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Protein

Fumarate hydratase class II

Gene

fumC

Organism
Mycobacterium sp. (strain KMS)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate.UniRule annotation

Miscellaneous

There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors.UniRule annotation

Catalytic activityi

(S)-malate = fumarate + H2O.UniRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from fumarate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Fumarate hydratase class II (fumC)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from fumarate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei189SubstrateUniRule annotation1
Active sitei190Proton donor/acceptorUniRule annotation1
Active sitei321UniRule annotation1
Binding sitei322SubstrateUniRule annotation1
Sitei334Important for catalytic activityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyaseUniRule annotationSAAS annotationImported
Biological processTricarboxylic acid cycleUniRule annotation

Enzyme and pathway databases

UniPathwayi
UPA00223;UER01007

Names & Taxonomyi

Protein namesi
Recommended name:
Fumarate hydratase class IIUniRule annotation (EC:4.2.1.2UniRule annotation)
Short name:
Fumarase CUniRule annotation
Alternative name(s):
Aerobic fumaraseUniRule annotation
Iron-independent fumaraseUniRule annotation
Gene namesi
Name:fumCUniRule annotation
Ordered Locus Names:Mkms_4197Imported
OrganismiMycobacterium sp. (strain KMS)Imported
Taxonomic identifieri189918 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000638 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA1UKN1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini21 – 345Lyase_1InterPro annotationAdd BLAST325
Domaini411 – 467FumaraseC_CInterPro annotationAdd BLAST57

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni107 – 109Substrate bindingUniRule annotation3
Regioni131 – 134Substrate binding (B site)UniRule annotation4
Regioni141 – 143Substrate bindingUniRule annotation3
Regioni327 – 329Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000061737
KOiK01679
OMAiRIEYDTF
OrthoDBiPOG091H01XG

Family and domain databases

CDDicd01362 Fumarase_classII, 1 hit
Gene3Di1.10.275.10, 1 hit
HAMAPiMF_00743 FumaraseC, 1 hit
InterProiView protein in InterPro
IPR005677 Fum_hydII
IPR024083 Fumarase/histidase_N
IPR018951 Fumarase_C_C
IPR020557 Fumarate_lyase_CS
IPR000362 Fumarate_lyase_fam
IPR022761 Fumarate_lyase_N
IPR008948 L-Aspartase-like
PANTHERiPTHR11444 PTHR11444, 1 hit
PfamiView protein in Pfam
PF10415 FumaraseC_C, 1 hit
PF00206 Lyase_1, 1 hit
PRINTSiPR00149 FUMRATELYASE
SUPFAMiSSF48557 SSF48557, 1 hit
PROSITEiView protein in PROSITE
PS00163 FUMARATE_LYASES, 1 hit

Sequencei

Sequence statusi: Complete.

A1UKN1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTADSAVDQN TEYRIEHDTM GEVRVPKDAL WRAQTQRAVE NFPISGRGLE
60 70 80 90 100
RTQIRALGLL KGACAQVNKD LGLLAPEKAD AIIAAAAEIA DGRHDDQFPI
110 120 130 140 150
DVFQTGSGTS SNMNSNEVIA SIAAANGVTV HPNDDVNMSQ SSNDTFPTAT
160 170 180 190 200
HIAATEAAVR HLIPALEVLH ESLAGKARQW RTVVKSGRTH LMDAVPVTLG
210 220 230 240 250
QEFGGYARQI EAGIERVKAT LPRLGELAIG GTAVGTGLNA PDGFGAKVVE
260 270 280 290 300
VLVDQTGLGE LTTAKDSFEA QAARDGLVEA SGALRVIAVS LTKIANDIRW
310 320 330 340 350
MGSGPLTGLA EIQLPDLQPG SSIMPGKVNP VLPEAVTQVA AQVVGNDAAV
360 370 380 390 400
AWGGAAGAFE LNVYIPMMAR NLLESFKLLT NVSRLFAERC IDGLVANEEH
410 420 430 440 450
MREQAESSPS IVTPLNSAIG YEEAAKVAKE ALKEKKTIRQ TVIDRGLIGD
460 470
KLSEEELDRR LDVLAMAKVK QGK
Length:473
Mass (Da):50,214
Last modified:February 6, 2007 - v1
Checksum:iC4E3CD671658746A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000518 Genomic DNA Translation: ABL93389.1
RefSeqiWP_011561500.1, NC_008705.1

Genome annotation databases

EnsemblBacteriaiABL93389; ABL93389; Mkms_4197
GeneIDi32421326
KEGGimkm:Mkms_4197

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000518 Genomic DNA Translation: ABL93389.1
RefSeqiWP_011561500.1, NC_008705.1

3D structure databases

ProteinModelPortaliA1UKN1
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABL93389; ABL93389; Mkms_4197
GeneIDi32421326
KEGGimkm:Mkms_4197

Phylogenomic databases

HOGENOMiHOG000061737
KOiK01679
OMAiRIEYDTF
OrthoDBiPOG091H01XG

Enzyme and pathway databases

UniPathwayi
UPA00223;UER01007

Family and domain databases

CDDicd01362 Fumarase_classII, 1 hit
Gene3Di1.10.275.10, 1 hit
HAMAPiMF_00743 FumaraseC, 1 hit
InterProiView protein in InterPro
IPR005677 Fum_hydII
IPR024083 Fumarase/histidase_N
IPR018951 Fumarase_C_C
IPR020557 Fumarate_lyase_CS
IPR000362 Fumarate_lyase_fam
IPR022761 Fumarate_lyase_N
IPR008948 L-Aspartase-like
PANTHERiPTHR11444 PTHR11444, 1 hit
PfamiView protein in Pfam
PF10415 FumaraseC_C, 1 hit
PF00206 Lyase_1, 1 hit
PRINTSiPR00149 FUMRATELYASE
SUPFAMiSSF48557 SSF48557, 1 hit
PROSITEiView protein in PROSITE
PS00163 FUMARATE_LYASES, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiA1UKN1_MYCSK
AccessioniPrimary (citable) accession number: A1UKN1
Entry historyiIntegrated into UniProtKB/TrEMBL: February 6, 2007
Last sequence update: February 6, 2007
Last modified: November 7, 2018
This is version 72 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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