UniProtKB - A1CFK9 (PATB_ASPCL)
Carboxylesterase patB
patB
Functioni
Carboxylesterase; part of the gene cluster that mediates the biosynthesis of patulin, an acetate-derived tetraketide mycotoxin produced by several fungal species that shows antimicrobial properties against several bacteria (PubMed:19383676, PubMed:24334092).
The pathway begins with the synthesis of 6-methylsalicylic acid by the polyketide synthase (PKS) patK via condensation of acetate and malonate units (PubMed:19383676).
The 6-methylsalicylic acid decarboxylase patG then catalyzes the decarboxylation of 6-methylsalicylic acid to yield m-cresol (also known as 3-methylphenol) (PubMed:24334092).
These first reactions occur in the cytosol (By similarity).
The intermediate m-cresol is then transported into the endoplasmic reticulum where the cytochrome P450 monooxygenase patH converts it to m-hydroxybenzyl alcohol, which is further converted to gentisyl alcohol by the cytochrome P450 monooxygenase patI (By similarity).
The oxidoreductases patJ and patO further convert gentisyl alcohol to isoepoxydon in the vacuole (By similarity).
PatN catalyzes then the transformation of isoepoxydon into phyllostine (By similarity).
The cluster protein patF is responsible for the conversion from phyllostine to neopatulin whereas the alcohol dehydrogenase patD converts neopatulin to E-ascladiol (By similarity).
The steps between isoepoxydon and E-ascladiol occur in the cytosol, and E-ascladiol is probably secreted to the extracellular space by one of the cluster-specific transporters patC or patM (By similarity).
Finally, the secreted patulin synthase patE catalyzes the conversion of E-ascladiol to patulin (By similarity).
The role of the carboxylesterase patB in the patulin pathway was not determined yet and requires further study (By similarity).
By similarity2 PublicationsCatalytic activityi
- EC:3.1.1.1PROSITE-ProRule annotation
: patulin biosynthesis Pathwayi
This protein is involved in the pathway patulin biosynthesis, which is part of Mycotoxin biosynthesis.1 PublicationView all proteins of this organism that are known to be involved in the pathway patulin biosynthesis and in Mycotoxin biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 258 | Acyl-ester intermediatePROSITE-ProRule annotation | 1 | |
Binding sitei | 258 | SubstrateBy similarity | 1 | |
Active sitei | 380 | Charge relay systemBy similarity | 1 |
GO - Molecular functioni
- methyl indole-3-acetate esterase activity Source: UniProtKB-EC
Keywordsi
Molecular function | Hydrolase |
Enzyme and pathway databases
UniPathwayi | UPA00918 |
Protein family/group databases
ESTHERi | aspcl-a1cfk9, Fungal_carboxylesterase_lipase |
Names & Taxonomyi
Protein namesi | Recommended name: Carboxylesterase patB1 Publication (EC:3.1.1.11 Publication)Alternative name(s): Patulin synthesis protein B1 Publication |
Gene namesi | Name:patB1 Publication ORF Names:ACLA_093570 |
Organismi | Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107) |
Taxonomic identifieri | 344612 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Aspergillaceae › Aspergillus › Aspergillus subgen. Fumigati › |
Proteomesi |
|
Organism-specific databases
VEuPathDBi | FungiDB:ACLA_093570 |
Subcellular locationi
Cytoplasm and Cytosol
- cytosol By similarity
Cytosol
- cytosol Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Biotechnological usei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 16 | Sequence analysisAdd BLAST | 16 | |
ChainiPRO_5005120964 | 17 – 555 | Carboxylesterase patBAdd BLAST | 539 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 37 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation | 1 | |
Glycosylationi | 75 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation | 1 | |
Glycosylationi | 311 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation | 1 | |
Glycosylationi | 388 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation | 1 | |
Glycosylationi | 491 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation | 1 |
Keywords - PTMi
GlycoproteinFamily & Domainsi
Sequence similaritiesi
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG1516, Eukaryota |
HOGENOMi | CLU_006586_10_5_1 |
OMAi | FATAWAT |
OrthoDBi | 754103at2759 |
Family and domain databases
Gene3Di | 3.40.50.1820, 1 hit |
InterProi | View protein in InterPro IPR029058, AB_hydrolase IPR002018, CarbesteraseB IPR019826, Carboxylesterase_B_AS IPR019819, Carboxylesterase_B_CS |
Pfami | View protein in Pfam PF00135, COesterase, 1 hit |
SUPFAMi | SSF53474, SSF53474, 1 hit |
PROSITEi | View protein in PROSITE PS00122, CARBOXYLESTERASE_B_1, 1 hit PS00941, CARBOXYLESTERASE_B_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MLFTASLLLL LPWASAAPAN LPIVDLGYQR HQAISFNSTG QYYSFTNIRY
60 70 80 90 100
AEPPLGSRRF APPVAPHGRS KNIVNGTGLG YKCPQALACW FNVQNKFTSA
110 120 130 140 150
AAAGTPFDFN AAYEEVYTKD ACTEPAKQDP LQSEDCLFLD VYVPQDVWQK
160 170 180 190 200
GPQAAHQKGG APVLVYLQDG AYVGGSKSDQ NPAGLIARSR EEGSSGMIYV
210 220 230 240 250
GINYRLGVFG WLSGRKFTSS GGKPNAGLLD QRLALEWIQR HIHLFGGDPS
260 270 280 290 300
RVTVMGVSAG GGSIIMQMTA YGRGISPPFA QVITQSPAWE PGTKTPAIED
310 320 330 340 350
DLFDTFLASL NVTSLDQARR LPSHALTDAN YRLVASRPYG AGVLGPAIDG
360 370 380 390 400
DFIPDSPKRL LLQGKANPGV RVLTSYTAAE GFGIAPANIT DEASFQRYVG
410 420 430 440 450
LMLAGTDASV RTHAATVLYP AVFDGSMPYR TQHDRASLLW ADLAASCNTR
460 470 480 490 500
YLHAAVRTPG YAIEYSVPPA LHLSDTPSVF YNGPVADPTV NGTIAELLQR
510 520 530 540 550
QIVRFVKTGN PNGEPDPEVP VYDGRDLLDL GDDGVLVRPD STDNARCEYW
QKVHF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DS027052 Genomic DNA Translation: EAW11658.1 |
RefSeqi | XP_001273084.1, XM_001273083.1 |
Genome annotation databases
EnsemblFungii | EAW11658; EAW11658; ACLA_093570 |
GeneIDi | 4704864 |
KEGGi | act:ACLA_093570 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DS027052 Genomic DNA Translation: EAW11658.1 |
RefSeqi | XP_001273084.1, XM_001273083.1 |
3D structure databases
SMRi | A1CFK9 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 344612.A1CFK9 |
Protein family/group databases
ESTHERi | aspcl-a1cfk9, Fungal_carboxylesterase_lipase |
Genome annotation databases
EnsemblFungii | EAW11658; EAW11658; ACLA_093570 |
GeneIDi | 4704864 |
KEGGi | act:ACLA_093570 |
Organism-specific databases
VEuPathDBi | FungiDB:ACLA_093570 |
Phylogenomic databases
eggNOGi | KOG1516, Eukaryota |
HOGENOMi | CLU_006586_10_5_1 |
OMAi | FATAWAT |
OrthoDBi | 754103at2759 |
Enzyme and pathway databases
UniPathwayi | UPA00918 |
Family and domain databases
Gene3Di | 3.40.50.1820, 1 hit |
InterProi | View protein in InterPro IPR029058, AB_hydrolase IPR002018, CarbesteraseB IPR019826, Carboxylesterase_B_AS IPR019819, Carboxylesterase_B_CS |
Pfami | View protein in Pfam PF00135, COesterase, 1 hit |
SUPFAMi | SSF53474, SSF53474, 1 hit |
PROSITEi | View protein in PROSITE PS00122, CARBOXYLESTERASE_B_1, 1 hit PS00941, CARBOXYLESTERASE_B_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PATB_ASPCL | |
Accessioni | A1CFK9Primary (citable) accession number: A1CFK9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 7, 2016 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 71 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families