ENSMUSG00000028793
GeneIDi75234
UCSCiuc008uvx.2 mouse

Organism-specific databases

CTDi127544
MGIiMGI:1922484 Rnf19b

Phylogenomic databases

eggNOGiKOG1815 Eukaryota
ENOG410XP9Y LUCA
GeneTreeiENSGT00840000129738
HOGENOMiHOG000059258
HOVERGENiHBG052073
InParanoidiA2A7Q9
OMAiPLCLVRQ
OrthoDBiEOG091G0503
PhylomeDBiA2A7Q9
TreeFamiTF324777

Enzyme and pathway databases

UniPathwayi
UPA00143

ReactomeiR-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation

Miscellaneous databases

ChiTaRSiRnf19b mouse
PROiPR:A2A7Q9
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028793 Expressed in 269 organ(s), highest expression level in testis
CleanExiMM_RNF19B
ExpressionAtlasiA2A7Q9 baseline and differential
GenevisibleiA2A7Q9 MM

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR031127 E3_UB_ligase_RBR
IPR002867 IBR_dom
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
PANTHERiPTHR11685 PTHR11685, 1 hit
PfamiView protein in Pfam
PF01485 IBR, 2 hits
SMARTiView protein in SMART
SM00647 IBR, 2 hits
SM00184 RING, 1 hit
PROSITEiView protein in PROSITE
PS51873 TRIAD, 1 hit
PS50089 ZF_RING_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRN19B_MOUSE
AccessioniPrimary (citable) accession number: A2A7Q9
Secondary accession number(s): A0AUN9, Q7TT05
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: October 23, 2007
Last modified: November 7, 2018
This is version 92 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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