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Protein

2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase

Gene

urad

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the stereoselective decarboxylation of 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline (OHCU) to (S)-allantoin.1 Publication

Catalytic activityi

5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate = (S)-allantoin + CO2.

Pathwayi: urate degradation

This protein is involved in step 3 of the subpathway that synthesizes (S)-allantoin from urate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Uricase (uox)
  2. 5-hydroxyisourate hydrolase (urah)
  3. 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase (urad)
This subpathway is part of the pathway urate degradation, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-allantoin from urate, the pathway urate degradation and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei67Proton donorBy similarity1
Binding sitei68Substrate; via carbonyl oxygen1

GO - Molecular functioni

  • 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase activity Source: ZFIN
  • carboxy-lyase activity Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionDecarboxylase, Lyase
Biological processPurine metabolism

Enzyme and pathway databases

BRENDAi4.1.1.97 928
UniPathwayi
UPA00394;UER00652

Names & Taxonomyi

Protein namesi
Recommended name:
2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase (EC:4.1.1.97)
Short name:
OHCU decarboxylase
Alternative name(s):
Parahox neighbor
Ureidoimidazoline (2-oxo-4-hydroxy-4-carboxy-5-) decarboxylase
Gene namesi
Name:urad
Synonyms:prhoxnb
ORF Names:zgc:158663
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 24

Organism-specific databases

ZFINiZDB-GENE-070112-472 urad

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Peroxisome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi67H → N: Loss of activity. 1 Publication1
Mutagenesisi87E → Q: Loss of activity. 1 Publication1
Mutagenesisi161R → Q: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003152431 – 1742-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylaseAdd BLAST174

Proteomic databases

PaxDbiA1L259
PeptideAtlasiA1L259

Expressioni

Gene expression databases

BgeeiENSDARG00000071579 Expressed in 9 organ(s), highest expression level in liver

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi7955.ENSDARP00000096695

Structurei

Secondary structure

1174
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliA1L259
SMRiA1L259
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiA1L259

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni84 – 88Substrate binding5
Regioni119 – 123Substrate binding5

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi172 – 174Microbody targeting signalSequence analysis3

Sequence similaritiesi

Belongs to the OHCU decarboxylase family.Curated

Phylogenomic databases

eggNOGiENOG410IX4N Eukaryota
COG3195 LUCA
GeneTreeiENSGT00390000002395
HOGENOMiHOG000251764
HOVERGENiHBG108651
InParanoidiA1L259
KOiK13485
OMAiHSPFIAE
OrthoDBiEOG091G0RLD
PhylomeDBiA1L259
TreeFamiTF323276

Family and domain databases

Gene3Di1.10.3330.10, 1 hit
InterProiView protein in InterPro
IPR018020 OHCU_decarboxylase
IPR017580 OHCU_decarboxylase-1
IPR036778 OHCU_decarboxylase_sf
PfamiView protein in Pfam
PF09349 OHCU_decarbox, 1 hit
SUPFAMiSSF158694 SSF158694, 1 hit
TIGRFAMsiTIGR03164 UHCUDC, 1 hit

Sequencei

Sequence statusi: Complete.

A1L259-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDINVVNALA YEDFVKLFGN VVEKCPLISA AIWSYRPFKD LADIEARISE
60 70 80 90 100
FIHSLPDSGK EGILRCHPDL AGRDLQSGTL TPESQEEQSQ AGMTTLDSAE
110 120 130 140 150
IVHMYRLNSE YKERFGFPFV ICARLNNKAD IVRQLSERLK NRRTAELECA
160 170
IEEVKKICSL RLHSIVLSDI QTKL
Length:174
Mass (Da):19,743
Last modified:February 6, 2007 - v1
Checksum:i1B408D09F29FF4D7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF197726 mRNA Translation: ABP04232.1
BC129362 mRNA Translation: AAI29363.1
RefSeqiNP_001073641.1, NM_001080172.1
UniGeneiDr.87169

Genome annotation databases

EnsembliENSDART00000105917; ENSDARP00000096695; ENSDARG00000071579
ENSDART00000184990; ENSDARP00000148135; ENSDARG00000115541
GeneIDi557735
KEGGidre:557735

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF197726 mRNA Translation: ABP04232.1
BC129362 mRNA Translation: AAI29363.1
RefSeqiNP_001073641.1, NM_001080172.1
UniGeneiDr.87169

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2O70X-ray1.80A/B/C/D/E/F1-174[»]
2O73X-ray1.80A/B/C/D/E/F1-174[»]
2O74X-ray1.80A/B/C/D/E/F1-174[»]
ProteinModelPortaliA1L259
SMRiA1L259
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000096695

Proteomic databases

PaxDbiA1L259
PeptideAtlasiA1L259

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000105917; ENSDARP00000096695; ENSDARG00000071579
ENSDART00000184990; ENSDARP00000148135; ENSDARG00000115541
GeneIDi557735
KEGGidre:557735

Organism-specific databases

CTDi646625
ZFINiZDB-GENE-070112-472 urad

Phylogenomic databases

eggNOGiENOG410IX4N Eukaryota
COG3195 LUCA
GeneTreeiENSGT00390000002395
HOGENOMiHOG000251764
HOVERGENiHBG108651
InParanoidiA1L259
KOiK13485
OMAiHSPFIAE
OrthoDBiEOG091G0RLD
PhylomeDBiA1L259
TreeFamiTF323276

Enzyme and pathway databases

UniPathwayi
UPA00394;UER00652

BRENDAi4.1.1.97 928

Miscellaneous databases

EvolutionaryTraceiA1L259
PROiPR:A1L259

Gene expression databases

BgeeiENSDARG00000071579 Expressed in 9 organ(s), highest expression level in liver

Family and domain databases

Gene3Di1.10.3330.10, 1 hit
InterProiView protein in InterPro
IPR018020 OHCU_decarboxylase
IPR017580 OHCU_decarboxylase-1
IPR036778 OHCU_decarboxylase_sf
PfamiView protein in Pfam
PF09349 OHCU_decarbox, 1 hit
SUPFAMiSSF158694 SSF158694, 1 hit
TIGRFAMsiTIGR03164 UHCUDC, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiURAD_DANRE
AccessioniPrimary (citable) accession number: A1L259
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 6, 2007
Last modified: November 7, 2018
This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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