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Protein

Methionine--tRNA ligase

Gene

metG

Organism
Azoarcus sp. (strain BH72)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.UniRule annotation

Catalytic activityi

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi143ZincUniRule annotation1
Metal bindingi146ZincUniRule annotation1
Metal bindingi156ZincUniRule annotation1
Metal bindingi159ZincUniRule annotation1
Binding sitei348ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase, RNA-binding, tRNA-binding
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciASP62928:AZO_RS16095-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine--tRNA ligaseUniRule annotation (EC:6.1.1.10UniRule annotation)
Alternative name(s):
Methionyl-tRNA synthetaseUniRule annotation
Short name:
MetRSUniRule annotation
Gene namesi
Name:metGUniRule annotation
Ordered Locus Names:azo3203
OrganismiAzoarcus sp. (strain BH72)
Taxonomic identifieri62928 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaRhodocyclalesZoogloeaceaeAzoarcus
Proteomesi
  • UP000002588 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003317791 – 723Methionine--tRNA ligaseAdd BLAST723

Proteomic databases

PRIDEiA1KAG3

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi62928.azo3203

Structurei

3D structure databases

ProteinModelPortaliA1KAG3
SMRiA1KAG3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini612 – 723tRNA-bindingUniRule annotationAdd BLAST112

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi12 – 22"HIGH" regionAdd BLAST11
Motifi345 – 349"KMSKS" region5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CKH Bacteria
COG0073 LUCA
COG0143 LUCA
HOGENOMiHOG000200400
KOiK01874
OMAiTYVWFDA
OrthoDBiPOG091H0269

Family and domain databases

CDDicd00814 MetRS_core, 1 hit
cd02800 tRNA_bind_EcMetRS_like, 1 hit
Gene3Di2.20.28.20, 1 hit
3.40.50.620, 2 hits
HAMAPiMF_00098 Met_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR004495 Met-tRNA-synth_bsu_C
IPR023458 Met-tRNA_ligase_1
IPR014758 Met-tRNA_synth
IPR015413 Methionyl/Leucyl_tRNA_Synth
IPR033911 MetRS_core
IPR029038 MetRS_Zn
IPR012340 NA-bd_OB-fold
IPR014729 Rossmann-like_a/b/a_fold
IPR002547 tRNA-bd_dom
IPR009080 tRNAsynth_Ia_anticodon-bd
PfamiView protein in Pfam
PF09334 tRNA-synt_1g, 1 hit
PF01588 tRNA_bind, 1 hit
PRINTSiPR01041 TRNASYNTHMET
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50249 SSF50249, 1 hit
SSF57770 SSF57770, 1 hit
TIGRFAMsiTIGR00398 metG, 1 hit
TIGR00399 metG_C_term, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit
PS50886 TRBD, 1 hit

Sequencei

Sequence statusi: Complete.

A1KAG3-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSRKILVTNA LPYANGDIHL GHLVGYIQGD IWVRFQRMRG NSVHYVCADD
60 70 80 90 100
THGTPIMLRA EKEGITPEQL IDRVHGEHLR DFTDFGVGFD HYHSTHSAEN
110 120 130 140 150
RAYAEEIYGK LLGAGFIETR SIEQFYDPVK EMFLPDRFIK GECPKCGAGD
160 170 180 190 200
QYGDNCEVCG AAYAPTELKN PYSAVSGAKP VLRTSEHYFF RLSDPRAVEF
210 220 230 240 250
LRDWTRGTNA GGERRLQAEA ANKMKEWLGE AGENKLSDWD ISRDAPYFGF
260 270 280 290 300
EIPGAPGKYF YVWLDAPIGY LASFRHYAER RGDIAVADYT DAARAESHGT
310 320 330 340 350
ELVHFIGKDI LYFHALFWPA MLKFAGYRTP TQLCVNGFLT VDGAKMSKSR
360 370 380 390 400
GTFITARSYI VQGLNPEWLR YYFASKSNGT MEDVDLNLDD MIAKVNSDLV
410 420 430 440 450
GKYVNIASRC AGFIAKRFDG KLGASDPKAT ADFESAYESA QIAQAYDERD
460 470 480 490 500
YGRALREIMR LADLANQYVN DHKPWELAKQ DGQDAQLHVV CSTALTLFRD
510 520 530 540 550
LTLYLKPVLP GLAAKVEAFL ALPPLQWNDS WKPLPAGHAI TAYQHLMTRV
560 570 580 590 600
ERKQIDALLE ANRESLAPAA ATAPAKDAKP AKEAGSQQRH AEKQQHAAGV
610 620 630 640 650
SETASPHISI DDFTKVDLRI AKIVDAQHVE GADKLIRLQL DIGETDEAGN
660 670 680 690 700
PKPRQVFAGI KSAYDPATLV GRLTVMVANL APRKMKFGMS EGMVLAASDP
710 720
DGKTGGLYIL APDEGAQAGM RVK
Length:723
Mass (Da):80,120
Last modified:February 6, 2007 - v1
Checksum:i1FDA65CAC43E41E7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM406670 Genomic DNA Translation: CAL95819.1
RefSeqiWP_011766927.1, NC_008702.1

Genome annotation databases

EnsemblBacteriaiCAL95819; CAL95819; azo3203
KEGGiazo:azo3203

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM406670 Genomic DNA Translation: CAL95819.1
RefSeqiWP_011766927.1, NC_008702.1

3D structure databases

ProteinModelPortaliA1KAG3
SMRiA1KAG3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi62928.azo3203

Proteomic databases

PRIDEiA1KAG3

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAL95819; CAL95819; azo3203
KEGGiazo:azo3203

Phylogenomic databases

eggNOGiENOG4105CKH Bacteria
COG0073 LUCA
COG0143 LUCA
HOGENOMiHOG000200400
KOiK01874
OMAiTYVWFDA
OrthoDBiPOG091H0269

Enzyme and pathway databases

BioCyciASP62928:AZO_RS16095-MONOMER

Family and domain databases

CDDicd00814 MetRS_core, 1 hit
cd02800 tRNA_bind_EcMetRS_like, 1 hit
Gene3Di2.20.28.20, 1 hit
3.40.50.620, 2 hits
HAMAPiMF_00098 Met_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR004495 Met-tRNA-synth_bsu_C
IPR023458 Met-tRNA_ligase_1
IPR014758 Met-tRNA_synth
IPR015413 Methionyl/Leucyl_tRNA_Synth
IPR033911 MetRS_core
IPR029038 MetRS_Zn
IPR012340 NA-bd_OB-fold
IPR014729 Rossmann-like_a/b/a_fold
IPR002547 tRNA-bd_dom
IPR009080 tRNAsynth_Ia_anticodon-bd
PfamiView protein in Pfam
PF09334 tRNA-synt_1g, 1 hit
PF01588 tRNA_bind, 1 hit
PRINTSiPR01041 TRNASYNTHMET
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50249 SSF50249, 1 hit
SSF57770 SSF57770, 1 hit
TIGRFAMsiTIGR00398 metG, 1 hit
TIGR00399 metG_C_term, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit
PS50886 TRBD, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSYM_AZOSB
AccessioniPrimary (citable) accession number: A1KAG3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: February 6, 2007
Last modified: October 10, 2018
This is version 83 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
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Main funding by: National Institutes of Health

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