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UniProtKB - A1KQG0 (PHAS_MYCBP)
Protein
Phthioceranic/hydroxyphthioceranic acid synthase
Gene
pks2
Organism
Mycobacterium bovis (strain BCG / Pasteur 1173P2)
Status
Functioni
Miscellaneous
In strain BCG, the sulfolipid-1 (SL-1) is not synthesized.
Catalytic activityi
- 7 (S)-methylmalonyl-CoA + 21 H+ + hexadecanoyl-[(hydroxy)phthioceranic acid synthase] + 14 NADPH = C37-phthioceranyl-[(hydroxy)phthioceranic acid synthase] + 7 CO2 + 7 CoA + 7 H2O + 14 NADP+By similarityEC:2.3.1.287By similarity
- 8 (S)-methylmalonyl-CoA + 24 H+ + hexadecanoyl-[(hydroxy)phthioceranic acid synthase] + 16 NADPH = C40-phthioceranyl-[(hydroxy)phthioceranic acid synthase] + 8 CO2 + 8 CoA + 8 H2O + 16 NADP+By similarityEC:2.3.1.287By similarity
Cofactori
pantetheine 4'-phosphateBy similarityNote: Binds 1 phosphopantetheine covalently.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 196 | Acyl-thioester intermediate; for beta-ketoacyl synthase activityPROSITE-ProRule annotation | 1 | |
Active sitei | 641 | Acyl-ester intermediate; for acyltransferase activityBy similarity | 1 | |
Active sitei | 947 | Proton acceptor; for dehydratase activityBy similarity | 1 | |
Active sitei | 1115 | Proton donor; for dehydratase activityBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 1780 – 1783 | NADPBy similarity | 4 | |
Nucleotide bindingi | 1803 – 1806 | NADPBy similarity | 4 | |
Nucleotide bindingi | 1831 – 1832 | NADPBy similarity | 2 | |
Nucleotide bindingi | 1904 – 1905 | NADPBy similarity | 2 |
GO - Molecular functioni
- 3-oxoacyl-[acyl-carrier-protein] synthase activity Source: InterPro
- oxidoreductase activity Source: InterPro
- phosphopantetheine binding Source: InterPro
GO - Biological processi
- fatty acid biosynthetic process Source: UniProtKB-KW
Keywordsi
Molecular function | Acyltransferase, Multifunctional enzyme, Transferase |
Biological process | Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism |
Ligand | NADP |
Names & Taxonomyi
Protein namesi | Recommended name: Phthioceranic/hydroxyphthioceranic acid synthaseBy similarity (EC:2.3.1.287By similarity)Alternative name(s): Polyketide synthase pks2By similarity |
Gene namesi | Name:pks2 Ordered Locus Names:BCG_3888c |
Organismi | Mycobacterium bovis (strain BCG / Pasteur 1173P2) |
Taxonomic identifieri | 410289 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000329010 | 1 – 2126 | Phthioceranic/hydroxyphthioceranic acid synthaseAdd BLAST | 2126 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2075 | O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation | 1 |
Keywords - PTMi
Phosphopantetheine, PhosphoproteinFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2040 – 2126 | CarrierPROSITE-ProRule annotationAdd BLAST | 87 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 449 | Beta-ketoacyl synthase (KS)By similarityAdd BLAST | 449 | |
Regioni | 449 – 549 | Linker domain (LD)By similarityAdd BLAST | 101 | |
Regioni | 550 – 849 | Acyltransferase (AT)By similarityAdd BLAST | 300 | |
Regioni | 909 – 1191 | Dehydratase (DH)By similarityAdd BLAST | 283 | |
Regioni | 1227 – 1398 | Pseudo beta-ketoacyl reductase (PsiKR)By similarityAdd BLAST | 172 | |
Regioni | 1426 – 1750 | Enoylreductase (ER)By similarityAdd BLAST | 325 | |
Regioni | 1772 – 2019 | Beta-ketoacyl reductase (KR)By similarityAdd BLAST | 248 |
Phylogenomic databases
HOGENOMi | CLU_000022_31_5_11 |
OMAi | MVQRETD |
Family and domain databases
Gene3Di | 1.10.1200.10, 1 hit 3.10.129.110, 1 hit 3.40.366.10, 1 hit 3.40.47.10, 1 hit |
InterProi | View protein in InterPro IPR001227, Ac_transferase_dom_sf IPR036736, ACP-like_sf IPR014043, Acyl_transferase IPR016035, Acyl_Trfase/lysoPLipase IPR013149, ADH_C IPR013154, ADH_N IPR011032, GroES-like_sf IPR018201, Ketoacyl_synth_AS IPR014031, Ketoacyl_synth_C IPR014030, Ketoacyl_synth_N IPR016036, Malonyl_transacylase_ACP-bd IPR036291, NAD(P)-bd_dom_sf IPR032821, PKS_assoc IPR020841, PKS_Beta-ketoAc_synthase_dom IPR020807, PKS_dehydratase IPR042104, PKS_dehydratase_sf IPR020843, PKS_ER IPR013968, PKS_KR IPR020806, PKS_PP-bd IPR009081, PP-bd_ACP IPR006162, Ppantetheine_attach_site IPR016039, Thiolase-like |
Pfami | View protein in Pfam PF00698, Acyl_transf_1, 1 hit PF08240, ADH_N, 1 hit PF00107, ADH_zinc_N, 1 hit PF16197, KAsynt_C_assoc, 1 hit PF00109, ketoacyl-synt, 1 hit PF02801, Ketoacyl-synt_C, 1 hit PF08659, KR, 1 hit PF00550, PP-binding, 1 hit PF14765, PS-DH, 1 hit |
SMARTi | View protein in SMART SM00827, PKS_AT, 1 hit SM00826, PKS_DH, 1 hit SM00829, PKS_ER, 1 hit SM00825, PKS_KS, 1 hit SM00823, PKS_PP, 1 hit |
SUPFAMi | SSF47336, SSF47336, 1 hit SSF50129, SSF50129, 1 hit SSF51735, SSF51735, 3 hits SSF52151, SSF52151, 1 hit SSF53901, SSF53901, 1 hit SSF55048, SSF55048, 1 hit |
PROSITEi | View protein in PROSITE PS00606, B_KETOACYL_SYNTHASE, 1 hit PS50075, CARRIER, 1 hit PS00012, PHOSPHOPANTETHEINE, 1 hit |
i Sequence
Sequence statusi: Complete.
A1KQG0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGLGSAASGT GADRGAWTLA EPRVTPVAVI GMACRLPGGI DSPELLWKAL
60 70 80 90 100
LRGDDLITEV PPDRWDCDEF YDPQPGVPGR TVCKWGGFLD NPADFDCEFF
110 120 130 140 150
GIGEREAIAI DPQQRLLLET SWEAMEHAGL TQQTLAGSAT GVFAGVTHGD
160 170 180 190 200
YTMVAADAKQ LEEPYGYLGN SFSMASGRVA YAMRLHGPAI TVDTACSSGL
210 220 230 240 250
TAVHMACRSL HEGESDVALA GGVALMLEPR KAAAGSALGM LSPTGRCRAF
260 270 280 290 300
DVAADGFVSG EGCAVVVLKR LPDALADGDR ILAVIRGTSA NQDGHTVNIA
310 320 330 340 350
TPSQPAQVAA YRAALAAGGV DAATVGMVEA HGPGTPIGDP IEYASVSEVY
360 370 380 390 400
GVDGPCALAS VKTNFGHTQS TAGVLGLIKV VLALKHGVVP RNLHFTRLPD
410 420 430 440 450
EIAGITTNLF VPEVTTPWPT NGRQVPRRAA VSSYGFSGTN VHAVVEQAPQ
460 470 480 490 500
TEAQPHAAST PPTGTPALFT LSASSADALR QTAQRLTDWI QQHADSLVLS
510 520 530 540 550
DLAYTLARRR THRSVRTAVI ASSVDELIAG LGEVADGDTV YQPAVGQDDR
560 570 580 590 600
GPVWLFSGQG SQWAAMGADL LTNESVFAAT VAELEPLIAA ESGFSVTEAM
610 620 630 640 650
TAPETVTGID RVQPTIFAMQ VALAATMAAY GVRPGAVIGH SMGESAAAVV
660 670 680 690 700
AGVLSAEDGV RVICRRSKLM ATIAGSAAMA SVELPALAVQ SELTALGIDD
710 720 730 740 750
VVVAVVTAPQ STVIAGGTES VRKLVDIWER RDVLARAVAV DVASHSPQVD
760 770 780 790 800
PILDELIAAL ADLNPKAPEI PYYSATLFDP REAPACDARY WADNLRHTVR
810 820 830 840 850
FSAAVRSALD DGYRVFAELS PHPLLTHAGD QIAGSVGMPV AALAGMRREQ
860 870 880 890 900
PLPLGLRRLL TDLHNAGAAV DFSVLCPQGR LVDAPLPAWS HRFLFYDREG
910 920 930 940 950
VDNRSPGGST VAVHPLLGAH VRLPEEPERH AWQADVGTAT LPWLGDHRIH
960 970 980 990 1000
NVAALPGAAY CEMALSAARA VLGEQSEVRD MRFEAMLLLD DQTPVSTVAT
1010 1020 1030 1040 1050
VTSPGVVDFA VEALQEGVGH HLRRASAVLQ QVSGECEPPA YDMASLLEAH
1060 1070 1080 1090 1100
PCRVDGEDLR RQFDKHGVQY GPAFTGLAVA YVAEDATATM LAEVALPGSI
1110 1120 1130 1140 1150
RSQQGLYAIH PALLDACFQS VGAHPDSQSV GSGLLVPLGV RRVRAYAPVR
1160 1170 1180 1190 1200
TARYCYTRVT KVELVGVEAD IDVLDAHGTV LLAVCGLRIG TGVSERDKHN
1210 1220 1230 1240 1250
RVLNERLLTI EWHQRELPEM DPSGAGKWLL ISDCAASDVT ATRLADAFRE
1260 1270 1280 1290 1300
HSAACTTMRW PLHDDQLAAA DQLRDQVGSD EFSGVVVLTG SNTGTPHQGS
1310 1320 1330 1340 1350
ADRGAEYVRR LVGIARELSD LPGAVPRMYV VTRGAQRVLA DDCVNLEQGG
1360 1370 1380 1390 1400
LRGLLRTIGA EHPHLRATQI DVDEQTGVEQ LARQLLATSE EDETAWRDNE
1410 1420 1430 1440 1450
WYVARLCPTP LRPQERRTIV ADHQQSGMRL QIRTPGDMQT IELAAFHRVP
1460 1470 1480 1490 1500
PGPGQIEVAV RASSVNFADV LIAFGRYPSF EGHLPQLGTD FAGVVTAVGP
1510 1520 1530 1540 1550
GVTDHKVGDH VGGMSPNGCW GTFVTCDARL AATLPPGLGD AQAAAVTTAH
1560 1570 1580 1590 1600
ATAWYGLHEL ARIRAGDTVL IHSGTGGVGQ AAIAIARAAG AEIFATAGTP
1610 1620 1630 1640 1650
QRRELLRNMG IEHVYDSRSI EFAEQIRRDT NGRGVDVVLN SVTGAAQLAG
1660 1670 1680 1690 1700
LKLLAFRGRF VEIGKRDIYG DTKLGLFPFR RNLSFYAVDL GLLSATHPEE
1710 1720 1730 1740 1750
LRDLLGTVYR LTAAGELPMP QSTHYPLVEA ATAIRVMGNA EHTGKLVLHI
1760 1770 1780 1790 1800
PQTGKSLVTL PPEQAQVFRP DGSYIITGGL GGLGLFLAEK MAAAGCGRIV
1810 1820 1830 1840 1850
LNSRTQPTQK MRETIEAIAA MGSEVVVECG DIAQPGTAER LVATAVATGL
1860 1870 1880 1890 1900
PVRGVLHAAA VVEDATLANI TDELLARDWA PKVHGAWELH EATSGQPLDW
1910 1920 1930 1940 1950
FCLFSSAAAL TGSPGQSAYS AANSWLDAFA HWRQAQGLPA TAIAWGAWSD
1960 1970 1980 1990 2000
IGQLGWWSAS PARASALEES NYTAITPDEG AYAFEALLRH NRVYTGYAPV
2010 2020 2030 2040 2050
IGAPWLVAFA ERSRFFEVFS SSNGSGTSKF RVELNELPRD EWPARLRQLV
2060 2070 2080 2090 2100
AEQVSLILRR TVDPDRPLPE YGLDSLGALE LRTRIETETG IRLAPKNVSA
2110 2120
TVRGLADHLY EQLAPDDAPA AALSSQ
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AM408590 Genomic DNA Translation: CAL73878.1 |
RefSeqi | WP_011799379.1, NC_008769.1 |
Genome annotation databases
KEGGi | mbb:BCG_3888c |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AM408590 Genomic DNA Translation: CAL73878.1 |
RefSeqi | WP_011799379.1, NC_008769.1 |
3D structure databases
SMRi | A1KQG0 |
ModBasei | Search... |
Genome annotation databases
KEGGi | mbb:BCG_3888c |
Phylogenomic databases
HOGENOMi | CLU_000022_31_5_11 |
OMAi | MVQRETD |
Family and domain databases
Gene3Di | 1.10.1200.10, 1 hit 3.10.129.110, 1 hit 3.40.366.10, 1 hit 3.40.47.10, 1 hit |
InterProi | View protein in InterPro IPR001227, Ac_transferase_dom_sf IPR036736, ACP-like_sf IPR014043, Acyl_transferase IPR016035, Acyl_Trfase/lysoPLipase IPR013149, ADH_C IPR013154, ADH_N IPR011032, GroES-like_sf IPR018201, Ketoacyl_synth_AS IPR014031, Ketoacyl_synth_C IPR014030, Ketoacyl_synth_N IPR016036, Malonyl_transacylase_ACP-bd IPR036291, NAD(P)-bd_dom_sf IPR032821, PKS_assoc IPR020841, PKS_Beta-ketoAc_synthase_dom IPR020807, PKS_dehydratase IPR042104, PKS_dehydratase_sf IPR020843, PKS_ER IPR013968, PKS_KR IPR020806, PKS_PP-bd IPR009081, PP-bd_ACP IPR006162, Ppantetheine_attach_site IPR016039, Thiolase-like |
Pfami | View protein in Pfam PF00698, Acyl_transf_1, 1 hit PF08240, ADH_N, 1 hit PF00107, ADH_zinc_N, 1 hit PF16197, KAsynt_C_assoc, 1 hit PF00109, ketoacyl-synt, 1 hit PF02801, Ketoacyl-synt_C, 1 hit PF08659, KR, 1 hit PF00550, PP-binding, 1 hit PF14765, PS-DH, 1 hit |
SMARTi | View protein in SMART SM00827, PKS_AT, 1 hit SM00826, PKS_DH, 1 hit SM00829, PKS_ER, 1 hit SM00825, PKS_KS, 1 hit SM00823, PKS_PP, 1 hit |
SUPFAMi | SSF47336, SSF47336, 1 hit SSF50129, SSF50129, 1 hit SSF51735, SSF51735, 3 hits SSF52151, SSF52151, 1 hit SSF53901, SSF53901, 1 hit SSF55048, SSF55048, 1 hit |
PROSITEi | View protein in PROSITE PS00606, B_KETOACYL_SYNTHASE, 1 hit PS50075, CARRIER, 1 hit PS00012, PHOSPHOPANTETHEINE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PHAS_MYCBP | |
Accessioni | A1KQG0Primary (citable) accession number: A1KQG0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 8, 2008 |
Last sequence update: | February 6, 2007 | |
Last modified: | February 23, 2022 | |
This is version 89 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |