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Protein

Cell division cycle protein 27 homolog

Gene

Cdc27

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-MMU-141430 Inactivation of APC/C via direct inhibition of the APC/C complex
R-MMU-174048 APC/C:Cdc20 mediated degradation of Cyclin B
R-MMU-174084 Autodegradation of Cdh1 by Cdh1:APC/C
R-MMU-174154 APC/C:Cdc20 mediated degradation of Securin
R-MMU-174178 APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1
R-MMU-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-MMU-176407 Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase
R-MMU-176408 Regulation of APC/C activators between G1/S and early anaphase
R-MMU-176409 APC/C:Cdc20 mediated degradation of mitotic proteins
R-MMU-176412 Phosphorylation of the APC/C
R-MMU-179409 APC-Cdc20 mediated degradation of Nek2A
R-MMU-2467813 Separation of Sister Chromatids
R-MMU-2559582 Senescence-Associated Secretory Phenotype (SASP)
R-MMU-69017 CDK-mediated phosphorylation and removal of Cdc6
R-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Cell division cycle protein 27 homolog
Gene namesi
Name:Cdc27
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:102685 Cdc27

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003904731 – 825Cell division cycle protein 27 homologAdd BLAST825

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei205PhosphothreonineBy similarity1
Modified residuei209PhosphothreonineBy similarity1
Modified residuei291PhosphoserineBy similarity1
Modified residuei313PhosphothreonineBy similarity1
Modified residuei339PhosphoserineBy similarity1
Modified residuei367PhosphothreonineBy similarity1
Modified residuei380PhosphoserineBy similarity1
Modified residuei387PhosphoserineBy similarity1
Modified residuei427PhosphoserineBy similarity1
Modified residuei431PhosphothreonineBy similarity1
Modified residuei436PhosphoserineBy similarity1
Modified residuei439PhosphoserineBy similarity1
Modified residuei447PhosphothreonineBy similarity1
Modified residuei822PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated. Phosphorylation on Ser-427 and Thr-447 occurs specifically during mitosis (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiA2A6Q5
MaxQBiA2A6Q5
PaxDbiA2A6Q5
PeptideAtlasiA2A6Q5
PRIDEiA2A6Q5

PTM databases

iPTMnetiA2A6Q5
PhosphoSitePlusiA2A6Q5

Expressioni

Gene expression databases

BgeeiENSMUSG00000020687 Expressed in 248 organ(s), highest expression level in cumulus cell
ExpressionAtlasiA2A6Q5 baseline and differential
GenevisibleiA2A6Q5 MM

Interactioni

Subunit structurei

Homodimer. The mammalian APC/C is composed at least of 14 distinct subunits ANAPC1, ANAPC2, CDC27/APC3, ANAPC4, ANAPC5, CDC16/APC6, ANAPC7, CDC23/APC8, ANAPC10, ANAPC11, CDC26/APC12, ANAPC13, ANAPC15 and ANAPC16 that assemble into a complex of at least 19 chains with a combined molecular mass of around 1.2 MDa; APC/C interacts with FZR1 and FBXO5 (By similarity). Interacts with RB. Interacts with FAM168B/MANI (PubMed:20716133). Interacts with MCPH1 (By similarity).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi229869, 7 interactors
DIPiDIP-56609N
IntActiA2A6Q5, 4 interactors
STRINGi10090.ENSMUSP00000091452

Structurei

3D structure databases

ProteinModelPortaliA2A6Q5
SMRiA2A6Q5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati6 – 35TPR 1Add BLAST30
Repeati38 – 65TPR 2Add BLAST28
Repeati67 – 99TPR 3Add BLAST33
Repeati115 – 145TPR 4Add BLAST31
Repeati465 – 495TPR 5Add BLAST31
Repeati499 – 528TPR 6Add BLAST30
Repeati533 – 563TPR 7Add BLAST31
Repeati567 – 598TPR 8Add BLAST32
Repeati601 – 631TPR 9Add BLAST31
Repeati635 – 667TPR 10Add BLAST33
Repeati670 – 702TPR 11Add BLAST33
Repeati704 – 734TPR 12Add BLAST31
Repeati737 – 768TPR 13Add BLAST32

Sequence similaritiesi

Belongs to the APC3/CDC27 family.Curated

Keywords - Domaini

Repeat, TPR repeat

Phylogenomic databases

eggNOGiKOG1126 Eukaryota
COG0457 LUCA
GeneTreeiENSGT00550000074887
HOGENOMiHOG000231056
HOVERGENiHBG050859
InParanoidiA2A6Q5
KOiK03350
OMAiMPLTSPC
OrthoDBiEOG091G03JF
PhylomeDBiA2A6Q5
TreeFamiTF101058

Family and domain databases

Gene3Di1.25.40.10, 4 hits
InterProiView protein in InterPro
IPR013026 TPR-contain_dom
IPR011990 TPR-like_helical_dom_sf
IPR001440 TPR_1
IPR019734 TPR_repeat
PfamiView protein in Pfam
PF00515 TPR_1, 2 hits
PF13181 TPR_8, 2 hits
SMARTiView protein in SMART
SM00028 TPR, 8 hits
SUPFAMiSSF48452 SSF48452, 2 hits
PROSITEiView protein in PROSITE
PS50005 TPR, 8 hits
PS50293 TPR_REGION, 2 hits

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: A2A6Q5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MTVLQEPVQA AIWQALNHYA YRDAVFLAER LYAEVHSEEA LFLLATCYYR
60 70 80 90 100
SGKAYKAYRL LKGHSCTTPQ CKYLLAKCCV DLSKLAEGEQ ILSGGVFNKQ
110 120 130 140 150
KSHDDLVTEF GDSACFTLSL LGHVYCKTDR LAKGSECYQK SLSLNPFLWS
160 170 180 190 200
PFESLCEIGE KPDPDQTFKL TSLQNFSSCL PNTCTTLVSN HSLSHRQPET
210 220 230 240 250
VLTETPQDTI ELNRLNLESS NSKYSLNTDS SVSYIDSTVI SPDNVPLGPG
260 270 280 290 300
TAILSKQVQN KPKTGRSLLG GPTALSPLTP SFGILPLETP SPGDGSYLQN
310 320 330 340 350
YTNTPSVIDV APTGAPTKKS VARMGQTGTK SVFSQSGNSR EVTPVLVAQT
360 370 380 390 400
QSSGPQTSTT PQVLSPTITS PPNALPRRSS RLFTSDSSTT KENSKKLKMK
410 420 430 440 450
FPPKIPNRKT KSKTNKGGLT QPSINDSLEI TKLDSSIISE GKITTVTPQI
460 470 480 490 500
QAFNLQKAAA EGLMSLLREM GKGYLALCSY NCKEAINILS HLPSHHYSTG
510 520 530 540 550
WVLCQIGRAY FELSEYMQAE RIFSEVRRIE SFRVEGMEIY STTLWHLQKD
560 570 580 590 600
VALSVLSKDL TDMDKNSPEA WCAAGNCFSL QREHDIAIKF FQRAIQVDPN
610 620 630 640 650
YAYAYTLLGH EFVLTEELDK ALACFRNAIR VNPRHYNAWY GLGMIYYKQE
660 670 680 690 700
KFSLAEMHFQ KALDINPQSS VLLCHIGVVQ HALKKSEKAL DTLNKAIVID
710 720 730 740 750
PKNPLCKFHR ASVLFANEKY KSALQELEEL KQIVPKESLV YFLIGKVYKK
760 770 780 790 800
LGQTHLALMN FSWAMDLDPK GANNQIKEAI DKRYLPDDEE PITQEEQIMG
810 820
TDESQESSMT DADDTQLHAA ESDEF
Length:825
Mass (Da):91,842
Last modified:February 20, 2007 - v1
Checksum:i554D8B1136826DA3
GO
Isoform 2 (identifier: A2A6Q5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     319-319: K → KTFCVLQ
     461-461: Missing.

Note: No experimental confirmation available.
Show »
Length:830
Mass (Da):92,405
Checksum:i28AD6139A08823F5
GO
Isoform 3 (identifier: A2A6Q5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     319-319: K → KTFCVLQ

Note: No experimental confirmation available.
Show »
Length:831
Mass (Da):92,534
Checksum:iFCFAEA4338F99917
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q8R568Q8R568_MOUSE
Cell division cycle 27 homolog (S. ...
Cdc27
399Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_038534319K → KTFCVLQ in isoform 2 and isoform 3. 1 Publication1
Alternative sequenceiVSP_038535461Missing in isoform 2. Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL603709 Genomic DNA Translation: CAM22621.1
AL603709 Genomic DNA Translation: CAM22622.1
CH466558 Genomic DNA Translation: EDL34221.1
BC157955 mRNA Translation: AAI57956.1
BC172100 mRNA Translation: AAI72100.1
CCDSiCCDS36354.1 [A2A6Q5-1]
RefSeqiNP_001272917.1, NM_001285988.1 [A2A6Q5-2]
NP_663411.2, NM_145436.2 [A2A6Q5-1]
XP_006533113.1, XM_006533050.2 [A2A6Q5-3]
UniGeneiMm.89845

Genome annotation databases

EnsembliENSMUST00000093923; ENSMUSP00000091452; ENSMUSG00000020687 [A2A6Q5-1]
ENSMUST00000106962; ENSMUSP00000102575; ENSMUSG00000020687 [A2A6Q5-3]
GeneIDi217232
KEGGimmu:217232
UCSCiuc007lwq.2 mouse [A2A6Q5-1]
uc007lwr.2 mouse [A2A6Q5-2]
uc011ygc.2 mouse [A2A6Q5-3]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL603709 Genomic DNA Translation: CAM22621.1
AL603709 Genomic DNA Translation: CAM22622.1
CH466558 Genomic DNA Translation: EDL34221.1
BC157955 mRNA Translation: AAI57956.1
BC172100 mRNA Translation: AAI72100.1
CCDSiCCDS36354.1 [A2A6Q5-1]
RefSeqiNP_001272917.1, NM_001285988.1 [A2A6Q5-2]
NP_663411.2, NM_145436.2 [A2A6Q5-1]
XP_006533113.1, XM_006533050.2 [A2A6Q5-3]
UniGeneiMm.89845

3D structure databases

ProteinModelPortaliA2A6Q5
SMRiA2A6Q5
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi229869, 7 interactors
DIPiDIP-56609N
IntActiA2A6Q5, 4 interactors
STRINGi10090.ENSMUSP00000091452

PTM databases

iPTMnetiA2A6Q5
PhosphoSitePlusiA2A6Q5

Proteomic databases

EPDiA2A6Q5
MaxQBiA2A6Q5
PaxDbiA2A6Q5
PeptideAtlasiA2A6Q5
PRIDEiA2A6Q5

Protocols and materials databases

DNASUi217232
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000093923; ENSMUSP00000091452; ENSMUSG00000020687 [A2A6Q5-1]
ENSMUST00000106962; ENSMUSP00000102575; ENSMUSG00000020687 [A2A6Q5-3]
GeneIDi217232
KEGGimmu:217232
UCSCiuc007lwq.2 mouse [A2A6Q5-1]
uc007lwr.2 mouse [A2A6Q5-2]
uc011ygc.2 mouse [A2A6Q5-3]

Organism-specific databases

CTDi996
MGIiMGI:102685 Cdc27

Phylogenomic databases

eggNOGiKOG1126 Eukaryota
COG0457 LUCA
GeneTreeiENSGT00550000074887
HOGENOMiHOG000231056
HOVERGENiHBG050859
InParanoidiA2A6Q5
KOiK03350
OMAiMPLTSPC
OrthoDBiEOG091G03JF
PhylomeDBiA2A6Q5
TreeFamiTF101058

Enzyme and pathway databases

UniPathwayi
UPA00143

ReactomeiR-MMU-141430 Inactivation of APC/C via direct inhibition of the APC/C complex
R-MMU-174048 APC/C:Cdc20 mediated degradation of Cyclin B
R-MMU-174084 Autodegradation of Cdh1 by Cdh1:APC/C
R-MMU-174154 APC/C:Cdc20 mediated degradation of Securin
R-MMU-174178 APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1
R-MMU-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-MMU-176407 Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase
R-MMU-176408 Regulation of APC/C activators between G1/S and early anaphase
R-MMU-176409 APC/C:Cdc20 mediated degradation of mitotic proteins
R-MMU-176412 Phosphorylation of the APC/C
R-MMU-179409 APC-Cdc20 mediated degradation of Nek2A
R-MMU-2467813 Separation of Sister Chromatids
R-MMU-2559582 Senescence-Associated Secretory Phenotype (SASP)
R-MMU-69017 CDK-mediated phosphorylation and removal of Cdc6
R-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation

Miscellaneous databases

PROiPR:A2A6Q5
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020687 Expressed in 248 organ(s), highest expression level in cumulus cell
ExpressionAtlasiA2A6Q5 baseline and differential
GenevisibleiA2A6Q5 MM

Family and domain databases

Gene3Di1.25.40.10, 4 hits
InterProiView protein in InterPro
IPR013026 TPR-contain_dom
IPR011990 TPR-like_helical_dom_sf
IPR001440 TPR_1
IPR019734 TPR_repeat
PfamiView protein in Pfam
PF00515 TPR_1, 2 hits
PF13181 TPR_8, 2 hits
SMARTiView protein in SMART
SM00028 TPR, 8 hits
SUPFAMiSSF48452 SSF48452, 2 hits
PROSITEiView protein in PROSITE
PS50005 TPR, 8 hits
PS50293 TPR_REGION, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiCDC27_MOUSE
AccessioniPrimary (citable) accession number: A2A6Q5
Secondary accession number(s): A2A6Q6, B2RXS0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: February 20, 2007
Last modified: November 7, 2018
This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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