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Protein

Methylosome subunit pICln

Gene

icln

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Chaperone that regulates the assembly of spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus (By similarity).By similarity1 Publication

GO - Biological processi

Keywordsi

Molecular functionChaperone

Names & Taxonomyi

Protein namesi
Recommended name:
Methylosome subunit pICln
Gene namesi
Name:icln
ORF Names:CG4924
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0029079 icln

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004254121 – 215Methylosome subunit pIClnAdd BLAST215

Proteomic databases

PaxDbiA1ZAW5
PRIDEiA1ZAW5

Expressioni

Gene expression databases

BgeeiFBgn0029079
ExpressionAtlasiA1ZAW5 baseline and differential
GenevisibleiA1ZAW5 DM

Interactioni

Subunit structurei

Component of the methylosome, a 20S complex containing at least CLNS1A/pICln, PRMT5/SKB1 and WDR77/MEP50; may mediate SNRPD1 and SNRPD3 methylation. Forms a 6S pICln-Sm complex composed of CLNS1A/pICln, SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG; ring-like structure where CLNS1A/pICln mimics additional Sm proteins and which is unable to assemble into the core snRNP.1 Publication

Protein-protein interaction databases

BioGridi62685, 14 interactors
IntActiA1ZAW5, 1 interactor
STRINGi7227.FBpp0086010

Structurei

Secondary structure

1215
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 5Combined sources4
Beta strandi14 – 27Combined sources14
Beta strandi29 – 50Combined sources22
Beta strandi52 – 56Combined sources5
Helixi57 – 59Combined sources3
Beta strandi60 – 79Combined sources20
Turni84 – 86Combined sources3
Beta strandi133 – 141Combined sources9
Helixi145 – 159Combined sources15

3D structure databases

ProteinModelPortaliA1ZAW5
SMRiA1ZAW5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3238 Eukaryota
ENOG4111R94 LUCA
InParanoidiA1ZAW5
KOiK05019
OMAiCIYFMLD
OrthoDBiEOG091G0NJR
PhylomeDBiA1ZAW5

Family and domain databases

InterProiView protein in InterPro
IPR003521 ICln
PRINTSiPR01348 ICLNCHANNEL

Sequencei

Sequence statusi: Complete.

A1ZAW5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLIMRVSPP EHGLLYTANN IKLKLGDKVV GEGTVYIAQN TLSWQPTELA
60 70 80 90 100
EGISIEWKQV SLHGISSNPR KCIYFMLDHK VEWNGVYGDP PQQAVNGRNG
110 120 130 140 150
GGSEAEVDEG NGSDEHDEDD NFEDAVDEQF GEVTECWLMP EDIHTVDTMY
160 170 180 190 200
SAMTTCQALH PDSANSDSED SDPMQDAGGL EDEAMEEDDA LTLGRNGVQN
210
LSLDDDEERF EDADE
Length:215
Mass (Da):23,743
Last modified:February 6, 2007 - v1
Checksum:iAD92706F7891D800
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti6R → H in AAF21126 (Ref. 1) Curated1
Sequence conflicti6R → H in AAL89952 (PubMed:12537569).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF216522 mRNA Translation: AAF21126.1
AE013599 Genomic DNA Translation: AAF57826.1
AY084214 mRNA Translation: AAL89952.1
RefSeqiNP_611237.2, NM_137393.4
UniGeneiDm.6477

Genome annotation databases

EnsemblMetazoaiFBtr0086832; FBpp0086010; FBgn0029079
GeneIDi36997
KEGGidme:Dmel_CG4924
UCSCiCG4924-RA d. melanogaster

Similar proteinsi

Entry informationi

Entry nameiICLN_DROME
AccessioniPrimary (citable) accession number: A1ZAW5
Secondary accession number(s): Q9U3W1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 19, 2014
Last sequence update: February 6, 2007
Last modified: May 23, 2018
This is version 86 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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