UniProtKB - A1Z9X0 (APKC_DROME)
Atypical protein kinase C
aPKC
Functioni
Serine/threonine protein kinase which is required for apico-basal cell polarity in the germ line as well as in epithelial and neural precursor cells, for epithelial planar cell polarity and for cell proliferation. During oocyte development, required for the posterior translocation of oocyte specification factors and for the posterior establishment of the microtubule organizing center within the presumptive oocyte. Phosphorylates l2gl which restricts l2gl activity to the oocyte posterior and regulates posterior enrichment of par-1, leading to establishment of correct oocyte polarity. Essential for apical localization of l2gl and par-6 in neuroblasts and for exclusion of mira from the apical cortex. Phosphorylates baz which is required for targeting of baz to the postsynaptic region where it is involved in actin organization, and for apical exclusion of baz which is necessary for establishment of the apical/lateral border in epithelial cells. Phosphorylates yrt which prevents its premature apical localization and is necessary for correct epithelial cell polarization. Required for the establishment of mitotic spindle orientation during symmetric division of epithelial cells and for apical exclusion of raps/Pins. Involved in symmetric adherens junction positioning during embryogenesis. Required for polarization of the spermatid cyst which is necessary for sperm differentiation. Required for stimulation of the Toll signaling pathway which activates Dif and dl and plays a role in innate immunity. Plays a role in memory enhancement.
15 PublicationsCatalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 293 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 388 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 145 – 195 | Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST | 51 | |
Nucleotide bindingi | 270 – 278 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- calcium-dependent protein kinase C activity Source: UniProtKB-EC
- metal ion binding Source: UniProtKB-KW
- myosin binding Source: FlyBase
- protein kinase C activity Source: UniProtKB
- protein serine/threonine/tyrosine kinase activity Source: RHEA
- protein serine/threonine kinase activity Source: FlyBase
- protein serine kinase activity Source: RHEA
GO - Biological processi
- adherens junction organization Source: UniProtKB
- apical protein localization Source: FlyBase
- asymmetric neuroblast division Source: FlyBase
- asymmetric protein localization involved in cell fate determination Source: FlyBase
- branching involved in open tracheal system development Source: FlyBase
- cell-cell junction assembly Source: FlyBase
- compound eye retinal cell programmed cell death Source: FlyBase
- epithelial cell morphogenesis Source: FlyBase
- establishment of epithelial cell apical/basal polarity Source: FlyBase
- establishment of epithelial cell planar polarity Source: FlyBase
- establishment of mitotic spindle orientation Source: FlyBase
- establishment or maintenance of cell polarity Source: FlyBase
- establishment or maintenance of epithelial cell apical/basal polarity Source: FlyBase
- establishment or maintenance of neuroblast polarity Source: FlyBase
- establishment or maintenance of polarity of embryonic epithelium Source: FlyBase
- establishment or maintenance of polarity of follicular epithelium Source: FlyBase
- exocyst localization Source: FlyBase
- germarium-derived oocyte fate determination Source: FlyBase
- intracellular signal transduction Source: GO_Central
- maintenance of cell polarity Source: FlyBase
- melanotic encapsulation of foreign target Source: FlyBase
- memory Source: FlyBase
- morphogenesis of a polarized epithelium Source: FlyBase
- negative regulation of hippo signaling Source: FlyBase
- nuclear envelope budding Source: FlyBase
- oocyte anterior/posterior axis specification Source: FlyBase
- peptidyl-serine phosphorylation Source: GO_Central
- positive regulation of filopodium assembly Source: FlyBase
- positive regulation of lamellipodium assembly Source: FlyBase
- positive regulation of neuroblast proliferation Source: FlyBase
- positive regulation of smoothened signaling pathway Source: FlyBase
- protein localization to plasma membrane Source: FlyBase
- protein phosphorylation Source: FlyBase
- regulation of intracellular mRNA localization Source: FlyBase
- regulation of polarized epithelial cell differentiation Source: FlyBase
- sensory organ development Source: FlyBase
- spermatogenesis Source: FlyBase
- synapse assembly Source: FlyBase
- terminal branching, open tracheal system Source: FlyBase
- zonula adherens assembly Source: FlyBase
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Ligand | ATP-binding, Metal-binding, Nucleotide-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-DME-2173791, TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition) R-DME-5218921, VEGFR2 mediated cell proliferation R-DME-9634635, Estrogen-stimulated signaling through PRKCZ |
SignaLinki | A1Z9X0 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:aPKCImported ORF Names:CG30475 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0261854, aPKC |
VEuPathDBi | VectorBase:FBgn0261854 |
Subcellular locationi
Plasma membrane
- Apicolateral cell membrane 6 Publications; Peripheral membrane protein 6 Publications
Other locations
- cell cortex 6 Publications
Note: Cytoplasmic at interphase but localizes to the apical cell cortex during mitosis.6 Publications
Plasma Membrane
- apical plasma membrane Source: FlyBase
- apicolateral plasma membrane Source: FlyBase
- subapical complex Source: FlyBase
Other locations
- apical cortex Source: FlyBase
- cell cortex Source: FlyBase
Keywords - Cellular componenti
Cell membrane, Cytoplasm, MembranePathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000430488 | 1 – 606 | Atypical protein kinase CAdd BLAST | 606 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | A1Z9X0 |
PRIDEi | A1Z9X0 |
PTM databases
iPTMneti | A1Z9X0 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | FBgn0261854, Expressed in wing disc (Drosophila) and 43 other tissues |
ExpressionAtlasi | A1Z9X0, baseline and differential |
Genevisiblei | A1Z9X0, DM |
Interactioni
Subunit structurei
Interacts with baz; the interaction is required for apical localization of aPKC in neuroblasts and epithelial cells.
Interacts with Dap160; the interaction promotes aPKC apical localization and kinase activity.
Interacts with and phosphorylates l2gl and yrt.
Interacts with crb and ref2P.
Forms a complex with baz, fz and Patj.
6 PublicationsBinary interactionsi
A1Z9X0
With | #Exp. | IntAct |
---|---|---|
baz [O96782] | 2 | EBI-160861,EBI-867941 |
baz [Q9VX75] | 3 | EBI-160861,EBI-2295779 |
crb [P10040] | 3 | EBI-160861,EBI-672928 |
par-6 [O97111] | 6 | EBI-160861,EBI-186645 |
Patj [Q9NB04] | 2 | EBI-160861,EBI-442573 |
GO - Molecular functioni
- myosin binding Source: FlyBase
Protein-protein interaction databases
BioGRIDi | 70851, 56 interactors |
IntActi | A1Z9X0, 10 interactors |
STRINGi | 7227.FBpp0302921 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 30 – 113 | PB1PROSITE-ProRule annotationAdd BLAST | 84 | |
Domaini | 264 – 532 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 269 | |
Domaini | 533 – 604 | AGC-kinase C-terminalPROSITE-ProRule annotationAdd BLAST | 72 |
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 145 – 195 | Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST | 51 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG0695, Eukaryota |
GeneTreei | ENSGT00940000169305 |
OMAi | LMSMEDC |
PhylomeDBi | A1Z9X0 |
Family and domain databases
CDDi | cd00029, C1, 1 hit cd06404, PB1_aPKC, 1 hit cd05588, STKc_aPKC, 1 hit |
InterProi | View protein in InterPro IPR000961, AGC-kinase_C IPR034659, Atypical_PKC IPR020454, DAG/PE-bd IPR011009, Kinase-like_dom_sf IPR034877, PB1_aPKC IPR000270, PB1_dom IPR002219, PE/DAG-bd IPR012233, PKC IPR017892, Pkinase_C IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00130, C1_1, 1 hit PF00564, PB1, 1 hit PF00069, Pkinase, 1 hit PF00433, Pkinase_C, 1 hit |
PIRSFi | PIRSF000554, PKC_zeta, 1 hit |
PRINTSi | PR00008, DAGPEDOMAIN |
SMARTi | View protein in SMART SM00109, C1, 1 hit SM00666, PB1, 1 hit SM00133, S_TK_X, 1 hit SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS51285, AGC_KINASE_CTER, 1 hit PS51745, PB1, 1 hit PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit PS00479, ZF_DAG_PE_1, 1 hit PS50081, ZF_DAG_PE_2, 1 hit |
s (5+)i Sequence
Sequence statusi: Complete.
This entry describes 5 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 5 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MQKMPSQILN DGSSVSLNSA SMNMANTPNS ITVKTAYNGQ IIITTINKNI
60 70 80 90 100
SYEELCYEIR NICRFPLDQP FTIKWVDEEN DPCTISTKME LDEAIRLYEM
110 120 130 140 150
NFDSQLVIHV FPNVPQAPGL SCDGEDRSIY RRGARRWRKL YRVNGHIFQA
160 170 180 190 200
KRFNRRAFCA YCQDRIWGLG RQGFKCIQCK LLVHKKCHKL VQKHCTDQPE
210 220 230 240 250
PLVKERAEES SDPIPVPLPP LPYEAMSGGA EACETHDHAH IVAPPPPEDP
260 270 280 290 300
LEPGTQRQYS LNDFELIRVI GRGSYAKVLM VELRRTRRIY AMKVIKKALV
310 320 330 340 350
TDDEDIDWVQ TEKHVFETAS NHPFLVGLHS CFQTPSRLFF VIEFVRGGDL
360 370 380 390 400
MYHMQRQRRL PEEHARFYAA EISLALNFLH EKGIIYRDLK LDNVLLDHEG
410 420 430 440 450
HIKLTDYGMC KEGIRPGDTT STFCGTPNYI APEILRGEDY GFSVDWWALG
460 470 480 490 500
VLLYEMLAGR SPFDLAGASE NPDQNTEDYL FQVILEKTIR IPRSLSVRAA
510 520 530 540 550
SVLKGFLNKN PADRLGCHRE SAFMDIVSHP FFKNMDWELL ERKQVTPPFK
560 570 580 590 600
PRLDSDRDLA NFPPEFTGEA VQLTPDDDHV IDNIDQSEFE GFEYVNPLLM
SLEDCV
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0B4KEQ8 | A0A0B4KEQ8_DROME | Protein kinase C | aPKC a-PKC, alphaPKCzeta, aPkc, apkc, aPKC zeta | 620 | Annotation score: | ||
A0A0B4KG15 | A0A0B4KG15_DROME | Protein kinase C | aPKC a-PKC, alphaPKCzeta, aPkc, apkc, aPKC zeta | 958 | Annotation score: | ||
A0A0B4JD78 | A0A0B4JD78_DROME | Protein kinase C | aPKC a-PKC, alphaPKCzeta, aPkc, apkc, aPKC zeta | 951 | Annotation score: | ||
A0A0B4KEV2 | A0A0B4KEV2_DROME | Protein kinase C | aPKC a-PKC, alphaPKCzeta, aPkc, apkc, aPKC zeta | 634 | Annotation score: | ||
A0A0B4KFQ6 | A0A0B4KFQ6_DROME | Protein kinase C | aPKC a-PKC, alphaPKCzeta, aPkc, apkc, aPKC zeta | 519 | Annotation score: | ||
A0A0B4JD26 | A0A0B4JD26_DROME | Atypical protein kinase C, isoform ... | aPKC a-PKC, alphaPKCzeta, aPkc, apkc, aPKC zeta | 511 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 16 | S → T in AAG01528 (PubMed:10995441).Curated | 1 | |
Sequence conflicti | 18 | N → S in AAG01528 (PubMed:10995441).Curated | 1 | |
Sequence conflicti | 101 | N → K in AAM48431 (PubMed:12537569).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_056764 | 1 – 103 | Missing in isoform F. 1 PublicationAdd BLAST | 103 | |
Alternative sequenceiVSP_056765 | 1 – 82 | Missing in isoform B and isoform E. 1 PublicationAdd BLAST | 82 | |
Alternative sequenceiVSP_056766 | 83 – 110 | CTIST…LVIHV → MIIWSGFCEAAQIYSTQTAT FMSGGASL in isoform B and isoform E. 1 PublicationAdd BLAST | 28 | |
Alternative sequenceiVSP_056767 | 104 – 110 | SQLVIHV → MGKLACL in isoform F. 1 Publication | 7 | |
Alternative sequenceiVSP_056768 | 540 – 578 | LERKQ…TPDDD → IAQKEVQPPYIPNIDTGDPY VTSNFDVQFTQEPAVLTPDD P in isoform B and isoform D. 1 PublicationAdd BLAST | 39 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF288482 mRNA Translation: AAG01528.1 AE013599 Genomic DNA Translation: AAF58177.2 AE013599 Genomic DNA Translation: ABI31090.1 AE013599 Genomic DNA Translation: ABI31091.1 AE013599 Genomic DNA Translation: ABI31092.1 AE013599 Genomic DNA Translation: ABI31093.1 AE013599 Genomic DNA Translation: ABI31094.1 AY118402 mRNA Translation: AAM48431.1 BT056247 mRNA Translation: ACL68694.1 |
RefSeqi | NP_001036541.1, NM_001043076.3 [A1Z9X0-1] NP_001036542.1, NM_001043077.3 [A1Z9X0-5] NP_001036543.1, NM_001043078.2 [A1Z9X0-4] NP_001036544.1, NM_001043079.2 [A1Z9X0-2] NP_001036545.1, NM_001043080.3 [A1Z9X0-3] NP_524892.2, NM_080153.5 [A1Z9X0-1] |
Genome annotation databases
EnsemblMetazoai | FBtr0303429; FBpp0292486; FBgn0261854 [A1Z9X0-2] FBtr0303430; FBpp0292487; FBgn0261854 [A1Z9X0-1] FBtr0303431; FBpp0292488; FBgn0261854 [A1Z9X0-3] FBtr0303432; FBpp0292489; FBgn0261854 [A1Z9X0-4] FBtr0303433; FBpp0292490; FBgn0261854 [A1Z9X0-5] FBtr0303434; FBpp0292491; FBgn0261854 [A1Z9X0-1] |
GeneIDi | 47594 |
KEGGi | dme:Dmel_CG42783 |
UCSCi | CG10261-RA, d. melanogaster [A1Z9X0-1] CG10261-RB, d. melanogaster CG10261-RD, d. melanogaster CG10261-RE, d. melanogaster CG10261-RF, d. melanogaster |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF288482 mRNA Translation: AAG01528.1 AE013599 Genomic DNA Translation: AAF58177.2 AE013599 Genomic DNA Translation: ABI31090.1 AE013599 Genomic DNA Translation: ABI31091.1 AE013599 Genomic DNA Translation: ABI31092.1 AE013599 Genomic DNA Translation: ABI31093.1 AE013599 Genomic DNA Translation: ABI31094.1 AY118402 mRNA Translation: AAM48431.1 BT056247 mRNA Translation: ACL68694.1 |
RefSeqi | NP_001036541.1, NM_001043076.3 [A1Z9X0-1] NP_001036542.1, NM_001043077.3 [A1Z9X0-5] NP_001036543.1, NM_001043078.2 [A1Z9X0-4] NP_001036544.1, NM_001043079.2 [A1Z9X0-2] NP_001036545.1, NM_001043080.3 [A1Z9X0-3] NP_524892.2, NM_080153.5 [A1Z9X0-1] |
3D structure databases
SMRi | A1Z9X0 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 70851, 56 interactors |
IntActi | A1Z9X0, 10 interactors |
STRINGi | 7227.FBpp0302921 |
PTM databases
iPTMneti | A1Z9X0 |
Proteomic databases
PaxDbi | A1Z9X0 |
PRIDEi | A1Z9X0 |
Genome annotation databases
EnsemblMetazoai | FBtr0303429; FBpp0292486; FBgn0261854 [A1Z9X0-2] FBtr0303430; FBpp0292487; FBgn0261854 [A1Z9X0-1] FBtr0303431; FBpp0292488; FBgn0261854 [A1Z9X0-3] FBtr0303432; FBpp0292489; FBgn0261854 [A1Z9X0-4] FBtr0303433; FBpp0292490; FBgn0261854 [A1Z9X0-5] FBtr0303434; FBpp0292491; FBgn0261854 [A1Z9X0-1] |
GeneIDi | 47594 |
KEGGi | dme:Dmel_CG42783 |
UCSCi | CG10261-RA, d. melanogaster [A1Z9X0-1] CG10261-RB, d. melanogaster CG10261-RD, d. melanogaster CG10261-RE, d. melanogaster CG10261-RF, d. melanogaster |
Organism-specific databases
CTDi | 47594 |
FlyBasei | FBgn0261854, aPKC |
VEuPathDBi | VectorBase:FBgn0261854 |
Phylogenomic databases
eggNOGi | KOG0695, Eukaryota |
GeneTreei | ENSGT00940000169305 |
OMAi | LMSMEDC |
PhylomeDBi | A1Z9X0 |
Enzyme and pathway databases
Reactomei | R-DME-2173791, TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition) R-DME-5218921, VEGFR2 mediated cell proliferation R-DME-9634635, Estrogen-stimulated signaling through PRKCZ |
SignaLinki | A1Z9X0 |
Miscellaneous databases
BioGRID-ORCSi | 47594, 0 hits in 3 CRISPR screens |
GenomeRNAii | 47594 |
PROi | PR:A1Z9X0 |
Gene expression databases
Bgeei | FBgn0261854, Expressed in wing disc (Drosophila) and 43 other tissues |
ExpressionAtlasi | A1Z9X0, baseline and differential |
Genevisiblei | A1Z9X0, DM |
Family and domain databases
CDDi | cd00029, C1, 1 hit cd06404, PB1_aPKC, 1 hit cd05588, STKc_aPKC, 1 hit |
InterProi | View protein in InterPro IPR000961, AGC-kinase_C IPR034659, Atypical_PKC IPR020454, DAG/PE-bd IPR011009, Kinase-like_dom_sf IPR034877, PB1_aPKC IPR000270, PB1_dom IPR002219, PE/DAG-bd IPR012233, PKC IPR017892, Pkinase_C IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00130, C1_1, 1 hit PF00564, PB1, 1 hit PF00069, Pkinase, 1 hit PF00433, Pkinase_C, 1 hit |
PIRSFi | PIRSF000554, PKC_zeta, 1 hit |
PRINTSi | PR00008, DAGPEDOMAIN |
SMARTi | View protein in SMART SM00109, C1, 1 hit SM00666, PB1, 1 hit SM00133, S_TK_X, 1 hit SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS51285, AGC_KINASE_CTER, 1 hit PS51745, PB1, 1 hit PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit PS00479, ZF_DAG_PE_1, 1 hit PS50081, ZF_DAG_PE_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | APKC_DROME | |
Accessioni | A1Z9X0Primary (citable) accession number: A1Z9X0 Secondary accession number(s): Q0E971 Q9GSZ3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 2014 |
Last sequence update: | February 6, 2007 | |
Last modified: | February 23, 2022 | |
This is version 148 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - SIMILARITY comments
Index of protein domains and families