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UniProtKB - A1DG68 (LKHA4_NEOFI)
Protein
Leucine aminopeptidase 2
Gene
NFIA_083260
Organism
Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) (Aspergillus fischerianus)
Status
Functioni
Aminopeptidase that preferentially cleaves di- and tripeptides. Also has low epoxide hydrolase activity (in vitro). Can hydrolyze the epoxide leukotriene LTA4 but it forms preferentially 5,6-dihydroxy-7,9,11,14-eicosatetraenoic acid rather than the cytokine leukotriene B4 as the product compared to the homologous mammalian enzyme (in vitro).
By similarityCatalytic activityi
- EC:3.3.2.10By similarity
Cofactori
Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 300 | Zinc; catalyticPROSITE-ProRule annotation | 1 | |
Active sitei | 301 | Proton acceptorPROSITE-ProRule annotation | 1 | |
Metal bindingi | 304 | Zinc; catalyticPROSITE-ProRule annotation | 1 | |
Metal bindingi | 323 | Zinc; catalyticPROSITE-ProRule annotation | 1 | |
Active sitei | 388 | Proton donorPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- aminopeptidase activity Source: UniProtKB
- epoxide hydrolase activity Source: UniProtKB
- metallopeptidase activity Source: UniProtKB-KW
- zinc ion binding Source: UniProtKB
GO - Biological processi
- peptide catabolic process Source: UniProtKB
Keywordsi
Molecular function | Hydrolase, Metalloprotease, Protease |
Ligand | Metal-binding, Zinc |
Protein family/group databases
MEROPSi | M01.034 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | ORF Names:NFIA_083260 |
Organismi | Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) (Aspergillus fischerianus) |
Taxonomic identifieri | 331117 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Aspergillaceae › Aspergillus › Aspergillus subgen. Fumigati › |
Proteomesi |
|
Organism-specific databases
VEuPathDBi | FungiDB:NFIA_083260 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000324933 | 1 – 617 | Leucine aminopeptidase 2Add BLAST | 617 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 139 – 141 | Substrate bindingBy similarity | 3 | |
Regioni | 271 – 276 | Substrate bindingBy similarity | 6 |
Sequence similaritiesi
Belongs to the peptidase M1 family.Curated
Phylogenomic databases
eggNOGi | KOG1047, Eukaryota |
HOGENOMi | CLU_014505_1_1_1 |
OMAi | YHPICRQ |
OrthoDBi | 775595at2759 |
Family and domain databases
CDDi | cd09599, M1_LTA4H, 1 hit |
Gene3Di | 1.10.390.10, 1 hit 1.25.40.320, 1 hit 2.60.40.1730, 1 hit |
InterProi | View protein in InterPro IPR045357, Aminopeptidase_N-like_N IPR042097, Aminopeptidase_N-like_N_sf IPR016024, ARM-type_fold IPR012777, LTA4H IPR038502, M1_LTA-4_hydro/amino_C_sf IPR034015, M1_LTA4H IPR001930, Peptidase_M1 IPR015211, Peptidase_M1_C IPR014782, Peptidase_M1_dom IPR027268, Peptidase_M4/M1_CTD_sf |
PANTHERi | PTHR45726, PTHR45726, 1 hit |
Pfami | View protein in Pfam PF09127, Leuk-A4-hydro_C, 1 hit PF01433, Peptidase_M1, 1 hit PF17900, Peptidase_M1_N, 1 hit |
PRINTSi | PR00756, ALADIPTASE |
SMARTi | View protein in SMART SM01263, Leuk-A4-hydro_C, 1 hit |
SUPFAMi | SSF48371, SSF48371, 1 hit SSF63737, SSF63737, 1 hit |
TIGRFAMsi | TIGR02411, leuko_A4_hydro, 1 hit |
PROSITEi | View protein in PROSITE PS00142, ZINC_PROTEASE, 1 hit |
i Sequence
Sequence statusi: Complete.
A1DG68-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPTVVNPPRD PNTLSNYNNW VSTHITANFD ILFDQRKLAG SVIHQFRSTT
60 70 80 90 100
HGESNHIILD TNHLDIGSVK VNGQPSEWEY LPRLEPYGTP LKIKLDQGVK
110 120 130 140 150
LNEMIEVDIS VKTTEKCTAL QWLTPAQTSN KKHPYMFSQC QAIHARSIFP
160 170 180 190 200
CQDTPDVKCT LDFNITSPLP VIASGLPVRG SSEAPKSDGK TLYKFHQKVP
210 220 230 240 250
IPSYLFALAS GDISEAPIGP RSVVATSPDK LGECQWELEA DTEKFINAIE
260 270 280 290 300
KIVYPYVWGE YNVLILPPSF PYGGMENPIF TFATPSIISK DRENIDVIAH
310 320 330 340 350
ELAHSWSGNL VTNASWEHFW LNEGWTTYLE RRIVAAVHGE PYRHFSAIIG
360 370 380 390 400
WKALTDSVEH FGPEHDFTKL ITNLKGMDPD DAFSSIPYEK GFNFLFHLEN
410 420 430 440 450
LVGKSKFDLF IPHYFNKYKG KSLDSYEFKS TILDFFKDDS EASTALNELD
460 470 480 490 500
WDSWFYAPGL PPKPDFDTSL VDVVYDLAKK WLSLPNSSFK PQPEDIRGLT
510 520 530 540 550
ANQVVVFLEQ ILVSERQLTP ELSKLMGEIY GLAASQNIEV ANLYFQVGLQ
560 570 580 590 600
AGDASVLEPT ADLLGKIGRM KFVRPLYRKL AKFDRKRAVE TFEKHRDFYH
610
PICRAMVEKD LFGKKDE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DS027696 Genomic DNA Translation: EAW18375.1 |
RefSeqi | XP_001260272.1, XM_001260271.1 |
Genome annotation databases
EnsemblFungii | EAW18375; EAW18375; NFIA_083260 |
GeneIDi | 4586829 |
KEGGi | nfi:NFIA_083260 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DS027696 Genomic DNA Translation: EAW18375.1 |
RefSeqi | XP_001260272.1, XM_001260271.1 |
3D structure databases
AlphaFoldDBi | A1DG68 |
SMRi | A1DG68 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 36630.CADNFIAP00007509 |
Protein family/group databases
MEROPSi | M01.034 |
Genome annotation databases
EnsemblFungii | EAW18375; EAW18375; NFIA_083260 |
GeneIDi | 4586829 |
KEGGi | nfi:NFIA_083260 |
Organism-specific databases
VEuPathDBi | FungiDB:NFIA_083260 |
Phylogenomic databases
eggNOGi | KOG1047, Eukaryota |
HOGENOMi | CLU_014505_1_1_1 |
OMAi | YHPICRQ |
OrthoDBi | 775595at2759 |
Family and domain databases
CDDi | cd09599, M1_LTA4H, 1 hit |
Gene3Di | 1.10.390.10, 1 hit 1.25.40.320, 1 hit 2.60.40.1730, 1 hit |
InterProi | View protein in InterPro IPR045357, Aminopeptidase_N-like_N IPR042097, Aminopeptidase_N-like_N_sf IPR016024, ARM-type_fold IPR012777, LTA4H IPR038502, M1_LTA-4_hydro/amino_C_sf IPR034015, M1_LTA4H IPR001930, Peptidase_M1 IPR015211, Peptidase_M1_C IPR014782, Peptidase_M1_dom IPR027268, Peptidase_M4/M1_CTD_sf |
PANTHERi | PTHR45726, PTHR45726, 1 hit |
Pfami | View protein in Pfam PF09127, Leuk-A4-hydro_C, 1 hit PF01433, Peptidase_M1, 1 hit PF17900, Peptidase_M1_N, 1 hit |
PRINTSi | PR00756, ALADIPTASE |
SMARTi | View protein in SMART SM01263, Leuk-A4-hydro_C, 1 hit |
SUPFAMi | SSF48371, SSF48371, 1 hit SSF63737, SSF63737, 1 hit |
TIGRFAMsi | TIGR02411, leuko_A4_hydro, 1 hit |
PROSITEi | View protein in PROSITE PS00142, ZINC_PROTEASE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | LKHA4_NEOFI | |
Accessioni | A1DG68Primary (citable) accession number: A1DG68 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 18, 2008 |
Last sequence update: | January 23, 2007 | |
Last modified: | May 25, 2022 | |
This is version 97 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - SIMILARITY comments
Index of protein domains and families