UniProtKB - A1Z6E0 (GUS_DROME)
Protein
Protein gustavus
Gene
gus
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Involved in the localization of vas to the posterior pole of the oocyte. Required maternally in the germ line for efficient primordial germ cell formation.2 Publications
GO - Biological processi
- cuticle pattern formation Source: UniProtKB
- dorsal appendage formation Source: FlyBase
- germ cell development Source: UniProtKB
- intracellular signal transduction Source: InterPro
- oocyte anterior/posterior axis specification Source: FlyBase
- pole cell migration Source: UniProtKB
- pole plasm assembly Source: UniProtKB
- positive regulation of protein catabolic process Source: FlyBase
- proteasome-mediated ubiquitin-dependent protein catabolic process Source: GO_Central
- protein localization Source: UniProtKB
- ubiquitin-dependent protein catabolic process Source: GO_Central
- wing disc morphogenesis Source: UniProtKB
Keywordsi
Molecular function | Developmental protein |
Enzyme and pathway databases
Reactomei | R-DME-8951664, Neddylation R-DME-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Names & Taxonomyi
Protein namesi | Recommended name: Protein gustavusImported |
Gene namesi | Name:gus ORF Names:CG2944 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0026238, gus |
Subcellular locationi
Nucleus
- Nucleus 2 Publications
Other locations
- Cytoplasm 2 Publications
Note: Component of the cytoplasmic ribonucleoprotein (RNP) bodies which concentrate at the perinuclear region of the nurse cell and in punctate aggregates throughout the cytoplasm. Some cortical enrichment of these aggregates is also observed. At stage 7, accumulates in the anterior cytoplasm of the oocyte, whereas during stage 10 accumulates at the posterior pole. This posterior distribution is not maintained in later developmental stages.2 Publications
Nucleus
- elongin complex Source: UniProtKB
- nucleus Source: UniProtKB
Other locations
- cell cortex Source: UniProtKB
- Cul5-RING ubiquitin ligase complex Source: FlyBase
- cytoplasm Source: UniProtKB
- perinuclear region of cytoplasm Source: UniProtKB
- pole plasm Source: UniProtKB
- SCF ubiquitin ligase complex Source: GO_Central
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Disruption phenotypei
Homozygous females produce defective eggs, which desiccate and collapse soon after egg laying, or embryos, which hatch very rarely. Mutants exhibit wing morphology defects, including blistering, formation of ectopic vein material, and loss of material at the margin.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 131 | Y → A: Does not affect binding to Elongin BC complex. Abolishes interaction with vas. 1 Publication | 1 | |
Mutagenesisi | 147 | G → Y: Does not affect binding to Elongin BC complex. Abolishes interaction with vas. 1 Publication | 1 | |
Mutagenesisi | 148 | R → W: Does not affect binding to Elongin BC complex. Abolishes interaction with vas. 1 Publication | 1 | |
Mutagenesisi | 187 | E → A: Does not affect binding to Elongin BC complex or vas. 1 Publication | 1 | |
Mutagenesisi | 204 | R → S: Does not affect binding to Elongin BC complex or vas. 1 Publication | 1 | |
Mutagenesisi | 219 | W → L: Does not affect binding to Elongin BC complex. Decreases interaction with vas. 3 Publications | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000422158 | 1 – 279 | Protein gustavusAdd BLAST | 279 |
Proteomic databases
PaxDbi | A1Z6E0 |
Expressioni
Tissue specificityi
Expressed in ovaries, primarily in nurse cells and oocytes (at protein level).2 Publications
Developmental stagei
Expressed during oogenesis, in early embryos (0 to 4 hours) and in fertile adult females (at protein level).2 Publications
Gene expression databases
Bgeei | FBgn0026238, Expressed in embryo and 40 other tissues |
ExpressionAtlasi | A1Z6E0, baseline and differential |
Genevisiblei | A1Z6E0, DM |
Interactioni
Subunit structurei
Interacts (via B30.2/SPRY domain) with vas; this interaction may be necessary for the transport of vas to the posterior pole of the oocyte.
Interacts with Cul-5. May associate with the Elongin BC complex composed of Elongin-B and Elongin-C.
4 PublicationsBinary interactionsi
Hide detailsProtein-protein interaction databases
BioGRIDi | 61396, 50 interactors |
IntActi | A1Z6E0, 35 interactors |
MINTi | A1Z6E0 |
STRINGi | 7227.FBpp0301582 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | A1Z6E0 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | A1Z6E0 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 36 – 233 | B30.2/SPRYPROSITE-ProRule annotationAdd BLAST | 198 | |
Domaini | 234 – 279 | SOCS boxPROSITE-ProRule annotationAdd BLAST | 46 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 236 – 279 | Involved in binding to the Elongin BC complex1 PublicationAdd BLAST | 44 |
Sequence similaritiesi
Belongs to the SPSB family.Curated
Phylogenomic databases
eggNOGi | KOG3953, Eukaryota |
GeneTreei | ENSGT01010000222472 |
HOGENOMi | CLU_046756_0_1_1 |
InParanoidi | A1Z6E0 |
OMAi | DLCRRCI |
OrthoDBi | 938259at2759 |
Family and domain databases
Gene3Di | 2.60.120.920, 1 hit |
InterProi | View protein in InterPro IPR001870, B30.2/SPRY IPR043136, B30.2/SPRY_sf IPR013320, ConA-like_dom_sf IPR001496, SOCS_box IPR036036, SOCS_box-like_dom_sf IPR003877, SPRY_dom |
Pfami | View protein in Pfam PF07525, SOCS_box, 1 hit PF00622, SPRY, 1 hit |
SMARTi | View protein in SMART SM00969, SOCS_box, 1 hit SM00449, SPRY, 1 hit |
SUPFAMi | SSF158235, SSF158235, 1 hit SSF49899, SSF49899, 1 hit |
PROSITEi | View protein in PROSITE PS50188, B302_SPRY, 1 hit PS50225, SOCS, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
A1Z6E0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGQKISGGVK TVSRNDSQST FKPIIPRELQ ADFVKPARID ILLDMPPASR
60 70 80 90 100
DLQLKHSWNS EDRSLNIFVK EDDKLTFHRH PVAQSTDCIR GKVGLTKGLH
110 120 130 140 150
IWEIYWPTRQ RGTHAVVGVC TADAPLHSVG YQSLVGSTEQ SWGWDLGRNK
160 170 180 190 200
LYHDSKNCAG VTYPAILKND EAFLVPDKFL VALDMDEGTL SFIVDQQYLG
210 220 230 240 250
IAFRGLRGKK LYPIVSAVWG HCEITMRYIG GLDPEPLPLM DLCRRTIRQK
260 270
IGRTNLEEHI QQLQLPLSMK TYLLYKNRR
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0B4K6X3 | A0A0B4K6X3_DROME | Gustavus, isoform G | gus A11, CG2946, Dmel\CG2944, dmSSB4, GUS | 279 | Annotation score: |
Sequence cautioni
The sequence AAM50263 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
The sequence ACU12389 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
The sequence ACX32987 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
The sequence ADZ74173 differs from that shown. Reason: Frameshift.Curated
The sequence ADZ74173 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE013599 Genomic DNA Translation: AAF57346.2 AE013599 Genomic DNA Translation: AAF57347.3 AE013599 Genomic DNA Translation: AAG22337.2 AE013599 Genomic DNA Translation: AAM68372.1 AE013599 Genomic DNA Translation: AAM68373.2 AE013599 Genomic DNA Translation: AAM68374.2 AY119609 mRNA Translation: AAM50263.1 Different initiation. BT089043 mRNA Translation: ACU12389.1 Different initiation. BT099916 mRNA Translation: ACX32987.1 Different initiation. BT126146 mRNA Translation: ADZ74173.1 Sequence problems. |
RefSeqi | NP_001246140.1, NM_001259211.2 NP_610158.3, NM_136314.4 NP_724398.2, NM_165418.3 NP_724399.2, NM_165419.3 NP_724400.2, NM_165420.3 NP_724401.2, NM_165421.3 NP_724402.2, NM_165422.3 |
Genome annotation databases
EnsemblMetazoai | FBtr0309846; FBpp0301580; FBgn0026238 FBtr0309847; FBpp0301581; FBgn0026238 FBtr0309848; FBpp0301582; FBgn0026238 FBtr0309849; FBpp0301583; FBgn0026238 FBtr0309850; FBpp0301584; FBgn0026238 FBtr0309851; FBpp0301585; FBgn0026238 FBtr0309852; FBpp0301586; FBgn0026238 |
GeneIDi | 35478 |
KEGGi | dme:Dmel_CG2944 |
UCSCi | CG2944-RA, d. melanogaster CG2944-RB, d. melanogaster CG2944-RD, d. melanogaster CG2944-RE, d. melanogaster CG2944-RF, d. melanogaster |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE013599 Genomic DNA Translation: AAF57346.2 AE013599 Genomic DNA Translation: AAF57347.3 AE013599 Genomic DNA Translation: AAG22337.2 AE013599 Genomic DNA Translation: AAM68372.1 AE013599 Genomic DNA Translation: AAM68373.2 AE013599 Genomic DNA Translation: AAM68374.2 AY119609 mRNA Translation: AAM50263.1 Different initiation. BT089043 mRNA Translation: ACU12389.1 Different initiation. BT099916 mRNA Translation: ACX32987.1 Different initiation. BT126146 mRNA Translation: ADZ74173.1 Sequence problems. |
RefSeqi | NP_001246140.1, NM_001259211.2 NP_610158.3, NM_136314.4 NP_724398.2, NM_165418.3 NP_724399.2, NM_165419.3 NP_724400.2, NM_165420.3 NP_724401.2, NM_165421.3 NP_724402.2, NM_165422.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2FNJ | X-ray | 1.80 | A | 27-251 | [»] | |
2IHS | X-ray | 2.20 | A/B | 27-232 | [»] | |
SMRi | A1Z6E0 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 61396, 50 interactors |
IntActi | A1Z6E0, 35 interactors |
MINTi | A1Z6E0 |
STRINGi | 7227.FBpp0301582 |
Proteomic databases
PaxDbi | A1Z6E0 |
Genome annotation databases
EnsemblMetazoai | FBtr0309846; FBpp0301580; FBgn0026238 FBtr0309847; FBpp0301581; FBgn0026238 FBtr0309848; FBpp0301582; FBgn0026238 FBtr0309849; FBpp0301583; FBgn0026238 FBtr0309850; FBpp0301584; FBgn0026238 FBtr0309851; FBpp0301585; FBgn0026238 FBtr0309852; FBpp0301586; FBgn0026238 |
GeneIDi | 35478 |
KEGGi | dme:Dmel_CG2944 |
UCSCi | CG2944-RA, d. melanogaster CG2944-RB, d. melanogaster CG2944-RD, d. melanogaster CG2944-RE, d. melanogaster CG2944-RF, d. melanogaster |
Organism-specific databases
CTDi | 35478 |
FlyBasei | FBgn0026238, gus |
Phylogenomic databases
eggNOGi | KOG3953, Eukaryota |
GeneTreei | ENSGT01010000222472 |
HOGENOMi | CLU_046756_0_1_1 |
InParanoidi | A1Z6E0 |
OMAi | DLCRRCI |
OrthoDBi | 938259at2759 |
Enzyme and pathway databases
Reactomei | R-DME-8951664, Neddylation R-DME-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Miscellaneous databases
BioGRID-ORCSi | 35478, 0 hits in 3 CRISPR screens |
EvolutionaryTracei | A1Z6E0 |
GenomeRNAii | 35478 |
PROi | PR:A1Z6E0 |
Gene expression databases
Bgeei | FBgn0026238, Expressed in embryo and 40 other tissues |
ExpressionAtlasi | A1Z6E0, baseline and differential |
Genevisiblei | A1Z6E0, DM |
Family and domain databases
Gene3Di | 2.60.120.920, 1 hit |
InterProi | View protein in InterPro IPR001870, B30.2/SPRY IPR043136, B30.2/SPRY_sf IPR013320, ConA-like_dom_sf IPR001496, SOCS_box IPR036036, SOCS_box-like_dom_sf IPR003877, SPRY_dom |
Pfami | View protein in Pfam PF07525, SOCS_box, 1 hit PF00622, SPRY, 1 hit |
SMARTi | View protein in SMART SM00969, SOCS_box, 1 hit SM00449, SPRY, 1 hit |
SUPFAMi | SSF158235, SSF158235, 1 hit SSF49899, SSF49899, 1 hit |
PROSITEi | View protein in PROSITE PS50188, B302_SPRY, 1 hit PS50225, SOCS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GUS_DROME | |
Accessioni | A1Z6E0Primary (citable) accession number: A1Z6E0 Secondary accession number(s): A1Z6D7 Q7JUY1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 2013 |
Last sequence update: | March 16, 2016 | |
Last modified: | December 2, 2020 | |
This is version 117 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families