UniProtKB - A1A6H3 (RBSK_ARATH)
Ribokinase
RBSK
Functioni
Catalyzes the phosphorylation of ribose at O-5 in a reaction requiring ATP and magnesium. The resulting D-ribose-5-phosphate can then be used either for sythesis of nucleotides, histidine, and tryptophan, or as a component of the pentose phosphate pathway (By similarity) (PubMed:27601466).
Can also use xylose and fructose as carbohydrate substrates with a low efficiency (PubMed:27601466).
Can use GTP, and, to a lower extent, CTP and UTP as alternative phosphoryl donors (PubMed:27601466).
UniRule annotation1 PublicationCaution
Catalytic activityi
- EC:2.7.1.15UniRule annotation1 Publication
Cofactori
Activity regulationi
Kineticsi
- KM=150 µM for D-ribose1 Publication
- KM=45 µM for ATP1 Publication
: D-ribose degradation Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes D-ribose 5-phosphate from beta-D-ribopyranose.UniRule annotation This subpathway is part of the pathway D-ribose degradation, which is itself part of Carbohydrate metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-ribose 5-phosphate from beta-D-ribopyranose, the pathway D-ribose degradation and in Carbohydrate metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 210 | SubstrateUniRule annotation | 1 | |
Binding sitei | 255 | ATPUniRule annotation | 1 | |
Metal bindingi | 319 | PotassiumUniRule annotation | 1 | |
Metal bindingi | 321 | Potassium; via carbonyl oxygenUniRule annotation | 1 | |
Active sitei | 325 | Proton acceptorUniRule annotation | 1 | |
Binding sitei | 325 | SubstrateUniRule annotation | 1 | |
Metal bindingi | 355 | Potassium; via carbonyl oxygenUniRule annotation | 1 | |
Metal bindingi | 358 | Potassium; via carbonyl oxygenUniRule annotation | 1 | |
Metal bindingi | 360 | Potassium; via carbonyl oxygenUniRule annotation | 1 | |
Metal bindingi | 364 | PotassiumUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 291 – 296 | ATPUniRule annotation | 6 | |
Nucleotide bindingi | 324 – 325 | ATPUniRule annotation | 2 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- metal ion binding Source: UniProtKB-KW
- ribokinase activity Source: TAIR
GO - Biological processi
- D-ribose catabolic process Source: UniProtKB-UniRule
- nucleoside metabolic process Source: TAIR
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Carbohydrate metabolism |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium |
Enzyme and pathway databases
BioCyci | ARA:AT1G17160-MONOMER |
UniPathwayi | UPA00916;UER00889 |
Names & Taxonomyi
Protein namesi | Recommended name: RibokinaseUniRule annotation (EC:2.7.1.15UniRule annotation1 Publication)Short name: AtRBSK1 Publication Short name: RKUniRule annotation |
Gene namesi | Name:RBSK1 Publication Ordered Locus Names:At1g17160Imported ORF Names:F20D23.14Imported |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT1G17160 |
TAIRi | locus:2020337, AT1G17160 |
Subcellular locationi
Chloroplast
- chloroplast nucleoid 1 Publication
Chloroplast
- chloroplast Source: TAIR
- chloroplast nucleoid Source: UniProtKB-SubCell
- chloroplast stroma Source: TAIR
Nucleus
- nucleus Source: UniProtKB-UniRule
Other locations
- plastid nucleoid Source: TAIR
Keywords - Cellular componenti
Chloroplast, PlastidPathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 1 – 74 | Missing : Loss of ribokinase activity. 1 PublicationAdd BLAST | 74 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – ? | Chloroplast1 Publication | ||
ChainiPRO_0000446983 | ? – 379 | Ribokinase |
Proteomic databases
PaxDbi | A1A6H3 |
PRIDEi | A1A6H3 |
ProteomicsDBi | 181456 |
Interactioni
Subunit structurei
Homodimer.
UniRule annotationProtein-protein interaction databases
STRINGi | 3702.AT1G17160.1 |
Structurei
Secondary structure
3D structure databases
SMRi | A1A6H3 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 78 – 80 | Substrate bindingUniRule annotation | 3 | |
Regioni | 106 – 110 | Substrate bindingUniRule annotation | 5 |
Sequence similaritiesi
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG2855, Eukaryota |
HOGENOMi | CLU_027634_2_2_1 |
OMAi | DIVLIQQ |
OrthoDBi | 918144at2759 |
PhylomeDBi | A1A6H3 |
Family and domain databases
CDDi | cd01174, ribokinase, 1 hit |
Gene3Di | 3.40.1190.20, 1 hit |
HAMAPi | MF_01987, Ribokinase, 1 hit |
InterProi | View protein in InterPro IPR002173, Carboh/pur_kinase_PfkB_CS IPR011877, D_ribokin IPR011611, PfkB_dom IPR002139, Ribo/fructo_kinase IPR029056, Ribokinase-like |
Pfami | View protein in Pfam PF00294, PfkB, 1 hit |
PRINTSi | PR00990, RIBOKINASE |
SUPFAMi | SSF53613, SSF53613, 1 hit |
PROSITEi | View protein in PROSITE PS00584, PFKB_KINASES_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MMKGISSVSQ SINYNPYIEF NRPQLQISTV NPNPAQSRFS RPRSLRVLSL
60 70 80 90 100
SADPSANRNP KSAVDAHAPP LVVVGSANAD IYVEIERLPK EGETISAKTG
110 120 130 140 150
QTLAGGKGAN QAACGAKLMY PTYFVGRLGE DAHGKLIAEA LGDDGCGVHL
160 170 180 190 200
DYVRSVNNEP TGHAVVMLQS DGQNSIIIVG GANMKAWPEI MSDDDLEIVR
210 220 230 240 250
NAGIVLLQRE IPDSINIQVA KAVKKAGVPV ILDVGGMDTP IPNELLDSID
260 270 280 290 300
ILSPNETELS RLTGMPTETF EQISQAVAKC HKLGVKQVLV KLGSKGSALF
310 320 330 340 350
IQGEKPIQQS IIPAAQVVDT TGAGDTFTAA FAVAMVEGKS HEECLRFAAA
360 370
AASLCVQVKG AIPSMPDRKS VLKLLKFSI
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA8MQX7 | A8MQX7_ARATH | Ribokinase | At1g17160, F20D23.14, F20D23_14 | 355 | Annotation score: |
Sequence cautioni
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC007651 Genomic DNA Translation: AAD50017.1 Different initiation. CP002684 Genomic DNA Translation: AEE29552.1 BT029492 mRNA Translation: ABL66749.1 |
PIRi | F86307 |
RefSeqi | NP_173159.1, NM_101577.5 |
Genome annotation databases
EnsemblPlantsi | AT1G17160.1; AT1G17160.1; AT1G17160 |
GeneIDi | 838287 |
Gramenei | AT1G17160.1; AT1G17160.1; AT1G17160 |
KEGGi | ath:AT1G17160 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC007651 Genomic DNA Translation: AAD50017.1 Different initiation. CP002684 Genomic DNA Translation: AEE29552.1 BT029492 mRNA Translation: ABL66749.1 |
PIRi | F86307 |
RefSeqi | NP_173159.1, NM_101577.5 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6ILR | X-ray | 1.97 | A/B | 68-379 | [»] | |
6ILS | X-ray | 1.80 | A/B | 68-379 | [»] | |
6ILT | X-ray | 2.20 | A/B | 68-379 | [»] | |
SMRi | A1A6H3 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 3702.AT1G17160.1 |
Proteomic databases
PaxDbi | A1A6H3 |
PRIDEi | A1A6H3 |
ProteomicsDBi | 181456 |
Genome annotation databases
EnsemblPlantsi | AT1G17160.1; AT1G17160.1; AT1G17160 |
GeneIDi | 838287 |
Gramenei | AT1G17160.1; AT1G17160.1; AT1G17160 |
KEGGi | ath:AT1G17160 |
Organism-specific databases
Araporti | AT1G17160 |
TAIRi | locus:2020337, AT1G17160 |
Phylogenomic databases
eggNOGi | KOG2855, Eukaryota |
HOGENOMi | CLU_027634_2_2_1 |
OMAi | DIVLIQQ |
OrthoDBi | 918144at2759 |
PhylomeDBi | A1A6H3 |
Enzyme and pathway databases
UniPathwayi | UPA00916;UER00889 |
BioCyci | ARA:AT1G17160-MONOMER |
Miscellaneous databases
PROi | PR:A1A6H3 |
Gene expression databases
ExpressionAtlasi | A1A6H3, baseline and differential |
Family and domain databases
CDDi | cd01174, ribokinase, 1 hit |
Gene3Di | 3.40.1190.20, 1 hit |
HAMAPi | MF_01987, Ribokinase, 1 hit |
InterProi | View protein in InterPro IPR002173, Carboh/pur_kinase_PfkB_CS IPR011877, D_ribokin IPR011611, PfkB_dom IPR002139, Ribo/fructo_kinase IPR029056, Ribokinase-like |
Pfami | View protein in Pfam PF00294, PfkB, 1 hit |
PRINTSi | PR00990, RIBOKINASE |
SUPFAMi | SSF53613, SSF53613, 1 hit |
PROSITEi | View protein in PROSITE PS00584, PFKB_KINASES_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RBSK_ARATH | |
Accessioni | A1A6H3Primary (citable) accession number: A1A6H3 Secondary accession number(s): Q9SHH5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 8, 2019 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 122 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families