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Protein

Fap1 adhesin

Gene

fap1

Organism
Streptococcus parasanguinis
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The major structural element of fimbriae. Required for adherence to saliva-coated hydroxyapatite beads (SHA), an in vitro tooth model. A Fap1-dependent increase in adherence is seen as the pH is reduced from pH 8 to pH 5.2 Publications

Caution

It is uncertain whether Met-1 or Met-18 is the initiator.Curated

Names & Taxonomyi

Protein namesi
Recommended name:
Fap1 adhesin
Alternative name(s):
Fimbriae-associated protein Fap1
Serine-rich repeat protein Fap1
Gene namesi
Name:fap1
OrganismiStreptococcus parasanguinis
Taxonomic identifieri1318 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cell wall, Cytoplasm, Fimbrium, Secreted

Pathology & Biotechi

Disruption phenotypei

Loss of fimbriae on the cell surface, loss of adherence to saliva-coated hydroxyapatite beads (SAH), an in vitro tooth model.2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi219I → A: Loss of adherence to SHA. 1 Publication1
Mutagenesisi223E → A: Loss of adherence to SHA. 1 Publication1
Mutagenesisi227D → A: Loss of adherence to SHA. 1 Publication1
Mutagenesisi248L → A: Loss of adherence to SHA. 1 Publication1
Mutagenesisi249V → A: Loss of adherence to SHA. 1 Publication1
Mutagenesisi289E → A: Adheres to SHA. 1 Publication1
Mutagenesisi376 – 377IL → AA: Loss of adherence to SHA. 1 Publication2
Mutagenesisi385L → A: Loss of adherence to SHA. 1 Publication1
Mutagenesisi470L → A: Loss of adherence to SHA. 1 Publication1
Mutagenesisi488N → A: Loss of adherence to SHA. 1 Publication1
Mutagenesisi490Q → A: Loss of adherence to SHA. 1 Publication1
Mutagenesisi496I → A: Loss of adherence to SHA. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 851 PublicationAdd BLAST85
ChainiPRO_000041488286 – 2554Fap1 adhesinAdd BLAST2469
PropeptideiPRO_00004148832555 – 2587Removed by sortasePROSITE-ProRule annotationAdd BLAST33

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2554Pentaglycyl murein peptidoglycan amidated threoninePROSITE-ProRule annotation1

Post-translational modificationi

Glycosylated; occurs within the cytoplasm.2 Publications

Keywords - PTMi

Peptidoglycan-anchor

Expressioni

Inductioni

Low expression in early log phase, it increases until stationary phase (at protein level). A monocistronic transcript.2 Publications

Structurei

Secondary structure

12587
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SMRiA1C3L3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni107 – 195Ser-rich region 1Add BLAST89
Regioni191 – 522Sufficient to block adherence to beadsAdd BLAST332
Regioni516 – 2561Ser-rich region 2Add BLAST2046
Regioni2367 – 2587Required for localization to cell wall, fimbriae formation and adherence to saliva-coated hydroxyapatite beads (SHA) but not secretionAdd BLAST221

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi2551 – 2555LPXTG sorting signalPROSITE-ProRule annotation5

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi107 – 195Ser-richAdd BLAST89
Compositional biasi516 – 2561Ser-richAdd BLAST2046
Compositional biasi540 – 2548Glu-richAdd BLAST2009
Compositional biasi550 – 2510Val-richAdd BLAST1961

Domaini

Has short and long regions consisting of (E/V/I)S dipeptide repeats. These Ser-rich regions have 28 and 1000 repeats respectively.1 Publication

Sequence similaritiesi

Belongs to the SraP family.Curated

Keywords - Domaini

Signal

Family and domain databases

InterProiView protein in InterPro
IPR007383 DUF445
IPR019948 Gram-positive_anchor
IPR022263 KxYKxGKxW
IPR026465 Ser_adhes_glycop
PfamiView protein in Pfam
PF04286 DUF445, 1 hit
PF00746 Gram_pos_anchor, 1 hit
TIGRFAMsiTIGR03715 KxYKxGKxW, 1 hit
TIGR04224 ser_adhes_Nterm, 1 hit
PROSITEiView protein in PROSITE
PS50847 GRAM_POS_ANCHORING, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A1C3L3-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGKYKRAGET SRKTRVKMHK SGKNWVRTLI SQIGLMHFLG GSISEKKINV
60 70 80 90 100
DVYEQKNISA STILKGAVAL GALTGATVVS GNVFADETVL AKETTLTTTD
110 120 130 140 150
ANEVKLSSEN FDSEKAEEKI SLSQSESASE SVSESISESV SESVSTSESV
160 170 180 190 200
SESVSESVSE SISESVSESI SESISESVSE STSTSIVLSE SGAASGNKAT
210 220 230 240 250
SKGTEEKQDS VRENLDKMIS EAEVLNDMAA RKLITLDAEQ QLELMKSLVA
260 270 280 290 300
TQSQLEATKN LIGDPNATVA DLQIAYTTLG NNTQALGNEL IKLNPNGQIY
310 320 330 340 350
AVLNNTEASR AATLRSTTTG TKTTFTISDF SNGGTQYYWA GGNANNLKNP
360 370 380 390 400
ISSISAVYDS ATGKISWTVE YDPTTILKSP ALKTLKTYTG IYIDTSSDSK
410 420 430 440 450
LSTPTNVLID GAATNPVTNF YGNGSKGIEY VSKGTTKGVT KHTITFDTAF
460 470 480 490 500
SGRANDLADL KIKMLAATTL SDPHFYEDGS KGNYGRYNGQ TAPYVIANDS
510 520 530 540 550
GTAIGGYQVS GVNADSIPSD TTSQSESTSK SESTSKSISE SVIESISESV
560 570 580 590 600
IGSVSESVSE SVSESVSESI TESVSESVSE SISESVSESV SESISESVSE
610 620 630 640 650
SVSESISESV SESVSESISE SVSESVSESI SESISESVSE SVSESISESV
660 670 680 690 700
SESVSESISE SVSESVSESI SESVSESVSE SISESVSESV SESISESVSE
710 720 730 740 750
SVSESISESV SESVSESISE SVSESVSESI SESVSESVSE SISESVSESV
760 770 780 790 800
SESISESVSE SVSESISESV SESVSESVSE SVSESVSESI SESVSESVSE
810 820 830 840 850
SVSESVSESV SESVSESISE SVSESVSESI SESVSESVSE SISESVSESV
860 870 880 890 900
SESISESVSE SVSESISESV SESVSESISE SVSESVSESI SESVSESVSE
910 920 930 940 950
SISESVSESV SESISESVSE SVSESISESV SESVSESVSE SVSESVSESV
960 970 980 990 1000
SESVSESISE SVSESVSESI SESVSESVSE SISESVSESV SESISESVSE
1010 1020 1030 1040 1050
SVSESISESV SESVSESVSE SVSESVSESV SESVSESISE SVSESVSESV
1060 1070 1080 1090 1100
SESISESVSE SVSESISESV SESVSESISE SVSESVSESV SESVSESVSE
1110 1120 1130 1140 1150
SISESVSESV SESISESVSE SVSESISESV SESVSESISE SVSESVSESI
1160 1170 1180 1190 1200
SESVSESVSE SISESVSESV SESVSESVSE SVSESISESV SESVSESISE
1210 1220 1230 1240 1250
SVSESVSESV SESISESVSE SVSESISESV SESVSESISE SVSESVSESI
1260 1270 1280 1290 1300
SESVSESVSE SVSESVSESV SESVSESVSE SVSESVSESI SESVSESVSE
1310 1320 1330 1340 1350
SVSESISESV SESVSESISE SVSESVSESI SESVSESVSE SISESVSESV
1360 1370 1380 1390 1400
SESISESVSE SVSESISESV SESVSESVSE SVSESVSESI SESVSESVSE
1410 1420 1430 1440 1450
SISESVSESV SESISESVSE SVSESISESV SESVSESVSE SISESVSESV
1460 1470 1480 1490 1500
SESISESVSE SVSESISESV SESVSESVSE SVSESVSESV SESVSESVSE
1510 1520 1530 1540 1550
SISESVSESV SESISESVSE SVSESISESV SESVSESISE SVSESVSESI
1560 1570 1580 1590 1600
SESVSESVSE SISESVSESV SESISESVSE SVSESVSESV SESVSESISE
1610 1620 1630 1640 1650
SVSESVSESI SESVSESVSE SVSESVSESV SESVSESVSE SVSESISESV
1660 1670 1680 1690 1700
SESVSESVSE SVSESVSESI SESVSESVSE SISESVSESV SESISESVSE
1710 1720 1730 1740 1750
SVSESISESV SESVSESISE SVSESVSESI SESVSESVSE SISESVSESV
1760 1770 1780 1790 1800
SESVSESVSE SVSESVSESV SESISESVSE SVSESISESV SESVSESISE
1810 1820 1830 1840 1850
SVSESVSESV SESVSESVSE SISESVSESV SESISESVSE SVSESISESV
1860 1870 1880 1890 1900
SESVSESISE SVSESVSESV SESISESVSE SVSESISESV SESVSESISE
1910 1920 1930 1940 1950
SVSESVSESI SESVSESVSE SISESVSESV SESISESVSE SVSESVSESV
1960 1970 1980 1990 2000
SESVSESISE SVSESISESV SESVSESISE SVSESVSESI SESVSESVSE
2010 2020 2030 2040 2050
SISESVSESV SESISESVSE SVSESISESV SESVSESISE SVSESVSESI
2060 2070 2080 2090 2100
SESVSESVSE SVSESISESV SESVSESISE SVSESVSESI SESVSESVSE
2110 2120 2130 2140 2150
SVSESVSESV SESVSESVSE SVSESISESV SESVSESISE SVSESVSESI
2160 2170 2180 2190 2200
SESVSESVSE SISESVSESV SESISESVSE SVSESISESV SESVSESISE
2210 2220 2230 2240 2250
SVSESVSESV SESVSESVSE SISESVSESV SESVSESISE SVSESVSESI
2260 2270 2280 2290 2300
SESVSESVSE SVSESVSESV SESVSESVSE SVSESISESV SESVSESVSE
2310 2320 2330 2340 2350
SVSESVSESI SESVSESVSE SISESVSESV SESISESVSE SVSESISESV
2360 2370 2380 2390 2400
SESVSESISE SVSESVSESI SESVSESVSE SISESVSESV SESISESVSE
2410 2420 2430 2440 2450
SVSESVSESI SESVSESVSE SISESVSESV SESISESVSE SVSESISESV
2460 2470 2480 2490 2500
SESVSESVSE SVSESVSESV SESVSESISE SVSESVSESI SESVSESVSE
2510 2520 2530 2540 2550
SVSESVSESV SESISESVSE SISESVSESV SESISESVSE SVSESISERT
2560 2570 2580
LPNTGENVSS SLGLVGLSGL LFGALLGRKK RKSEDAE
Length:2,587
Mass (Da):265,063
Last modified:January 23, 2007 - v1
Checksum:iC8AD6E9F6EE8FE5A
GO

Sequence cautioni

The sequence AAC79868 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti461K → E in AAC79868 (PubMed:10594831).Curated1
Sequence conflicti528T → A in AAC79868 (PubMed:10594831).Curated1
Sequence conflicti1146V → P in AAC79868 (PubMed:10594831).Curated1
Sequence conflicti1226I → S in AAC79868 (PubMed:10594831).Curated1
Sequence conflicti1830V → M in AAC79868 (PubMed:10594831).Curated1
Sequence conflicti1861S → P in AAC79868 (PubMed:10594831).Curated1
Sequence conflicti1885S → T in AAC79868 (PubMed:10594831).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF100426 Genomic DNA Translation: AAC79868.1 Different initiation.
DQ990875 Genomic DNA Translation: ABL73998.1
PIRiT17451

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF100426 Genomic DNA Translation: AAC79868.1 Different initiation.
DQ990875 Genomic DNA Translation: ABL73998.1
PIRiT17451

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2KUBNMR-A213-293[»]
2X12X-ray2.90A/B191-522[»]
3RGUX-ray3.00A/B/C/D201-316[»]
SMRiA1C3L3
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiView protein in InterPro
IPR007383 DUF445
IPR019948 Gram-positive_anchor
IPR022263 KxYKxGKxW
IPR026465 Ser_adhes_glycop
PfamiView protein in Pfam
PF04286 DUF445, 1 hit
PF00746 Gram_pos_anchor, 1 hit
TIGRFAMsiTIGR03715 KxYKxGKxW, 1 hit
TIGR04224 ser_adhes_Nterm, 1 hit
PROSITEiView protein in PROSITE
PS50847 GRAM_POS_ANCHORING, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiFAP1_STRPA
AccessioniPrimary (citable) accession number: A1C3L3
Secondary accession number(s): Q9ZFF9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: January 23, 2007
Last modified: February 28, 2018
This is version 41 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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