UniProtKB - A1L259 (URAD_DANRE)
Protein
2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase
Gene
urad
Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Functioni
Catalyzes the stereoselective decarboxylation of 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline (OHCU) to (S)-allantoin.1 Publication
Catalytic activityi
- EC:4.1.1.97
: urate degradation Pathwayi
This protein is involved in step 3 of the subpathway that synthesizes (S)-allantoin from urate.Proteins known to be involved in the 3 steps of the subpathway in this organism are:
- Uricase (uox), Uricase (uox), Uricase (uox)
- 5-hydroxyisourate hydrolase (urah)
- 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase (urad)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-allantoin from urate, the pathway urate degradation and in Purine metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 67 | Proton donorBy similarity | 1 | |
Binding sitei | 68 | Substrate; via carbonyl oxygen | 1 |
GO - Molecular functioni
- 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase activity Source: ZFIN
- carboxy-lyase activity Source: GO_Central
GO - Biological processi
- allantoin biosynthetic process Source: InterPro
- purine nucleobase metabolic process Source: UniProtKB-KW
- urate catabolic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Decarboxylase, Lyase |
Biological process | Purine metabolism |
Enzyme and pathway databases
BRENDAi | 4.1.1.97, 928 |
UniPathwayi | UPA00394;UER00652 |
Names & Taxonomyi
Protein namesi | Recommended name: 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase (EC:4.1.1.97)Short name: OHCU decarboxylase Alternative name(s): Parahox neighbor Ureidoimidazoline (2-oxo-4-hydroxy-4-carboxy-5-) decarboxylase |
Gene namesi | Name:urad Synonyms:prhoxnb ORF Names:zgc:158663 |
Organismi | Danio rerio (Zebrafish) (Brachydanio rerio) |
Taxonomic identifieri | 7955 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Danionidae › Danioninae › Danio |
Proteomesi |
|
Organism-specific databases
ZFINi | ZDB-GENE-070112-472, urad |
Subcellular locationi
Peroxisome
- Peroxisome Curated
Peroxisome
- peroxisome Source: UniProtKB-SubCell
Keywords - Cellular componenti
PeroxisomePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 67 | H → N: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 87 | E → Q: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 161 | R → Q: Loss of activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000315243 | 1 – 174 | 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylaseAdd BLAST | 174 |
Proteomic databases
PaxDbi | A1L259 |
PeptideAtlasi | A1L259 |
Expressioni
Gene expression databases
Bgeei | ENSDARG00000071579, Expressed in liver and 10 other tissues |
Interactioni
Subunit structurei
Homodimer.
1 PublicationProtein-protein interaction databases
STRINGi | 7955.ENSDARP00000096695 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | A1L259 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | A1L259 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 84 – 88 | Substrate binding | 5 | |
Regioni | 119 – 123 | Substrate binding | 5 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 172 – 174 | Microbody targeting signalSequence analysis | 3 |
Sequence similaritiesi
Belongs to the OHCU decarboxylase family.Curated
Phylogenomic databases
eggNOGi | KOG0848, Eukaryota |
GeneTreei | ENSGT00390000002395 |
HOGENOMi | CLU_092522_1_0_1 |
InParanoidi | A1L259 |
OMAi | KICHLRL |
OrthoDBi | 1547390at2759 |
PhylomeDBi | A1L259 |
TreeFami | TF323276 |
Family and domain databases
Gene3Di | 1.10.3330.10, 1 hit |
InterProi | View protein in InterPro IPR018020, OHCU_decarboxylase IPR017580, OHCU_decarboxylase-1 IPR036778, OHCU_decarboxylase_sf |
Pfami | View protein in Pfam PF09349, OHCU_decarbox, 1 hit |
SUPFAMi | SSF158694, SSF158694, 1 hit |
TIGRFAMsi | TIGR03164, UHCUDC, 1 hit |
i Sequence
Sequence statusi: Complete.
A1L259-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDINVVNALA YEDFVKLFGN VVEKCPLISA AIWSYRPFKD LADIEARISE
60 70 80 90 100
FIHSLPDSGK EGILRCHPDL AGRDLQSGTL TPESQEEQSQ AGMTTLDSAE
110 120 130 140 150
IVHMYRLNSE YKERFGFPFV ICARLNNKAD IVRQLSERLK NRRTAELECA
160 170
IEEVKKICSL RLHSIVLSDI QTKL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | EF197726 mRNA Translation: ABP04232.1 BC129362 mRNA Translation: AAI29363.1 |
RefSeqi | NP_001073641.1, NM_001080172.1 |
Genome annotation databases
Ensembli | ENSDART00000105917; ENSDARP00000096695; ENSDARG00000071579 ENSDART00000184990; ENSDARP00000148135; ENSDARG00000115541 |
GeneIDi | 557735 |
KEGGi | dre:557735 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | EF197726 mRNA Translation: ABP04232.1 BC129362 mRNA Translation: AAI29363.1 |
RefSeqi | NP_001073641.1, NM_001080172.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2O70 | X-ray | 1.80 | A/B/C/D/E/F | 1-174 | [»] | |
2O73 | X-ray | 1.80 | A/B/C/D/E/F | 1-174 | [»] | |
2O74 | X-ray | 1.80 | A/B/C/D/E/F | 1-174 | [»] | |
SMRi | A1L259 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 7955.ENSDARP00000096695 |
Proteomic databases
PaxDbi | A1L259 |
PeptideAtlasi | A1L259 |
Genome annotation databases
Ensembli | ENSDART00000105917; ENSDARP00000096695; ENSDARG00000071579 ENSDART00000184990; ENSDARP00000148135; ENSDARG00000115541 |
GeneIDi | 557735 |
KEGGi | dre:557735 |
Organism-specific databases
CTDi | 646625 |
ZFINi | ZDB-GENE-070112-472, urad |
Phylogenomic databases
eggNOGi | KOG0848, Eukaryota |
GeneTreei | ENSGT00390000002395 |
HOGENOMi | CLU_092522_1_0_1 |
InParanoidi | A1L259 |
OMAi | KICHLRL |
OrthoDBi | 1547390at2759 |
PhylomeDBi | A1L259 |
TreeFami | TF323276 |
Enzyme and pathway databases
UniPathwayi | UPA00394;UER00652 |
BRENDAi | 4.1.1.97, 928 |
Miscellaneous databases
EvolutionaryTracei | A1L259 |
PROi | PR:A1L259 |
Gene expression databases
Bgeei | ENSDARG00000071579, Expressed in liver and 10 other tissues |
Family and domain databases
Gene3Di | 1.10.3330.10, 1 hit |
InterProi | View protein in InterPro IPR018020, OHCU_decarboxylase IPR017580, OHCU_decarboxylase-1 IPR036778, OHCU_decarboxylase_sf |
Pfami | View protein in Pfam PF09349, OHCU_decarbox, 1 hit |
SUPFAMi | SSF158694, SSF158694, 1 hit |
TIGRFAMsi | TIGR03164, UHCUDC, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | URAD_DANRE | |
Accessioni | A1L259Primary (citable) accession number: A1L259 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 15, 2008 |
Last sequence update: | February 6, 2007 | |
Last modified: | December 2, 2020 | |
This is version 90 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families