UniProtKB - A1IGV8 (AAGAR_THASX)
Protein
Alpha-agarase
Gene
agaA33
Organism
Thalassotalea agarivorans (Thalassomonas agarivorans)
Status
Functioni
Alpha-agarase. Hydrolyzes agarose, agarohexaose, neoagarohexaose and porphyran. Hydrolysis of porphyran by this enzyme improves its antioxidant activity. Does not hydrolyze kappa-carrageenan, iota-carrageenen or lambda-carrageenan.2 Publications
Catalytic activityi
- Endohydrolysis of 1,3-alpha-L-galactosidic linkages in agarose, yielding agarotetraose as the major product.2 Publications EC:3.2.1.158
Cofactori
Ca2+1 Publication
pH dependencei
Optimum pH is 8.5. Active between pH 4.5 and 9.5, stable between pH 6.5 and 10.5.2 Publications
Temperature dependencei
Optimum temperature is 45 degrees Celsius. Stable up to 40 degrees Celsius.2 Publications
GO - Molecular functioni
- alpha-agarase activity Source: UniProtKB-EC
- calcium ion binding Source: InterPro
- carbohydrate binding Source: InterPro
GO - Biological processi
- cell adhesion Source: InterPro
- polysaccharide catabolic process Source: UniProtKB-KW
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Biological process | Carbohydrate metabolism, Polysaccharide degradation |
Ligand | Calcium, Metal-binding |
Enzyme and pathway databases
BioCyci | MetaCyc:MONOMER-16654 |
Protein family/group databases
CAZyi | CBM6, Carbohydrate-Binding Module Family 6 GH96, Glycoside Hydrolase Family 96 |
Names & Taxonomyi
Protein namesi | Recommended name: Alpha-agarase1 Publication (EC:3.2.1.158)Alternative name(s): AgaraseA331 Publication |
Gene namesi | Name:agaA33Imported |
Organismi | Thalassotalea agarivorans (Thalassomonas agarivorans) |
Taxonomic identifieri | 349064 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Colwelliaceae › Thalassotalea |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 27 | Sequence analysisAdd BLAST | 27 | |
PropeptideiPRO_0000397928 | 28 – 684 | Sequence analysis1 PublicationAdd BLAST | 657 | |
ChainiPRO_0000397929 | 685 – 1463 | Alpha-agarase1 PublicationAdd BLAST | 779 |
Interactioni
Subunit structurei
Monomer.
1 PublicationFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 534 – 677 | PA14PROSITE-ProRule annotationAdd BLAST | 144 | |
Domaini | 701 – 832 | CBM6PROSITE-ProRule annotationAdd BLAST | 132 |
Sequence similaritiesi
Belongs to the glycosyl hydrolase 96 family.By similarity
Keywords - Domaini
SignalFamily and domain databases
Gene3Di | 2.60.120.260, 3 hits 4.10.1080.10, 1 hit |
InterProi | View protein in InterPro IPR006584, Cellulose-bd_IV IPR005084, CMB_fam6 IPR008979, Galactose-bd-like_sf IPR037524, PA14/GLEYA IPR003367, Thrombospondin_3-like_rpt IPR028974, TSP_type-3_rpt |
Pfami | View protein in Pfam PF03422, CBM_6, 1 hit PF02412, TSP_3, 6 hits |
SMARTi | View protein in SMART SM00606, CBD_IV, 1 hit |
SUPFAMi | SSF103647, SSF103647, 2 hits SSF49785, SSF49785, 3 hits |
PROSITEi | View protein in PROSITE PS51175, CBM6, 3 hits PS51820, PA14, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
A1IGV8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MITSSKKIVS AMLSTSLWIG VASAAYAETT NVEAEGYSTI GGTYQDGNPQ
60 70 80 90 100
PINIYSVNGV QAINFVNRGD FAEYDVSVST AGEYSIEYLI GTSIASGSAV
110 120 130 140 150
EISVLVDGNW QSAGSTNVPL GQWDNFQALA ANNNISLAQG TNRIKITGAG
160 170 180 190 200
THDWQWNLDA FSLTLVTPEN PDNPDNPDNP DDGNTGQPGT PFTIEMEAFD
210 220 230 240 250
ATGSDDPRAQ GMVIGERGYP EDKHTVVDSN QTTDWVDYNI NFPVSGNYRI
260 270 280 290 300
EMLASGQTSH ATAILFVDNV QINEVAVDTG NQAVFLDFEL TDSTYISAGA
310 320 330 340 350
HTIRVQSGSQ INEFSWMWFG DALTFTPLDG GSTDGDADND GVLDSVDTCP
360 370 380 390 400
NTPAGAQVDA NGCEIIVDND TDNDGVDNSI DQCPNTPAGA QVDANGCEIV
410 420 430 440 450
AVVDADNDGV EDSLDMCPNT PAGAPVNGQG CADSQLDADN DGVSDDIDQC
460 470 480 490 500
PSTPAGSVVD GTGCIVVTPP ADSDNDGVVD TLDMCPNTAA GLTVDSQGCA
510 520 530 540 550
LSQLDSDNDG VTDDIDQCAN TPSGETANAT GCSSSQEGGG TDPDTPQPGL
560 570 580 590 600
LYGELAGAMN VSDTNPNWER TTDLLQTEDS VKGNTTEVYT GFIYDADGHI
610 620 630 640 650
SFYEHIDDSV RLYIDGVLVL SNDSWEASSQ TTDLNLTPGT HEIELRIGNA
660 670 680 690 700
DGGSGAVDGI GFGIDVDGGT NFVHPSTLSE SIFTSVGEET GNPDLEQEGD
710 720 730 740 750
IIVELESFVF TSTNGRVGSD SVEGFSPTAT GVNWVTNGDY GDYMVTFEEP
760 770 780 790 800
GTYGAYITIS AANDGSYGAR VDVDGWPVAW GYFGGTGSWD VSSENLLYGG
810 820 830 840 850
TFVVEQAGEK VVRVEAIGGS DWQWSGDRVR FTRLGDVTAI PSPIYNPDDH
860 870 880 890 900
FVAEIQGPQT DVTYLKKPVE IPANKKVLKS DVWYTYPQNR ELEGYDNFGA
910 920 930 940 950
TGAFWGHPPE HDFYDDTVIM DWAVDAVYAF QAEGYEYTAR GEFDWGYGWF
960 970 980 990 1000
TEYTTNPQPH YVRTLDDRNV RMTFMGYLSH DGYNNNWLSN HSPAFVPFMK
1010 1020 1030 1040 1050
SQVDQILKAN PDKLMFDTQT NSTRSTDMRD FGGDFSPYAM ENFRVWLSKK
1060 1070 1080 1090 1100
YSTGELAALG INDINSFDYG DFLRAQGVTH TSWSNAGDTL SGNIPLQEDY
1110 1120 1130 1140 1150
IYFNRDVWNQ KFAEVLDYIR QQQPDIEIGA STHLFESRGY VFNENLTFLS
1160 1170 1180 1190 1200
GELNLGARTT ISELPTNILV HLKGAQAVDK TLVYFPYPWE FDELRLQDAP
1210 1220 1230 1240 1250
RFGRGWVAQA YAYGGLFSIP ANVWVGGEVW TWSPGADNYR DIYLFVRAQA
1260 1270 1280 1290 1300
DLLDDYTSYS KVGLVHAMYS SMKAGFIDGG NQIQSSTKLL TEGNINFDLL
1310 1320 1330 1340 1350
VFGDEGYPVV PRPEDFDKFD HIFFDGDEQY LTAEQQALLD QQGDKVRHIG
1360 1370 1380 1390 1400
QRGTVSGIEI TVSISGTESN ETVSAVSRIH ETDAAAPYVV HLVNRPFAGG
1410 1420 1430 1440 1450
VTPTLNNVEV AIPQSYFPEV VTGATLHLPD GTSTSLTLST NADGDVVLPV
1460
NNLEVWGILE LAH
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB211981 Genomic DNA Translation: BAF44076.1 |
Genome annotation databases
KEGGi | ag:BAF44076 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB211981 Genomic DNA Translation: BAF44076.1 |
3D structure databases
SMRi | A1IGV8 |
ModBasei | Search... |
Protein family/group databases
CAZyi | CBM6, Carbohydrate-Binding Module Family 6 GH96, Glycoside Hydrolase Family 96 |
Genome annotation databases
KEGGi | ag:BAF44076 |
Enzyme and pathway databases
BioCyci | MetaCyc:MONOMER-16654 |
Family and domain databases
Gene3Di | 2.60.120.260, 3 hits 4.10.1080.10, 1 hit |
InterProi | View protein in InterPro IPR006584, Cellulose-bd_IV IPR005084, CMB_fam6 IPR008979, Galactose-bd-like_sf IPR037524, PA14/GLEYA IPR003367, Thrombospondin_3-like_rpt IPR028974, TSP_type-3_rpt |
Pfami | View protein in Pfam PF03422, CBM_6, 1 hit PF02412, TSP_3, 6 hits |
SMARTi | View protein in SMART SM00606, CBD_IV, 1 hit |
SUPFAMi | SSF103647, SSF103647, 2 hits SSF49785, SSF49785, 3 hits |
PROSITEi | View protein in PROSITE PS51175, CBM6, 3 hits PS51820, PA14, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AAGAR_THASX | |
Accessioni | A1IGV8Primary (citable) accession number: A1IGV8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 5, 2010 |
Last sequence update: | February 6, 2007 | |
Last modified: | December 2, 2020 | |
This is version 49 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencingDocuments
- Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries - SIMILARITY comments
Index of protein domains and families