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UniProtKB - A1CP08 (MNS1B_ASPCL)
Protein
Probable mannosyl-oligosaccharide alpha-1,2-mannosidase 1B
Gene
mns1B
Organism
Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107)
Status
Functioni
Involved in the maturation of Asn-linked oligosaccharides. Progressively trims alpha-1,2-linked mannose residues from Man9GlcNAc2 to produce Man5GlcNAc2 (By similarity).
By similarityCatalytic activityi
- 4 H2O + N4-(α-D-Man-(1→2)-α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-α-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 9A1,2,3B1,2,3) = 4 β-D-mannose + N4-(α-D-Man-(1→3)-[α-D-Man-(1→3)-[α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-β-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2)By similarityEC:3.2.1.113By similarity
- 3 H2O + N4-(α-D-Man-(1→2)-α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-α-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 8A1,2,3B1,3) = 3 β-D-mannose + N4-(α-D-Man-(1→3)-[α-D-Man-(1→3)-[α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-β-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2)By similarityEC:3.2.1.113By similarity
Cofactori
Ca2+By similarity, Mg2+By similarityNote: Ca2+. Can also use Mg2+, but with lower efficiency.By similarity
: protein glycosylation Pathwayi
This protein is involved in the pathway protein glycosylation, which is part of Protein modification.By similarityView all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 370 | Proton donorBy similarity | 1 | |
Metal bindingi | 501 | CalciumBy similarity | 1 |
GO - Molecular functioni
- calcium ion binding Source: InterPro
- mannosyl-oligosaccharide 1,2-alpha-mannosidase activity Source: UniProtKB-EC
GO - Biological processi
- carbohydrate metabolic process Source: UniProtKB-KW
- protein glycosylation Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Biological process | Carbohydrate metabolism |
Ligand | Metal-binding |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
Names & Taxonomyi
Protein namesi | Recommended name: Probable mannosyl-oligosaccharide alpha-1,2-mannosidase 1B (EC:3.2.1.113By similarity)Alternative name(s): Class I alpha-mannosidase 1B Man(9)-alpha-mannosidase 1B |
Gene namesi | Name:mns1B Synonyms:msdS ORF Names:ACLA_020870 |
Organismi | Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107) |
Taxonomic identifieri | 344612 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Aspergillaceae › Aspergillus › Aspergillus subgen. Fumigati › |
Proteomesi |
|
Organism-specific databases
VEuPathDBi | FungiDB:ACLA_020870 |
Subcellular locationi
Other locations
- Cytoplasmic vesicle lumen By similarity
Other locations
- cytoplasmic vesicle lumen Source: UniProtKB-SubCell
- membrane Source: InterPro
Keywords - Cellular componenti
Cytoplasmic vesiclePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 18 | Sequence analysisAdd BLAST | 18 | |
ChainiPRO_0000394816 | 19 – 511 | Probable mannosyl-oligosaccharide alpha-1,2-mannosidase 1BAdd BLAST | 493 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 90 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 177 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 327 ↔ 356 | By similarity | ||
Glycosylationi | 433 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
PRIDEi | A1CP08 |
Interactioni
Subunit structurei
Monomer.
By similarityProtein-protein interaction databases
STRINGi | 5057.CADACLAP00002234 |
Family & Domainsi
Sequence similaritiesi
Belongs to the glycosyl hydrolase 47 family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG2204, Eukaryota |
HOGENOMi | CLU_003818_0_2_1 |
OMAi | LIKMYLY |
OrthoDBi | 434316at2759 |
Family and domain databases
Gene3Di | 1.50.10.10, 1 hit |
InterProi | View protein in InterPro IPR012341, 6hp_glycosidase-like_sf IPR001382, Glyco_hydro_47 IPR036026, Seven-hairpin_glycosidases |
Pfami | View protein in Pfam PF01532, Glyco_hydro_47, 1 hit |
PRINTSi | PR00747, GLYHDRLASE47 |
SUPFAMi | SSF48225, SSF48225, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
A1CP08-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MHLPSLSLAW ALAGSSLALP QAEQPVLQDD RASSRAAAVK EAFSHAWDGY
60 70 80 90 100
KKYAFPHDEL HPISNGYGNS RNGWGASAVD ALSTAIVMRN ATIVHEILDH
110 120 130 140 150
IATIDYSKTS DTVSLFETTI RYLGGMLSGY DLLKGPAADL VKDTSKVDIL
160 170 180 190 200
LKQSKNLGDI LKFAFDTPSG VPYNNINITS HGHDGAKTNG LAVTGTLVLE
210 220 230 240 250
WTRLSDLTGD PEYAQLSQKA ESYLLHPQPS SAEPFPGLIG SNIDIKTGQF
260 270 280 290 300
TDGHVSWNGG DDSYYEYLIK MYVYDPTRFA LYRDRWVAAA ESSIEHLASH
310 320 330 340 350
PASRPDLTFL ATYDGGRYGL SSQHLACFDG GNFLLGGTVL DRRDLIDFGL
360 370 380 390 400
ALVDACHETY SQTLTGIGPE SFGWDANSVP ADQKELYERA GFYVQNGAYI
410 420 430 440 450
LRPEVIESFY YAWRVTGRSE YRDWVWTAFV AINKTCRTGS GYAGLTNVNA
460 470 480 490 500
ENGGGRYDNQ ESFLFAEVLK YAYLTHSEEL MAAEDAWQVQ RGDGNQFVFN
510
TEAHPIRVHH S
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DS027059 Genomic DNA Translation: EAW07379.1 |
RefSeqi | XP_001268805.1, XM_001268804.1 |
Genome annotation databases
EnsemblFungii | EAW07379; EAW07379; ACLA_020870 |
GeneIDi | 4701674 |
KEGGi | act:ACLA_020870 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DS027059 Genomic DNA Translation: EAW07379.1 |
RefSeqi | XP_001268805.1, XM_001268804.1 |
3D structure databases
AlphaFoldDBi | A1CP08 |
SMRi | A1CP08 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 5057.CADACLAP00002234 |
Proteomic databases
PRIDEi | A1CP08 |
Genome annotation databases
EnsemblFungii | EAW07379; EAW07379; ACLA_020870 |
GeneIDi | 4701674 |
KEGGi | act:ACLA_020870 |
Organism-specific databases
VEuPathDBi | FungiDB:ACLA_020870 |
Phylogenomic databases
eggNOGi | KOG2204, Eukaryota |
HOGENOMi | CLU_003818_0_2_1 |
OMAi | LIKMYLY |
OrthoDBi | 434316at2759 |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
Family and domain databases
Gene3Di | 1.50.10.10, 1 hit |
InterProi | View protein in InterPro IPR012341, 6hp_glycosidase-like_sf IPR001382, Glyco_hydro_47 IPR036026, Seven-hairpin_glycosidases |
Pfami | View protein in Pfam PF01532, Glyco_hydro_47, 1 hit |
PRINTSi | PR00747, GLYHDRLASE47 |
SUPFAMi | SSF48225, SSF48225, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MNS1B_ASPCL | |
Accessioni | A1CP08Primary (citable) accession number: A1CP08 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 15, 2010 |
Last sequence update: | January 23, 2007 | |
Last modified: | May 25, 2022 | |
This is version 74 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families