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Protein

Phosphoribosylformylglycinamidine synthase subunit PurL

Gene

purL

Organism
Mycobacterium bovis (strain BCG / Pasteur 1173P2)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.UniRule annotation

Catalytic activityi

ATP + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine + L-glutamate.UniRule annotation

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Phosphoribosylformylglycinamidine synthase subunit PurQ (purQ), Phosphoribosylformylglycinamidine synthase subunit PurS (purS), Phosphoribosylformylglycinamidine synthase subunit PurL (purL)
  2. Phosphoribosylformylglycinamidine cyclo-ligase (purM)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei66UniRule annotation1
Binding sitei69ATPUniRule annotation1
Binding sitei113ATPUniRule annotation1
Metal bindingi115Magnesium 1UniRule annotation1
Active sitei117Proton acceptorUniRule annotation1
Binding sitei138SubstrateUniRule annotation1
Metal bindingi139Magnesium 2UniRule annotation1
Binding sitei264SubstrateUniRule annotation1
Metal bindingi292Magnesium 2UniRule annotation1
Binding sitei524ATPUniRule annotation1
Binding sitei561ATP; via amide nitrogen and carbonyl oxygenUniRule annotation1
Metal bindingi562Magnesium 1UniRule annotation1
Binding sitei564SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processPurine biosynthesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayi
UPA00074;UER00128

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylformylglycinamidine synthase subunit PurLUniRule annotation (EC:6.3.5.3UniRule annotation)
Short name:
FGAM synthaseUniRule annotation
Alternative name(s):
Formylglycinamide ribonucleotide amidotransferase subunit IIUniRule annotation
Short name:
FGAR amidotransferase IIUniRule annotation
Short name:
FGAR-AT IIUniRule annotation
Glutamine amidotransferase PurLUniRule annotation
Phosphoribosylformylglycinamidine synthase subunit IIUniRule annotation
Gene namesi
Name:purLUniRule annotation
Ordered Locus Names:BCG_0855
OrganismiMycobacterium bovis (strain BCG / Pasteur 1173P2)
Taxonomic identifieri410289 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001472 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000503241 – 766Phosphoribosylformylglycinamidine synthase subunit PurLAdd BLAST766

Interactioni

Subunit structurei

Monomer. Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA1KGT6
SMRiA1KGT6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni116 – 119Substrate bindingUniRule annotation4
Regioni336 – 338Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the FGAMS family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000238227
KOiK01952

Family and domain databases

Gene3Di3.30.1330.10, 2 hits
3.90.650.10, 4 hits
HAMAPiMF_00420 PurL_2, 1 hit
InterProiView protein in InterPro
IPR010074 PRibForGlyAmidine_synth_PurL
IPR010918 PurM-like_C_dom
IPR036676 PurM-like_C_sf
IPR016188 PurM-like_N
IPR036921 PurM-like_N_sf
PANTHERiPTHR43555 PTHR43555, 1 hit
PfamiView protein in Pfam
PF00586 AIRS, 2 hits
PF02769 AIRS_C, 2 hits
PIRSFiPIRSF001587 FGAM_synthase_II, 1 hit
SUPFAMiSSF55326 SSF55326, 2 hits
SSF56042 SSF56042, 2 hits
TIGRFAMsiTIGR01736 FGAM_synth_II, 1 hit

Sequencei

Sequence statusi: Complete.

A1KGT6-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSPLARTPRK TSVLDTVEHA ATTPDQPQPY GELGLKDDEY RRIRQILGRR
60 70 80 90 100
PTDTELAMYS VMWSEHCSYK SSKVHLRYFG ETTSDEMRAA MLAGIGENAG
110 120 130 140 150
VVDIGDGWAV TFKVESHNHP SYVEPYQGAA TGVGGIVRDI MAMGARPVAV
160 170 180 190 200
MDQLRFGAAD APDTRRVLDG VVRGIGGYGN SLGLPNIGGE TVFDPCYAGN
210 220 230 240 250
PLVNALCVGV LRQEDLHLAF ASGAGNKIIL FGARTGLDGI GGVSVLASDT
260 270 280 290 300
FDAEGSRKKL PSVQVGDPFM EKVLIECCLE LYAGGLVIGI QDLGGAGLSC
310 320 330 340 350
ATSELASAGD GGMTIQLDSV PLRAKEMTPA EVLCSESQER MCAVVSPKNV
360 370 380 390 400
DAFLAVCRKW EVLATVIGEV TDGDRLQITW HGETVVDVPP RTVAHEGPVY
410 420 430 440 450
QRPVARPDTQ DALNADRSAK LSRPVTGDEL RATLLALLGS PHLCSRAFIT
460 470 480 490 500
EQYDRYVRGN TVLAEHADGG MLRIDESTGR GIAVSTDASG RYTLLDPYAG
510 520 530 540 550
AQLALAEAYR NVAVTGATPV AVTNCLNFGS PEDPGVMWQF TQAVRGLADG
560 570 580 590 600
CADLGIPVTG GNVSFYNQTG SAAILPTPVV GVLGVIDDVR RRIPTGLGAE
610 620 630 640 650
PGETLMLLGD TRDEFDGSVW AQVTADHLGG LPPVVDLARE KLLAAVLSSA
660 670 680 690 700
SRDGLVSAAH DLSEGGLAQA IVESALAGET GCRIVLPEGA DPFVLLFSES
710 720 730 740 750
AGRVLVAVPR TEESRFRGMC EARGLPAVRI GVVDQGSDAV EVQGLFAVSL
760
AELRATSEAV LPRYFG
Length:766
Mass (Da):80,802
Last modified:January 7, 2015 - v2
Checksum:iEFE6F9C0F7C2CE52
GO

Sequence cautioni

The sequence CAL70841 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM408590 Genomic DNA Translation: CAL70841.1 Different initiation.

Genome annotation databases

EnsemblBacteriaiCAL70841; CAL70841; BCG_0855
KEGGimbb:BCG_0855

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM408590 Genomic DNA Translation: CAL70841.1 Different initiation.

3D structure databases

ProteinModelPortaliA1KGT6
SMRiA1KGT6
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAL70841; CAL70841; BCG_0855
KEGGimbb:BCG_0855

Phylogenomic databases

HOGENOMiHOG000238227
KOiK01952

Enzyme and pathway databases

UniPathwayi
UPA00074;UER00128

Family and domain databases

Gene3Di3.30.1330.10, 2 hits
3.90.650.10, 4 hits
HAMAPiMF_00420 PurL_2, 1 hit
InterProiView protein in InterPro
IPR010074 PRibForGlyAmidine_synth_PurL
IPR010918 PurM-like_C_dom
IPR036676 PurM-like_C_sf
IPR016188 PurM-like_N
IPR036921 PurM-like_N_sf
PANTHERiPTHR43555 PTHR43555, 1 hit
PfamiView protein in Pfam
PF00586 AIRS, 2 hits
PF02769 AIRS_C, 2 hits
PIRSFiPIRSF001587 FGAM_synthase_II, 1 hit
SUPFAMiSSF55326 SSF55326, 2 hits
SSF56042 SSF56042, 2 hits
TIGRFAMsiTIGR01736 FGAM_synth_II, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPURL_MYCBP
AccessioniPrimary (citable) accession number: A1KGT6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 7, 2015
Last modified: February 28, 2018
This is version 77 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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